The Experts below are selected from a list of 93 Experts worldwide ranked by ideXlab platform
Yukihiro Matsumoto - One of the best experts on this subject based on the ideXlab platform.
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effect of controlled adverse chamber environment exposure on Tear functions in silicon hydrogel and hydrogel soft contact lens wearers
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Takashi Kojima, Tais Hitomi Wakamatsu, Osama M A Ibrahim, Yukihiro Matsumoto, Miki Uchino, Kazumi Fukagawa, Junko OgawaAbstract:Purpose To prospectively evaluate the effect of controlled adverse chamber environment (CACE) exposure on Tear function, including Tear osmolarity, in subjects wearing narafilcon A versus those wearing etafilcon A soft contact lens (SCL). Methods Thirty-one healthy subjects with no history of contact lens wear (13 women, 18 men; average age, 30.5 ± 6.5 years) were randomly divided into age- and sex-matched groups (15 subjects wearing narafilcon A SCL; 16 subjects wearing etafilcon A SCL) and entered a CACE for 20 minutes. All subjects underwent Tear osmolarity, Tear evaporation rate, strip meniscometry, Tear film breakup time, fluorescein vital staining, and functional visual acuity measurement before and after exposure to the controlled adverse chamber. Results The mean blink rate increased with significant deteriorations in the mean symptom VAS scores, mean Tear osmolarity, Tear evaporation rate, strip meniscometry score, and Tear stability with CACE exposure along with a decrease in visual maintenance ratio in functional visual acuity testing in etafilcon A wearers. The mean symptom VAS scores, mean Tear evaporation rate, Tear stability, blink rates, and visual maintenance ratios did not change significantly in narafilcon A wearers after CACE exposure. Conclusions This study suggested marked Tear Instability, higher Tear osmolarity, and increased Tear evaporation with marked dry eye and visual symptomatology in nonadapted hydrogel SCL wearers, suggesting that silicone hydrogel SCLs may be suitable for persons who live and work in cool, low-humidity, and windy environments, as tested in this study.
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the effects of 2 week senofilcon a silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact Lens and Anterior Eye, 2011Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Takashi Kojima, Yukihiro MatsumotoAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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The effects of 2 week senofilcon—A silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact lens & anterior eye : the journal of the British Contact Lens Association, 2010Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Yukihiro Matsumoto, Takashi Kojima, Junko Ogawa, Jun Shimazaki, Kazuo TsubotaAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease
Molecular Vision, 2010Co-Authors: Tais Hitomi Wakamatsu, Osama M A Ibrahim, Murat Dogru, Yukihiro Matsumoto, Kazumi Fukagawa, Igarashi Ayako, Yoji Takano, Naoko Okada, Yoshiyuki Satake, Jun ShimazakiAbstract:Methods: Twenty eight eyes of 14 patients (9 males, 5 females) with AKC and 18 eyes of 9 age and sex matched (4 males and 5 females) normal healthy controls were examined in this prospective study. The severity of atopic dermatitis (AD) was scored by the SCORing Atopic Dermatitis (SCORAD) index. All subjects underwent Schirmer test, Tear film break up time (BUT), fluorescein/Rose Bengal stainings, Tear collection, and brush cytology from the upper palpebral conjunctiva. The brush cytology samples were stained with Diff-Quik for differentiation of inflammatory cells and immunohistochemistry (IHC) staining with HEL (hexanoyl-lysine) and 4-HNE (4-hydroxy-2-nonenal) to study lipid oxidation. HEL and cytokine (interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), tumor necrosis factoralpha (TNF-α), interferon-gamma (IFN-γ)) levels were measured by enzyme-linked immunosorbent assay (ELISA) from Tear samples of AKC patients and control subjects. Toluidine Blue and IHC staining with HEL, 4-HNE and cluster of differentiation 45 (CD45) were performed on papillary samples of AKC patients. This study was conducted in compliance with the “Declaration of Helsinki.” Results: The Tear stability and vital staining scores were significantly worse in eyes of AKC patients (p<0.05) compared to the controls. Inflammatory cells and positively stained conjunctival epithelial cells for HEL and 4-HNE showed a significant elevation in brush cytology samples of AKC patients. Significantly higher levels of HEL and cytokines were detected in Tears of AKC patients compared to controls. Papillary specimens also revealed many CD45 inflammatory cells as well as many cells positively stained with HEL and 4-HNE in IHC. A strong significant linear positive correlation between conjunctival inflammation and epithelial lipid oxidative stress status was observed. Conjunctival lipid oxidative stress also correlated strongly with Tear HEL levels and epithelial damage scores. Conclusions: The ocular surface disease in AKC was characterized by marked Tear Instability, ocular surface epithelial damage, increase in inflammatory infiltrates and presence of increased lipid oxidation.
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the differences of Tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis
Allergy, 2007Co-Authors: Yukihiro Matsumoto, Murat Dogru, Kazuo Tsubota, Kazumi Fukagawa, N Okada, A Igarashi, Hiroshi FujishimaAbstract:Background: The pathogenesis of the ocular surface disease in atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) has not been fully understood. We tried to clarify the differences in the ocular surface status in patients with AKC, VKC, and healthy control subjects. Methods: Twenty-four eyes of 12 AKC patients, 12 eyes of six VKC patients, and 20 eyes of 10 normal control subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, Tear film break-up time (BUT), vital staining of the ocular surface, conjunctival impression and brush cytology. Impression cytology samples underwent periodic acid Schiff staining for goblet cell density, squamous metaplasia grading, and immunohistochemical staining for MUC1, 2, 4, and 5AC. Brush cytology specimens underwent staining for inflammatory cell counting and Real Time PCR for MUC1, 2, 4, and 5AC mRNA expression. Results: The mean BUT, corneal sensitivity, and conjunctival goblet cell density values in AKC patients were significantly lower compared with VKC patients and control subjects. The squamous metaplasia grades in eyes with AKC were significantly higher compared to eyes with VKC and controls. The inflammatory cell response in brush cytology specimens was different between patients with AKC and VKC. Eyes with AKC showed significantly higher MUC1, 2 and 4 and lower MUC5AC mRNA expression compared to eyes with VKC. Conclusions: Differences of the infiltrates, higher level of Tear Instability, lower corneal sensitivity, up-regulation of MUC1, 2, and 4, and down regulation of MUC5AC were important differential features of the ocular surface disease in AKC compared with VKC.
Kazuo Tsubota - One of the best experts on this subject based on the ideXlab platform.
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Discrepancies in Persistent Dry Eye Signs and Symptoms in Bilateral Pseudophakic Patients
MDPI AG, 2019Co-Authors: Akiko Hanyuda, Kazuo Tsubota, Masahiko Ayaki, Kazuno NegishiAbstract:Despite the increased awareness of early prophylaxis and treatment for dry eye disease (DED) during the first few weeks after cataract surgery, the chronic effect of cataract surgery on the risk of ocular surface abnormalities has not been fully explored. This study was to assess the prevalence of DE subjective symptoms and clinical tests according to the cataract surgery. A total of 172 patients who underwent bilateral cataract surgeries at least 5 months before the recruitment date and 1225 controls with no cataracts were evaluated for their subjective DE symptoms (dry sensation, foreign-body sensation, ocular pain, ocular fatigue, sensitivity to bright light, and blurred vision) and ophthalmic parameters (Tear break-up time, keratoconjunctival staining scores, and maximum blinking interval). The presence of subjective DE symptoms was generally inversely associated with cataract surgeries, whereas abnormal clinical tests were more pronounced among postsurgical cataract patients than among controls. Pseudophakic patients showed a 57% increased prevalence of severe keratoconjunctivitis, compared to controls (P = 0.02). In contrast, among subjective DE symptoms, significantly lower odds of sensitivity to bright light were detected among cases than controls; the multivariable-adjusted odds ratio (95% confidence interval) comparing pseudophakic patients with noncataract patients was 0.56 (0.34⁻0.92) (P = 0.02). In conclusion, persistent Tear Instability and corneal epitheliopathy were found even at several months or more after cataract surgery. This study demonstrates the importance of evaluating ocular surface conditions in pseudophakic patients, even if they lack DE symptoms
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relationship of corneal pain sensitivity with dry eye symptoms in dry eye with short Tear break up time
Investigative Ophthalmology & Visual Science, 2016Co-Authors: Minako Kaido, Motoko Kawashima, Reiko Ishida, Kazuo TsubotaAbstract:PURPOSE: The purpose of this prospective comparative study was to investigate corneal sensitivity in subjects with unstable Tear film, with and without dry eye (DE) symptoms. METHODS: Forty-one eyes of 41 volunteers (mean age: 45.1 ± 9.4 years; age range, 23-57 years), with normal Tear function and ocular surface except for Tear stability, were studied. The eyes were divided into two groups depending on the presence or absence of DE symptoms: 21 eyes with DE symptoms (symptomatic group); and 20 eyes without DE symptoms (asymptomatic group). Three types of corneal sensitivity values were measured using a Cochet-Bonnet esthesiometer: the sensitivity for perception of touch (S-touch), the sensitivity for blinking (S-blink), and the sensitivity for pain (S-pain). RESULTS: Mean S-blink and S-pain were significantly higher in the symptomatic group than in the asymptomatic group (P 0.05). CONCLUSIONS: Corneal sensitivity for blinking and pain evoked by increased stimuli was higher in the symptomatic group (subjects with short break-up time DE) compared with subjects who have no DE symptoms despite decreased Tear stability. The presence of both Tear Instability and hyperesthesia, rather than Tear Instability alone, may contribute to DE pathogenesis.
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The effects of 2 week senofilcon—A silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact lens & anterior eye : the journal of the British Contact Lens Association, 2010Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Yukihiro Matsumoto, Takashi Kojima, Junko Ogawa, Jun Shimazaki, Kazuo TsubotaAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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the differences of Tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis
Allergy, 2007Co-Authors: Yukihiro Matsumoto, Murat Dogru, Kazuo Tsubota, Kazumi Fukagawa, N Okada, A Igarashi, Hiroshi FujishimaAbstract:Background: The pathogenesis of the ocular surface disease in atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) has not been fully understood. We tried to clarify the differences in the ocular surface status in patients with AKC, VKC, and healthy control subjects. Methods: Twenty-four eyes of 12 AKC patients, 12 eyes of six VKC patients, and 20 eyes of 10 normal control subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, Tear film break-up time (BUT), vital staining of the ocular surface, conjunctival impression and brush cytology. Impression cytology samples underwent periodic acid Schiff staining for goblet cell density, squamous metaplasia grading, and immunohistochemical staining for MUC1, 2, 4, and 5AC. Brush cytology specimens underwent staining for inflammatory cell counting and Real Time PCR for MUC1, 2, 4, and 5AC mRNA expression. Results: The mean BUT, corneal sensitivity, and conjunctival goblet cell density values in AKC patients were significantly lower compared with VKC patients and control subjects. The squamous metaplasia grades in eyes with AKC were significantly higher compared to eyes with VKC and controls. The inflammatory cell response in brush cytology specimens was different between patients with AKC and VKC. Eyes with AKC showed significantly higher MUC1, 2 and 4 and lower MUC5AC mRNA expression compared to eyes with VKC. Conclusions: Differences of the infiltrates, higher level of Tear Instability, lower corneal sensitivity, up-regulation of MUC1, 2, and 4, and down regulation of MUC5AC were important differential features of the ocular surface disease in AKC compared with VKC.
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Tear film lipid layer alterations in allergic conjunctivitis
Cornea, 2006Co-Authors: Shintaro Suzuki, Eiki Goto, Naoko Asanokato, Yutaka Hara, Hiroshi Fujishima, Murat Dogru, Yukihiro Matsumoto, Kazuo TsubotaAbstract:Purpose: To assess the alterations of the Tear film lipid layer and Tear functions in patients with seasonal allergic conjunctivitis (SAC) and to compare the results with healthy control subjects. Methods: Seventy-eight eyes of 39 consecutive patients diagnosed as SAC (mean age 32.6 years; 11 male, 28 female) as well as 20 eyes of 10 healthy control subjects (mean age 32.5 years; 6 male, 4 female) underwent slit-lamp examinations, Tear film breakup time measurements (BUT), comeal fluorescein stain scoring, Schirmer test, and Tear film lipid layer interferometry. The 2 groups were then compared for the examined parameters. RAST and serum IgE level evaluations were also carried out in the patients to confirm the diagnosis of allergy. Results: The mean BUT was 3.4 ± 1.5 seconds in patients with SAC compared with the mean value of 12.4 ± 2.4 seconds in the controls (P < 0.05). There were no significant differences in relation to Schirmer test values between the 2 groups; 78% of the patients with SAC had grade 3 or above dry eye change in Tear film lipid layer interferometry, whereas none of the controls had an interferometry grade greater than 3 (grade 1-2 normal; grade 3-4 dry eye; grade 5 severe dry eye). Eyes with SAC had significantly higher Tear film lipid layer thickness ranges compared with the control eyes (P < 0.05). Conclusion: SAC was associated with advanced Tear Instability and thickening of the Tear film lipid layer. Evaluation of the Tear film lipid layer thickness might be useful in the assessment of the extent of dry eye disease and the treatment outcomes in patients with allergy.
Murat Dogru - One of the best experts on this subject based on the ideXlab platform.
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the effects of 2 week senofilcon a silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact Lens and Anterior Eye, 2011Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Takashi Kojima, Yukihiro MatsumotoAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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The effects of 2 week senofilcon—A silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact lens & anterior eye : the journal of the British Contact Lens Association, 2010Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Yukihiro Matsumoto, Takashi Kojima, Junko Ogawa, Jun Shimazaki, Kazuo TsubotaAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease
Molecular Vision, 2010Co-Authors: Tais Hitomi Wakamatsu, Osama M A Ibrahim, Murat Dogru, Yukihiro Matsumoto, Kazumi Fukagawa, Igarashi Ayako, Yoji Takano, Naoko Okada, Yoshiyuki Satake, Jun ShimazakiAbstract:Methods: Twenty eight eyes of 14 patients (9 males, 5 females) with AKC and 18 eyes of 9 age and sex matched (4 males and 5 females) normal healthy controls were examined in this prospective study. The severity of atopic dermatitis (AD) was scored by the SCORing Atopic Dermatitis (SCORAD) index. All subjects underwent Schirmer test, Tear film break up time (BUT), fluorescein/Rose Bengal stainings, Tear collection, and brush cytology from the upper palpebral conjunctiva. The brush cytology samples were stained with Diff-Quik for differentiation of inflammatory cells and immunohistochemistry (IHC) staining with HEL (hexanoyl-lysine) and 4-HNE (4-hydroxy-2-nonenal) to study lipid oxidation. HEL and cytokine (interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), tumor necrosis factoralpha (TNF-α), interferon-gamma (IFN-γ)) levels were measured by enzyme-linked immunosorbent assay (ELISA) from Tear samples of AKC patients and control subjects. Toluidine Blue and IHC staining with HEL, 4-HNE and cluster of differentiation 45 (CD45) were performed on papillary samples of AKC patients. This study was conducted in compliance with the “Declaration of Helsinki.” Results: The Tear stability and vital staining scores were significantly worse in eyes of AKC patients (p<0.05) compared to the controls. Inflammatory cells and positively stained conjunctival epithelial cells for HEL and 4-HNE showed a significant elevation in brush cytology samples of AKC patients. Significantly higher levels of HEL and cytokines were detected in Tears of AKC patients compared to controls. Papillary specimens also revealed many CD45 inflammatory cells as well as many cells positively stained with HEL and 4-HNE in IHC. A strong significant linear positive correlation between conjunctival inflammation and epithelial lipid oxidative stress status was observed. Conjunctival lipid oxidative stress also correlated strongly with Tear HEL levels and epithelial damage scores. Conclusions: The ocular surface disease in AKC was characterized by marked Tear Instability, ocular surface epithelial damage, increase in inflammatory infiltrates and presence of increased lipid oxidation.
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the differences of Tear function and ocular surface findings in patients with atopic keratoconjunctivitis and vernal keratoconjunctivitis
Allergy, 2007Co-Authors: Yukihiro Matsumoto, Murat Dogru, Kazuo Tsubota, Kazumi Fukagawa, N Okada, A Igarashi, Hiroshi FujishimaAbstract:Background: The pathogenesis of the ocular surface disease in atopic keratoconjunctivitis (AKC) and vernal keratoconjunctivitis (VKC) has not been fully understood. We tried to clarify the differences in the ocular surface status in patients with AKC, VKC, and healthy control subjects. Methods: Twenty-four eyes of 12 AKC patients, 12 eyes of six VKC patients, and 20 eyes of 10 normal control subjects were studied. The subjects underwent corneal sensitivity measurements, Schirmer test, Tear film break-up time (BUT), vital staining of the ocular surface, conjunctival impression and brush cytology. Impression cytology samples underwent periodic acid Schiff staining for goblet cell density, squamous metaplasia grading, and immunohistochemical staining for MUC1, 2, 4, and 5AC. Brush cytology specimens underwent staining for inflammatory cell counting and Real Time PCR for MUC1, 2, 4, and 5AC mRNA expression. Results: The mean BUT, corneal sensitivity, and conjunctival goblet cell density values in AKC patients were significantly lower compared with VKC patients and control subjects. The squamous metaplasia grades in eyes with AKC were significantly higher compared to eyes with VKC and controls. The inflammatory cell response in brush cytology specimens was different between patients with AKC and VKC. Eyes with AKC showed significantly higher MUC1, 2 and 4 and lower MUC5AC mRNA expression compared to eyes with VKC. Conclusions: Differences of the infiltrates, higher level of Tear Instability, lower corneal sensitivity, up-regulation of MUC1, 2, and 4, and down regulation of MUC5AC were important differential features of the ocular surface disease in AKC compared with VKC.
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Tear film lipid layer alterations in allergic conjunctivitis
Cornea, 2006Co-Authors: Shintaro Suzuki, Eiki Goto, Naoko Asanokato, Yutaka Hara, Hiroshi Fujishima, Murat Dogru, Yukihiro Matsumoto, Kazuo TsubotaAbstract:Purpose: To assess the alterations of the Tear film lipid layer and Tear functions in patients with seasonal allergic conjunctivitis (SAC) and to compare the results with healthy control subjects. Methods: Seventy-eight eyes of 39 consecutive patients diagnosed as SAC (mean age 32.6 years; 11 male, 28 female) as well as 20 eyes of 10 healthy control subjects (mean age 32.5 years; 6 male, 4 female) underwent slit-lamp examinations, Tear film breakup time measurements (BUT), comeal fluorescein stain scoring, Schirmer test, and Tear film lipid layer interferometry. The 2 groups were then compared for the examined parameters. RAST and serum IgE level evaluations were also carried out in the patients to confirm the diagnosis of allergy. Results: The mean BUT was 3.4 ± 1.5 seconds in patients with SAC compared with the mean value of 12.4 ± 2.4 seconds in the controls (P < 0.05). There were no significant differences in relation to Schirmer test values between the 2 groups; 78% of the patients with SAC had grade 3 or above dry eye change in Tear film lipid layer interferometry, whereas none of the controls had an interferometry grade greater than 3 (grade 1-2 normal; grade 3-4 dry eye; grade 5 severe dry eye). Eyes with SAC had significantly higher Tear film lipid layer thickness ranges compared with the control eyes (P < 0.05). Conclusion: SAC was associated with advanced Tear Instability and thickening of the Tear film lipid layer. Evaluation of the Tear film lipid layer thickness might be useful in the assessment of the extent of dry eye disease and the treatment outcomes in patients with allergy.
Tais Hitomi Wakamatsu - One of the best experts on this subject based on the ideXlab platform.
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effect of controlled adverse chamber environment exposure on Tear functions in silicon hydrogel and hydrogel soft contact lens wearers
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Takashi Kojima, Tais Hitomi Wakamatsu, Osama M A Ibrahim, Yukihiro Matsumoto, Miki Uchino, Kazumi Fukagawa, Junko OgawaAbstract:Purpose To prospectively evaluate the effect of controlled adverse chamber environment (CACE) exposure on Tear function, including Tear osmolarity, in subjects wearing narafilcon A versus those wearing etafilcon A soft contact lens (SCL). Methods Thirty-one healthy subjects with no history of contact lens wear (13 women, 18 men; average age, 30.5 ± 6.5 years) were randomly divided into age- and sex-matched groups (15 subjects wearing narafilcon A SCL; 16 subjects wearing etafilcon A SCL) and entered a CACE for 20 minutes. All subjects underwent Tear osmolarity, Tear evaporation rate, strip meniscometry, Tear film breakup time, fluorescein vital staining, and functional visual acuity measurement before and after exposure to the controlled adverse chamber. Results The mean blink rate increased with significant deteriorations in the mean symptom VAS scores, mean Tear osmolarity, Tear evaporation rate, strip meniscometry score, and Tear stability with CACE exposure along with a decrease in visual maintenance ratio in functional visual acuity testing in etafilcon A wearers. The mean symptom VAS scores, mean Tear evaporation rate, Tear stability, blink rates, and visual maintenance ratios did not change significantly in narafilcon A wearers after CACE exposure. Conclusions This study suggested marked Tear Instability, higher Tear osmolarity, and increased Tear evaporation with marked dry eye and visual symptomatology in nonadapted hydrogel SCL wearers, suggesting that silicone hydrogel SCLs may be suitable for persons who live and work in cool, low-humidity, and windy environments, as tested in this study.
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the effects of 2 week senofilcon a silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact Lens and Anterior Eye, 2011Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Takashi Kojima, Yukihiro MatsumotoAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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The effects of 2 week senofilcon—A silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact lens & anterior eye : the journal of the British Contact Lens Association, 2010Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Yukihiro Matsumoto, Takashi Kojima, Junko Ogawa, Jun Shimazaki, Kazuo TsubotaAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease
Molecular Vision, 2010Co-Authors: Tais Hitomi Wakamatsu, Osama M A Ibrahim, Murat Dogru, Yukihiro Matsumoto, Kazumi Fukagawa, Igarashi Ayako, Yoji Takano, Naoko Okada, Yoshiyuki Satake, Jun ShimazakiAbstract:Methods: Twenty eight eyes of 14 patients (9 males, 5 females) with AKC and 18 eyes of 9 age and sex matched (4 males and 5 females) normal healthy controls were examined in this prospective study. The severity of atopic dermatitis (AD) was scored by the SCORing Atopic Dermatitis (SCORAD) index. All subjects underwent Schirmer test, Tear film break up time (BUT), fluorescein/Rose Bengal stainings, Tear collection, and brush cytology from the upper palpebral conjunctiva. The brush cytology samples were stained with Diff-Quik for differentiation of inflammatory cells and immunohistochemistry (IHC) staining with HEL (hexanoyl-lysine) and 4-HNE (4-hydroxy-2-nonenal) to study lipid oxidation. HEL and cytokine (interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), tumor necrosis factoralpha (TNF-α), interferon-gamma (IFN-γ)) levels were measured by enzyme-linked immunosorbent assay (ELISA) from Tear samples of AKC patients and control subjects. Toluidine Blue and IHC staining with HEL, 4-HNE and cluster of differentiation 45 (CD45) were performed on papillary samples of AKC patients. This study was conducted in compliance with the “Declaration of Helsinki.” Results: The Tear stability and vital staining scores were significantly worse in eyes of AKC patients (p<0.05) compared to the controls. Inflammatory cells and positively stained conjunctival epithelial cells for HEL and 4-HNE showed a significant elevation in brush cytology samples of AKC patients. Significantly higher levels of HEL and cytokines were detected in Tears of AKC patients compared to controls. Papillary specimens also revealed many CD45 inflammatory cells as well as many cells positively stained with HEL and 4-HNE in IHC. A strong significant linear positive correlation between conjunctival inflammation and epithelial lipid oxidative stress status was observed. Conjunctival lipid oxidative stress also correlated strongly with Tear HEL levels and epithelial damage scores. Conclusions: The ocular surface disease in AKC was characterized by marked Tear Instability, ocular surface epithelial damage, increase in inflammatory infiltrates and presence of increased lipid oxidation.
Osama M A Ibrahim - One of the best experts on this subject based on the ideXlab platform.
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effect of controlled adverse chamber environment exposure on Tear functions in silicon hydrogel and hydrogel soft contact lens wearers
Investigative Ophthalmology & Visual Science, 2011Co-Authors: Takashi Kojima, Tais Hitomi Wakamatsu, Osama M A Ibrahim, Yukihiro Matsumoto, Miki Uchino, Kazumi Fukagawa, Junko OgawaAbstract:Purpose To prospectively evaluate the effect of controlled adverse chamber environment (CACE) exposure on Tear function, including Tear osmolarity, in subjects wearing narafilcon A versus those wearing etafilcon A soft contact lens (SCL). Methods Thirty-one healthy subjects with no history of contact lens wear (13 women, 18 men; average age, 30.5 ± 6.5 years) were randomly divided into age- and sex-matched groups (15 subjects wearing narafilcon A SCL; 16 subjects wearing etafilcon A SCL) and entered a CACE for 20 minutes. All subjects underwent Tear osmolarity, Tear evaporation rate, strip meniscometry, Tear film breakup time, fluorescein vital staining, and functional visual acuity measurement before and after exposure to the controlled adverse chamber. Results The mean blink rate increased with significant deteriorations in the mean symptom VAS scores, mean Tear osmolarity, Tear evaporation rate, strip meniscometry score, and Tear stability with CACE exposure along with a decrease in visual maintenance ratio in functional visual acuity testing in etafilcon A wearers. The mean symptom VAS scores, mean Tear evaporation rate, Tear stability, blink rates, and visual maintenance ratios did not change significantly in narafilcon A wearers after CACE exposure. Conclusions This study suggested marked Tear Instability, higher Tear osmolarity, and increased Tear evaporation with marked dry eye and visual symptomatology in nonadapted hydrogel SCL wearers, suggesting that silicone hydrogel SCLs may be suitable for persons who live and work in cool, low-humidity, and windy environments, as tested in this study.
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the effects of 2 week senofilcon a silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact Lens and Anterior Eye, 2011Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Takashi Kojima, Yukihiro MatsumotoAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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The effects of 2 week senofilcon—A silicone hydrogel contact lens daily wear on Tear functions and ocular surface health status
Contact lens & anterior eye : the journal of the British Contact Lens Association, 2010Co-Authors: Murat Dogru, Tais Hitomi Wakamatsu, Cristina M Schnider, Samantha K Ward, Osama M A Ibrahim, Yukihiro Matsumoto, Takashi Kojima, Junko Ogawa, Jun Shimazaki, Kazuo TsubotaAbstract:Abstract Purpose To prospectively investigate the effects of 2 week senofilcon A contact lens (CL) daily wear on the functional visual acuity (VA), ocular surface and Tear film. Methods Seventeen right eyes of 17 senofilcon A CL wearers without any ocular or systemic diseases were examined before and 2 weeks after lens wear. Visual acuity measurements, Tear evaporation rate, ELISA for Tear cytokines, strip meniscometry, Tear lipid layer interferometry, Tear film break-up time (BUT), in vivo confocal microscopy, corneal sensitivity, ocular surface vital staining, Schirmer I test and brush cytology for MUC5AC mRNA expression were performed before and after CL wear. Results The best corrected Landolt VA, functional VA parameters, the mean lipid layer interferometry grades, Tear evaporation rates, Schirmer test values, vital staining scores and in vivo confocal microscopy parameters did not show any significant differences after 2 weeks of CL wear. The Tear film BUT showed a significant decrease together with a significant down regulation of MUC5 AC mRNA expression after CL wear. A statistically significant elevation in the mean Tear interleukin (IL)-6 concentration was also observed after 2 weeks of CL wear. Conclusions Two week senofilcon A daily CL wear seems to be associated with Tear Instability, a decrease in MUC5AC expression, and elevation of IL-6 in Tears without significant alterations in epithelial damage scores or in the morphology or density of in vivo keratoconjunctival cells and nerves. Alterations associated with long term wear and patients with dry eye disease need to be studied in future trials.
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evaluation of lipid oxidative stress status and inflammation in atopic ocular surface disease
Molecular Vision, 2010Co-Authors: Tais Hitomi Wakamatsu, Osama M A Ibrahim, Murat Dogru, Yukihiro Matsumoto, Kazumi Fukagawa, Igarashi Ayako, Yoji Takano, Naoko Okada, Yoshiyuki Satake, Jun ShimazakiAbstract:Methods: Twenty eight eyes of 14 patients (9 males, 5 females) with AKC and 18 eyes of 9 age and sex matched (4 males and 5 females) normal healthy controls were examined in this prospective study. The severity of atopic dermatitis (AD) was scored by the SCORing Atopic Dermatitis (SCORAD) index. All subjects underwent Schirmer test, Tear film break up time (BUT), fluorescein/Rose Bengal stainings, Tear collection, and brush cytology from the upper palpebral conjunctiva. The brush cytology samples were stained with Diff-Quik for differentiation of inflammatory cells and immunohistochemistry (IHC) staining with HEL (hexanoyl-lysine) and 4-HNE (4-hydroxy-2-nonenal) to study lipid oxidation. HEL and cytokine (interleukin-4 (IL-4), interleukin-5 (IL-5), interleukin-10 (IL-10), tumor necrosis factoralpha (TNF-α), interferon-gamma (IFN-γ)) levels were measured by enzyme-linked immunosorbent assay (ELISA) from Tear samples of AKC patients and control subjects. Toluidine Blue and IHC staining with HEL, 4-HNE and cluster of differentiation 45 (CD45) were performed on papillary samples of AKC patients. This study was conducted in compliance with the “Declaration of Helsinki.” Results: The Tear stability and vital staining scores were significantly worse in eyes of AKC patients (p<0.05) compared to the controls. Inflammatory cells and positively stained conjunctival epithelial cells for HEL and 4-HNE showed a significant elevation in brush cytology samples of AKC patients. Significantly higher levels of HEL and cytokines were detected in Tears of AKC patients compared to controls. Papillary specimens also revealed many CD45 inflammatory cells as well as many cells positively stained with HEL and 4-HNE in IHC. A strong significant linear positive correlation between conjunctival inflammation and epithelial lipid oxidative stress status was observed. Conjunctival lipid oxidative stress also correlated strongly with Tear HEL levels and epithelial damage scores. Conclusions: The ocular surface disease in AKC was characterized by marked Tear Instability, ocular surface epithelial damage, increase in inflammatory infiltrates and presence of increased lipid oxidation.
