The Experts below are selected from a list of 345696 Experts worldwide ranked by ideXlab platform
Yanduo Tao - One of the best experts on this subject based on the ideXlab platform.
-
target separation of Flavonoids from saxifraga tangutica using two dimensional hydrophilic interaction chromatography reversed phase liquid chromatography
IEEE Journal of Solid-state Circuits, 2018Co-Authors: Jun Dang, Qilan Wang, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Li Zhang, Lijuan Mei, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
-
Target separation of Flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography.
Journal of Separation Science, 2018Co-Authors: Jun Dang, Qilan Wang, Mei Lijuan, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
Jun Dang - One of the best experts on this subject based on the ideXlab platform.
-
target separation of Flavonoids from saxifraga tangutica using two dimensional hydrophilic interaction chromatography reversed phase liquid chromatography
IEEE Journal of Solid-state Circuits, 2018Co-Authors: Jun Dang, Qilan Wang, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Li Zhang, Lijuan Mei, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
-
Target separation of Flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography.
Journal of Separation Science, 2018Co-Authors: Jun Dang, Qilan Wang, Mei Lijuan, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
Ulrike Mathesius - One of the best experts on this subject based on the ideXlab platform.
-
the role of Flavonoids in root rhizosphere signalling opportunities and challenges for improving plant microbe interactions
Journal of Experimental Botany, 2012Co-Authors: Samira Hassan, Ulrike MathesiusAbstract:The Flavonoid pathway produces a diverse array of plant compounds with functions in UV protection, as antioxidants, pigments, auxin transport regulators, defence compounds against pathogens and during signalling in symbiosis. This review highlights some of the known function of Flavonoids in the rhizosphere, in particular for the interaction of roots with microorganisms. Depending on their structure, Flavonoids have been shown to stimulate or inhibit rhizobial nod gene expression, cause chemoattraction of rhizobia towards the root, inhibit root pathogens, stimulate mycorrhizal spore germination and hyphal branching, mediate allelopathic interactions between plants, affect quorum sensing, and chelate soil nutrients. Therefore, the manipulation of the Flavonoid pathway to synthesize specifically certain products has been suggested as an avenue to improve root‐rhizosphere interactions. Possible strategies to alter Flavonoid exudation to the rhizosphere are discussed. Possible challenges in that endeavour include limited knowledge of the mechanisms that regulate Flavonoid transport and exudation, unforeseen effects of altering parts of the Flavonoid synthesis pathway on fluxes elsewhere in the pathway, spatial heterogeneity of Flavonoid exudation along the root, as well as alteration of Flavonoid products by microorganisms in the soil. In addition, the overlapping functions of many Flavonoids as stimulators of functions in one organism and inhibitors of another suggests caution in attempts to manipulate Flavonoid rhizosphere signals.
-
Differing requirements for Flavonoids during the formation of lateral roots, nodules and root knot nematode galls in Medicago truncatula.
New Phytologist, 2009Co-Authors: Anton P. Wasson, Kerry Ramsay, Michael G. K. Jones, Ulrike MathesiusAbstract:In this study, we tested whether the organogenesis of symbiotic root nodules, lateral roots and root galls induced by parasitic root knot nematodes (Meloidogyne javanica) was regulated by the presence of Flavonoids in the roots of Medicago truncatula. Flavonoids accumulate in all three types of root organ, and have been hypothesized previously to be required for secondary root organogenesis because of their potential role as auxin transport regulators. • Using RNA interference to silence the Flavonoid biosynthetic pathway in M. truncatula, we generated transformed Flavonoid-deficient hairy roots which were used to study Flavonoid accumulation, cell division and organogenesis of nodules, lateral roots and root galls. • Flavonoid-deficient roots did not form nodules, as demonstrated previously, but showed altered root growth in response to rhizobia. By contrast, Flavonoid-deficient roots showed no difference in the number of lateral roots and root galls. Galls on Flavonoid-deficient roots formed normal giant cells, but were shorter, and were characterized by reduced numbers of dividing pericycle cells. • We rejected the hypothesis that Flavonoids are required as general regulators of the organogenesis of secondary root organs, but Flavonoids appear to be necessary for nodulation. Possible reasons for this difference in the requirement for Flavonoids are discussed.
-
silencing the Flavonoid pathway in medicago truncatula inhibits root nodule formation and prevents auxin transport regulation by rhizobia
The Plant Cell, 2006Co-Authors: Anton Wasson, Flavia Pellerone, Ulrike MathesiusAbstract:Legumes form symbioses with rhizobia, which initiate the development of a new plant organ, the nodule. Flavonoids have long been hypothesized to regulate nodule development through their action as auxin transport inhibitors, but genetic proof has been missing. To test this hypothesis, we used RNA interference to silence chalcone synthase (CHS), the enzyme that catalyzes the first committed step of the Flavonoid pathway, in Medicago truncatula. Agrobacterium rhizogenes transformation was used to create hairy roots that showed strongly reduced CHS transcript levels and reduced levels of Flavonoids in silenced roots. Flavonoid-deficient roots were unable to initiate nodules, even though normal root hair curling was observed. Nodule formation and Flavonoid accumulation could be rescued by supplementation of plants with the precursor Flavonoids naringenin and liquiritigenin. The Flavonoid-deficient roots showed increased auxin transport compared with control roots. Inoculation with rhizobia reduced auxin transport in control roots after 24 h, similar to the action of the auxin transport inhibitor N-(1-naphthyl)phthalamic acid (NPA). Rhizobia were unable to reduce auxin transport in Flavonoid-deficient roots, even though NPA inhibited auxin transport. Our results present genetic evidence that root Flavonoids are necessary for nodule initiation in M. truncatula and suggest that they act as auxin transport regulators.
Qingbo Gao - One of the best experts on this subject based on the ideXlab platform.
-
target separation of Flavonoids from saxifraga tangutica using two dimensional hydrophilic interaction chromatography reversed phase liquid chromatography
IEEE Journal of Solid-state Circuits, 2018Co-Authors: Jun Dang, Qilan Wang, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Li Zhang, Lijuan Mei, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
-
Target separation of Flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography.
Journal of Separation Science, 2018Co-Authors: Jun Dang, Qilan Wang, Mei Lijuan, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
Yun Shao - One of the best experts on this subject based on the ideXlab platform.
-
target separation of Flavonoids from saxifraga tangutica using two dimensional hydrophilic interaction chromatography reversed phase liquid chromatography
IEEE Journal of Solid-state Circuits, 2018Co-Authors: Jun Dang, Qilan Wang, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Li Zhang, Lijuan Mei, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.
-
Target separation of Flavonoids from Saxifraga tangutica using two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography.
Journal of Separation Science, 2018Co-Authors: Jun Dang, Qilan Wang, Mei Lijuan, Huilan Yue, Zenggen Liu, Yun Shao, Qingbo Gao, Yanduo TaoAbstract:An orthogonally (80.3%) preparative two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography method has been established for the isolation and purification of Flavonoids from Saxifraga tangutica. Initially, Flavonoids were enriched by means of a middle-pressure chromatographic tower (containing middle chromatogram isolated gel). In the first dimension, a XION preparative column was used to separate the Flavonoid fractions under the guidance of characteristic ultraviolet absorption spectra of Flavonoids and nine Flavonoid fractions were obtained. Then, the coeluted Flavonoid fractions were selected for further purification via reversed-phase liquid chromatography with the parent ion peak of quercetin (303), kaempferol (287), or isorhamnetin (317). Several Flavonoids could be separated from each hydrophilic interaction chromatography fraction; furthermore, Flavonoids with poor resolution in one-dimensional liquid chromatography were isolated in two-dimensional liquid chromatography due to the orthogonality. In addition, this technique was valuable for trace Flavonoids, which were concentrated in the first stage and separated in the second stage. In total, 18 Flavonoids with either quercetin, kaempferol, or isorhamnetin parent nuclei were targetedly obtained, and 15 Flavonoids were obtained for the first time from S. tangutica. These results established that the off-line two-dimensional hydrophilic interaction chromatography/reversed-phase liquid chromatography technique was efficient for the isolation of Flavonoids from Saxifraga tangutica.