The Experts below are selected from a list of 2172 Experts worldwide ranked by ideXlab platform
Michele Bernasconi - One of the best experts on this subject based on the ideXlab platform.
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abstract lb 41 prolonged survival upon ultrasound enhanced doxorubicin delivery in two Syngenic glioblastoma mouse models
Cancer Research, 2014Co-Authors: Zsofia Kovaks, Beat Werner, Ernst Martinfiori, Michele BernasconiAbstract:Glioblastoma multiforme (GBM) is the most common and most aggressive malignant primary brain tumor, with a very poor prognosis. Chemotherapeutical treatment of GBM is limited by the blood-brain barrier (BBB). This physical and metabolic barrier separates the blood from the brain parenchyma and prevents the entry of toxins but also of potentially useful chemotherapeutics from the blood into the brain. Microbubble-enhanced focused ultrasound (MB-FUS) has been proposed to disrupt locally and reversibly the BBB to facilitate diffusion of drugs from the micro vasculature into brain tissue. The present study investigates the feasibility and the safety of such an approach in two Syngenic mouse models of GBM (GL261 and SMA-560). Local doxorubicin (DOX) concentration in MB-FUS sonicated normal brain tissue as well as in brain tumor tissue was increased as compared to the unsonicated control tissue in the contralateral hemisphere. Moreover, ultrasound mediated BBB disruption, in combination with DOX therapy, resulted in a significant increase of survival and in a slower disease progression in the two Syngenic GBM mouse models. In conclusion, our results confirm that MB-ultrasound might ultimately be an effective technology to improve the therapy of GBM, and they provide for the first time evidence that combining MB-FUS with DOX treatment is effective in Syngenic mouse models for GBM which can serve as preclinical models to study the impact of immune system on the therapeutic application of MB-FUS chemotherapy. Citation Format: Zsofia Kovaks, Beat Werner, Ernst Martin-Fiori, Michele Bernasconi. Prolonged survival upon ultrasound-enhanced doxorubicin delivery in two Syngenic glioblastoma mouse models. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr LB-41. doi:10.1158/1538-7445.AM2014-LB-41
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prolonged survival upon ultrasound enhanced doxorubicin delivery in two Syngenic glioblastoma mouse models
Journal of Controlled Release, 2014Co-Authors: Zsofia Kovacs, Beat Werner, Ernst Martinfiori, Anahita Rassi, Jorn Oliver Sass, Michele BernasconiAbstract:Glioblastoma multiforme (GBM) is the most common and most aggressive malignant primary brain tumor in humans with a very poor prognosis. Chemotherapeutical treatment of GBMs is limited by the blood-brain barrier (BBB). This physical and metabolic barrier separates the blood from the brain parenchyma and prevents the entry of toxins but also of potentially useful chemotherapeutics from the blood into the brain. Microbubble-enhanced focused ultrasound (MB-FUS) has been proposed to disrupt locally and reversibly the BBB to facilitate diffusion of drugs from the micro vasculature into brain tissue. The present study investigates the feasibility and the safety of such an approach in two Syngenic mouse models of GBM (GL261 and SMA-560). Local doxorubicin (DOX) concentration in MB-FUS sonicated normal brain tissue as well as in brain tumor tissue was increased as compared to the unsonicated control tissue in the contralateral hemisphere. Moreover, ultrasound mediated BBB disruption, in combination with DOX therapy, resulted in a significant increase of survival and in a slower disease progression in the two Syngenic GBM mouse models. In conclusion, our results confirm that MB-ultrasound might ultimately be an effective technology to improve the therapy of GBM, and they provide for the first time evidence that combining MB-FUS with DOX treatment is effective in Syngenic mouse models for GBM which can serve as preclinical models to study the impact of immune system on the therapeutic application of MB-FUS chemotherapy.
Hector N Seuanez - One of the best experts on this subject based on the ideXlab platform.
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evolutionary disruptions of human syntenic groups 3 12 14 and 15 in ateles paniscus chamek platyrrhini primates
Cytogenetic and Genome Research, 1999Co-Authors: Flavio Canavez, Miguel A. M. Moreira, Cibele R. Bonvicino, Peter Parham, Hector N SeuanezAbstract:Comparative gene assignments of 18 markers, based on analyses of somatic cell hybrids and previous data in the literature, indicated that human (HSA) syntenic groups 3, 12, 14, and 15 are dissociated in the spider monkey species Ateles paniscus chamek (APC). Markers present in HSA 3p were allocated to APC 3 and APC 9. The HSA 12 cluster was split into two syntenic groups, one mainly including HSA 12p markers in APC 16 and the other, including HSA 12q markers, in APC 2p. The HSA 14q cluster split into three syntenic groups, corresponding to APC 2q, APC 6, and APC 12. Finally, the HSA 15 cluster split into two syntenic groups, APC 2q and APC 3. Comparisons with previous gene assignments and human SROs led to the tentative postulation of rearrangements having occurred during the evolutionary divergence of man and A. paniscus chamek. Chromosome painting data in the congeneric species A. geoffroyi, other New World and Old World primates, and several representative non-primate animals were compared in an attempt to delineate the ancestral and derived conditions underlying the evolutionary rearrangement of syntenic groups in mammals.
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comparative gene assignment in ateles paniscus chamek platyrrhini primates and man association of three separate human syntenic groups and evolutionary considerations
Chromosoma, 1998Co-Authors: Flavio Canavez, Miguel A. M. Moreira, Cibele R. Bonvicino, Peter Parham, Hector N SeuanezAbstract:Regional assignment of eight markers to chromosome 2 of Ateles paniscus chamek (APC) confirmed a syntenic association similar to human (HSA) 12q + 14q + 15q. Three HSA 12q markers (RAP1B, PAH and ALDH2) were allocated to a shortest region of overlap (SRO) in APC 2p and found to be syntenic to other HSA 12q markers (PEPB and TCF1). Five HSA 14q markers (CTLA, PAX9, NSP, FOS and CHGA) were allocated to APC 2q and found to be syntenic to other HSA 14q markers (NP, TGM1, and CALM1) and to four HSA 15q markers (THBS1, B2M, HEXA and MPI) but dissociated from markers close to HSA 14qter (CKB) and HSA 15qter (FES-IDH2). Karyotypic comparisons showed an evident homoeology between APC 2p and HSA 12q while APC 2q was similar to an HSA 14qter::HSA 15qter fusion product. Comparative gene mapping data show that the HSA 14q + HSA 15q syntenic association is an ancestral mammalian gene cluster that has been maintained in several primate taxa. Conversely, in Ateles, it has been further associated with HSA 12q while, in Hominoids and Cebus, it has been independently dissociated into two separate syntenic groups, similar to HSA 14q and HSA 15q.
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Assignment of TCF1, TGM1, CALM1, CKB, THBS1, B2M, and FES in Ateles paniscus chamek (Platyrrhini, Primates).
Cytogenetics and cell genetics, 1997Co-Authors: Miguel A. M. Moreira, Flavio Canavez, Peter Parham, Hector N SeuanezAbstract:Regional assignment of five markers to chromosome 2 of Ateles paniscus chamek (APC) confirmed a syntenic association similar to human (HSA) 12q + 14q + 15q. TCF1 was allocated to a shortest region of overlap (SRO) in APC 2p and found to be syntenic to PEPB, while TGM1, CALM1, THBS1, and B2M were assigned to APC 2q, being syntenic to NP, HEXA, and MPI. Conversely, markers close to HSA 14qter (CKB) and HSA 15qter (FES-IDH2) were relocated to other Ateles syntenic groups. Karyotypic comparisons showed an evident homoeology between APC 2p and HSA 12q, whereas APC 2q was similar to an HSA 14qter::HSA 15qter fusion product.
Sung Hwa Hong - One of the best experts on this subject based on the ideXlab platform.
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characteristics of mouse adipose tissue derived stem cells and therapeutic comparisons between syngeneic and allogeneic adipose tissue derived stem cell transplantation in experimental autoimmune thyroiditis
Cell Transplantation, 2014Co-Authors: Eun Wha Choi, Il Seob Shin, So Young Park, Eun Ji Yoon, Sung Keun Kang, Sung Hwa HongAbstract:Previously, we found that the intravenous administration of human adipose tissue-derived mesenchymal stem cells was a promising therapeutic option for autoimmune thyroiditis even when the cells were transplanted into a xenogeneic model without an immunosuppressant. Therefore, we explored the comparison between the therapeutic effects of syngeneic and allogeneic adipose tissue-derived stem cells on an experimental autoimmune thyroiditis mouse model. Experimental autoimmune thyroiditis was induced in C57BL/6 mice by immunization with porcine thyroglobulin. Adipose tissue-derived stem cells derived from C57BL/6 mice (syngeneic) or BALB/c mice (allogeneic) or saline as a vehicle control were administered intravenously four times weekly. Blood and tissue samples were collected 1 week after the last transplantation. Adipose tissue-derived stem cells from mice were able to differentiate into multiple lineages in vitro; however, mouse adipose tissue-derived stem cells did not have immunophenotypes identical to those from humans. Syngeneic and allogeneic administrations of adipose tissue-derived stem cells reduced thyroglobulin autoantibodies and the inflammatory immune response, protected against lymphocyte infiltration into the thyroid, and restored the Th1/Th2 balance without any adverse effects. However, different humoral immune responses were observed for infused cells from different stem cell sources. The strongest humoral immune response was induced by xenogeneic transplantation, followed by allogeneic and syngeneic administration, in that order. The stem cells were mostly found in the spleen, not the thyroid. This migration might be because the stem cells primarily function in systemic immune modulation, due to being given prior to disease induction. In this study, we confirmed that there were equal effects of adipose tissue-derived stem cells in treating autoimmune thyroiditis between syngeneic and allogeneic transplantations.
Dou Kefeng - One of the best experts on this subject based on the ideXlab platform.
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prolongation of skin allograft survival in mice by Syngenic hematopoietic stem cell transplantation
Current Immunology, 2007Co-Authors: Wang Lin, Zhou Jingshi, Zhao Qingchuan, Han Hua, Yi Wei, Wang Yaochun, Dou KefengAbstract:To investigate the mechanism of tolerance of organ transplantation following Syngenic hematopoietic stem cell transplantation(HSCT),the mouse model was constructed by allogenic skin transplantation,and FK506 was injected into peritoneal cavity 2 weeks later.Three weeks later,the treated group was exposed to total body irradiation and accepted the Syngenic bone marrow transplantation from GFP C57BL/6 transgenic mice.The viability of chimeric mice and graft were observed constantly.The GFP expression in peripheral blood was analyzed by FACS;the specification of induced tolerance was detected by MLR.It was found that the survival time of mouse from treated group was(29.14±4.92) d,significantly longer than the control group(P0.05).The expression of GFP following BMT was 82%(4w) and 91%(6w) respectively.The outcome of MLR and DTH for the treated group had significant difference to the control,P0.05.It is concluded that Syngenic HSCT combined with immunosuppressive treatment may induce tolerance to skin transplantation.
Flavio Canavez - One of the best experts on this subject based on the ideXlab platform.
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evolutionary disruptions of human syntenic groups 3 12 14 and 15 in ateles paniscus chamek platyrrhini primates
Cytogenetic and Genome Research, 1999Co-Authors: Flavio Canavez, Miguel A. M. Moreira, Cibele R. Bonvicino, Peter Parham, Hector N SeuanezAbstract:Comparative gene assignments of 18 markers, based on analyses of somatic cell hybrids and previous data in the literature, indicated that human (HSA) syntenic groups 3, 12, 14, and 15 are dissociated in the spider monkey species Ateles paniscus chamek (APC). Markers present in HSA 3p were allocated to APC 3 and APC 9. The HSA 12 cluster was split into two syntenic groups, one mainly including HSA 12p markers in APC 16 and the other, including HSA 12q markers, in APC 2p. The HSA 14q cluster split into three syntenic groups, corresponding to APC 2q, APC 6, and APC 12. Finally, the HSA 15 cluster split into two syntenic groups, APC 2q and APC 3. Comparisons with previous gene assignments and human SROs led to the tentative postulation of rearrangements having occurred during the evolutionary divergence of man and A. paniscus chamek. Chromosome painting data in the congeneric species A. geoffroyi, other New World and Old World primates, and several representative non-primate animals were compared in an attempt to delineate the ancestral and derived conditions underlying the evolutionary rearrangement of syntenic groups in mammals.
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comparative gene assignment in ateles paniscus chamek platyrrhini primates and man association of three separate human syntenic groups and evolutionary considerations
Chromosoma, 1998Co-Authors: Flavio Canavez, Miguel A. M. Moreira, Cibele R. Bonvicino, Peter Parham, Hector N SeuanezAbstract:Regional assignment of eight markers to chromosome 2 of Ateles paniscus chamek (APC) confirmed a syntenic association similar to human (HSA) 12q + 14q + 15q. Three HSA 12q markers (RAP1B, PAH and ALDH2) were allocated to a shortest region of overlap (SRO) in APC 2p and found to be syntenic to other HSA 12q markers (PEPB and TCF1). Five HSA 14q markers (CTLA, PAX9, NSP, FOS and CHGA) were allocated to APC 2q and found to be syntenic to other HSA 14q markers (NP, TGM1, and CALM1) and to four HSA 15q markers (THBS1, B2M, HEXA and MPI) but dissociated from markers close to HSA 14qter (CKB) and HSA 15qter (FES-IDH2). Karyotypic comparisons showed an evident homoeology between APC 2p and HSA 12q while APC 2q was similar to an HSA 14qter::HSA 15qter fusion product. Comparative gene mapping data show that the HSA 14q + HSA 15q syntenic association is an ancestral mammalian gene cluster that has been maintained in several primate taxa. Conversely, in Ateles, it has been further associated with HSA 12q while, in Hominoids and Cebus, it has been independently dissociated into two separate syntenic groups, similar to HSA 14q and HSA 15q.
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Assignment of TCF1, TGM1, CALM1, CKB, THBS1, B2M, and FES in Ateles paniscus chamek (Platyrrhini, Primates).
Cytogenetics and cell genetics, 1997Co-Authors: Miguel A. M. Moreira, Flavio Canavez, Peter Parham, Hector N SeuanezAbstract:Regional assignment of five markers to chromosome 2 of Ateles paniscus chamek (APC) confirmed a syntenic association similar to human (HSA) 12q + 14q + 15q. TCF1 was allocated to a shortest region of overlap (SRO) in APC 2p and found to be syntenic to PEPB, while TGM1, CALM1, THBS1, and B2M were assigned to APC 2q, being syntenic to NP, HEXA, and MPI. Conversely, markers close to HSA 14qter (CKB) and HSA 15qter (FES-IDH2) were relocated to other Ateles syntenic groups. Karyotypic comparisons showed an evident homoeology between APC 2p and HSA 12q, whereas APC 2q was similar to an HSA 14qter::HSA 15qter fusion product.
