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David K. Gardner - One of the best experts on this subject based on the ideXlab platform.

  • single blastocyst transfer a prospective randomized trial
    Fertility and Sterility, 2004
    Co-Authors: David K. Gardner, Eric S Surrey, D A Minjarez, Annette Leitz
    Abstract:

    Objective To determine the efficacy of single blastocyst transfer. Design Prospective randomized trial. Setting Private assisted reproductive Technology Unit. Patient(s) Forty-eight women undergoing IVF-embryo transfer with day 3 FSH 12 mm in diameter on day of hCG administration. Intervention(s) Embryo culture to the blastocyst stage in sequential media G1/G2 followed by transfer of either one or two blastocysts. Main outcome measure(s) Implantation rate, ongoing pregnancy rate, and twinning. Result(s) The transfer of a single blastocyst resulted in an implantation and ongoing pregnancy rate of 60.9% with no twins. The transfer of two blastocysts resulted in an implantation rate of 56%, an ongoing pregnancy rate of 76% with a 47.4% incidence of twins. Conclusion(s) Single blastocyst transfer is an effective method of eliminating multiple births while maintaining high pregnancy rates in this selected group of patients.

  • blastocyst culture and transfer increases the efficiency of oocyte donation
    Fertility and Sterility, 2000
    Co-Authors: William B. Schoolcraft, David K. Gardner
    Abstract:

    Abstract Objective: To determine the impact of blastocyst transfer on an oocyte donation program. Design: Retrospective review of embryo transfer in an IVF clinic. Setting: Private assisted reproductive Technology Unit. Patient(s): Two hundred and twenty nine patients undergoing oocyte donation. Intervention(s): Culture of pronucleate embryos to either day 3 or day 5 followed by embryo transfer. Main Outcome Measure(s): Implantation rates, pregnancy rates, and multiple gestations were analyzed. Result(s): Implantation rates and pregnancy rates were significantly increased by moving to extended embryo culture and transfer on day 5. After day 3 transfers, implantation and pregnancy rates were 47.1% and 75%, respectively. In contrast, on day 5 these rates were increased to 65.8% and 87.6%. Concomitantly, there were significantly fewer embryos transferred on day 5 (2.1) compared to day 3 (3.2). Conclusion(s): Blastocyst transfer is a highly effective treatment for patients who receive donor oocytes, allowing excellent pregnancy rates while significantly reducing the incidence of high-order multiple gestations.

  • noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
    Fertility and Sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, J M Stevens, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst development and morphology and embryo metabolism. Design: Noninvasive assessment of carbohydrate uptake and ammonium production by individual embryos. Setting: Private assisted reproductive Technology Unit. Patient(s): Patients donated, with consent, cryopreserved pronucleate embryos and noncryopreserved blastocysts. Intervention(s): Culture of 60 thawed pronucleate embryos in sequential media to the blastocyst stage with concomitant noninvasive analysis of embryo metabolism and analysis of 13 blastocysts from noncryopreserved embryos. Main Outcome Measure(s): Pyruvate and glucose consumption as well as blastocyst formation and quality. Result(s): Pyruvate and glucose uptakes on day 4 were significantly higher by embryos that went on to form blastocysts than by embryos that failed to develop to the blastocyst stage. Glucose uptakes were greatest in those blastocysts of highest grade, whereas pyruvate uptakes were similar irrespective of blastocyst grade, indicating that glucose is the more important nutrient for the human blastocyst. Among blastocysts of the same grade from the same patient, there was considerable spread of glucose consumption, indicating that glucose consumption may be of use in identifying blastocysts for transfer. Ammonium production by individual embryos was also measured, reflecting amino acid transamination and use by the human embryo. Conclusion(s): The ability to identify in culture the embryo with the highest developmental potential will facilitate the move to single-embryo transfers.

  • Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
    Fertility and sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, John Stevens, T. Schlenker, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst score and pregnancy outcome. Design: Retrospective review of blastocyst transfer in an IVF clinic. Setting: Private assisted reproductive Technology Unit. Patient(s): 107 patients undergoing blastocyst culture and transfer of two embryos. Intervention(s): Culture of all pronucleate embryos in sequential media to the blastocyst stage (day 5), followed by transfer of two blastocysts. Main Outcome Measure(s): Implantation rates, pregnancy rates, and twinning were analyzed. Result(s): When a patient received two top-scoring blastocysts (64% of patients), implantation and pregnancy rates were 70% and 87%, respectively. The twinning rate in this group was 61%. When only one top-quality blastocyst was available for transfer (21% of patients), the implantation and pregnancy rates were 50% and 70%. The twinning rate for this group was 50%. In contrast, when only low-scoring blastocysts were available for transfer (15% of patients), implantation and pregnancy rates were 28% and 44%, and the twinning rate was 29%. No monozygotic twins were observed in this group of patients. Conclusion(s): The ability to transfer one high-scoring blastocyst should lead to pregnancy rates greater than 60%, without the complication of twins.

William B. Schoolcraft - One of the best experts on this subject based on the ideXlab platform.

  • blastocyst culture and transfer increases the efficiency of oocyte donation
    Fertility and Sterility, 2000
    Co-Authors: William B. Schoolcraft, David K. Gardner
    Abstract:

    Abstract Objective: To determine the impact of blastocyst transfer on an oocyte donation program. Design: Retrospective review of embryo transfer in an IVF clinic. Setting: Private assisted reproductive Technology Unit. Patient(s): Two hundred and twenty nine patients undergoing oocyte donation. Intervention(s): Culture of pronucleate embryos to either day 3 or day 5 followed by embryo transfer. Main Outcome Measure(s): Implantation rates, pregnancy rates, and multiple gestations were analyzed. Result(s): Implantation rates and pregnancy rates were significantly increased by moving to extended embryo culture and transfer on day 5. After day 3 transfers, implantation and pregnancy rates were 47.1% and 75%, respectively. In contrast, on day 5 these rates were increased to 65.8% and 87.6%. Concomitantly, there were significantly fewer embryos transferred on day 5 (2.1) compared to day 3 (3.2). Conclusion(s): Blastocyst transfer is a highly effective treatment for patients who receive donor oocytes, allowing excellent pregnancy rates while significantly reducing the incidence of high-order multiple gestations.

  • noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
    Fertility and Sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, J M Stevens, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst development and morphology and embryo metabolism. Design: Noninvasive assessment of carbohydrate uptake and ammonium production by individual embryos. Setting: Private assisted reproductive Technology Unit. Patient(s): Patients donated, with consent, cryopreserved pronucleate embryos and noncryopreserved blastocysts. Intervention(s): Culture of 60 thawed pronucleate embryos in sequential media to the blastocyst stage with concomitant noninvasive analysis of embryo metabolism and analysis of 13 blastocysts from noncryopreserved embryos. Main Outcome Measure(s): Pyruvate and glucose consumption as well as blastocyst formation and quality. Result(s): Pyruvate and glucose uptakes on day 4 were significantly higher by embryos that went on to form blastocysts than by embryos that failed to develop to the blastocyst stage. Glucose uptakes were greatest in those blastocysts of highest grade, whereas pyruvate uptakes were similar irrespective of blastocyst grade, indicating that glucose is the more important nutrient for the human blastocyst. Among blastocysts of the same grade from the same patient, there was considerable spread of glucose consumption, indicating that glucose consumption may be of use in identifying blastocysts for transfer. Ammonium production by individual embryos was also measured, reflecting amino acid transamination and use by the human embryo. Conclusion(s): The ability to identify in culture the embryo with the highest developmental potential will facilitate the move to single-embryo transfers.

  • Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
    Fertility and sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, John Stevens, T. Schlenker, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst score and pregnancy outcome. Design: Retrospective review of blastocyst transfer in an IVF clinic. Setting: Private assisted reproductive Technology Unit. Patient(s): 107 patients undergoing blastocyst culture and transfer of two embryos. Intervention(s): Culture of all pronucleate embryos in sequential media to the blastocyst stage (day 5), followed by transfer of two blastocysts. Main Outcome Measure(s): Implantation rates, pregnancy rates, and twinning were analyzed. Result(s): When a patient received two top-scoring blastocysts (64% of patients), implantation and pregnancy rates were 70% and 87%, respectively. The twinning rate in this group was 61%. When only one top-quality blastocyst was available for transfer (21% of patients), the implantation and pregnancy rates were 50% and 70%. The twinning rate for this group was 50%. In contrast, when only low-scoring blastocysts were available for transfer (15% of patients), implantation and pregnancy rates were 28% and 44%, and the twinning rate was 29%. No monozygotic twins were observed in this group of patients. Conclusion(s): The ability to transfer one high-scoring blastocyst should lead to pregnancy rates greater than 60%, without the complication of twins.

Annette Leitz - One of the best experts on this subject based on the ideXlab platform.

  • single blastocyst transfer a prospective randomized trial
    Fertility and Sterility, 2004
    Co-Authors: David K. Gardner, Eric S Surrey, D A Minjarez, Annette Leitz
    Abstract:

    Objective To determine the efficacy of single blastocyst transfer. Design Prospective randomized trial. Setting Private assisted reproductive Technology Unit. Patient(s) Forty-eight women undergoing IVF-embryo transfer with day 3 FSH 12 mm in diameter on day of hCG administration. Intervention(s) Embryo culture to the blastocyst stage in sequential media G1/G2 followed by transfer of either one or two blastocysts. Main outcome measure(s) Implantation rate, ongoing pregnancy rate, and twinning. Result(s) The transfer of a single blastocyst resulted in an implantation and ongoing pregnancy rate of 60.9% with no twins. The transfer of two blastocysts resulted in an implantation rate of 56%, an ongoing pregnancy rate of 76% with a 47.4% incidence of twins. Conclusion(s) Single blastocyst transfer is an effective method of eliminating multiple births while maintaining high pregnancy rates in this selected group of patients.

Michelle Lane - One of the best experts on this subject based on the ideXlab platform.

  • noninvasive assessment of human embryo nutrient consumption as a measure of developmental potential
    Fertility and Sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, J M Stevens, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst development and morphology and embryo metabolism. Design: Noninvasive assessment of carbohydrate uptake and ammonium production by individual embryos. Setting: Private assisted reproductive Technology Unit. Patient(s): Patients donated, with consent, cryopreserved pronucleate embryos and noncryopreserved blastocysts. Intervention(s): Culture of 60 thawed pronucleate embryos in sequential media to the blastocyst stage with concomitant noninvasive analysis of embryo metabolism and analysis of 13 blastocysts from noncryopreserved embryos. Main Outcome Measure(s): Pyruvate and glucose consumption as well as blastocyst formation and quality. Result(s): Pyruvate and glucose uptakes on day 4 were significantly higher by embryos that went on to form blastocysts than by embryos that failed to develop to the blastocyst stage. Glucose uptakes were greatest in those blastocysts of highest grade, whereas pyruvate uptakes were similar irrespective of blastocyst grade, indicating that glucose is the more important nutrient for the human blastocyst. Among blastocysts of the same grade from the same patient, there was considerable spread of glucose consumption, indicating that glucose consumption may be of use in identifying blastocysts for transfer. Ammonium production by individual embryos was also measured, reflecting amino acid transamination and use by the human embryo. Conclusion(s): The ability to identify in culture the embryo with the highest developmental potential will facilitate the move to single-embryo transfers.

  • Blastocyst score affects implantation and pregnancy outcome: towards a single blastocyst transfer
    Fertility and sterility, 2000
    Co-Authors: David K. Gardner, Michelle Lane, John Stevens, T. Schlenker, William B. Schoolcraft
    Abstract:

    Abstract Objective: To determine the relationship between blastocyst score and pregnancy outcome. Design: Retrospective review of blastocyst transfer in an IVF clinic. Setting: Private assisted reproductive Technology Unit. Patient(s): 107 patients undergoing blastocyst culture and transfer of two embryos. Intervention(s): Culture of all pronucleate embryos in sequential media to the blastocyst stage (day 5), followed by transfer of two blastocysts. Main Outcome Measure(s): Implantation rates, pregnancy rates, and twinning were analyzed. Result(s): When a patient received two top-scoring blastocysts (64% of patients), implantation and pregnancy rates were 70% and 87%, respectively. The twinning rate in this group was 61%. When only one top-quality blastocyst was available for transfer (21% of patients), the implantation and pregnancy rates were 50% and 70%. The twinning rate for this group was 50%. In contrast, when only low-scoring blastocysts were available for transfer (15% of patients), implantation and pregnancy rates were 28% and 44%, and the twinning rate was 29%. No monozygotic twins were observed in this group of patients. Conclusion(s): The ability to transfer one high-scoring blastocyst should lead to pregnancy rates greater than 60%, without the complication of twins.

D A Minjarez - One of the best experts on this subject based on the ideXlab platform.

  • single blastocyst transfer a prospective randomized trial
    Fertility and Sterility, 2004
    Co-Authors: David K. Gardner, Eric S Surrey, D A Minjarez, Annette Leitz
    Abstract:

    Objective To determine the efficacy of single blastocyst transfer. Design Prospective randomized trial. Setting Private assisted reproductive Technology Unit. Patient(s) Forty-eight women undergoing IVF-embryo transfer with day 3 FSH 12 mm in diameter on day of hCG administration. Intervention(s) Embryo culture to the blastocyst stage in sequential media G1/G2 followed by transfer of either one or two blastocysts. Main outcome measure(s) Implantation rate, ongoing pregnancy rate, and twinning. Result(s) The transfer of a single blastocyst resulted in an implantation and ongoing pregnancy rate of 60.9% with no twins. The transfer of two blastocysts resulted in an implantation rate of 56%, an ongoing pregnancy rate of 76% with a 47.4% incidence of twins. Conclusion(s) Single blastocyst transfer is an effective method of eliminating multiple births while maintaining high pregnancy rates in this selected group of patients.