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John E. Moore - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of the sequences of the 16S-23S rDNA
2016Co-Authors: A. Tazumi, Motoo Matsuda, Tsuyoshi Sekizuka, John E. Moore, Cherie B. Millar, Shinji Ono, Cherie MillarAbstract:internal spacer region (ISR) from isolates of Taylorella asinigenitali
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Irish Veterinary Journal
2013Co-Authors: Thomas C. Buckley, Motoo Matsuda, Claire L. Egan, Paula Gibson, Hazel Cosgrove, Siobhan Stanbridge, Cherie B. Millar, John E. MooreAbstract:A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horse
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molecular identification and characterization of the intervening sequences ivss within 23s ribosomal rna rrna genes of Taylorella asinigenitalis isolated in france
Research in Veterinary Science, 2012Co-Authors: A. Tazumi, Sandrine Petry, John E. Moore, Beverley C. Millar, K Hayashi, M. MatsudaAbstract:Abstract In the helix 25 region, 32 French Taylorella asinigenitalis isolates carried at least one 23S rRNA gene not containing intervening sequences (IVSs). No IVSs in the region were identified in three isolates and the other remaining 29 isolates carried one or more IVSs (UCD-1 T IVS1A, UCD-1 T IVS1B and UK-1IVS1B) described already and two new kinds of IVS ( Ta IVS1C and Ta IVS1D). In the helix 45 region, no T. asinigenitalis isolates not carrying any IVSs were identified. UK-1IVS2B was identified in the region from 26 isolates. Five new kinds of IVSs ( Ta IVS2D, E, F, G and H) occurred in the region in the 13 isolates. Distinctly different tandem repeat units (RS48 and RS32 and RS-A, -B and -C) were evident in both regions, respectively, from the French (n = 32) and American (n = 3) T. asinigenitalis isolates. Thus, several different kinds of tandem repeat units and their combinations in IVSs in both regions within the gene were shown in 32 French isolates.
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Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis
BMC Research Notes, 2009Co-Authors: A. Tazumi, Tsuyoshi Sekizuka, John E. Moore, B. Cherie Millar, Shinji Ono, Motoo MatsudaAbstract:Background Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinigenitalis (UCD-1^T, UK-1 and UK-2). Findings Following TA cloning and sequencing, the three isolates of T. asinigenitalis were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1^T and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA^Ile-tRNA^Ala-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of boxB and boxA were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of T. asinigenitalis . This was also confirmed by Southern hybridization procedure. Conclusion The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for T. asinigenitalis , which may aid in the phylogenetic positioning of T. asinigenitalis within the genus Taylorella , and in the molecular discrimination of T. asinigenitalis .
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molecular characterization of the full length 23s and 5s ribosomal rna rrna genes of Taylorella asinigenitalis
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2007Co-Authors: A. Tazumi, Tsuyoshi Sekizuka, Ohoshi Murayama, John E. Moore, Cherie B. Millar, Satoru Saito, Shinzaburo Takamiya, M. MatsudaAbstract:An approximately 4.2 kbp region encoding 23S and 5S rRNA genes was identified when recombinant plasmid DNAs from two genomic DNA libraries and an inverse PCR product of Taylorella asinigenitalis UK-1 isolate were analyzed. Full-length genes of 23S rRNA (3,225 bp) and 5S rRNA (117 bp) of T. asinigenitalis are described. The present sequence analysis identified a non-coding hypothetically intrinsic transcription terminator region downstream of the 5S rRNA gene. The sequence, however, downstream of the 5S rRNA gene did not show any distal tRNA genes. Surprisingly, an intervening sequence (IVS) of 270 bp in length, including two specific tandem repeat units of 80 bp and one partial unit of 48 bp with unknown functions was identified in the first quarter of the 23S rRNA gene sequence. A second IVS of 70 bp in length was also identified in the central region of the 23S rRNA gene. In addition, by using PCR and sequencing procedures, two T. asinigenitalis isolates, UK-1 and UK-2, carried multiple IVSs in the first quarter and central regions. Moreover, the 23S rRNA fragmentation occurred in the UK-1 isolate. A phylogenetic analysis was first carried out based on the 23S rRNA sequence data from T. asinigenitalis UK-1 and 13 other β-Proteobacteria. This is the first report of IVSs in the 23S rRNA gene from the β-Proteobacteria.
Sandrine Petry - One of the best experts on this subject based on the ideXlab platform.
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Towards European harmonisation of contagious equine metritis diagnosis through interlaboratory trials
The Veterinary record, 2018Co-Authors: Sandrine Petry, Marie-france Breuil, Fabien Duquesne, Claire LaugierAbstract:The performance of culture and PCR methods routinely used to diagnose contagious equine metritis (CEM) was evaluated and compared by two interlaboratory trials involving a total of 24 European laboratories, including 22 National Reference Laboratories for CEM. Samples were swab specimens artificially contaminated with bacteria present in the genital tract of Equidae, some with and some without Taylorella equigenitalis, the causative agent of CEM, and T asinigenitalis, responsible for possible misidentification as T equigenitalis Throughout both interlaboratory trials, PCR performed better in terms of specificity and sensitivity than the culture method, supporting the assertion that PCR should be accepted for CEM diagnosis. However, the culture performance during the second interlaboratory trial was better than during the first one, suggesting that the expertise of participants improved. This reveals the advantage of regular interlaboratory trials to constantly improve the expertise of laboratories. It also shows the need to develop new culture media that are more selective and/or better geared to the metabolism of T equigenitalis in order to improve the bacteriological diagnosis of CEM.
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Contagious equine metritis cases reported in France since 2006
The Veterinary record, 2015Co-Authors: Marie-france Breuil, Fabien Duquesne, Claire Laugier, E. Leperchois, B. Ferry, G. Collin, Sandrine PetryAbstract:CONTAGIOUS equine metritis (CEM) is a sexually transmitted disease of equids caused by Taylorella equigenitalis , a Gram-negative microaerophilic coccobacillus of the Taylorella genus. The acute form of the disease is characterised by mucopurulent vaginal discharge and variable degrees of vaginitis, endometritis and cervicitis, leading to temporary infertility (Timoney 2011). It is assumed that disease dissemination results from the transfer of carrier stallions and mares, but is also linked to artificial breeding (Schulman and others 2013). After its discovery in 1977, CEM spread rapidly worldwide, causing international concern within the breeding horse industry (Matsuda and Moore 2003; Timoney 2011). It became one of the most regulated equine diseases and is a notifiable disease of the World Organisation for Animal Health. Despite this, many countries are still considered to have endemic status for CEM within their non-Thoroughbred populations (Schulman and others 2013), probably due to several factors, for example, the absence or the shortcomings of national monitoring, surveillance and reporting programmes, and lack of knowledge about the ecology of T equigenitalis . In France, CEM was reported every year until February 2012, except in the Thoroughbred population which has been CEM free since 2006. No cases have been reported since then, although an increase in the prevalence of the disease was expected since the national regulations (with the exception of artificial breeding) were replaced in 2006 by a voluntary professional system. In this context, the aim of …
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Short communication: Contagious equine metritis cases reported in France since 2006
Veterinary Record, 2015Co-Authors: Marie-france Breuil, Claire Laugier, Sandrine Petry, E. Leperchois, B. Ferry, Francisco Duquesme García, G. CollinAbstract:CONTAGIOUS equine metritis (CEM) is a sexually transmitted disease of equids caused by Taylorella equigenitalis, a Gram-negative microaerophilic coccobacillus of the Taylorella genus. The acute form of the disease is characterised by mucopurulent vaginal discharge and variable degrees of vaginitis, endometritis and cervicitis, leading to temporary infertility (Timoney 2011). It is assumed that disease dissemination results from the transfer of carrier stallions and mares, but is also linked to artificial breeding (Schulman and others 2013). After its discovery in 1977, CEM spread rapidly worldwide, causing international concern within the breeding horse industry (Matsuda and Moore 2003; Timoney 2011). It became one of the most regulated equine diseases and is a notifiable disease of the World Organisation for Animal Health. Despite this, many countries are still considered to have endemic status for CEM within their non-Thoroughbred populations (Schulman and others 2013), probably due to several factors, for example, the absence or the shortcomings of national monitoring, surveillance and reporting programmes, and lack of knowledge about the ecology of T equigenitalis. In France, CEM was reported every year until February 2012, except in the Thoroughbred population which has been CEM free since 2006. No cases have been reported since then, although an increase in the prevalence of the disease was expected since the national regulations (with the exception of artificial breeding) were replaced in 2006 by a voluntary professional system. In this context, the aim of …
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Draft Genome Sequence of Taylorella equigenitalis Strain MCE529, Isolated from a Belgian Warmblood Horse
Genome announcements, 2014Co-Authors: Laurent Hébert, Fabrice Touzain, Claire De Boisséson, Marie-france Breuil, Fabien Duquesne, Claire Laugier, Yannick Blanchard, Sandrine PetryAbstract:ABSTRACT Taylorella equigenitalis is the causative agent of contagious equine metritis (CEM), a sexually transmitted infection of horses. We herein report the genome sequence of T. equigenitalis strain MCE529, isolated in 2009 from the urethral fossa of a 15-year-old Belgian Warmblood horse in France.
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molecular identification and characterization of the intervening sequences ivss within 23s ribosomal rna rrna genes of Taylorella asinigenitalis isolated in france
Research in Veterinary Science, 2012Co-Authors: A. Tazumi, Sandrine Petry, John E. Moore, Beverley C. Millar, K Hayashi, M. MatsudaAbstract:Abstract In the helix 25 region, 32 French Taylorella asinigenitalis isolates carried at least one 23S rRNA gene not containing intervening sequences (IVSs). No IVSs in the region were identified in three isolates and the other remaining 29 isolates carried one or more IVSs (UCD-1 T IVS1A, UCD-1 T IVS1B and UK-1IVS1B) described already and two new kinds of IVS ( Ta IVS1C and Ta IVS1D). In the helix 45 region, no T. asinigenitalis isolates not carrying any IVSs were identified. UK-1IVS2B was identified in the region from 26 isolates. Five new kinds of IVSs ( Ta IVS2D, E, F, G and H) occurred in the region in the 13 isolates. Distinctly different tandem repeat units (RS48 and RS32 and RS-A, -B and -C) were evident in both regions, respectively, from the French (n = 32) and American (n = 3) T. asinigenitalis isolates. Thus, several different kinds of tandem repeat units and their combinations in IVSs in both regions within the gene were shown in 32 French isolates.
Motoo Matsuda - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of the sequences of the 16S-23S rDNA
2016Co-Authors: A. Tazumi, Motoo Matsuda, Tsuyoshi Sekizuka, John E. Moore, Cherie B. Millar, Shinji Ono, Cherie MillarAbstract:internal spacer region (ISR) from isolates of Taylorella asinigenitali
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Irish Veterinary Journal
2013Co-Authors: Thomas C. Buckley, Motoo Matsuda, Claire L. Egan, Paula Gibson, Hazel Cosgrove, Siobhan Stanbridge, Cherie B. Millar, John E. MooreAbstract:A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horse
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Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis
BMC Research Notes, 2009Co-Authors: A. Tazumi, Tsuyoshi Sekizuka, John E. Moore, B. Cherie Millar, Shinji Ono, Motoo MatsudaAbstract:Background Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinigenitalis (UCD-1^T, UK-1 and UK-2). Findings Following TA cloning and sequencing, the three isolates of T. asinigenitalis were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1^T and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA^Ile-tRNA^Ala-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of boxB and boxA were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of T. asinigenitalis . This was also confirmed by Southern hybridization procedure. Conclusion The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for T. asinigenitalis , which may aid in the phylogenetic positioning of T. asinigenitalis within the genus Taylorella , and in the molecular discrimination of T. asinigenitalis .
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Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis
BMC Veterinary Research, 2006Co-Authors: Motoo Matsuda, A. Tazumi, S. Kagawa, Tsuyoshi Sekizuka, Ohoshi Murayama, J.e. Moore, B. C. MillarAbstract:Background At present, six accessible sequences of 16S rDNA from Taylorella equigenitalis ( T. equigenitalis ) are available, whose sequence differences occur at a few nucleotide positions. Thus it is important to determine these sequences from additional strains in other countries, if possible, in order to clarify any anomalies regarding 16S rDNA sequence heterogeneity. Here, we clone and sequence the approximate full-length 16S rDNA from additional strains of T. equigenitalis isolated in Japan, Australia and France and compare these sequences to the existing published sequences. Results Clarification of any anomalies regarding 16S rDNA sequence heterogeneity of T. equigenitalis was carried out. When cloning, sequencing and comparison of the approximate full-length 16S rDNA from 17 strains of T. equigenitalis isolated in Japan, Australia and France, nucleotide sequence differences were demonstrated at the six loci in the 1,469 nucleotide sequence. Moreover, 12 polymorphic sites occurred among 23 sequences of the 16S rDNA, including the six reference sequences. Conclusion High sequence similarity (99.5% or more) was observed throughout, except from nucleotide positions 138 to 501 where substitutions and deletions were noted.
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A two-step species-specific 16S rRNA PCR assay for the detection of Taylorella equigenitalis in horses
Irish Veterinary Journal, 2005Co-Authors: Thomas C. Buckley, Motoo Matsuda, B. Cherie Millar, Claire L. Egan, Paula Gibson, Hazel Cosgrove, Siobhan Stanbridge, John E. MooreAbstract:A two-step PCR assay was developed for the molecular detection of Taylorella equigenitalis, a Gram-negative genital bacterial pathogen in horses. Two specific oligonucleotide primers (TE16SrRNABCHf [25mer] and TE16SrRNABCHr [29mer]) were designed from multiple alignments of the 16S rRNA gene loci of several closely related taxa, including T. asinigenitalis. Subsequent enhanced surveillance of 250 Thoroughbred animals failed to detect the presence of this organism directly from clinical swabs taken from the genital tract of mares and stallions. Such a molecular approach offers a sensitive and specific alternative to conventional culture techniques, and has the potential to lead to improved diagnosis and subsequent management of horses involved in breeding programmes.
A. Tazumi - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of the sequences of the 16S-23S rDNA
2016Co-Authors: A. Tazumi, Motoo Matsuda, Tsuyoshi Sekizuka, John E. Moore, Cherie B. Millar, Shinji Ono, Cherie MillarAbstract:internal spacer region (ISR) from isolates of Taylorella asinigenitali
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molecular identification and characterization of the intervening sequences ivss within 23s ribosomal rna rrna genes of Taylorella asinigenitalis isolated in france
Research in Veterinary Science, 2012Co-Authors: A. Tazumi, Sandrine Petry, John E. Moore, Beverley C. Millar, K Hayashi, M. MatsudaAbstract:Abstract In the helix 25 region, 32 French Taylorella asinigenitalis isolates carried at least one 23S rRNA gene not containing intervening sequences (IVSs). No IVSs in the region were identified in three isolates and the other remaining 29 isolates carried one or more IVSs (UCD-1 T IVS1A, UCD-1 T IVS1B and UK-1IVS1B) described already and two new kinds of IVS ( Ta IVS1C and Ta IVS1D). In the helix 45 region, no T. asinigenitalis isolates not carrying any IVSs were identified. UK-1IVS2B was identified in the region from 26 isolates. Five new kinds of IVSs ( Ta IVS2D, E, F, G and H) occurred in the region in the 13 isolates. Distinctly different tandem repeat units (RS48 and RS32 and RS-A, -B and -C) were evident in both regions, respectively, from the French (n = 32) and American (n = 3) T. asinigenitalis isolates. Thus, several different kinds of tandem repeat units and their combinations in IVSs in both regions within the gene were shown in 32 French isolates.
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Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis
BMC Research Notes, 2009Co-Authors: A. Tazumi, Tsuyoshi Sekizuka, John E. Moore, B. Cherie Millar, Shinji Ono, Motoo MatsudaAbstract:Background Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of Taylorella asinigenitalis (UCD-1^T, UK-1 and UK-2). Findings Following TA cloning and sequencing, the three isolates of T. asinigenitalis were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1^T and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA^Ile-tRNA^Ala-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of boxB and boxA were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of T. asinigenitalis . This was also confirmed by Southern hybridization procedure. Conclusion The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for T. asinigenitalis , which may aid in the phylogenetic positioning of T. asinigenitalis within the genus Taylorella , and in the molecular discrimination of T. asinigenitalis .
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molecular characterization of the full length 23s and 5s ribosomal rna rrna genes of Taylorella asinigenitalis
Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, 2007Co-Authors: A. Tazumi, Tsuyoshi Sekizuka, Ohoshi Murayama, John E. Moore, Cherie B. Millar, Satoru Saito, Shinzaburo Takamiya, M. MatsudaAbstract:An approximately 4.2 kbp region encoding 23S and 5S rRNA genes was identified when recombinant plasmid DNAs from two genomic DNA libraries and an inverse PCR product of Taylorella asinigenitalis UK-1 isolate were analyzed. Full-length genes of 23S rRNA (3,225 bp) and 5S rRNA (117 bp) of T. asinigenitalis are described. The present sequence analysis identified a non-coding hypothetically intrinsic transcription terminator region downstream of the 5S rRNA gene. The sequence, however, downstream of the 5S rRNA gene did not show any distal tRNA genes. Surprisingly, an intervening sequence (IVS) of 270 bp in length, including two specific tandem repeat units of 80 bp and one partial unit of 48 bp with unknown functions was identified in the first quarter of the 23S rRNA gene sequence. A second IVS of 70 bp in length was also identified in the central region of the 23S rRNA gene. In addition, by using PCR and sequencing procedures, two T. asinigenitalis isolates, UK-1 and UK-2, carried multiple IVSs in the first quarter and central regions. Moreover, the 23S rRNA fragmentation occurred in the UK-1 isolate. A phylogenetic analysis was first carried out based on the 23S rRNA sequence data from T. asinigenitalis UK-1 and 13 other β-Proteobacteria. This is the first report of IVSs in the 23S rRNA gene from the β-Proteobacteria.
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Nucleotide Sequencing and Analysis of 16S rDNA and 16S-23S rDNA Internal Spacer Region (ISR) of Taylorella equigenitalis, as an Important Pathogen for Contagious Equine Metritis (CEM)
Veterinary Research Communications, 2006Co-Authors: S. Kagawa, A. Tazumi, B. C. Millar, J.e. Moore, O. Murayama, Y. Nagano, M. MatsudaAbstract:The primer set for 16S rDNA amplified an amplicon of about 1500 bp in length for three strains of Taylorella equigenitalis (NCTC11184^T, Kentucky188 and EQ59). Sequence differences of the 16S rDNA among the six sequences, including three reference sequences, occurred at only a few nucleotide positions and thus, an extremely high sequence similarity of the 16S rDNA was first demonstrated among the six sequences. In addition, the primer set for 16S-23S rDNA internal spacer region (ISR) amplified two amplicons about 1300 bp and 1200 bp in length for the three strains. The ISRs were estimated to be about 920 bp in length for large ISR-A and about 830 bp for small ISR-B. Sequence alignment of the ISR-A and ISR-B demonstrated about 10 base differences between NCTC11184^T and EQ59 and between Kentucky188 and EQ59. However, only minor sequence differences were demonstrated between the ISR-A and ISR-B from NCTC11184^T and Kentucky188, respectively. A typical order of the intercistronic tRNAs with the 29 nucleotide spacer of 5'-16S rDNA-tRNA^Ile-tRNA^Ala-23S rDNA-3' was demonstrated in the all ISRs. The ISRs may be useful for the discrimination amongst isolates of T. equigenitalis if sequencing is employed.
Fabien Duquesne - One of the best experts on this subject based on the ideXlab platform.
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Overview of spatio-temporal distribution inferred by multi-locus sequence typing of Taylorella equigenitalis isolated worldwide from 1977 to 2018 in equidae.
Veterinary microbiology, 2020Co-Authors: Fabien Duquesne, Falk Melzer, Marie-france Breuil, Aurélie Merlin, Iratxe Pérez-cobo, Kamil Sedlák, Gudrun Overesch, David Fretin, Wojciech Iwaniak, Ulrich WerneryAbstract:Abstract The accurate identification of Taylorella equigenitalis strains is essential to improve worldwide prevention and control strategies for contagious equine metritis (CEM). This study compared 367 worldwide equine strains using multilocus sequence typing according to the geographical origin, isolation year and equine breed. The strains were divided into 49 sequence types (STs), including 10 described for the first time. Three major and three minor clonal complexes (CCs), and 11 singletons, were identified. The genetic heterogeneity was low (0.13 STs/strain) despite the wide diversity of geographical origins (n = 16), isolation years (1977 to 2018) and equine breeds (n = 18). It was highest outside Europe and in the 1977-1997 period; current major STs and CCs already existed before 1998. Previous data associated the major CC1 with the first CEM outbreaks in 1977-1978 in the United Kingdom, Australia and the United States, and revealed its circulation in France. Our study confirms its circulation in France over a longer period of time (1992-2018) and its distribution in Spain and Germany but not throughout Europe. In addition to CC1, relationships between non-European and European countries were observed only through ST4, ST17 and ST30. Within Europe, several STs emerged with cross-border circulation, in particular ST16 and ST46 from the major complexes CC2 and CC8. These results constitute a baseline for monitoring the spread of CEM outbreaks. A retrospective analysis of a higher number of strains isolated worldwide between 1977 and the early 2000s would be helpful to obtain an exhaustive picture of the original CEM situation.
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Towards European harmonisation of contagious equine metritis diagnosis through interlaboratory trials
The Veterinary record, 2018Co-Authors: Sandrine Petry, Marie-france Breuil, Fabien Duquesne, Claire LaugierAbstract:The performance of culture and PCR methods routinely used to diagnose contagious equine metritis (CEM) was evaluated and compared by two interlaboratory trials involving a total of 24 European laboratories, including 22 National Reference Laboratories for CEM. Samples were swab specimens artificially contaminated with bacteria present in the genital tract of Equidae, some with and some without Taylorella equigenitalis, the causative agent of CEM, and T asinigenitalis, responsible for possible misidentification as T equigenitalis Throughout both interlaboratory trials, PCR performed better in terms of specificity and sensitivity than the culture method, supporting the assertion that PCR should be accepted for CEM diagnosis. However, the culture performance during the second interlaboratory trial was better than during the first one, suggesting that the expertise of participants improved. This reveals the advantage of regular interlaboratory trials to constantly improve the expertise of laboratories. It also shows the need to develop new culture media that are more selective and/or better geared to the metabolism of T equigenitalis in order to improve the bacteriological diagnosis of CEM.
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Contagious equine metritis cases reported in France since 2006
The Veterinary record, 2015Co-Authors: Marie-france Breuil, Fabien Duquesne, Claire Laugier, E. Leperchois, B. Ferry, G. Collin, Sandrine PetryAbstract:CONTAGIOUS equine metritis (CEM) is a sexually transmitted disease of equids caused by Taylorella equigenitalis , a Gram-negative microaerophilic coccobacillus of the Taylorella genus. The acute form of the disease is characterised by mucopurulent vaginal discharge and variable degrees of vaginitis, endometritis and cervicitis, leading to temporary infertility (Timoney 2011). It is assumed that disease dissemination results from the transfer of carrier stallions and mares, but is also linked to artificial breeding (Schulman and others 2013). After its discovery in 1977, CEM spread rapidly worldwide, causing international concern within the breeding horse industry (Matsuda and Moore 2003; Timoney 2011). It became one of the most regulated equine diseases and is a notifiable disease of the World Organisation for Animal Health. Despite this, many countries are still considered to have endemic status for CEM within their non-Thoroughbred populations (Schulman and others 2013), probably due to several factors, for example, the absence or the shortcomings of national monitoring, surveillance and reporting programmes, and lack of knowledge about the ecology of T equigenitalis . In France, CEM was reported every year until February 2012, except in the Thoroughbred population which has been CEM free since 2006. No cases have been reported since then, although an increase in the prevalence of the disease was expected since the national regulations (with the exception of artificial breeding) were replaced in 2006 by a voluntary professional system. In this context, the aim of …
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Draft Genome Sequence of Taylorella equigenitalis Strain MCE529, Isolated from a Belgian Warmblood Horse
Genome announcements, 2014Co-Authors: Laurent Hébert, Fabrice Touzain, Claire De Boisséson, Marie-france Breuil, Fabien Duquesne, Claire Laugier, Yannick Blanchard, Sandrine PetryAbstract:ABSTRACT Taylorella equigenitalis is the causative agent of contagious equine metritis (CEM), a sexually transmitted infection of horses. We herein report the genome sequence of T. equigenitalis strain MCE529, isolated in 2009 from the urethral fossa of a 15-year-old Belgian Warmblood horse in France.
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Genomic Characterization of the Taylorella Genus
2013Co-Authors: Laurent Hébert, Fabien Duquesne, Bouziane Moumen, Nicolas Pons, Marie-france BreuilAbstract:The Taylorella genus comprises two species: Taylorella equigenitalis, which causes contagious equine metritis, and Taylorella asinigenitalis, a closely-related species mainly found in donkeys. We herein report on the first genome sequence of T. asinigenitalis, analyzing and comparing it with the recently-sequenced T. equigenitalis genome. The T. asinigenitalis genome contains a single circular chromosome of 1,638,559 bp with a 38.3 % GC content and 1,534 coding sequences (CDS). While 212 CDSs were T. asinigenitalis-specific, 1,322 had orthologs in T. equigenitalis. Two hundred and thirty-four T. equigenitalis CDSs had no orthologs in T. asinigenitalis. Analysis of the basic nutrition metabolism of both Taylorella species showed that malate, glutamate and alpha-ketoglutarate may be their main carbon and energy sources. For both species, we identified four different secretion systems and several proteins potentially involved in binding and colonization of host cells, suggesting a strong potential for interaction with their host. T. equigenitalis seems better-equipped than T. asinigenitalis in terms of virulence since we identified numerous proteins potentially involved in pathogenicity, including hemagluttininrelated proteins, a type IV secretion system, TonB-dependent lactoferrin and transferrin receptors, and YadA and Hep_Hag domains containing proteins. This is the first molecular characterization of Taylorella genus members, and the first molecular identification of factors potentially involved in T. asinigenitalis and T. equigenitalis pathogenicity and host colonization. This study facilitates a genetic understanding of growth phenotypes, animal host preference and pathogenic capacity, pavin
