Taenia Multiceps

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Antonio Scala - One of the best experts on this subject based on the ideXlab platform.

  • Atypical outbreak of acute coenurosis by Taenia Multiceps in a sheep flock
    Parasitology Research, 2018
    Co-Authors: Davide Pintus, Antonio Varcasia, Claudia Tamponi, Maria Lucia Manunta, Giorgia Dessì, Giovanni Antonio Carboni, Maria Giovanna Cancedda, Ciriaco Ligios, Antonio Scala
    Abstract:

    Herein, we examined the brain of adult ewes and lambs less than 30 days old which were found affected by neurological signs in a flock located in Sardinia (Italy). Gross anatomo-pathological examination of all brains of the animals revealed multiple linear reddish-yellow foci of necrotic purulent inflammation due to oncosphere migration. Histologically, we confirmed a multifocal pyo-granulomatous meningo-encephalitis both in ewes and in lambs, confirming acute coenurosis. Morphological examination and DNA sequencing identified the Taenia Multiceps we isolated as Tm1 strain. This report describes for the first time a natural acute coenurosis infection in suckling lambs under 30 days of age.

  • RESEARCH Open Access Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Omnia Mohamed K, Maria Lucia Manunta, Antonio Scala
    Abstract:

    Background: Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. Methods: The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculate

  • Morphology and genetic variability within Taenia Multiceps in ruminants from Italy.
    Veterinary parasitology, 2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Anna Paola Pipia, Antonio Scala, Giorgia Dessì, A. Zidda, Marco Pau, Belgees Boufana
    Abstract:

    The aim of this study was to investigate the genetic variability and population structure of Taenia Multiceps, and to correlate morphological features of individual coenuri with haplotypes. A total of 92 animals (86 sheep; 4 goats; 1 cattle; 1 mouflon, Ovis musimon) aged between 6-36 months showing clinical symptoms of cerebral coenurosis were included in this study. T. Multiceps coenuri (n=118) sampled from live animals during routine surgery procedures or at post-mortem inspections were examined morphologically and molecularly identified. Morphological features of the 52 coenuri selected for this study (number and size of large and small hooks) were within the range reported in the literature. Fifty-two of the molecularly confirmed T. Multiceps coenuri harboured by 47 animals (sheep=41; cattle=1; goats=4; mouflon=1) were used to determine gene genealogies and population genetic indices and were compared to the 3 T. Multiceps genetic variants, Tm1-Tm3 previously described from Sardinia, Italy. For the 379 bp cox1 dataset we identified 11 polymorphic sites of which 8 were parsimony informative. A high haplotype diversity (0.664±0.067) was recorded for the cox1 sequences defining 10 haplotypes (TM01-TM10). The comparison of haplotypes generated in this study with published T. Multiceps Tm1 variant pointed to the possible existence of a common lineage for T. Multiceps. No correlation was detected between the size of the small and large hooks and the cox 1 haplotypes. Polycystic infestation (2-9 coenuri) was recorded in 27.7% of animals (13/47). No statistical correlation between polycystic T. Multiceps infection and haplotypes was detected.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    Parasites & Vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Background Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps , due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox ( Vulpes vulpes ) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox ( Vulpes vulpes ) in the epidemiology of T. Multiceps and related metacestodoses. Methods The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps . Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. Results A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps . Morphometrical features of the cysts were consistent with those of T. Multiceps ; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox 1 and Nd1) from the metacestode material confirmed the identification. Conclusions The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps
    Parasites & vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps. Morphometrical features of the cysts were consistent with those of T. Multiceps; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox1 and Nd1) from the metacestode material confirmed the identification. The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

Antonio Varcasia - One of the best experts on this subject based on the ideXlab platform.

  • Atypical outbreak of acute coenurosis by Taenia Multiceps in a sheep flock
    Parasitology Research, 2018
    Co-Authors: Davide Pintus, Antonio Varcasia, Claudia Tamponi, Maria Lucia Manunta, Giorgia Dessì, Giovanni Antonio Carboni, Maria Giovanna Cancedda, Ciriaco Ligios, Antonio Scala
    Abstract:

    Herein, we examined the brain of adult ewes and lambs less than 30 days old which were found affected by neurological signs in a flock located in Sardinia (Italy). Gross anatomo-pathological examination of all brains of the animals revealed multiple linear reddish-yellow foci of necrotic purulent inflammation due to oncosphere migration. Histologically, we confirmed a multifocal pyo-granulomatous meningo-encephalitis both in ewes and in lambs, confirming acute coenurosis. Morphological examination and DNA sequencing identified the Taenia Multiceps we isolated as Tm1 strain. This report describes for the first time a natural acute coenurosis infection in suckling lambs under 30 days of age.

  • RESEARCH Open Access Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Omnia Mohamed K, Maria Lucia Manunta, Antonio Scala
    Abstract:

    Background: Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. Methods: The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculate

  • Morphology and genetic variability within Taenia Multiceps in ruminants from Italy.
    Veterinary parasitology, 2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Anna Paola Pipia, Antonio Scala, Giorgia Dessì, A. Zidda, Marco Pau, Belgees Boufana
    Abstract:

    The aim of this study was to investigate the genetic variability and population structure of Taenia Multiceps, and to correlate morphological features of individual coenuri with haplotypes. A total of 92 animals (86 sheep; 4 goats; 1 cattle; 1 mouflon, Ovis musimon) aged between 6-36 months showing clinical symptoms of cerebral coenurosis were included in this study. T. Multiceps coenuri (n=118) sampled from live animals during routine surgery procedures or at post-mortem inspections were examined morphologically and molecularly identified. Morphological features of the 52 coenuri selected for this study (number and size of large and small hooks) were within the range reported in the literature. Fifty-two of the molecularly confirmed T. Multiceps coenuri harboured by 47 animals (sheep=41; cattle=1; goats=4; mouflon=1) were used to determine gene genealogies and population genetic indices and were compared to the 3 T. Multiceps genetic variants, Tm1-Tm3 previously described from Sardinia, Italy. For the 379 bp cox1 dataset we identified 11 polymorphic sites of which 8 were parsimony informative. A high haplotype diversity (0.664±0.067) was recorded for the cox1 sequences defining 10 haplotypes (TM01-TM10). The comparison of haplotypes generated in this study with published T. Multiceps Tm1 variant pointed to the possible existence of a common lineage for T. Multiceps. No correlation was detected between the size of the small and large hooks and the cox 1 haplotypes. Polycystic infestation (2-9 coenuri) was recorded in 27.7% of animals (13/47). No statistical correlation between polycystic T. Multiceps infection and haplotypes was detected.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    Parasites & Vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Background Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps , due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox ( Vulpes vulpes ) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox ( Vulpes vulpes ) in the epidemiology of T. Multiceps and related metacestodoses. Methods The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps . Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. Results A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps . Morphometrical features of the cysts were consistent with those of T. Multiceps ; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox 1 and Nd1) from the metacestode material confirmed the identification. Conclusions The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps
    Parasites & vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps. Morphometrical features of the cysts were consistent with those of T. Multiceps; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox1 and Nd1) from the metacestode material confirmed the identification. The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

Guangyou Yang - One of the best experts on this subject based on the ideXlab platform.

  • Expression and serodiagnostic potential of antigen B and thioredoxin peroxidase from Taenia Multiceps.
    Veterinary parasitology, 2019
    Co-Authors: Yuchen Liu, Yingdong Yang, Weimin Lai, Yue Xie, Bo Jing, Xiaowei Dong, Peng Xuerong, Guangyou Yang
    Abstract:

    Coenurosis is a serious parasitic disease of herbivorous animals caused by the metacestode of Taenia Multiceps (Coenurus cerebralis). Accordingly, a significant amount of research is currently dedicated to the development of appropriate antigens for use in rapid and accurate coenurosis diagnosis kits. In the present study, antigen B (AgB) and thioredoxin peroxidase (TPx) from T. Multiceps were cloned and expressed using a prokaryotic system, molecular characterization of Tm-AgB was determined by bioinformatical analyses. The serological diagnostic potentials of rTm-AgB and rTm-TPx were evaluated by indirect ELISA and compared with those of previously reported rTm-AnxB2, rTm-HSP70, and rTm-GST. The results showed that Tm-AgB is a specific lipoprotein of cestodes with good thermal stability. The ELISA assay showed that rTm-AgB exhibited a sensitivity of 95.8% and a specificity of 87.5%, indicating its strong potential for serological diagnosis of T. Multiceps.

  • Molecular characterisation and expression analysis of two heat-shock proteins in Taenia Multiceps.
    Parasites & vectors, 2019
    Co-Authors: Yuchen Liu, Weimin Lai, Yue Xie, Cheng Guo, Xiaowei Dong, Peng Xuerong, Guangyou Yang
    Abstract:

    Taenia Multiceps is a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular chaperones and can affect pathogenicity. Herein, cDNAs of T. Multiceps genes Tm-HSP60 and Tm-p36 were cloned and molecularly characterised by bioinformatics analyses. The immunogenicity and immunoreactivity of recombinant rTm-HSP60 and rTm-p36 proteins were investigated by immunoblotting and indirect ELISA was established to evaluate their serodiagnostic potential. Tissue localisation and transcriptional level at different life stages of T. Multiceps were determined by immunohistochemical and quantitative real-time PCR analyses. The 533 residue rTm-HSP60 and the 314 residue rTm-p36 proteins share typical highly conserved features of HSPs. Tm-p36 shares structural characteristics with metazoan small HSPs, with two N-terminal α-crystallin domains. Compared with Tm-p36, Tm-HSP60 displayed stronger immunogenicity, and the indirect ELISA based on rTm-HSP60 exhibited a sensitivity of 83.3% and a specificity of 87.5%, while rTm-p36 was not suitable to develop indirect ELISA. Tm-HSP60 was widely distributed in all stages of T. Multiceps, albeit at relatively low levels, while Tm-p36 was specifically distributed in the protoscolex and oncosphere. The sequence, structural and functional analyses of these two HSPs indicates that they may play important roles in the life-cycle of T. Multiceps as molecular chaperones. Tm-HSP60 displayed stronger immunogenicity compare to Tm-p36, and has the potential for antibody detection. Tm-p36 was strongly associated with the activation of oncospheres and has potential interest for vaccination.

  • Molecular characterisation and expression analysis of two heat-shock proteins in Taenia Multiceps
    BMC, 2019
    Co-Authors: Yuchen Liu, Weimin Lai, Xuerong Peng, Yue Xie, Cheng Guo, Xiaowei Dong, Guangyou Yang
    Abstract:

    Abstract Background Taenia Multiceps is a harmful tapeworm and its larval form (coenurus cerebralis) is the causative agent of coenurosis, a disease affecting the health of herbivores, resulting in great economic loss to animal husbandry. Heat-shock proteins (HSPs), expressed in all prokaryotes and eukaryotes, act as molecular chaperones and can affect pathogenicity. Methods Herein, cDNAs of T. Multiceps genes Tm-HSP60 and Tm-p36 were cloned and molecularly characterised by bioinformatics analyses. The immunogenicity and immunoreactivity of recombinant rTm-HSP60 and rTm-p36 proteins were investigated by immunoblotting and indirect ELISA was established to evaluate their serodiagnostic potential. Tissue localisation and transcriptional level at different life stages of T. Multiceps were determined by immunohistochemical and quantitative real-time PCR analyses. Result The 533 residue rTm-HSP60 and the 314 residue rTm-p36 proteins share typical highly conserved features of HSPs. Tm-p36 shares structural characteristics with metazoan small HSPs, with two N-terminal α-crystallin domains. Compared with Tm-p36, Tm-HSP60 displayed stronger immunogenicity, and the indirect ELISA based on rTm-HSP60 exhibited a sensitivity of 83.3% and a specificity of 87.5%, while rTm-p36 was not suitable to develop indirect ELISA. Tm-HSP60 was widely distributed in all stages of T. Multiceps, albeit at relatively low levels, while Tm-p36 was specifically distributed in the protoscolex and oncosphere. Conclusions The sequence, structural and functional analyses of these two HSPs indicates that they may play important roles in the life-cycle of T. Multiceps as molecular chaperones. Tm-HSP60 displayed stronger immunogenicity compare to Tm-p36, and has the potential for antibody detection. Tm-p36 was strongly associated with the activation of oncospheres and has potential interest for vaccination

  • Characterization of glutathione S-transferase and its immunodiagnostic potential for detecting Taenia Multiceps.
    Veterinary parasitology, 2017
    Co-Authors: Ying Sun, Xing Huang, Yu Wang, Weimin Lai, Xuerong Peng, Guangyou Yang
    Abstract:

    Abstract Taenia Multiceps is a widespread zoonotic tapeworm parasite which infects cloven-hoofed animals around the world. Animal infection with Coenurus cerebralis, the coenurus larvae of T. Multiceps (Tm), is often fatal, which is a major cause of economic losses in stockbreeding. This study amplified the glutathione S-transferase (GST) gene from the total RNA of C. cerebralis. The resulting protein, Tm-GST, consisted of 201 amino acids, and had a predicted molecular mass of 23.1 kDa. Its amino acid sequence shares 77.61% similarity with Echinococcus granulosus GST. Recombinant Tm-GST (rTm-GST) was expressed in Escherichia coli. The protein reacted with serum from goats infected with T. Multiceps. Immunofluorescence signals indicated that Tm-GST was largely localized in the parenchymatous area of adult T. Multiceps; in addition, it was also apparent in the coenurus. An enzyme-linked immunosorbent assay based on rTm-GST showed specificity of 92.8% (13/14) and sensitivity of 90% (18/20) in detecting anti-GST antibodies in serum from naturally infected animals. This study suggests that Tm-GST has the potential to be used as a diagnostic antigen for Coenurosis.

  • GP50 as a promising early diagnostic antigen for Taenia Multiceps infection in goats by indirect ELISA.
    Parasites & vectors, 2016
    Co-Authors: Xing Huang, Lin Chen, Yu Wang, Weimin Lai, Xuerong Peng, Cheng Guo, Guangyou Yang
    Abstract:

    Background Coenurosis is caused by coenurus, the metacestode of Taenia Multiceps, which mainly parasitizes the brain and spinal cord of cattle, sheep and goats. To date, no widely-approved methods are available to identify early coenurus infection.

Anna Paola Pipia - One of the best experts on this subject based on the ideXlab platform.

  • RESEARCH Open Access Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Omnia Mohamed K, Maria Lucia Manunta, Antonio Scala
    Abstract:

    Background: Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. Methods: The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculate

  • Morphology and genetic variability within Taenia Multiceps in ruminants from Italy.
    Veterinary parasitology, 2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Anna Paola Pipia, Antonio Scala, Giorgia Dessì, A. Zidda, Marco Pau, Belgees Boufana
    Abstract:

    The aim of this study was to investigate the genetic variability and population structure of Taenia Multiceps, and to correlate morphological features of individual coenuri with haplotypes. A total of 92 animals (86 sheep; 4 goats; 1 cattle; 1 mouflon, Ovis musimon) aged between 6-36 months showing clinical symptoms of cerebral coenurosis were included in this study. T. Multiceps coenuri (n=118) sampled from live animals during routine surgery procedures or at post-mortem inspections were examined morphologically and molecularly identified. Morphological features of the 52 coenuri selected for this study (number and size of large and small hooks) were within the range reported in the literature. Fifty-two of the molecularly confirmed T. Multiceps coenuri harboured by 47 animals (sheep=41; cattle=1; goats=4; mouflon=1) were used to determine gene genealogies and population genetic indices and were compared to the 3 T. Multiceps genetic variants, Tm1-Tm3 previously described from Sardinia, Italy. For the 379 bp cox1 dataset we identified 11 polymorphic sites of which 8 were parsimony informative. A high haplotype diversity (0.664±0.067) was recorded for the cox1 sequences defining 10 haplotypes (TM01-TM10). The comparison of haplotypes generated in this study with published T. Multiceps Tm1 variant pointed to the possible existence of a common lineage for T. Multiceps. No correlation was detected between the size of the small and large hooks and the cox 1 haplotypes. Polycystic infestation (2-9 coenuri) was recorded in 27.7% of animals (13/47). No statistical correlation between polycystic T. Multiceps infection and haplotypes was detected.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    Parasites & Vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Background Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps , due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox ( Vulpes vulpes ) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox ( Vulpes vulpes ) in the epidemiology of T. Multiceps and related metacestodoses. Methods The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps . Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. Results A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps . Morphometrical features of the cysts were consistent with those of T. Multiceps ; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox 1 and Nd1) from the metacestode material confirmed the identification. Conclusions The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps
    Parasites & vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps. Morphometrical features of the cysts were consistent with those of T. Multiceps; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox1 and Nd1) from the metacestode material confirmed the identification. The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

  • Preliminary field trial of a vaccine against coenurosis caused by Taenia Multiceps
    Veterinary Parasitology, 2009
    Co-Authors: Antonio Varcasia, Anna Paola Pipia, A. Scala, G Tosciri, G. N. Sanna Coccone, Giovanni Garippa, V. Damien, Gülay Vural, Charles G. Gauci, Marshall W. Lightowlers
    Abstract:

    Abstract Taenia Multiceps is a taeniid cestode that in its adult stage lives in the small intestine of dogs and other canids. In the intermediate hosts, the larval stage of T. Multiceps causes coenurosis, a common disease in the CNS of ruminants, which typically leads to the death of the infected animals. Recent research into new methods for control of coenurosis and other taeniid cestode infections such as hydatidosis has identified vaccination as a potentially valuable new tool. In order to test the applicability of vaccination as an approach for control of T. Multiceps infection in sheep, a field trial was carried out against natural infection in Sardinian farms (Italy) with recombinant proteins of T. Multiceps. The recombinant proteins with Quil A as adjuvant were injected subcutaneously, the first administered to lambs at 10–12 weeks of age and a booster dose given after 2–4 weeks. A total of 632 sheep were selected, belonging to the “replacement quota” of six different farms, of which 424 were used as controls (unvaccinated) and 208 were vaccinated. After a period of more than 40 months from the beginning of the field trial, 33 episodes of cerebral coenurosis occurred in the monitored farms, including 32 cases in control sheep and l case in a vaccinated animal. Statistical analysis revealed a significant reduction in the number of coenurosis cases in the vaccinated animals (χ2 = 14.08, P

Claudia Tamponi - One of the best experts on this subject based on the ideXlab platform.

  • Atypical outbreak of acute coenurosis by Taenia Multiceps in a sheep flock
    Parasitology Research, 2018
    Co-Authors: Davide Pintus, Antonio Varcasia, Claudia Tamponi, Maria Lucia Manunta, Giorgia Dessì, Giovanni Antonio Carboni, Maria Giovanna Cancedda, Ciriaco Ligios, Antonio Scala
    Abstract:

    Herein, we examined the brain of adult ewes and lambs less than 30 days old which were found affected by neurological signs in a flock located in Sardinia (Italy). Gross anatomo-pathological examination of all brains of the animals revealed multiple linear reddish-yellow foci of necrotic purulent inflammation due to oncosphere migration. Histologically, we confirmed a multifocal pyo-granulomatous meningo-encephalitis both in ewes and in lambs, confirming acute coenurosis. Morphological examination and DNA sequencing identified the Taenia Multiceps we isolated as Tm1 strain. This report describes for the first time a natural acute coenurosis infection in suckling lambs under 30 days of age.

  • RESEARCH Open Access Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Omnia Mohamed K, Maria Lucia Manunta, Antonio Scala
    Abstract:

    Background: Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. Methods: The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculate

  • Morphology and genetic variability within Taenia Multiceps in ruminants from Italy.
    Veterinary parasitology, 2016
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Anna Paola Pipia, Antonio Scala, Giorgia Dessì, A. Zidda, Marco Pau, Belgees Boufana
    Abstract:

    The aim of this study was to investigate the genetic variability and population structure of Taenia Multiceps, and to correlate morphological features of individual coenuri with haplotypes. A total of 92 animals (86 sheep; 4 goats; 1 cattle; 1 mouflon, Ovis musimon) aged between 6-36 months showing clinical symptoms of cerebral coenurosis were included in this study. T. Multiceps coenuri (n=118) sampled from live animals during routine surgery procedures or at post-mortem inspections were examined morphologically and molecularly identified. Morphological features of the 52 coenuri selected for this study (number and size of large and small hooks) were within the range reported in the literature. Fifty-two of the molecularly confirmed T. Multiceps coenuri harboured by 47 animals (sheep=41; cattle=1; goats=4; mouflon=1) were used to determine gene genealogies and population genetic indices and were compared to the 3 T. Multiceps genetic variants, Tm1-Tm3 previously described from Sardinia, Italy. For the 379 bp cox1 dataset we identified 11 polymorphic sites of which 8 were parsimony informative. A high haplotype diversity (0.664±0.067) was recorded for the cox1 sequences defining 10 haplotypes (TM01-TM10). The comparison of haplotypes generated in this study with published T. Multiceps Tm1 variant pointed to the possible existence of a common lineage for T. Multiceps. No correlation was detected between the size of the small and large hooks and the cox 1 haplotypes. Polycystic infestation (2-9 coenuri) was recorded in 27.7% of animals (13/47). No statistical correlation between polycystic T. Multiceps infection and haplotypes was detected.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps?
    Parasites & Vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Background Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps , due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox ( Vulpes vulpes ) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox ( Vulpes vulpes ) in the epidemiology of T. Multiceps and related metacestodoses. Methods The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps . Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. Results A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps . Morphometrical features of the cysts were consistent with those of T. Multiceps ; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox 1 and Nd1) from the metacestode material confirmed the identification. Conclusions The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

  • Is the red fox (Vulpes vulpes) a competent definitive host for Taenia Multiceps
    Parasites & vectors, 2015
    Co-Authors: Antonio Varcasia, Claudia Tamponi, Gabriele Tosciri, Anna Paola Pipia, Francesco Dore, Rolf Karl Schuster, Maria Lucia Manunta, Omnia Mohamed Kandil, Antonio Scala
    Abstract:

    Shepherd and stray dogs are thought to represent the primary definitive hosts of Coenurosis by Taenia Multiceps, due to their feeding habits which translate into high chances of coming into contact with infected intermediate hosts. Nonetheless, little attention has been paid to the role of the red fox (Vulpes vulpes) in the epidemiology of coenurosis. In fact a knowledge gap exists on the role played by red foxes in the epidemiology of Taenia Multiceps and the capability of this parasite to produce fertile and viable eggs in this wild canid, i.e. on the occurrence of a sylvatic cycle. This study investigates the role of the red fox (Vulpes vulpes) in the epidemiology of T. Multiceps and related metacestodoses. The small intestine of 63 red foxes was macroscopically examined for the presence of cestodes. Adult parasites were identified morphologically as being T. Multiceps. Tapeworm eggs were counted and stored at 4 °C in physiological saline solution prior to experimental infection of four sheep and one goat. Sheep were inoculated orally on Day 0 with 3000 (sheep 1), 5000 (sheep 2 and 3) or 7000 eggs (sheep 4), while the goat was infected with 5000 eggs of T. Multiceps. The animals were followed-up regularly by MRI and underwent surgical treatment between days 180 to day 240 post infection. Collected coenuri were identified using morphological and molecular methods. A total of 6.3 % of red foxes were found infected with T. Multiceps and the eggs obtained from the worms were determined to have a viability of 45.4 %. Two of the challenged sheep and the goat developed disease compatible with T. Multiceps. Morphometrical features of the cysts were consistent with those of T. Multiceps; nucleotide amplification and sequencing of mitochondrial genes (i.e., cox1 and Nd1) from the metacestode material confirmed the identification. The present study is the first to provide evidence of the role of the red fox as a competent definitive host for T. Multiceps, thus changing the epidemiological scenarios of infections by this cestode.

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