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Marc E Levenston - One of the best experts on this subject based on the ideXlab platform.
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osmotic swelling responses are conserved across cartilaginous tissues with varied Sulfated Glycosaminoglycan contents
Journal of Orthopaedic Research, 2020Co-Authors: Eva Gabriela Baylon, Marc E LevenstonAbstract:Determining the influence of tissue composition on the osmotic swelling stress of articular cartilage and meniscus fibrocartilage is important to enhance our understanding of physiology and disease. This osmotic swelling stress is critical for the load-bearing capability of both tissues and results in part due to the interactions between the negatively charged Sulfated Glycosaminoglycan (sGAG) chains and the ionic interstitial fluid. Changes in sGAG content, as those occurring during the progression of degenerative joint disease, alter such interactions. Here, we compare the time-varying effects of altered osmotic environments on the confined compression swelling behavior of bovine tissues spanning a range of sGAG concentrations: juvenile articular cartilage, juvenile and adult meniscus, and juvenile cartilage enzymatically degraded to reduce its sGAG content. The transient response to changes in bath conditions was evaluated for explants assigned to one of three compressive offsets (5%, 10%, or 15% strain) and one of three bath conditions (0.1X, 1X, or 10X phosphate-buffered saline). Our results show that relative responses to alterations to the osmotic environment are consistent across native tissues but differ for degraded juvenile cartilage, demonstrating that changes in sGAG do not completely recapitulate the native swelling behaviors. Further, we found a strong correlation between aggregate modulus and sGAG/collagen, as well as between sGAG and collagen contents across native tissue types, suggesting some conservation of composition-function relationships across a range of tissue types with varying sGAG concentrations. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:785-792, 2020.
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osmotic swelling responses are conserved across cartilaginous tissues with varied Sulfated Glycosaminoglycan contents
bioRxiv, 2018Co-Authors: Eva Gabriela Baylon, Marc E LevenstonAbstract:The interactions between the negatively charged Sulfated Glycosaminoglycan (sGAG) chains and the ionic interstitial fluid in articular cartilage and meniscal fibrocartilage give rise to an osmotic swelling stress that is critical for the load-bearing capability of both tissues. This osmotic swelling stress is altered when the sGAG content is changed, as during progression of degenerative joint disease; understanding the influence of sGAG concentration on the osmotic swelling stress of cartilage and meniscus is important to enhance our understanding of physiology and disease. This study compared the effect of altered osmotic environments on the confined compression swelling behavior of bovine tissues spanning a range of sGAG concentrations: juvenile articular cartilage, juvenile and adult meniscus, and juvenile cartilage degraded to reduce sGAG content. The transient response to changes in bath conditions was evaluated for explants assigned to one of three compressive offsets (5%, 10%, or 15% strain) and one of three bath conditions (0.1X, 1X, or 10X Phosphate Buffered Saline). Our results show that relative responses to alterations to the osmotic environment are consistent across tissue types, demonstrating that the role of sGAG in the swelling properties of the tissues tested is conserved, even when sGAG is present at low concentrations. Additionally, this study found unexpected correlations across tissue types between sGAG and collagen contents and between the aggregate modulus and both sGAG and collagen contents. These results suggest some conservation of composition-function relationships across a range of tissue types.
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fact versus artifact avoiding erroneous estimates of Sulfated Glycosaminoglycan content using the dimethylmethylene blue colorimetric assay for tissue engineered constructs
European Cells & Materials, 2015Co-Authors: C H Zheng, Marc E LevenstonAbstract:The 1,9-dimethylmethylene blue (DMMB) assay is widely used to quantify Sulfated Glycosaminoglycan (sGAG) contents of engineered tissues, culture media, tissue samples and bodily fluids, but is subject to interference from polyanions such as hyaluronic acid (HA), DNA and RNA. We examined whether specific combinations of dye pH and absorbance wavelength could minimize non-sGAG artifacts without compromising DMMB assay sensitivity. HA and DNA solutions generated substantial signal at pH 3 but not at pH 1.5. Reducing dye pH did not significantly alter sGAG measurements for normal cartilage and meniscus tissues, but eliminated anomalously high apparent sGAG contents for enzymatically isolated chondrocytes, adipose-derived stem cell (ADSC)-agarose constructs and ADSC pellets. In a cartilage tissue engineering case study, pH 3 dye indicated high apparent sGAG readings throughout culture in both basal and chondrogenic media, with a marked decline between day 14 and 21 for chondrogenic constructs. The pH 1.5 dye, however, indicated minimal sGAG accumulation in basal medium and stable sGAG content throughout culture in chondrogenic medium. As it is often difficult to know a priori whether all groups in a study will have sGAG contents high enough to overwhelm artifacts, we recommend modifying the standard DMMB assay to reduce the risk of spurious findings in tissue engineering and clinical research. Specifically, we recommend shifting to a pH 1.5 DMMB dye and basing quantification on the absorbance difference between 525 nm (μ peak) and 595 nm (β peak) to compensate for the moderate loss of sensitivity associated with reducing the dye pH.
Piyaratana Tosukhowong - One of the best experts on this subject based on the ideXlab platform.
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urinary Sulfated Glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis
PLOS ONE, 2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Monpicha Srisaart, Piyaratana TosukhowongAbstract:Familial members of urolithiasis have high risk for stone development. We observed the low Sulfated Glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of Sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary Sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary Sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per Sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.
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Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Monpicha Srisa-art, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Piyaratana TosukhowongAbstract:Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
Thasinas Dissayabutra - One of the best experts on this subject based on the ideXlab platform.
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urinary Sulfated Glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis
PLOS ONE, 2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Monpicha Srisaart, Piyaratana TosukhowongAbstract:Familial members of urolithiasis have high risk for stone development. We observed the low Sulfated Glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of Sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary Sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary Sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per Sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.
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Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Monpicha Srisa-art, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Piyaratana TosukhowongAbstract:Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
Eva Gabriela Baylon - One of the best experts on this subject based on the ideXlab platform.
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osmotic swelling responses are conserved across cartilaginous tissues with varied Sulfated Glycosaminoglycan contents
Journal of Orthopaedic Research, 2020Co-Authors: Eva Gabriela Baylon, Marc E LevenstonAbstract:Determining the influence of tissue composition on the osmotic swelling stress of articular cartilage and meniscus fibrocartilage is important to enhance our understanding of physiology and disease. This osmotic swelling stress is critical for the load-bearing capability of both tissues and results in part due to the interactions between the negatively charged Sulfated Glycosaminoglycan (sGAG) chains and the ionic interstitial fluid. Changes in sGAG content, as those occurring during the progression of degenerative joint disease, alter such interactions. Here, we compare the time-varying effects of altered osmotic environments on the confined compression swelling behavior of bovine tissues spanning a range of sGAG concentrations: juvenile articular cartilage, juvenile and adult meniscus, and juvenile cartilage enzymatically degraded to reduce its sGAG content. The transient response to changes in bath conditions was evaluated for explants assigned to one of three compressive offsets (5%, 10%, or 15% strain) and one of three bath conditions (0.1X, 1X, or 10X phosphate-buffered saline). Our results show that relative responses to alterations to the osmotic environment are consistent across native tissues but differ for degraded juvenile cartilage, demonstrating that changes in sGAG do not completely recapitulate the native swelling behaviors. Further, we found a strong correlation between aggregate modulus and sGAG/collagen, as well as between sGAG and collagen contents across native tissue types, suggesting some conservation of composition-function relationships across a range of tissue types with varying sGAG concentrations. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 38:785-792, 2020.
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osmotic swelling responses are conserved across cartilaginous tissues with varied Sulfated Glycosaminoglycan contents
bioRxiv, 2018Co-Authors: Eva Gabriela Baylon, Marc E LevenstonAbstract:The interactions between the negatively charged Sulfated Glycosaminoglycan (sGAG) chains and the ionic interstitial fluid in articular cartilage and meniscal fibrocartilage give rise to an osmotic swelling stress that is critical for the load-bearing capability of both tissues. This osmotic swelling stress is altered when the sGAG content is changed, as during progression of degenerative joint disease; understanding the influence of sGAG concentration on the osmotic swelling stress of cartilage and meniscus is important to enhance our understanding of physiology and disease. This study compared the effect of altered osmotic environments on the confined compression swelling behavior of bovine tissues spanning a range of sGAG concentrations: juvenile articular cartilage, juvenile and adult meniscus, and juvenile cartilage degraded to reduce sGAG content. The transient response to changes in bath conditions was evaluated for explants assigned to one of three compressive offsets (5%, 10%, or 15% strain) and one of three bath conditions (0.1X, 1X, or 10X Phosphate Buffered Saline). Our results show that relative responses to alterations to the osmotic environment are consistent across tissue types, demonstrating that the role of sGAG in the swelling properties of the tissues tested is conserved, even when sGAG is present at low concentrations. Additionally, this study found unexpected correlations across tissue types between sGAG and collagen contents and between the aggregate modulus and both sGAG and collagen contents. These results suggest some conservation of composition-function relationships across a range of tissue types.
Kanokporn Chindaphan - One of the best experts on this subject based on the ideXlab platform.
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urinary Sulfated Glycosaminoglycan insufficiency and chondroitin sulfate supplement in urolithiasis
PLOS ONE, 2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Monpicha Srisaart, Piyaratana TosukhowongAbstract:Familial members of urolithiasis have high risk for stone development. We observed the low Sulfated Glycosaminoglycan (GAG) excretion in urolithiasis patients and their descendants. In this study, we investigated urinary excretion of Sulfated GAG, chondroitin sulfate (CS), heparan sulfate (HS) and hyaluronic acid (HA) in urolithiasis and their children, and explored the effect of CS and HA supplement in urolithic hyperoxaluric rats. The 24-hour urines were collected from urolithiasis patients (28) and their children (40), as well as healthy controls (45) and their children (33) to measure urinary Sulfated GAG, CS, HS and HA excretion rate. Our result showed that urinary Sulfated GAG and CS were diminished in both urolithiasis patients and their children, while decreased HS and increased HA were observed only in urolithiasis patients. Percentage of HS per Sulfated GAG increased in both urolithiasis patients and their children. In hyperoxaluric rats induced by ethylene glycol and vitamin D, we found that CS supplement could prevent stone formation, while HA supplement had no effect on stone formation. Our study revealed that decreased urinary GAG and CS excretion are common in familial members of urolithiasis patients, and CS supplement might be beneficial in calcium oxalate urolithiasis prophylaxis for hyperoxaluric patients.
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Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
2019Co-Authors: Thasinas Dissayabutra, Nuttiya Kalpongnukul, Kanokporn Chindaphan, Monpicha Srisa-art, Wattanachai Ungjaroenwathana, Maroot Kaewwongse, Kroonpong Iampenkhae, Piyaratana TosukhowongAbstract:Chondroitin sulfate and heparan sulfate fraction in total Sulfated Glycosaminoglycan in urine of healthy controls, urolithiasis patients, children of control (CC) and children of urolithiasis (UC).
