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Jueitang Cheng - One of the best experts on this subject based on the ideXlab platform.
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release of acetylcholine by Syringin an active principle of eleutherococcus senticosus to raise insulin secretion in wistar rats
Neuroscience Letters, 2008Co-Authors: Yang Chang Wu, Wen Chen Yu, Jueitang ChengAbstract:Abstract The present study is designed to screen the effect of Syringin, an active principle purified from the rhizome and root parts of Eleutherococcus senticosus (Araliaceae), on the plasma glucose and investigate the possible mechanisms. Plasma glucose decreased in a dose-dependent manner 60 min after intravenous injection of Syringin into fasting Wistar rats. In parallel to the decrease of plasma glucose, increases of plasma insulin level as well as the plasma C-peptide was also observed in rats receiving same treatment. Both the plasma glucose lowering action and the raised plasma levels of insulin and C-peptide induced by Syringin were also inhibited by 4-diphenylacetoxy- N -methylpiperdine methiodide (4-DAMP), the antagonist of the muscarinic M 3 receptors, but not affected by the ganglionic nicotinic antagonist, pentolinium or hexamethonium. Moreover, disruption of synaptic available acetylcholine (ACh) using an inhibitor of choline uptake, hemicholinium-3, or vesicular acetylcholine transport, vesamicol, abolished these actions of Syringin. Also, physostigmine at concentration sufficient to inhibit acetylcholinesterase enhanced the actions of Syringin. Mediation of ACh release from the nerve terminals to enhance insulin secretion by Syringin can thus be considered. The results suggest that Syringin has an ability to raise the release of ACh from nerve terminals, which in turn to stimulate muscarinic M 3 receptors in pancreatic cells and augment the insulin release to result in plasma glucose lowering action.
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hypoglycemic effect of Syringin from eleutherococcus senticosus in streptozotocin induced diabetic rats
Planta Medica, 2008Co-Authors: Jueitang ChengAbstract:Abstract Eleutherococcus senticosus (Araliaceae ) is a very powerful adaptogenic agent. In the present study, the effects of Syringin, an active principle of this herb, on plasma glucose levels in streptozotocin-induced diabetic rats (STZ-diabetic rats) were investigated. Thirty minutes after Syringin was intravenously injected into fasting STZ-diabetic rats, plasma glucose levels dose-dependently decreased. In normal rats, Syringin at the effective dose (1.0 mg/kg) significantly attenuated the increase in plasma glucose caused by an intravenous glucose challenge. Syringin dose-dependently (0.01 to 10.0 micromol/L) stimulated glucose uptake in soleus muscle isolated from STZ-diabetic rats. Syringin treatment of hepatocytes isolated from STZ-diabetic rats enhanced glycogen synthesis . The ability of Syringin to enhance glucose utilization and lower plasma glucose level in rats suffering from insulin deficiency suggest that this chemical may be useful in the treatment of human diabetes.
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increase of β endorphin secretion by Syringin an active principle of eleutherococcus senticosus to produce antihyperglycemic action in type 1 like diabetic rats
Hormone and Metabolic Research, 2007Co-Authors: Jueitang ChengAbstract:: We employed streptozotocin-induced diabetic rats (STZ-diabetic rats) as type 1 diabetes-like animal models to investigate the mechanism(s) of antihyperglycemic action produced by Syringin, an active principle purified from the rhizome and root part S of ELEUTHEROCOCCUS SENTICOSUS (Araliaceae). Bolus intravenous (i. v.) injection of Syringin dose-dependently decreased the plasma glucose of STZ-diabetic rats in 30 minutes in a way parallel to the increase of plasma beta-endorphin-like immunoreactivity (BER). Syringin enhanced BER release from the isolated adrenal medulla of STZ-diabetic rats in a concentration-dependent manner from 0.001 to 10 micromol/l. Bilateral adrenalectomy in STZ-diabetic rats eliminated the activities of Syringin (1 mg/kg, i. v.) including the plasma glucose-lowering effect and the plasma BER-elevating effect. Also, Syringin failed to lower plasma glucose in the presence of micro-opioid receptor antagonists and/or in the micro-opioid receptor knockout diabetic mice. In conclusion, the obtained results suggest that Syringin can enhance the secretion of beta-endorphin from adrenal medulla to stimulate peripheral micro-opioid receptors resulting in a decrease of plasma glucose in diabetic rats lacking insulin.
Lei Yang - One of the best experts on this subject based on the ideXlab platform.
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Ionic liquid–lithium salt based microwave pretreatment followed by ultrasonic-assisted extraction of Syringin and oleuropein from Syringa reticulata var. mandshurica branch bark by a dual response surface methodology
Analytical Methods, 2016Co-Authors: Lianfei Zhao, Hua Wang, Huiyan Gu, Lei YangAbstract:Rapid and efficient solvent extraction of Syringin and oleuropein from Syringa reticulata var. mandshurica branch bark was achieved using microwave-assisted ionic liquid–lithium salt pretreatment, followed by ultrasonic breakdown of the plant cell walls. The conditions for this novel sample treatment method, including microwave pretreatment time and power, ultrasound irradiation extraction time and power, types and concentrations of ionic liquid and lithium salt, and the liquid–solid ratio, were optimized using a dual response surface methodology. The proposed approach under optimal conditions was compared with conventional extraction methods. No degradation of Syringin and oleuropein was observed in stability studies performed with a reference standard. The experimental results indicate that the proposed approach is a simple and efficient sample preparation technique that has strong potential for application in the field of sample analysis.
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ionic liquid lithium salt based microwave pretreatment followed by ultrasonic assisted extraction of Syringin and oleuropein from syringa reticulata var mandshurica branch bark by a dual response surface methodology
Analytical Methods, 2016Co-Authors: Lianfei Zhao, Hua Wang, Huiyan Gu, Lei YangAbstract:Rapid and efficient solvent extraction of Syringin and oleuropein from Syringa reticulata var. mandshurica branch bark was achieved using microwave-assisted ionic liquid–lithium salt pretreatment, followed by ultrasonic breakdown of the plant cell walls. The conditions for this novel sample treatment method, including microwave pretreatment time and power, ultrasound irradiation extraction time and power, types and concentrations of ionic liquid and lithium salt, and the liquid–solid ratio, were optimized using a dual response surface methodology. The proposed approach under optimal conditions was compared with conventional extraction methods. No degradation of Syringin and oleuropein was observed in stability studies performed with a reference standard. The experimental results indicate that the proposed approach is a simple and efficient sample preparation technique that has strong potential for application in the field of sample analysis.
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enrichment and purification of Syringin eleutheroside e and isofraxidin from acanthopanax senticosus by macroporous resin
International Journal of Molecular Sciences, 2012Co-Authors: Fengjian Yang, Lei Yang, Wenjie Wang, Chunjian Zhao, Yuangang ZuAbstract:In order to screen a suitable resin for the preparative simultaneous separation and purification of Syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for Syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol–water solution: 60:40 (v/v), eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of Syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively.
Tackmin Kwon - One of the best experts on this subject based on the ideXlab platform.
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Enhancement of Syringin contents in soybean seeds with seed-specific expression of a chimeric UGT72E3/E2 gene
Plant Biotechnology Reports, 2017Co-Authors: Tackmin Kwon, HYUN SUK CHO, Su Yeong Yun, Jeongbeom Kim, Jaesung Nam, Hye Jeong Kim, Young Soo ChungAbstract:Syringin, sinapyl alcohol 4-O-glucoside, is well known as a plant-derived bioactive monolignol glucoside. In Arabidopsis, recombinant chimeric protein UGT72E3/2 has been previously reported to lead to significantly higher Syringin production than the parental enzymes UGT72E2 and UGT72E3. To enhance Syringin content in Korean soybean (Glycine max L. ‘Kwangan’), we cloned the UGT72E3/2 gene under the control of the β-conglycinin or CaMV-35S promoter to generate β-UGT72E3/2 and 35S-UGT72E3/2 constructs, respectively, and then transformed them into soybean to obtain transgenic plants using the modified half-seed method. Real-time semi-quantitative PCR (RT-PCR) analysis showed that the UGT72E3/2 gene was expressed in the leaves of the β-UGT72E3/2 and 35S-UGT72E3/2 transgenic lines. HPLC analysis of the seeds and mature tissues of the T2 generation plants revealed that the β-UGT72E3/2 transgenic seeds accumulated 0.15 µmol/g DW of total Syringin and 0.29 µmol/g DW of total coniferin, whereas coniferin and Syringin were not detected in non-transgenic seeds. Moreover, coniferin and Syringin also accumulated at high levels in non-seed tissues, particularly the leaves of β-UGT72E3/2 transgenic lines. In contrast, 35S-UGT72E3/2 lines showed no differences in the contents of coniferin and Syringin between transgenic and non-transgenic soybean plants. Thus, the seed-specific β-conglycinin promoter might be an effective tool to apply to the nutritional enhancement of soybean crops through increased Syringin production.
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enhancement of Syringin contents in soybean seeds with seed specific expression of a chimeric ugt72e3 e2 gene
Plant Biotechnology Reports, 2017Co-Authors: Tackmin Kwon, Young Soo ChungAbstract:Syringin, sinapyl alcohol 4-O-glucoside, is well known as a plant-derived bioactive monolignol glucoside. In Arabidopsis, recombinant chimeric protein UGT72E3/2 has been previously reported to lead to significantly higher Syringin production than the parental enzymes UGT72E2 and UGT72E3. To enhance Syringin content in Korean soybean (Glycine max L. ‘Kwangan’), we cloned the UGT72E3/2 gene under the control of the β-conglycinin or CaMV-35S promoter to generate β-UGT72E3/2 and 35S-UGT72E3/2 constructs, respectively, and then transformed them into soybean to obtain transgenic plants using the modified half-seed method. Real-time semi-quantitative PCR (RT-PCR) analysis showed that the UGT72E3/2 gene was expressed in the leaves of the β-UGT72E3/2 and 35S-UGT72E3/2 transgenic lines. HPLC analysis of the seeds and mature tissues of the T2 generation plants revealed that the β-UGT72E3/2 transgenic seeds accumulated 0.15 µmol/g DW of total Syringin and 0.29 µmol/g DW of total coniferin, whereas coniferin and Syringin were not detected in non-transgenic seeds. Moreover, coniferin and Syringin also accumulated at high levels in non-seed tissues, particularly the leaves of β-UGT72E3/2 transgenic lines. In contrast, 35S-UGT72E3/2 lines showed no differences in the contents of coniferin and Syringin between transgenic and non-transgenic soybean plants. Thus, the seed-specific β-conglycinin promoter might be an effective tool to apply to the nutritional enhancement of soybean crops through increased Syringin production.
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enzymatic and metabolic engineering for efficient production of Syringin sinapyl alcohol 4 o glucoside in arabidopsis thaliana
Phytochemistry, 2014Co-Authors: Tackmin KwonAbstract:Abstract To promote efficient production of Syringin, a plant-derived bioactive monolignol glucoside, synergistic effects of enzymatic and metabolic engineering were combined. Recombinant UGT72E3 / E2 chimeras, generated by exchanging parts of the C-terminal domain including the Putative Secondary Plant Glycosyltransferase (PSPG) motif of UGT72E3 and UGT72E2, were expressed in leaves of transgenic Arabidopsis plants; Syringin production was measured in vivo and by enzymatic assays in vitro . In both tests, UGT72E3/2 displayed substrate specificity for sinapyl alcohol like the parental enzyme UGT72E3, and the Syringin production was significantly increased compared to UGT72E3. In particular, in the in vitro assay, which was performed in the presence of a high concentration of sinapyl alcohol, the production of Syringin by UGT72E3/2 was 4-fold higher than by UGT72E3. Furthermore, to enhance metabolic flow through the phenylpropanoid pathway and maintain a high basal concentration of sinapyl alcohol in the leaves, UGT72E3 / 2 was combined with the sinapyl alcohol synthesis pathway gene F5H encoding ferulate 5-hydroxylase and the lignin biosynthesis transcriptional activator MYB58 . The resulting UGT72E3/2+F5H+MYB58 OE plants, which simultaneously overexpress these three genes, accumulated a 56-fold higher level of Syringin in their leaves than wild-type plants.
Ridao Chen - One of the best experts on this subject based on the ideXlab platform.
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regulation of Syringin chlorogenic acid and 1 5 dicaffeoylquinic acid biosynthesis in cell suspension cultures of saussurea involucrata
China journal of Chinese materia medica, 2014Co-Authors: Ridao Chen, Lin YangAbstract:: Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid are three main bioactive ingredients in herbs of Saussurea involucrata with various pharmacological properties, while their contents are very low. In this study, the biosynthesis of Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid in the cell suspension cultures of S. involucrata were regulated by feeding carbon sources and precursors, which resulted in a great increase of the contents and yields of the above three bioactive ingredients. After 16 days of fermentation, the yields of Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid reached 339.0, 225.3, 512.7 mg x L(-1), respectively. Meanwhile, their contents increased up to 67.9, 1.9, 10.6 times of wild medicinal material, respectively. The results provided a solid basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compounds Syringin, chlorogenic acid and 1,5-dicaffeoylquinic acid.
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isolation and analysis of Syringin in cell suspension cultures of saussurea involucrata
Acta pharmaceutica Sinica, 2009Co-Authors: Ridao ChenAbstract:: Syringin is one of the main bioactive ingredients in Saussurea involucrata. In this study, various chromatographic techniques were employed to isolate and purify Syringin in the polar extraction of cell suspension cultures of S. involucrata. The structure of Syringin was characterized by the analysis of spectroscopic data. A quantitative analytical method for the content of Syringin in cultures of S. involucrata was established with RP-HPLC. The method is convenient, accurate and reliable. All this results provided a basis for further studies on application of cell suspension cultures of S. involucrata for large-scale production of bioactive compound Syringin.
Yuangang Zu - One of the best experts on this subject based on the ideXlab platform.
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enrichment and purification of Syringin eleutheroside e and isofraxidin from acanthopanax senticosus by macroporous resin
International Journal of Molecular Sciences, 2012Co-Authors: Fengjian Yang, Lei Yang, Wenjie Wang, Chunjian Zhao, Yuangang ZuAbstract:In order to screen a suitable resin for the preparative simultaneous separation and purification of Syringin, eleutheroside E and isofraxidin from Acanthopanax senticosus, the adsorption and desorption properties of 17 widely used commercial macroporous resins were evaluated. According to our results, HPD100C, which adsorbs by the molecular tiers model, was the best macroporous resin, offering higher adsorption and desorption capacities and higher adsorption speed for Syringin, eleutheroside E and isofraxidin than other resins. Dynamic adsorption and desorption tests were carried out to optimize the process parameters. The optimal conditions were as follows: for adsorption, processing volume: 24 BV, flow rate: 2 BV/h; for desorption, ethanol–water solution: 60:40 (v/v), eluent volume: 4 BV, flow rate: 3 BV/h. Under the above conditions, the contents of Syringin, eleutheroside E and isofraxidin increased 174-fold, 20-fold and 5-fold and their recoveries were 80.93%, 93.97% and 93.79%, respectively.
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the seasonal dynamics of the content of Syringin and total flavonoid in different parts of acanthopanax senticosus
Chinese Bulletin of Botany, 2006Co-Authors: Zehai Zhao, Qingyong Li, Yuangang ZuAbstract:In this paper, the contents of Syringin and total flavonoid in different parts of Acanthopanax senticosus according to seasonal dynamics were determined. Syringin mainly exists in branches, stems, rhizomes and roots, but in branches, the average content is 2.94%, which is the highest in all parts of A. secticosus. The Syringin content of the aboveground parts shows a distinct seasonal dynamics. It is higher in the spring and autumn but lower in the growth season （summer）. However, the Syringin content of underground parts does not show a distinct seasonal dynamics. All parts of A. senticosus contain flavonoid. The content of total flavonoid in the leaves shows a distinct dynamics. The highest content, about 3.8%, occurs in the middle of May before the forest canopy closure. The content of total flavonoid in branches and stems also shows a distinct seasonal dynamics： higher in the spring and autumn, but lower in summer. In the branches, the average content of total flavonoid reaches 3.75% in autumn. But the total flavonoid content of the underground parts does not show a distinct seasonal dynamic. The reasons for the seasonal dynamics of the Syringin and total flavonoid of A. senticosus are discussed.
