The Experts below are selected from a list of 294 Experts worldwide ranked by ideXlab platform
Ernst Reichmann - One of the best experts on this subject based on the ideXlab platform.
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de novo epidermal regeneration using human eccrine Sweat Gland cells higher competence of secretory over absorptive cells
Journal of Investigative Dermatology, 2014Co-Authors: Luca Pontiggia, Thomas Biedermann, Sophie Bottcherhaberzeth, Erik Braziulis, Martin Meuli, Claudia Meulisimmen, Carol Oliveira, Agnieszka S Klar, Ernst ReichmannAbstract:In our previous work, we showed that human Sweat Gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of Sweat Gland cells in epidermal regeneration and maintenance remained unclear. In this study, we compare the regenerative potential of both secretory and absorptive Sweat Gland cell subpopulations. We demonstrate the superiority of secretory over absorptive cells in forming a new epidermis on two levels: first, the proliferative and colony-forming efficiencies in vitro are significantly higher for secretory cells (SCs), and second, SCs show a higher frequency of successful epidermis formation as well as an increase in the thickness of the formed epidermis in the in vitro and in vivo functional analyses using a 3D dermo-epidermal skin model. However, the ability of forming functional skin substitutes is not limited to SCs, which supports the hypothesis that multiple subtypes of Sweat Gland epithelial cells hold regenerative properties, while the existence and exact localization of a keratinocyte stem cell population in the human eccrine Sweat Gland remain elusive.
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human eccrine Sweat Gland cells can reconstitute a stratified epidermis
Journal of Investigative Dermatology, 2010Co-Authors: Thomas Biedermann, Luca Pontiggia, Sophie Bottcherhaberzeth, Sasha J Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst ReichmannAbstract:Eccrine Sweat Glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine Sweat Gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine Sweat Gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10–12 cell layers is formed by Sweat Gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the Sweat Gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the Sweat Gland-derived epidermis. These data suggest that human eccrine Sweat Glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine Sweat Gland cells.
Martin Meuli - One of the best experts on this subject based on the ideXlab platform.
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de novo epidermal regeneration using human eccrine Sweat Gland cells higher competence of secretory over absorptive cells
Journal of Investigative Dermatology, 2014Co-Authors: Luca Pontiggia, Thomas Biedermann, Sophie Bottcherhaberzeth, Erik Braziulis, Martin Meuli, Claudia Meulisimmen, Carol Oliveira, Agnieszka S Klar, Ernst ReichmannAbstract:In our previous work, we showed that human Sweat Gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of Sweat Gland cells in epidermal regeneration and maintenance remained unclear. In this study, we compare the regenerative potential of both secretory and absorptive Sweat Gland cell subpopulations. We demonstrate the superiority of secretory over absorptive cells in forming a new epidermis on two levels: first, the proliferative and colony-forming efficiencies in vitro are significantly higher for secretory cells (SCs), and second, SCs show a higher frequency of successful epidermis formation as well as an increase in the thickness of the formed epidermis in the in vitro and in vivo functional analyses using a 3D dermo-epidermal skin model. However, the ability of forming functional skin substitutes is not limited to SCs, which supports the hypothesis that multiple subtypes of Sweat Gland epithelial cells hold regenerative properties, while the existence and exact localization of a keratinocyte stem cell population in the human eccrine Sweat Gland remain elusive.
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human eccrine Sweat Gland cells turn into melanin uptaking keratinocytes in dermo epidermal skin substitutes
Journal of Investigative Dermatology, 2013Co-Authors: Sophie Bottcherhaberzeth, Thomas Biedermann, Luca Pontiggia, Erik Braziulis, Clemens Schiestl, Bart Hendriks, Ossia M Eichhoff, Daniel S Widmer, Claudia Meulisimmen, Martin MeuliAbstract:Recently, Biedermann et al. (2010) have demonstrated that human eccrine Sweat Gland cells can develop a multilayered epidermis. The question still remains whether these cells can fulfill exclusive and very specific functional properties of epidermal keratinocytes, such as the incorporation of melanin, a feature absent in Sweat Gland cells. We added human melanocytes to eccrine Sweat Gland cells to let them develop into an epidermal analog in vivo. The interaction between melanocytes and Sweat Gland–derived keratinocytes was investigated. The following results were gained: (1) macroscopically, a pigmentation of the substitutes was seen 2–3 weeks after transplantation; (2) we confirmed the development of a multilayered, stratified epidermis with melanocytes distributed evenly throughout the basal layer; (3) melanocytic dendrites projected to suprabasal layers; and (4) melanin was observed to be integrated into former eccrine Sweat Gland cells. These skin substitutes were similar or equal to skin substitutes cultured from human epidermal keratinocytes. The only differences observed were a delay in pigmentation and less melanin uptake. These data suggest that eccrine Sweat Gland cells can form a functional epidermal melanin unit, thereby providing striking evidence that they can assume one of the most characteristic keratinocyte properties.
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human eccrine Sweat Gland cells can reconstitute a stratified epidermis
Journal of Investigative Dermatology, 2010Co-Authors: Thomas Biedermann, Luca Pontiggia, Sophie Bottcherhaberzeth, Sasha J Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst ReichmannAbstract:Eccrine Sweat Glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine Sweat Gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine Sweat Gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10–12 cell layers is formed by Sweat Gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the Sweat Gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the Sweat Gland-derived epidermis. These data suggest that human eccrine Sweat Glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine Sweat Gland cells.
Thomas Biedermann - One of the best experts on this subject based on the ideXlab platform.
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de novo epidermal regeneration using human eccrine Sweat Gland cells higher competence of secretory over absorptive cells
Journal of Investigative Dermatology, 2014Co-Authors: Luca Pontiggia, Thomas Biedermann, Sophie Bottcherhaberzeth, Erik Braziulis, Martin Meuli, Claudia Meulisimmen, Carol Oliveira, Agnieszka S Klar, Ernst ReichmannAbstract:In our previous work, we showed that human Sweat Gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of Sweat Gland cells in epidermal regeneration and maintenance remained unclear. In this study, we compare the regenerative potential of both secretory and absorptive Sweat Gland cell subpopulations. We demonstrate the superiority of secretory over absorptive cells in forming a new epidermis on two levels: first, the proliferative and colony-forming efficiencies in vitro are significantly higher for secretory cells (SCs), and second, SCs show a higher frequency of successful epidermis formation as well as an increase in the thickness of the formed epidermis in the in vitro and in vivo functional analyses using a 3D dermo-epidermal skin model. However, the ability of forming functional skin substitutes is not limited to SCs, which supports the hypothesis that multiple subtypes of Sweat Gland epithelial cells hold regenerative properties, while the existence and exact localization of a keratinocyte stem cell population in the human eccrine Sweat Gland remain elusive.
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human eccrine Sweat Gland cells turn into melanin uptaking keratinocytes in dermo epidermal skin substitutes
Journal of Investigative Dermatology, 2013Co-Authors: Sophie Bottcherhaberzeth, Thomas Biedermann, Luca Pontiggia, Erik Braziulis, Clemens Schiestl, Bart Hendriks, Ossia M Eichhoff, Daniel S Widmer, Claudia Meulisimmen, Martin MeuliAbstract:Recently, Biedermann et al. (2010) have demonstrated that human eccrine Sweat Gland cells can develop a multilayered epidermis. The question still remains whether these cells can fulfill exclusive and very specific functional properties of epidermal keratinocytes, such as the incorporation of melanin, a feature absent in Sweat Gland cells. We added human melanocytes to eccrine Sweat Gland cells to let them develop into an epidermal analog in vivo. The interaction between melanocytes and Sweat Gland–derived keratinocytes was investigated. The following results were gained: (1) macroscopically, a pigmentation of the substitutes was seen 2–3 weeks after transplantation; (2) we confirmed the development of a multilayered, stratified epidermis with melanocytes distributed evenly throughout the basal layer; (3) melanocytic dendrites projected to suprabasal layers; and (4) melanin was observed to be integrated into former eccrine Sweat Gland cells. These skin substitutes were similar or equal to skin substitutes cultured from human epidermal keratinocytes. The only differences observed were a delay in pigmentation and less melanin uptake. These data suggest that eccrine Sweat Gland cells can form a functional epidermal melanin unit, thereby providing striking evidence that they can assume one of the most characteristic keratinocyte properties.
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human eccrine Sweat Gland cells can reconstitute a stratified epidermis
Journal of Investigative Dermatology, 2010Co-Authors: Thomas Biedermann, Luca Pontiggia, Sophie Bottcherhaberzeth, Sasha J Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst ReichmannAbstract:Eccrine Sweat Glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine Sweat Gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine Sweat Gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10–12 cell layers is formed by Sweat Gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the Sweat Gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the Sweat Gland-derived epidermis. These data suggest that human eccrine Sweat Glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine Sweat Gland cells.
Luca Pontiggia - One of the best experts on this subject based on the ideXlab platform.
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de novo epidermal regeneration using human eccrine Sweat Gland cells higher competence of secretory over absorptive cells
Journal of Investigative Dermatology, 2014Co-Authors: Luca Pontiggia, Thomas Biedermann, Sophie Bottcherhaberzeth, Erik Braziulis, Martin Meuli, Claudia Meulisimmen, Carol Oliveira, Agnieszka S Klar, Ernst ReichmannAbstract:In our previous work, we showed that human Sweat Gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of Sweat Gland cells in epidermal regeneration and maintenance remained unclear. In this study, we compare the regenerative potential of both secretory and absorptive Sweat Gland cell subpopulations. We demonstrate the superiority of secretory over absorptive cells in forming a new epidermis on two levels: first, the proliferative and colony-forming efficiencies in vitro are significantly higher for secretory cells (SCs), and second, SCs show a higher frequency of successful epidermis formation as well as an increase in the thickness of the formed epidermis in the in vitro and in vivo functional analyses using a 3D dermo-epidermal skin model. However, the ability of forming functional skin substitutes is not limited to SCs, which supports the hypothesis that multiple subtypes of Sweat Gland epithelial cells hold regenerative properties, while the existence and exact localization of a keratinocyte stem cell population in the human eccrine Sweat Gland remain elusive.
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human eccrine Sweat Gland cells turn into melanin uptaking keratinocytes in dermo epidermal skin substitutes
Journal of Investigative Dermatology, 2013Co-Authors: Sophie Bottcherhaberzeth, Thomas Biedermann, Luca Pontiggia, Erik Braziulis, Clemens Schiestl, Bart Hendriks, Ossia M Eichhoff, Daniel S Widmer, Claudia Meulisimmen, Martin MeuliAbstract:Recently, Biedermann et al. (2010) have demonstrated that human eccrine Sweat Gland cells can develop a multilayered epidermis. The question still remains whether these cells can fulfill exclusive and very specific functional properties of epidermal keratinocytes, such as the incorporation of melanin, a feature absent in Sweat Gland cells. We added human melanocytes to eccrine Sweat Gland cells to let them develop into an epidermal analog in vivo. The interaction between melanocytes and Sweat Gland–derived keratinocytes was investigated. The following results were gained: (1) macroscopically, a pigmentation of the substitutes was seen 2–3 weeks after transplantation; (2) we confirmed the development of a multilayered, stratified epidermis with melanocytes distributed evenly throughout the basal layer; (3) melanocytic dendrites projected to suprabasal layers; and (4) melanin was observed to be integrated into former eccrine Sweat Gland cells. These skin substitutes were similar or equal to skin substitutes cultured from human epidermal keratinocytes. The only differences observed were a delay in pigmentation and less melanin uptake. These data suggest that eccrine Sweat Gland cells can form a functional epidermal melanin unit, thereby providing striking evidence that they can assume one of the most characteristic keratinocyte properties.
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human eccrine Sweat Gland cells can reconstitute a stratified epidermis
Journal of Investigative Dermatology, 2010Co-Authors: Thomas Biedermann, Luca Pontiggia, Sophie Bottcherhaberzeth, Sasha J Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst ReichmannAbstract:Eccrine Sweat Glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine Sweat Gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine Sweat Gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10–12 cell layers is formed by Sweat Gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the Sweat Gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the Sweat Gland-derived epidermis. These data suggest that human eccrine Sweat Glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine Sweat Gland cells.
Sophie Bottcherhaberzeth - One of the best experts on this subject based on the ideXlab platform.
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de novo epidermal regeneration using human eccrine Sweat Gland cells higher competence of secretory over absorptive cells
Journal of Investigative Dermatology, 2014Co-Authors: Luca Pontiggia, Thomas Biedermann, Sophie Bottcherhaberzeth, Erik Braziulis, Martin Meuli, Claudia Meulisimmen, Carol Oliveira, Agnieszka S Klar, Ernst ReichmannAbstract:In our previous work, we showed that human Sweat Gland-derived epithelial cells represent an alternative source of keratinocytes to grow a near normal autologous epidermis. The role of subtypes of Sweat Gland cells in epidermal regeneration and maintenance remained unclear. In this study, we compare the regenerative potential of both secretory and absorptive Sweat Gland cell subpopulations. We demonstrate the superiority of secretory over absorptive cells in forming a new epidermis on two levels: first, the proliferative and colony-forming efficiencies in vitro are significantly higher for secretory cells (SCs), and second, SCs show a higher frequency of successful epidermis formation as well as an increase in the thickness of the formed epidermis in the in vitro and in vivo functional analyses using a 3D dermo-epidermal skin model. However, the ability of forming functional skin substitutes is not limited to SCs, which supports the hypothesis that multiple subtypes of Sweat Gland epithelial cells hold regenerative properties, while the existence and exact localization of a keratinocyte stem cell population in the human eccrine Sweat Gland remain elusive.
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human eccrine Sweat Gland cells turn into melanin uptaking keratinocytes in dermo epidermal skin substitutes
Journal of Investigative Dermatology, 2013Co-Authors: Sophie Bottcherhaberzeth, Thomas Biedermann, Luca Pontiggia, Erik Braziulis, Clemens Schiestl, Bart Hendriks, Ossia M Eichhoff, Daniel S Widmer, Claudia Meulisimmen, Martin MeuliAbstract:Recently, Biedermann et al. (2010) have demonstrated that human eccrine Sweat Gland cells can develop a multilayered epidermis. The question still remains whether these cells can fulfill exclusive and very specific functional properties of epidermal keratinocytes, such as the incorporation of melanin, a feature absent in Sweat Gland cells. We added human melanocytes to eccrine Sweat Gland cells to let them develop into an epidermal analog in vivo. The interaction between melanocytes and Sweat Gland–derived keratinocytes was investigated. The following results were gained: (1) macroscopically, a pigmentation of the substitutes was seen 2–3 weeks after transplantation; (2) we confirmed the development of a multilayered, stratified epidermis with melanocytes distributed evenly throughout the basal layer; (3) melanocytic dendrites projected to suprabasal layers; and (4) melanin was observed to be integrated into former eccrine Sweat Gland cells. These skin substitutes were similar or equal to skin substitutes cultured from human epidermal keratinocytes. The only differences observed were a delay in pigmentation and less melanin uptake. These data suggest that eccrine Sweat Gland cells can form a functional epidermal melanin unit, thereby providing striking evidence that they can assume one of the most characteristic keratinocyte properties.
-
human eccrine Sweat Gland cells can reconstitute a stratified epidermis
Journal of Investigative Dermatology, 2010Co-Authors: Thomas Biedermann, Luca Pontiggia, Sophie Bottcherhaberzeth, Sasha J Tharakan, Erik Braziulis, Clemens Schiestl, Martin Meuli, Ernst ReichmannAbstract:Eccrine Sweat Glands are generally considered to be a possible epidermal stem cell source. Here we compared the multilayered epithelia formed by epidermal keratinocytes and those formed by eccrine Sweat Gland cells. We demonstrated both in vitro and in vivo the capability of human eccrine Sweat Gland cells to form a stratified interfollicular epidermis substitute on collagen hydrogels. This is substantiated by the following findings: (1) a stratified epidermis consisting of 10–12 cell layers is formed by Sweat Gland cells; (2) a distinct stratum corneum develops and is maintained after transplantation onto immuno-incompetent rats; (3) proteins such as filaggrin, loricrin, involucrin, envoplakin, periplakin, and transglutaminases I and III match with the pattern of the normal human skin; (4) junctional complexes and hemidesmosomes are readily and regularly established; (5) cell proliferation in the basal layer reaches homeostatic levels; (6) the Sweat Gland-derived epidermis is anchored by hemidesmosomes within a well-developed basal lamina; and (7) palmo-plantar or mucosal markers are not expressed in the Sweat Gland-derived epidermis. These data suggest that human eccrine Sweat Glands are an additional source of keratinocytes that can generate a stratified epidermis. Our findings raise the question of the extent to which the human skin is repaired and/or permanently renewed by eccrine Sweat Gland cells.
