Taenia Crassiceps

14,000,000 Leading Edge Experts on the ideXlab platform

Scan Science and Technology

Contact Leading Edge Experts & Companies

Scan Science and Technology

Contact Leading Edge Experts & Companies

The Experts below are selected from a list of 1176 Experts worldwide ranked by ideXlab platform

Luis I. Terrazas - One of the best experts on this subject based on the ideXlab platform.

  • Taenia Crassiceps antigens control experimental type 1 diabetes by inducing alternatively activated macrophages
    Mediators of Inflammation, 2017
    Co-Authors: Arlett Espinozajimenez, Roberto De Haro, Luis I. Terrazas
    Abstract:

    Type 1 diabetes (T1D) is an autoimmune disease caused by the selective destruction of the pancreatic β-cells, causing inability to produce insulin. Proinflammatory cytokines such as IL-1β, IL-6, TNF-α, IFN-γ, IL-12, IL-17, and NO can be released by CD4 and CD8+ lymphocytes as well as by classically activated macrophages (CAMϕs), which are important in the development of T1D. Helminth infections have been shown to prevent T1D, mainly through Th2-biased responses and increased recruitment of regulatory cell populations. Previously, we have shown that Taenia Crassiceps infection in mice significantly reduces hyperglycemia, insulitis, and the incidence of T1D. In this study, we determined whether T. Crassiceps-derived products such as soluble (TcS) or excreted/secreted (TcES) antigens might have a beneficial influence on the development of experimental T1D. Treatment with different doses before or after induction of T1D was analyzed. Mice that were pretreated with TcS were unable to develop T1D, whereas those receiving TcES early after T1D induction displayed significantly reduced insulitis and hyperglycemia along with increased recruitment of alternatively activated macrophages (AAMϕs) and myeloid-derived suppressor cells (MDSCs). Finally, we examined the modulatory role of AAMϕs on T1D by depleting macrophages with clodronate-loaded liposomes, demonstrating that AAMϕs are key cells in T1D regulation.

  • Review Article Immunoregulation by Taenia Crassiceps and Its Antigens
    2016
    Co-Authors: Alberto N Peon, Arlett Espinoza-jiménez, Luis I. Terrazas
    Abstract:

    Copyright © 2013 Alberto N. Peón et al. is is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Taenia Crassiceps is a cestode parasite of rodents (in its larval stage) and canids (in its adult stage) that can also parasitize immunocompromised humans.We have studied the immune response elicited by this helminth and its antigens inmice and human cells, and have discovered that they have a strong capacity to induce chronic 2-type responses that are primarily characterized by high levels of 2 cytokines, low proliferative responses in lymphocytes, an immature and LPS-tolerogenic pro�le in dendritic cells, the recruitment of myeloid-derived suppressor cells and, specially, alternatively activated macrophages. We also have utilized the immunoregulatory capabilities of this helminth to successfully modulate autoimmune responses and the outcome of other infectious diseases. In the present paper, we review the work of others and ourselves with regard to the immune response induced by T. Crassiceps and its antigens, and we compare the advances in our understanding of this parasitic infection model with the knowledge that has been obtained from other selected models. 1

  • immunoregulation by Taenia Crassiceps and its antigens
    BioMed Research International, 2013
    Co-Authors: Alberto N Peon, Arlett Espinozajimenez, Luis I. Terrazas
    Abstract:

    Taenia Crassiceps is a cestode parasite of rodents (in its larval stage) and canids (in its adult stage) that can also parasitize immunocompromised humans. We have studied the immune response elicited by this helminth and its antigens in mice and human cells, and have discovered that they have a strong capacity to induce chronic Th2-type responses that are primarily characterized by high levels of Th2 cytokines, low proliferative responses in lymphocytes, an immature and LPS-tolerogenic profile in dendritic cells, the recruitment of myeloid-derived suppressor cells and, specially, alternatively activated macrophages. We also have utilized the immunoregulatory capabilities of this helminth to successfully modulate autoimmune responses and the outcome of other infectious diseases. In the present paper, we review the work of others and ourselves with regard to the immune response induced by T. Crassiceps and its antigens, and we compare the advances in our understanding of this parasitic infection model with the knowledge that has been obtained from other selected models.

  • Taenia Crassiceps infection does not influence the development of experimental rheumatoid arthritis
    BioMed Research International, 2013
    Co-Authors: Aaxin M Ortizflores, Miriam Rodriguezsosa, Yadira Ledesmasoto, Elsa A Calleja, Imelda Juarez, Luis I. Terrazas
    Abstract:

    It was previously reported by our group that infection with Taenia Crassiceps reduces incidence and severity of inflammatory and autoimmune experimental diseases like type 1 diabetes and experimental autoimmune encephalomyelitis. In this research, we set out to study whether infection with T. Crassiceps would affect the development of experimental rheumatoid arthritis (RA). We found that mice infected with the parasite and induced with experimental RA showed similar clinical scores as the noninfected experimental RA group; systemic cytokines were not affected while anti-CII Abs were higher in the infected group. Histological evaluation showed damage in both infected and noninfected experimental RA-induced groups and although some surface molecules such as PDL-2 and MR which are associated with immunomodulatory mechanisms were upregulated in the infected and RA-induced group as compared to the noninfected RA group, they did not exert any changes in the outcome of experimental RA. Thus, we determined that infection with T. Crassiceps does not influence the outcome of experimental RA.

  • a possible tlr2 medited immunity to experimental cysticercosis caused by Taenia Crassiceps 58 3
    Journal of Immunology, 2011
    Co-Authors: Luis I. Terrazas, José L. Reyes, Marisol I Gonzalezgonzalez, Abhay R. Satoskar
    Abstract:

    TLR2 is a key molecule required for innate immunity and is involved in the recognition of a wide range of pathogens from bacteria, fungi, parasites and viruses. Its role on immunity to cestode infections is poorly understood. Here the role of TLR2 was evaluated in a Taenia Crassiceps infection model. Compared with wild-type mice, TLR2 knockout (KO) mice failed to produce significant levels of inflammatory cytokines in both sera and their spleens during the first two weeks of Taenia infection. TLR2 KO mice developed a Th2-dominant whereas wild-type mice developed a Th1-dominant immune response after Taenia infection. Associated with the insufficient production of early inflammatory cytokines and lack of Th1-dominant adaptive immunity, TLR2 KO mice developed significantly higher numbers of parasites whereas WT mice appeared to be resistant. TLR2 KO mice developed more amounts of alternatively activated macrophages. These results suggest that TLR2-dependent signaling pathway is not only required for inflammatory cytokine production in the early phase of host response to T. Crassiceps infection but also that plays a critical role in the development of Th1-adaptive immunity, both of which may be essential for limiting infection and reducing pathology during experimental cysticercosis. Collectively, these studies indicate that TLR2 is involved in T. Crassiceps recognition and subsequent innate immune activation.

Carlos Larralde - One of the best experts on this subject based on the ideXlab platform.

  • development of the s3pvac vaccine against murine Taenia Crassiceps cysticercosis a historical review
    Journal of Parasitology, 2013
    Co-Authors: Edda Sciutto, Marisela Hernandez, Gladis Fragoso, Gabriela Rosas, J J Martinez, Agnes Fleury, Jacquelynne Cervantes, Aline S De Aluja, Carlos Larralde
    Abstract:

    Our work of the last 25 yr was concerned with the development of a vaccine aimed to prevent porcine Taenia solium cysticercosis and was based on cross-reacting Taenia Crassiceps antigens that had proved protective against experimental intraperitoneal murine T. Crassiceps cysticercosis (EIMTcC). In recent times the efficacy of the vaccine has been considered in need of confirmation, and the use of EIMTcC has been questioned as a valid tool in screening for vaccine candidates among the many antigens possibly involved. A review of our work divided in 2 parts is presented at this point, the first dealing with EIMTcC and the second with porcine T. solium cysticercosis (presented in this issue). Herein, we revise our results using EIMTcC as a measure of the protective capacity of T. Crassiceps complex antigen mixtures, of purified native antigens, and of S3Pvac anti-cysticercosis vaccine composed by 3 protective peptides: GK-1, KETc1, and KETc12 either synthetic or recombinantly expressed and collectively or separately, by diverse delivery systems when administered at different doses and by different routes. Statistical analyses of the data lead confidently to the strong inference that S3Pvac is indeed an effective vaccine against EIMTcC via specific and non-specific mechanisms of protection.

  • budding of Taenia Crassiceps cysticerci in vitro is promoted by crowding in addition to hormonal stress and energy related signals
    BioMed Research International, 2010
    Co-Authors: Pedro Ostoasaloma, Pedro Ostoajacobo, Marcela Esquivelvelazquez, Silvana Bazua, Carlos Larralde
    Abstract:

    Taenia Crassiceps cysticerci (cysts) reproduce by budding. The cysts' production of buds was measured in vitro to explore parasite and environmental-related factors involved in the extreme individual variation in parasite loads of inbred mice. Cysts were placed in in vitro culture for 10 days at initial parasite densities of 1, 5, 10 cysts/well in 1 ml of RPMI Medium 1640 without serum. Results showed that there is considerable intrinsic initial variation among inoculated cysts in their production of buds and that increasing parasite density (crowding) stimulates the overall production of buds and recruit into budding most of the cysts. Identical cultures were then subjected to various treatments such as heating and exposure to peroxide to induce stress, or to 17s-estradiol, insulin, glucose, or insulin+glucose to supplement putatively limiting hormonal and energy resources. All treatments increased budding but the parasites' strong budding response to crowding alone overshadows the other treatments.

  • treatment with dehydroepiandrosterone in vivo and in vitro inhibits reproduction growth and viability of Taenia Crassiceps metacestodes
    International Journal for Parasitology, 2008
    Co-Authors: Jose Antonio Vargasvillavicencio, Carlos Larralde, Jorge Moralesmontor
    Abstract:

    The aim of this work was to explore the effect of dehydroepiandrosterone (DHEA) on the establishment, growth and reproduction of the metacestode stage of the tapeworm Taenia Crassiceps, both in vivo and in vitro. Administration of DHEA prior to infection in mice of both sexes reduced the parasite load by 50% compared with untreated mice. This protective effect was not associated with the immune response, since there was no effect of DHEA treatment on mRNA levels of IL-2, IFN-gamma, IL-4 or IL-10. DHEA treatment of infected mice increased androgen receptor expression in splenocytes of both sexes. Moreover, in vitro treatment of T. Crassiceps with DHEA reduced reproduction, motility and viability in a dose- and time-dependent fashion. Results indicate that DHEA has strong negative direct modulatory effects on murine cysticercosis. We suggest the use of hormonal-analogues for protective purposes as a therapeutic approach to prevent murine cysticercosis.

  • preferential growth of Taenia Crassiceps cysticerci in female mice holds across several laboratory mice strains and parasite lines
    Journal of Parasitology, 2008
    Co-Authors: Gladis Fragoso, Edda Sciutto, Agnes Fleury, Gabriela Castano Meneses, Carlos Larralde
    Abstract:

    A retrospective study of our 14-yr records on experimental Taenia Crassiceps (ORFfast line) cysticercosis (n = 1,198) shows that in 16 of 17 different mice strains, female mice are more frequently infected and carry larger individual parasite loads than males. However, sexual differences in parasite loads significantly varies between strains in relation to their different genetic backgrounds (BALB > C57Bl = OTHERS > C3H). The coefficient of variation in all female mice is significantly smaller than that of all males, an indication of males' more potent, but erratically effective, restraint of cysticercus growth. Similar positive growth bias for female mice is shown by other lines of cysticerci, i.e., HYGslow and WFUslow. These results contravene the usual expectation of female hosts being more resistant than males to parasite infections, and they point to the multiple factors that combined determine sex related differences of mice to experimental cysticercosis infection.

  • molecular mechanisms involved in the differential effects of sex steroids on the reproduction and infectivity of Taenia Crassiceps
    Journal of Parasitology, 2004
    Co-Authors: Galileo Escobedo, Carlos Larralde, Anahi Chavarria, Marco Cerbon, Jorge Moralesmontor
    Abstract:

    The in vitro exposure of Taenia Crassiceps cysticerci to 17-β estradiol (E2) and progesterone (P4) stimulated their reproduction and infectivity. Testosterone (T4) and dihydrotestosterone (DHT) inhibited their reproduction and reduced their motility and infectivity. E2 and P4 increased, whereas T4 and DHT reduced, the expression of parasite c-fos and c-jun and DNA synthesis. In vitro exposure of cysticerci to sex steroids before their inoculation into recipient noninfected mice resulted in large parasite loads when pretreated with E2 and P4 and in smaller loads when pretreated with T4 and DHT. To determine the possible molecular mechanisms by which sex steroids affect T. Crassiceps, sex steroid receptors were amplified. Taenia Crassiceps expressed estrogen receptors (both α and β isoforms) and androgen receptors but no P4 receptors. These results demonstrate that sex steroids act directly on parasite reproduction by binding to a classic and specific sex steroid receptor on the parasite. The differential r...

Gabriela Borges Da Silva - One of the best experts on this subject based on the ideXlab platform.

  • igy antibody and human neurocysticercosis a novel approach on immunodiagnosis using Taenia Crassiceps hydrophobic antigens
    Parasitology, 2020
    Co-Authors: Guilherme M P Carrara, Gabriela Borges Da Silva, Lucas Silva De Faria, Daniela Da Silva Nunes, Vanessa Da Silva Ribeiro, Camila De Carvalho Almanca Lopes, Maria Do Rosario De Fatima Goncalvespires, Malu M Santos, Isabela Pacheco Borges, Alvaro Ferreirajunior
    Abstract:

    Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia Crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. Crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.

  • Avaliação de frações antigênicas de Taenia Crassiceps obtidas por hidrofobicidade, no imunodiagnóstico das formas ativa e inativa da neurocisticercose humana em amostras de líquido cefalorraquidiano
    Universidade Federal de Uberlândia, 2016
    Co-Authors: Gabriela Borges Da Silva
    Abstract:

    A neurocisticercose (NCC) é uma parasitose que acomete o sistema nervoso central (SNC) humano e é causada pela forma metacestódea de Taenia solium. NCC é uma doença pleomórfica devido as diferenças no número e localização das lesões e gravidade da reação imune do hospedeiro contra o parasito. Apesar dos avanços na neuroimagem e testes imunológicos, o diagnóstico da NCC ainda é um desafio devido às manifestações clínicas inespecíficas. A dificuldade de obtenção dos metacestódeos, a partir de suínos naturalmente infectados, tem estimulado a busca por antígenos heterólogos, como os de Taenia Crassiceps, mantidos em modelo experimental. O estudo objetivou avaliar o extrato salino total de metacestódeos de T. Crassiceps (ES) e suas frações antigênicas (detergente - FD e aquosa - FA) obtidas por hidrofobicidade através do uso de Triton X-114, aplicadas em um método imunológico alternativo para o diagnóstico da NCC humana ativa e inativa em amostras de líquido cefalorraquidiano (LCR), método simples e de baixo custo. O extrato salino total de metacestódeos de T. Crassiceps e suas frações foram testados por ELISA (enzyme-linked immunosorbent assay) para detecção de imunoglobulinas G (IgG). FD e FA demonstraram altos índices de sensibilidade (Se) e especificidade (Es) no imunodiagnóstico da NCC ativa e inativa (100%) quando comparadas com o extrato salino total (Se = 100% e Es = 93%, ativa; Se= 95% e Es = 93%, inativa). Os resultados demonstraram que ambas as frações, FD e FA são importantes, eficientes e potencialmente aplicáveis no diagnóstico da NCC ativa e inativa.The neurocysticercosis (NCC) is parasitic disease that affects the human central nervous system (CNS) and is caused by metacestodes of Taenia solium. NCC is a pleomorphic disease due to differences in the number and location of the lesions and the severity of the immune reaction of the host against the parasite. Despite advances in neuroimaging and immunological tests, NCC diagnosis still is a challenge due to the nonspecific clinical manifestations. The difficulty of obtaining metacestodes of naturally infected pigs has stimulated the search for heterologous antigens, such as Taenia Crassiceps, kept in experimental model. The study aimed to evaluate the total extract of metacestodes of Taenia Crassiceps (SE) and its antigenic fractions (detergent - DF and aqueous - AF) obtained by hydrophobicity through the use of Triton X-114, applied in na alternative method for the immunological diagnosis of active and inactive human NCC in cerebrospinal fluid (CSF), a low cost and rapid technique. The total saline extract of metacestodes of T. Crassiceps and their antigenic fractions were tested by ELISA (enzyme-linked immunosorbent assay) for detection of immunoglobulin G (IgG). DF and AF showed high sensitivity (Se) and specificity (Sp) indexes in immunodiagnosis of active or inactive NCC (100% ) when compared with saline total extract (Se = 100% and Sp = 93%, active; Se = 95% and Sp = 93%, inactive). The results demonstrated that both fractions, DF and AF are important, efficient and potentially applicable in the diagnosis of active and inactive NC

  • Avaliação de frações antigênicas de Taenia Crassiceps obtidas por hidrofobicidade, no imunodiagnóstico das formas ativa e inativa da neurocisticercose humana em amostras de líquido cefalorraquidiano
    'EDUFU - Editora da Universidade Federal de Uberlandia', 2016
    Co-Authors: Gabriela Borges Da Silva
    Abstract:

    The neurocysticercosis (NCC) is parasitic disease that affects the human central nervous system (CNS) and is caused by metacestodes of Taenia solium. NCC is a pleomorphic disease due to differences in the number and location of the lesions and the severity of the immune reaction of the host against the parasite. Despite advances in neuroimaging and immunological tests, NCC diagnosis still is a challenge due to the nonspecific clinical manifestations. The difficulty of obtaining metacestodes of naturally infected pigs has stimulated the search for heterologous antigens, such as Taenia Crassiceps, kept in experimental model. The study aimed to evaluate the total extract of metacestodes of Taenia Crassiceps (SE) and its antigenic fractions (detergent - DF and aqueous - AF) obtained by hydrophobicity through the use of Triton X-114, applied in na alternative method for the immunological diagnosis of active and inactive human NCC in cerebrospinal fluid (CSF), a low cost and rapid technique. The total saline extract of metacestodes of T. Crassiceps and their antigenic fractions were tested by ELISA (enzyme-linked immunosorbent assay) for detection of immunoglobulin G (IgG). DF and AF showed high sensitivity (Se) and specificity (Sp) indexes in immunodiagnosis of active or inactive NCC (100% ) when compared with saline total extract (Se = 100% and Sp = 93%, active; Se = 95% and Sp = 93%, inactive). The results demonstrated that both fractions, DF and AF are important, efficient and potentially applicable in the diagnosis of active and inactive NCCFundação de Amparo a Pesquisa do Estado de Minas GeraisMestre em Imunologia e Parasitologia AplicadasA neurocisticercose (NCC) é uma parasitose que acomete o sistema nervoso central (SNC) humano e é causada pela forma metacestódea de Taenia solium. NCC é uma doença pleomórfica devido as diferenças no número e localização das lesões e gravidade da reação imune do hospedeiro contra o parasito. Apesar dos avanços na neuroimagem e testes imunológicos, o diagnóstico da NCC ainda é um desafio devido às manifestações clínicas inespecíficas. A dificuldade de obtenção dos metacestódeos, a partir de suínos naturalmente infectados, tem estimulado a busca por antígenos heterólogos, como os de Taenia Crassiceps, mantidos em modelo experimental. O estudo objetivou avaliar o extrato salino total de metacestódeos de T. Crassiceps (ES) e suas frações antigênicas (detergente - FD e aquosa - FA) obtidas por hidrofobicidade através do uso de Triton X-114, aplicadas em um método imunológico alternativo para o diagnóstico da NCC humana ativa e inativa em amostras de líquido cefalorraquidiano (LCR), método simples e de baixo custo. O extrato salino total de metacestódeos de T. Crassiceps e suas frações foram testados por ELISA (enzyme-linked immunosorbent assay) para detecção de imunoglobulinas G (IgG). FD e FA demonstraram altos índices de sensibilidade (Se) e especificidade (Es) no imunodiagnóstico da NCC ativa e inativa (100%) quando comparadas com o extrato salino total (Se = 100% e Es = 93%, ativa; Se= 95% e Es = 93%, inativa). Os resultados demonstraram que ambas as frações, FD e FA são importantes, eficientes e potencialmente aplicáveis no diagnóstico da NCC ativa e inativa

Javier R. Ambrosio - One of the best experts on this subject based on the ideXlab platform.

  • alterations in Taenia Crassiceps cysticerci cytoskeleton induced by nitazoxanide and flubendazole
    Acta Tropica, 2021
    Co-Authors: Nayana F Lima, Javier R. Ambrosio, Guaraciara De Andrade Picanco, Diana Gabriela Rios Valencia, Edgar Oliver Lopez Villegas, Maria Del Rosario Espinoza Mellado, Marina Clare Vinaud
    Abstract:

    Abstract Cysticercosis is the presence of Taenia solium larval stage in tissues such as central nervous system, skin, muscles and eye globe. The current treatment is based on albendazole and praziquantel which already present resistance reports. Therefore, the search for alternative treatments is paramount. The aim of this study was to determine the effect of flubendazole and nitazoxanide on cytoskeleton proteins from Taenia Crassiceps cysticerci, an experimental model for cysticercosis. Cysticerci were cultured in RPMI supplemented medium containing nitazoxanide and/or flubendazole. 24 h after the exposure the cysticerci were processed for scanning and transmission electron microscopy and for protein analysis of the cytoskeleton. The proteins were detected through 1D electrophoresis and identified through Western Blot. Nitazoxanide exposure increased tubulin and actin quantifications in T. Crassiceps cysticerci. While flubendazole alone and the drugs combinations induced an increase in α-tubulin and actin and decreased β-tubulin quantifications in the parasite. Morphological changes such as swelling and rupture of vesicle, stiff membrane, decrease in movements were observed when the cysticerci were incubated with the different compounds. In conclusion the drugs induced significative impact in the parasite`s cytoskeleton and may be considered as alternative treatments for cysticercosis.

  • metabolic effects of anthelminthic drugs in the larval stage of the cestode Taenia Crassiceps cysticercosis experimental model a review
    Acta Tropica, 2020
    Co-Authors: Marina Clare Vinaud, Javier R. Ambrosio
    Abstract:

    Taenia Crassiceps is an experimental model used for cysticercosis studies and has suffered metabolic analyzes regarding the effect of anthelminthic drugs. The metabolic analyses are useful tools to determine the drugs mode of action and the parasite`s survival mechanisms. The energetic pathways are good candidates for this kind of approach as they are essential for the parasite`s survival and adaptation to the environment. In this review we discuss the anthelminthic drugs mode of action and its metabolic impact on Taenia Crassiceps cysticerci.

  • proteomic profile associated with cell death induced by androgens in Taenia Crassiceps cysticerci proposed interactome
    Journal of Helminthology, 2019
    Co-Authors: Javier R. Ambrosio, Margarita Isabel Palaciosarreola, D G Riosvalencia, Olivia Reynosoducoing, Karen Elizabeth Navacastro, Pedro Ostoasaloma, Jorge Moralesmontor
    Abstract:

    Androgens have been shown to exert a cysticidal effect upon Taenia Crassiceps, an experimental model of cysticercosis. To further inquire into this matter, the Taenia Crassiceps model was used to evaluate the expression of several proteins after testosterone (T4) and dihydrotestosterone (DHT) in vitro treatment. Under 2-D proteomic maps, parasite extracts were resolved into approximately 130 proteins distributed in a molecular weight range of 10-250 kDa and isoelectrical point range of 3-10. The resultant proteomic pattern was analysed, and significant changes were observed in response to T4 and DHT. Based on our experience with electrophoretic patterns and proteomic maps of cytoskeletal proteins, alteration in the expression of isoforms of actin, tubulin and paramyosin and of other proteins was assessed. Considering that androgens may exert their biological activity in taeniids through the non-specific progesterone receptor membrane component (PGRMC), we harnessed bioinformatics to propose the identity of androgen-regulated proteins and establish their hypothetical physiological role in the parasites. These analyses yield a possible explanation of how androgens exert their cysticidal effects through changes in the expression of proteins involved in cytoskeletal rearrangement, dynamic vesicular traffic and transduction of intracellular signals.

  • a benzimidazole derivative rcb15 in vitro induces the alternative energetic metabolism and glycolysis in Taenia Crassiceps cysticerci
    Acta Tropica, 2017
    Co-Authors: Guaraciara A Picanco, Javier R. Ambrosio, Nayana F Lima, Carolina Miguel Fraga, Tatiane Luiza Da Costa, Eliana Isac, Marina Clare Vinaud
    Abstract:

    Abstract The emergence of resistance to albendazole has encouraged the search for effective alternatives for cysticercosis and other parasitosis treatment. RCB15 is a benzimidazole derivative that may be used against such diseases. The aim of this study was to determine the in vitro effect of RCB15 on the alternative energetic pathways of Taenia Crassiceps cysticerci. The cysticerci were in vitro exposed to albendazole sulphoxide (ABZSO) or RCB15 at different concentrations during 24 h. The cysticerci extract and the culture medium were analyzed through spectrophotometry and high performance liquid chromatography as to detect glucose, urea, creatinine and organic acids of the energetic metabolism. The drugs did not influence the protein catabolism. Fatty acids oxidation was enhanced through significantly higher acetate concentrations in the groups treated with RCB15 and ABZSO. Beta-hydroxybutyrate concentrations were decreased which indicates the use of fatty acids towards acetyl-CoA synthesis. There was a decrease in glucose uptake and pyruvate concentrations. The absence of lactate indicates the use of pyruvate in gluconeogenesis. Therefore it is possible to conclude that RCB15 enhanced the alternative energetic pathways of cysticerci in vitro exposed to different concentration, with emphasis on the fatty acids catabolism.

  • Dose-response curves of testosterone (T4) and dihydrotestosterone (DHT) effects on the motility and viability of cysticerci of Taenia Crassiceps ORF strain.
    2015
    Co-Authors: Javier R. Ambrosio, Olivia Reynoso-ducoing, Laura Valverde-islas, Galileo Escobedo, Karen E. Nava-castro, Isabel Palacios- M. Arreola, Pedro Ostoa-saloma, Azucena Ruíz-rosado, Lenin Dominguez-ramírez, Jorge Morales-montor
    Abstract:

    Data represents mean ± SD from 6 experiments with six wells by dose, and 10 cisticerci by well. The media and hormones were changed every other day, and cysticerci were in culture a total of 10 days.†P< 0.05,††P< 0.01 of statistical significance.Dose-response curves of testosterone (T4) and dihydrotestosterone (DHT) effects on the motility and viability of cysticerci of Taenia Crassiceps ORF strain.

Dennis Tappe - One of the best experts on this subject based on the ideXlab platform.

Related terms

Taenia Crassiceps - 15 days free trial to Access Experts & Abstracts