The Experts below are selected from a list of 27 Experts worldwide ranked by ideXlab platform
A Struglics - One of the best experts on this subject based on the ideXlab platform.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Introduction Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays. Synovial Fluid (SF) from 26 people with healthy knees (reference) and 269 patients were analyzed in a cross-sectional study. Patient groups were acute inflammatory arthritis, acute knee injury, chronic knee injury and knee osteoarthritis (OA). Aggrecan ARGS fragments were assayed by ELISA using the monoclonal antibody OA-1. Total aggrecan content was analyzed by an ELISA using the monoclonal antibody 1-F21, and sulfated glycosaminoglycan by Alcian blue precipitation. Aggrecan ARGS fragment concentrations in all groups differed from the reference group (P < 0.001). The acute inflammatory arthritis group had the highest median Level, 177-fold greater than that of the reference group. Median Levels (in pmol ARGS/ml SF) were: reference 0.5, acute inflammatory arthritis 88.5, acute knee injury 53.9, chronic knee injury 0.5 and OA 4.6. In contrast, aggrecan and sulfated glycosaminoglycan concentrations varied much less between groups, and only acute inflammatory arthritis and acute knee injury were found to have a two-fold increase in median Levels compared to the reference. Levels of aggrecan ARGS fragments in human Synovial Fluid are increased in human arthritis, OA and after knee injury, likely reflecting an enhanced cleavage at the 392Glu-393Ala bond in the IGD by aggrecanase. An assay that specifically quantified these fragments better distinguished samples from joints with pathology than assays monitoring aggrecan or glycosaminoglycan concentrations. The newly developed ARGS fragment assay can be used to monitor aggrecanase activity in human joint disease and experimental models.
S Larsson - One of the best experts on this subject based on the ideXlab platform.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Introduction Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays. Synovial Fluid (SF) from 26 people with healthy knees (reference) and 269 patients were analyzed in a cross-sectional study. Patient groups were acute inflammatory arthritis, acute knee injury, chronic knee injury and knee osteoarthritis (OA). Aggrecan ARGS fragments were assayed by ELISA using the monoclonal antibody OA-1. Total aggrecan content was analyzed by an ELISA using the monoclonal antibody 1-F21, and sulfated glycosaminoglycan by Alcian blue precipitation. Aggrecan ARGS fragment concentrations in all groups differed from the reference group (P < 0.001). The acute inflammatory arthritis group had the highest median Level, 177-fold greater than that of the reference group. Median Levels (in pmol ARGS/ml SF) were: reference 0.5, acute inflammatory arthritis 88.5, acute knee injury 53.9, chronic knee injury 0.5 and OA 4.6. In contrast, aggrecan and sulfated glycosaminoglycan concentrations varied much less between groups, and only acute inflammatory arthritis and acute knee injury were found to have a two-fold increase in median Levels compared to the reference. Levels of aggrecan ARGS fragments in human Synovial Fluid are increased in human arthritis, OA and after knee injury, likely reflecting an enhanced cleavage at the 392Glu-393Ala bond in the IGD by aggrecanase. An assay that specifically quantified these fragments better distinguished samples from joints with pathology than assays monitoring aggrecan or glycosaminoglycan concentrations. The newly developed ARGS fragment assay can be used to monitor aggrecanase activity in human joint disease and experimental models.
Stefan L Lohmander - One of the best experts on this subject based on the ideXlab platform.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Introduction Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays.
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Synovial Fluid Level of aggrecan args fragments is a more sensitive marker of joint disease than glycosaminoglycan or aggrecan Levels a cross sectional study
Arthritis Research & Therapy, 2009Co-Authors: S Larsson, Stefan L Lohmander, A StruglicsAbstract:Aggrecanase cleavage at the 392Glu-393Ala bond in the interglobular domain (IGD) of aggrecan, releasing N-terminal 393ARGS fragments, is an early key event in arthritis and joint injuries. Here, we use a quantitative immunoassay of aggrecan ARGS neoepitope fragments in human Synovial Fluid to determine if this cleavage-site specific method better identifies joint pathology than previously available less specific aggrecan assays. Synovial Fluid (SF) from 26 people with healthy knees (reference) and 269 patients were analyzed in a cross-sectional study. Patient groups were acute inflammatory arthritis, acute knee injury, chronic knee injury and knee osteoarthritis (OA). Aggrecan ARGS fragments were assayed by ELISA using the monoclonal antibody OA-1. Total aggrecan content was analyzed by an ELISA using the monoclonal antibody 1-F21, and sulfated glycosaminoglycan by Alcian blue precipitation. Aggrecan ARGS fragment concentrations in all groups differed from the reference group (P < 0.001). The acute inflammatory arthritis group had the highest median Level, 177-fold greater than that of the reference group. Median Levels (in pmol ARGS/ml SF) were: reference 0.5, acute inflammatory arthritis 88.5, acute knee injury 53.9, chronic knee injury 0.5 and OA 4.6. In contrast, aggrecan and sulfated glycosaminoglycan concentrations varied much less between groups, and only acute inflammatory arthritis and acute knee injury were found to have a two-fold increase in median Levels compared to the reference. Levels of aggrecan ARGS fragments in human Synovial Fluid are increased in human arthritis, OA and after knee injury, likely reflecting an enhanced cleavage at the 392Glu-393Ala bond in the IGD by aggrecanase. An assay that specifically quantified these fragments better distinguished samples from joints with pathology than assays monitoring aggrecan or glycosaminoglycan concentrations. The newly developed ARGS fragment assay can be used to monitor aggrecanase activity in human joint disease and experimental models.
Xianlong Zhang - One of the best experts on this subject based on the ideXlab platform.
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soluble p selectin in Synovial Fluid Level is correlated with the radiographic severity of knee osteoarthritis
Clinica Chimica Acta, 2010Co-Authors: Tao Cheng, Song Zhao, Xiaochun Peng, Xianlong ZhangAbstract:Abstract Background Recent studies provide evidence that inflammation is a feature of the disease process in Osteoarthritis (OA). The clinical significance of P selectin (Ps) in OA has not been adequately studied and the association between Ps Level and OA severity remains unknown. Methods We enrolled 120 knee OA subjects and 45 controls. All patients were scored for Kellgren–Lawrence grade (0–4). The Ps in serum and Synovial Fluid (SF) as well as serum C-reactive protein (CRP) Levels were detected. Results The mean Ps Level in OA subjects was markedly increased than that in controls. In OA patients, the SF Ps Levels increased with the severity of KL scores and significantly correlated with severity of disease (r = 0.546, P Conclusion The Ps Levels in SF were significantly correlated with the severity of OA, suggesting that it may be used as a biomarker to evaluate the progression of OA.
K Shimizu - One of the best experts on this subject based on the ideXlab platform.
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procollagen ii c propeptide Level in the Synovial Fluid as a predictor of radiographic progression in early knee osteoarthritis
Annals of the Rheumatic Diseases, 2003Co-Authors: S Sugiyama, M Itokazu, Y Suzuki, K ShimizuAbstract:Objective: To investigate the prognostic value of procollagen type II carboxy-terminal propeptide (PIICP) Level in Synovial Fluid in relation to early tibiofemoral joint osteoarthritis (OA). Methods: Data were collected on 172 women (age 40 to 59 years) who had knee pain and tibiofemoral joint OA in the early stage. Standing semiflexed knee radiographs were obtained by fluoroscopy at baseline and at four year follow up and a computerised, magnification corrected measurement system was applied to measurement of minimal joint space width in the tibiofemoral compartment. Synovial Fluid sampling was performed at baseline and at the four year follow up. Levels of PIICP in the Synovial Fluid were measured by enzyme immunoassay. The outcome measures were assessed by radiographic joint space narrowing (JSN) in the tibiofemoral joints over four years. Multiple linear regression analyses were used to examine the relation between radiographic JSN and Synovial Fluid Level of PIICP. Results: The number of women available at both baseline and at four year follow up was 110. The average of radiographic JSN over four years was 0.53 mm (range 0.00-2.01). Body mass index showed a slightly positive association with baseline PIICP Level. In multiple linear regression analyses adjusted for age and body mass index, radiographic JSN over four years had a direct positive correlation with baseline PIICP Level (r=0.395; 95% confidence interval (95% CI) 0.231 to 0.529; p<0.001). Conclusion: In a four year prospective study of women, quantification of Synovial Fluid PIICP was able to predict subsequent radiographic progression in early tibiofemoral joint OA.
