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Hong-kean Ooi - One of the best experts on this subject based on the ideXlab platform.
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Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification.
Veterinary parasitology, 2008Co-Authors: Joan Dee C Parel, Jedhan U Galula, Hong-kean OoiAbstract:To differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata and Syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 (ITS-1), 5.8S gene, and ITS-2 of the ribosomal DNA followed by designing of species-specific primers for future use in the identification of the worms. It was observed that S. obvelata, S. muris and Aspiculuris tetraptera can be differentiated from each other based on their rDNA sequences. This is the first report of the ITS-1, 5.8S, and ITS-2 of the rDNA of the three aforementioned rodent pinworm species. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species. Moreover, we also constructed species-specific primers that were designed for unique regions of the ITS-2 of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogenous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice, and wild rodents.
Julia Maria Costacruz - One of the best experts on this subject based on the ideXlab platform.
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reducao da carga parasitaria de strogyloides venezuelensis pela coinfeccao com Syphacia muris em ratos rattus norvegicus wistar
INVESTIGAÇÃO, 2016Co-Authors: Jose Eduardo Neto De Sousa, Edson Fernando Goulart De Carvalho, Marcelo Arantes Levenhagen, Lucas Silva De Faria, Julia Maria CostacruzAbstract:O nematodeo Syphacia muris infecta naturalmente ratos sendo um problema em bioterio convencionais. A especie Strongyloides venezuelensis e um parasito de roedores utilizado no estudo da relacao parasito/hospedeiro devido a semelhanca do ciclo biologico de Strongyloides stercoralis . O presente estudo teve como objetivo avaliar a carga parasitaria entre ratos ( Rattus norvegicus ) Wistar naturalmente infectados com S. muris e experimentalmente infectados com S. venezuelensis . Foram utilizados 40 animais divididos em quatro grupos de 10 animais: Grupo I ratos sem infeccao, Grupo II ratos infectados com S. venezuelensis , Grupo III ratos infectados com S. muris , Grupo IV ratos coinfectados com S. muris e S. venezuelensis . A carga parasitaria foi avaliada por meio da quantificacao dos helmintos recuperados dos intestinos dos animais no 21o dia apos infeccao (d.p.i) e tambem da contagem de ovos por gramas de fezes (OPG) diariamente ate 21 d.p.i. Para analise estatistica foi utilizando o teste de Mann Whitney U seguido pelo pos-teste de Bonferroni e tambem utilizado o programa eggCounts R para determinar a porcentagem de reducao de ovos. Os parasitos adultos recuperados do intestino dos ratos dos Grupos II e III foram maiores comparados com o grupo IV (p<0,0001). A eliminacao de ovos de S. venezuelensis dos grupos II e IV comecou no 5 d.p.i. e teve o pico de eliminacao no 8 d.p.i. No entanto, o Grupo II eliminou mais ovos (p<0,0001) quando comparado com o grupo Grupo IV. Ao analisar os dois grupos com os pacotes eggCounts R, em 5-9, 14, 15 e 19 dpi, houve uma reducao de mais de 50% na eliminacao de ovos, enquanto que 16, 18, 20 e 21 dpi, teve reducao menor que 50%. Foi demonstrado que a eliminacao de ovos bem como a quantidade de femeas partenogeneticas de S. venezuelensis diminui significativamente pela coinfeccao por S. muris .
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serological cross reactivity between strongyloides venezuelensis and Syphacia muris in wistar rats rattus norvegicus
Parasitology International, 2016Co-Authors: Jose Eduardo Neto De Sousa, Edson Fernando Goulart De Carvalho, Marcelo Arantes Levenhagen, Lucas Silva De Faria, Maria Do Rosario De Fatima Goncalvespires, Julia Maria CostacruzAbstract:One of the problems frequently faced in laboratory facilities is the possibility of the natural parasitic infection of lab animals, which can interfere with biomedical research results. The present study aimed to evaluate cross-reactivity among serum samples from Wistar rats (Rattus norvegicus) naturally infected with Syphacia muris and experimentally infected with Strongyloides venezuelensis. Forty rats were divided into four groups of ten animals each. Parasite load was evaluated by quantifying the adult worms from both helminthes species recovered from the intestines and the S. venezuelensis eggs eliminated in feces. Serological cross-reactivity by parasite-specific IgG detection was tested via enzyme linked immunosorbent assay (ELISA), immunofluorescence antibody test (IFAT) and immunoblotting. The results demonstrated that the quantity of S. venezuelensis eliminated eggs and parthenogenetic females decreased significantly in cases of co-infection with S. muris. ELISA revealed 100% cross-reactivity of serum samples from both species against the opposing antigen. IgG cross-reactivity was confirmed by IFAT using tissue sections of S. venezuelensis larvae and adult S. muris. Immunoblotting showed that IgG antibodies from the sera of animals infected with S. muris recognized eight antigenic bands from S. venezuelensis saline extract and that IgG antibodies from the sera of animals infected with S. venezuelensis recognized seven bands from S. muris saline extract. These results demonstrate the serological cross-reactivity between S. muris and S. venezuelensis in infected rats.
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diagnosis of the pinworm Syphacia muris in the wistar rat rattus norvegicus
Journal of Helminthology, 2016Co-Authors: Jose Eduardo Neto De Sousa, Edson Fernando Goulart De Carvalho, Marcelo Arantes Levenhagen, L A Chaves, Julia Maria CostacruzAbstract:This study aimed to compare three qualitative parasitological methods for the diagnosis of Syphacia muris infection in 30 Wistar rats (Rattus norvegicus) infected naturally. Methods of spontaneous sedimentation (Hoffman, Pons and Janer, or HPJ) and spontaneous flotation (Willis) for faecal samples and a method of taping (Graham) were performed and compared. The Graham and Willis methods were more sensitive than the HPJ method (P< 0.05). The Graham method was able to detect S. muris eggs in 100% of the samples. Eggs were detected in 83% and 60% of the samples using the Willis and HPJ methods, respectively. Method choice is important for screening for parasites of rats kept under laboratory conditions, as accurate diagnosis helps prevent future environmental contamination and infection. We concluded that the Graham method was the most efficient of those tested in this study for detection of S. muris infection in rats. This method is also rapid, inexpensive and practical, and should be implemented as a necessary measure for infection control.
Joan Dee C Parel - One of the best experts on this subject based on the ideXlab platform.
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Characterization of rDNA sequences from Syphacia obvelata, Syphacia muris, and Aspiculuris tetraptera and development of a PCR-based method for identification.
Veterinary parasitology, 2008Co-Authors: Joan Dee C Parel, Jedhan U Galula, Hong-kean OoiAbstract:To differentiate the morphologically similar pinworms of the common laboratory rodents, such as Syphacia obvelata and Syphacia muris, we amplified and sequenced the region spanning the internal transcribed spacer 1 (ITS-1), 5.8S gene, and ITS-2 of the ribosomal DNA followed by designing of species-specific primers for future use in the identification of the worms. It was observed that S. obvelata, S. muris and Aspiculuris tetraptera can be differentiated from each other based on their rDNA sequences. This is the first report of the ITS-1, 5.8S, and ITS-2 of the rDNA of the three aforementioned rodent pinworm species. The use of restriction endonucleases, AluI or RsaI, further allowed the delineation of the three species. Moreover, we also constructed species-specific primers that were designed for unique regions of the ITS-2 of the three species. This approach allowed their specific identification with no amplicons being amplified from heterogenous DNA samples, and sequencing confirmed the identity of the sequences amplified. Thus, the use of these specific primers along with PCR-RFLP can serve as useful tools for the identification of pinworms in rats, mice, and wild rodents.
Aziz Bulbul - One of the best experts on this subject based on the ideXlab platform.
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the acute effects of single dose orally administered doramectin eprinomectin and selamectin on natural infections of Syphacia muris in rats
Experimental Parasitology, 2009Co-Authors: Feride Kircali Sevimli, Esma Kozan, Alper Sevimli, Nurhan Dogan, Aziz BulbulAbstract:Abstract This study was designed to determine the acute effects of a single-dose of orally administered doramectin, eprinomectin and selamectin on Syphacia muris infection in rats. Rats, naturally infected with S. muris , were divided into four groups: three different treatment groups ( n = 7) and one positive control ( n = 7). Cellophane tape preparations were obtained from the treated rats on day 0 pre-treatment and on days 2, 4 and 6 post-treatment. Syphacia sp. eggs were counted. Eprinomectin was found to be 100% effective in eliminating eggs on two post-treatment. However when egg counts on day 6 post-treatment were compared with pre-treatment egg counts, doramectin and selamectin were found to be 99.32 and 98.77% effective in eliminating eggs, respectively. On day 7 post-treatment, blood samples were obtained from all groups, and then the rats were necropsied. Doramectin, eprinomectin and selamectin were found to be 100% effective in eliminating adult S. muris , when compared with the positive control group.
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the acute effects of single dose orally administered doramectin eprinomectin and selamectin on natural infections of Syphacia muris in rats
Experimental Parasitology, 2009Co-Authors: Feride Kircali Sevimli, Esma Kozan, Alper Sevimli, Nurhan Dogan, Aziz BulbulAbstract:Abstract This study was designed to determine the acute effects of a single-dose of orally administered doramectin, eprinomectin and selamectin on Syphacia muris infection in rats. Rats, naturally infected with S. muris , were divided into four groups: three different treatment groups ( n = 7) and one positive control ( n = 7). Cellophane tape preparations were obtained from the treated rats on day 0 pre-treatment and on days 2, 4 and 6 post-treatment. Syphacia sp. eggs were counted. Eprinomectin was found to be 100% effective in eliminating eggs on two post-treatment. However when egg counts on day 6 post-treatment were compared with pre-treatment egg counts, doramectin and selamectin were found to be 99.32 and 98.77% effective in eliminating eggs, respectively. On day 7 post-treatment, blood samples were obtained from all groups, and then the rats were necropsied. Doramectin, eprinomectin and selamectin were found to be 100% effective in eliminating adult S. muris , when compared with the positive control group.
Handan Aydin - One of the best experts on this subject based on the ideXlab platform.
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use of topical selamectin for the treatment of Syphacia muris infection in laboratory rats
Polish Journal of Veterinary Sciences, 2008Co-Authors: Meltem Ulutaş Esatgil, Aynur Gulanber, Handan AydinAbstract:: Efficacy of selamectin was studied in naturally acquired S. muris infections in rats. Fourty-eight S. muris-positive rats were divided into six treated and two control groups. Selamectin (6 mg/kg) was applied topically to the skin in a single spot at the base of the neck in the treatment group. The rats of treated and control groups were necropsied on the 24th day after the treatment. Topical selamectin was found to be 40.7-63.3% effective (based on egg per gram method) in eliminating S. muris infection in rats. The efficacy of the treatment against S. muris (based on adult worm counts) in male and female rats was 35.14-58.88%, respectively (mean 48.39%).
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use of topical selamectin for the treatment of Syphacia muris infection in laboratory rats
Polish Journal of Veterinary Sciences, 2008Co-Authors: Meltem Ulutaş Esatgil, Aynur Gulanber, Handan AydinAbstract:: Efficacy of selamectin was studied in naturally acquired S. muris infections in rats. Fourty-eight S. muris-positive rats were divided into six treated and two control groups. Selamectin (6 mg/kg) was applied topically to the skin in a single spot at the base of the neck in the treatment group. The rats of treated and control groups were necropsied on the 24th day after the treatment. Topical selamectin was found to be 40.7-63.3% effective (based on egg per gram method) in eliminating S. muris infection in rats. The efficacy of the treatment against S. muris (based on adult worm counts) in male and female rats was 35.14-58.88%, respectively (mean 48.39%).
