The Experts below are selected from a list of 3603 Experts worldwide ranked by ideXlab platform
Hanfa Zou - One of the best experts on this subject based on the ideXlab platform.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis.
Journal of chromatography. A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (muHPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 microL on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis
Journal of Chromatography A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. © 2007 Elsevier B.V. All rights reserved.
Fangjun Wang - One of the best experts on this subject based on the ideXlab platform.
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Online Sample Injection and Multidimensional Chromatography Separation by Using Strong-Cation Exchange Monolithic Column
Springer Theses, 2013Co-Authors: Fangjun WangAbstract:Nanoflow LC-MS/MS is the central platform in shotgun proteome analysis due to its high sensitivity and high separation capability. The flow rate of nanoflow LC separation is usually 50–300 nL/min. Manual injection of protein sample onto a 50–100 μm inner diameter (i.d.) capillary LC separation column by nitrogen pressure or void capillary is feasibly achieved in previous proteome analyses. However, this strategy is time-consuming, labor-intensive, and variations are easily introduced in the manual operations, which significantly decreases the analysis throughput and reproducibility. Automated sample injection by using a trap column with shorter column length and larger inner diameter is the most popular way in nanoflow LC-MS/MS analyses. Briefly, protein samples vary from several to hundreds of microliters are loaded onto the trap column at a high flow rate in short time at first, and after equilibrium the adsorbed peptides are eluted from the trap column to a reversed phase (RP) capillary column for LC separation. There are usually two types of instrument configurations for the automated sample injection in nanoflow LC-MS/MS analyses. In the first type of configuration, the trap column and RP separation capillary column are connected by a nanoflow Switching Valve. The flow through from trap column is directed to waste or LC separation column during sample injection or separation by the Switching Valve. Proteolytic digest without prior purification could be directly injected and cleaned up online in this case, which is important in proteome analysis due to the low sample loss. However, the void volume introduced by Switching Valve would seriously degrade the LC separation performance. The other one is vented column system in which trap and separation columns are directly connected via a microcross or microtee with an open/close Switching Valve. A regular 6-port Switching Valve could be used instead of using nanoflow Switching Valve in this type of configuration due to the mobile phase for LC separation does not pass though the Switching Valve. Further, to minimize the void volume introduced by the microcross or microtee, Licklider et al. packed the open space of microcross with C18 particles to reduce void volume. Meiring et al. drilled the microtee to 0.6 mm i.d. to fit a single micro sleeve having a V-shaped cut as a waste outlet, and the trap and analytical columns were butt-connected in this modified sleeve. However, these systems need tedious manual operation and the reproducibility is difficult to control.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis.
Journal of chromatography. A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (muHPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 microL on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis
Journal of Chromatography A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. © 2007 Elsevier B.V. All rights reserved.
Shun Feng - One of the best experts on this subject based on the ideXlab platform.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis.
Journal of chromatography. A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (muHPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 microL on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis
Journal of Chromatography A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. © 2007 Elsevier B.V. All rights reserved.
Ruijun Tian - One of the best experts on this subject based on the ideXlab platform.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis.
Journal of chromatography. A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (muHPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 microL on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis
Journal of Chromatography A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. © 2007 Elsevier B.V. All rights reserved.
Hongwei Liu - One of the best experts on this subject based on the ideXlab platform.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis.
Journal of chromatography. A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (muHPLC-MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5 microL on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis.
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Automated injection of uncleaned samples using a ten-port Switching Valve and a strong cation-exchange trap column for proteome analysis
Journal of Chromatography A, 2007Co-Authors: Fangjun Wang, Xiaogang Jiang, Shun Feng, Ruijun Tian, Xinning Jiang, Guanghui Han, Hongwei Liu, Hanfa ZouAbstract:Nanoliter flow rate is optimum for separation in capillary column liquid chromatography coupled with tandem mass spectrometry (HPLC–MS/MS). In order to develop a high-performance automated proteome analysis system allowing direct injection sample containing detergents, the influence of void volume varied from 0 to 5L on the separation performance and proteomic coverage of sample injection system using strong cation-exchange (SCX) trap column was investigated, it was found the void volume hardly affects the separation performance by using SCX trap column. Thus, a fully automated sample injection system using SCX trap column and ten-port Switching Valve was established for efficient shotgun proteome analysis. In this system, a nanoflow Switching Valve and a microtee were used to connect the SCX trap and analytical columns, and the uncleaned samples of proteolytic digests containing contaminants could be directly injected with minor influence on the separation performance, which was demonstrated to be a useful strategy in proteome analysis. © 2007 Elsevier B.V. All rights reserved.
