The Experts below are selected from a list of 288 Experts worldwide ranked by ideXlab platform
Walter Dewitte - One of the best experts on this subject based on the ideXlab platform.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
Development, 2018Co-Authors: Annika K Weimer, Juliana L Matos, Nidhi Sharma, Farah Patell, James A H Murray, Walter DewitteAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, 2-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose other cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1, is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
bioRxiv, 2017Co-Authors: Annika K Weimer, Juliana L Matos, James A H Murray, Walter Dewitte, Dominique C BergmannAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, two-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1 is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
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Stimulation of the final cell cycle in the stomatal lineage by the cyclin CYCD7;1 under regulation of the MYB transcription factor FOUR-LIPS
bioRxiv, 2017Co-Authors: Farah Patell-socha, James A H Murray, David Newman, Carl Collins, Erich Grotewold, Walter DewitteAbstract:Stomatal guard cells are formed through a sequence of aSymmetric and Symmetric Divisions in the epidermis of the sporophyte of most land plants. We show that several D-type cyclins are consecutively activated in the stomatal linage in the epidermis of Arabidopsis thaliana. Whereas CYCD2;1 and CYCD3;2 are activated in the meristemoids early in the lineage, CYCD7;1 is activated before the final Division. CYCD7;1 expression peaks in the guard mother cell, where its transcription is modulated by the FOUR-LIPS/MYB88 transcription factor. FOUR-LIPS/MYB88 interacts with the CYCD7;1 promoter and represses CYCD7;1 transcription. CYCD7;1 stimulates the final Symmetric Division in the stomatal lineage, since guard cell formation is delayed in the cycd7;1 mutant epidermis and guard mother cell (GMC) Divisions in four-lips mutant guard mother cells are limited by loss of function of CYCD7;1. Hence, the precise activation of a specific D-type cyclin, CYCD7;1, is required for correct timing of the last Symmetric Division that creates the stomatal guards cells, and CYCD7;1 expression is regulated by the FLP/MYB pathway that ensures cell cycle arrest in the stomatal guard cells.
Keiko U Torii - One of the best experts on this subject based on the ideXlab platform.
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mute directly orchestrates cell state switch and the single Symmetric Division to create stomata
Developmental Cell, 2018Co-Authors: Xingyun Qi, Kei Sugihara, Jonathan H Dang, Takaho A Endo, Kristen L Miller, Takashi Miura, Keiko U ToriiAbstract:Summary Precise cell Division control is critical for developmental patterning. For the differentiation of a functional stoma, a cellular valve for efficient gas exchange, the single Symmetric Division of an immediate precursor is absolutely essential. Yet, the mechanism governing this event remains unclear. Here we report comprehensive inventories of gene expression by the Arabidopsis bHLH protein MUTE, a potent inducer of stomatal differentiation. MUTE switches the gene expression program initiated by SPEECHLESS. MUTE directly induces a suite of cell-cycle genes, including CYCD5;1 , in which introduced expression triggers the Symmetric Divisions of arrested precursor cells in mute , and their transcriptional repressors, FAMA and FOUR LIPS . The regulatory network initiated by MUTE represents an incoherent type 1 feed-forward loop. Our mathematical modeling and experimental perturbations support a notion that MUTE orchestrates a transcriptional cascade leading to a tightly restricted pulse of cell-cycle gene expression, thereby ensuring the single cell Division to create functional stomata.
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mute directly orchestrates cell state switch and the single Symmetric Division to create stomata
bioRxiv, 2018Co-Authors: Xingyun Qi, Kei Sugihara, Jonathan H Dang, Takaho A Endo, Kristen L Miller, Takashi Miura, Keiko U ToriiAbstract:Precise cell Division control is critical for developmental patterning. For the differentiation of a functional stoma, a cellular valve for efficient gas exchange, the single Symmetric Division of an immediate precursor is absolutely essential. Yet, the mechanism governing the single Division event remains unclear. Here we report the complete inventories of gene expression by the Arabidopsis bHLH protein MUTE, a potent inducer of stomatal differentiation. MUTE switches the gene expression program initiated by its sister bHLH, SPEECHLESS. MUTE directly induces a suite of cell-cycle genes, including CYCD5;1, and their transcriptional repressors, FAMA and FOUR LIPS. The architecture of the regulatory network initiated by MUTE represents an Incoherent Type 1 Feed-Forward Loop. Our mathematical modeling and experimental perturbations support a notion that MUTE orchestrates a transcriptional cascade leading to the tightly-restricted, robust pulse of cell-cycle gene expression, thereby ensuring the single cell Division to create functional stomata.
Andrea H. Brand - One of the best experts on this subject based on the ideXlab platform.
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regulating the balance between Symmetric and aSymmetric stem cell Division in the developing brain
Fly, 2011Co-Authors: Boris Egger, Katrina S Gold, Andrea H. BrandAbstract:Stem cells proliferate through Symmetric Division or self-renew through aSymmetric Division whilst generating differentiating cell types. The balance between Symmetric and aSymmetric Division requires tight control to either expand a stem cell pool or to generate cell diversity. In the Drosophila optic lobe, Symmetrically dividing neuroepithelial cells transform into aSymmetrically dividing neuroblasts. The switch from neuroepithelial cells to neuroblasts is triggered by a proneural wave that sweeps across the neuroepithelium. Here we review recent findings showing that the orchestrated action of the Notch, EGFR, Fat-Hippo, and JAK/STAT signalling pathways controls the progression of the proneural wave and the sequential transition from Symmetric to aSymmetric Division. The neuroepithelial to neuroblast transition in the optic lobe bears many similarities to the switch from neuroepithelial cell to radial glial cell in the developing mammalian cerebral cortex. The Notch signalling pathway has a similar rol...
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Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe
Neural Development, 2007Co-Authors: Boris Egger, Jason Q. Boone, Naomi R Stevens, Andrea H. BrandAbstract:Background The choice of a stem cell to divide Symmetrically or aSymmetrically has profound consequences for development and disease. Unregulated Symmetric Division promotes tumor formation, whereas inappropriate aSymmetric Division affects organ morphogenesis. Despite its importance, little is known about how spindle positioning is regulated. In some tissues cell fate appears to dictate the type of cell Division, whereas in other tissues it is thought that stochastic variation in spindle position dictates subsequent sibling cell fate.
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Regulation of spindle orientation and neural stem cell fate in the Drosophila optic lobe
Neural Development, 2007Co-Authors: Boris Egger, Jason Q. Boone, Naomi R Stevens, Andrea H. BrandAbstract:Background The choice of a stem cell to divide Symmetrically or aSymmetrically has profound consequences for development and disease. Unregulated Symmetric Division promotes tumor formation, whereas inappropriate aSymmetric Division affects organ morphogenesis. Despite its importance, little is known about how spindle positioning is regulated. In some tissues cell fate appears to dictate the type of cell Division, whereas in other tissues it is thought that stochastic variation in spindle position dictates subsequent sibling cell fate. Results Here we investigate the relationship between neural progenitor identity and spindle positioning in the Drosophila optic lobe. We use molecular markers and live imaging to show that there are two populations of progenitors in the optic lobe: Symmetrically dividing neuroepithelial cells and aSymmetrically dividing neuroblasts. We use genetically marked single cell clones to show that neuroepithelial cells give rise to neuroblasts. To determine if a change in spindle orientation can trigger a neuroepithelial to neuroblast transition, we force neuroepithelial cells to divide along their apical/basal axis by misexpressing Inscuteable. We find that this does not induce neuroblasts, nor does it promote premature neuronal differentiation. Conclusion We show that Symmetrically dividing neuroepithelial cells give rise to aSymmetrically dividing neuroblasts in the optic lobe, and that regulation of spindle orientation and Division symmetry is a consequence of cell type specification, rather than a mechanism for generating cell type diversity.
James A H Murray - One of the best experts on this subject based on the ideXlab platform.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
Development, 2018Co-Authors: Annika K Weimer, Juliana L Matos, Nidhi Sharma, Farah Patell, James A H Murray, Walter DewitteAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, 2-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose other cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1, is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
bioRxiv, 2017Co-Authors: Annika K Weimer, Juliana L Matos, James A H Murray, Walter Dewitte, Dominique C BergmannAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, two-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1 is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
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Stimulation of the final cell cycle in the stomatal lineage by the cyclin CYCD7;1 under regulation of the MYB transcription factor FOUR-LIPS
bioRxiv, 2017Co-Authors: Farah Patell-socha, James A H Murray, David Newman, Carl Collins, Erich Grotewold, Walter DewitteAbstract:Stomatal guard cells are formed through a sequence of aSymmetric and Symmetric Divisions in the epidermis of the sporophyte of most land plants. We show that several D-type cyclins are consecutively activated in the stomatal linage in the epidermis of Arabidopsis thaliana. Whereas CYCD2;1 and CYCD3;2 are activated in the meristemoids early in the lineage, CYCD7;1 is activated before the final Division. CYCD7;1 expression peaks in the guard mother cell, where its transcription is modulated by the FOUR-LIPS/MYB88 transcription factor. FOUR-LIPS/MYB88 interacts with the CYCD7;1 promoter and represses CYCD7;1 transcription. CYCD7;1 stimulates the final Symmetric Division in the stomatal lineage, since guard cell formation is delayed in the cycd7;1 mutant epidermis and guard mother cell (GMC) Divisions in four-lips mutant guard mother cells are limited by loss of function of CYCD7;1. Hence, the precise activation of a specific D-type cyclin, CYCD7;1, is required for correct timing of the last Symmetric Division that creates the stomatal guards cells, and CYCD7;1 expression is regulated by the FLP/MYB pathway that ensures cell cycle arrest in the stomatal guard cells.
Juliana L Matos - One of the best experts on this subject based on the ideXlab platform.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
Development, 2018Co-Authors: Annika K Weimer, Juliana L Matos, Nidhi Sharma, Farah Patell, James A H Murray, Walter DewitteAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, 2-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose other cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1, is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
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lineage and stage specific expressed cycd7 1 coordinates the single Symmetric Division that creates stomatal guard cells
bioRxiv, 2017Co-Authors: Annika K Weimer, Juliana L Matos, James A H Murray, Walter Dewitte, Dominique C BergmannAbstract:Plants, with cells fixed in place by rigid walls, often utilize spatial and temporally distinct cell Division programs to organize and maintain organs. This leads to the question of how developmental regulators interact with the cell cycle machinery to link cell Division events with particular developmental trajectories. In Arabidopsis leaves, the development of stomata, two-celled epidermal valves that mediate plant-atmosphere gas exchange, relies on a series of oriented stem-cell-like aSymmetric Divisions followed by a single Symmetric Division. The stomatal lineage is embedded in a tissue whose cells transition from proliferation to post-mitotic differentiation earlier, necessitating stomatal lineage-specific factors to prolong competence to divide. We show that the D-type cyclin, CYCD7;1 is specifically expressed just prior to the Symmetric guard-cell forming Division, and that it is limiting for this Division. Further, we find that CYCD7;1 is capable of promoting Divisions in multiple contexts, likely through RBR-dependent promotion of the G1/S transition, but that CYCD7;1 is regulated at the transcriptional level by cell-type specific transcription factors that confine its expression to the appropriate developmental window.
