The Experts below are selected from a list of 168 Experts worldwide ranked by ideXlab platform
Takashi R. Endo - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.
Frontiers in plant science, 2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
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Data_Sheet_1_Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.PDF
2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
Shotaro Takenaka - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.
Frontiers in plant science, 2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
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Data_Sheet_1_Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.PDF
2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
Jan Dvorak - One of the best experts on this subject based on the ideXlab platform.
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comparisons of recombination frequencies in hybrids involving Telocentric and bibrachial wheat Chromosomes
Theoretical and Applied Genetics, 2000Co-Authors: Jorge Dubcovsky, G Tranquilli, I A Khan, L Pfluger, E Y Suarez, M Rousset, Jan DvorakAbstract:Telosomic stocks have been extensively used to map genes to chromosome arms and to determine gene-to-centromere genetic distances. It has been suggested that if a chromosome arm is present as a telosome, recombination frequencies will be drastically reduced in the centromeric region. However, previous studies have not considered the bias in recombination estimates due to selection against aneuploid gametes produced by failure of pairing at the first meiotic division. Formulas are derived here for adjusting recombination estimates for this bias. Adjusted recombination frequencies between markers located on both sides of the centromeres are analyzed in three different pairs of wheat (Triticum aestivum) isogenic segregating populations involving bibrachial and Telocentric Chromosomes. Recombination frequencies estimated from crosses involving Telocentric Chromosomes were not significantly different from recombination frequencies estimated from isogenic crosses involving bibrachial Chromosomes. The implications of the present findings for karyotype evolution, and specifically for Robertsonian fissions and fusions, are discussed.
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comparisons of recombination frequencies in hybrids involving Telocentric and bibrachial wheat Chromosomes escholarship
2000Co-Authors: Jorge Dubcovsky, G Tranquilli, I A Khan, E Y Suarez, M Rousset, L A Pfluger, Jan DvorakAbstract:Telosomic stocks have been extensively used to map genes to chromosome arms and to determine gene-to-centromere genetic distances. It has been suggested that if a chromosome arm is present as a telosome, recombination frequencies will be drastically reduced in the centromeric region. However, previous studies have not considered the bias in recombination estimates due to selection against aneuploid gametes produced by failure of pairing at the first meiotic division. Formulas are derived here for adjusting recombination estimates for this bias. Adjusted recombination frequencies between markers located on both sides of the centromeres are analyzed in three different pairs of wheat (Triticum aestivum) isogenic segregating populations involving bibrachial and Telocentric Chromosomes. Recombination frequencies estimated from crosses involving Telocentric Chromosomes were not significantly different from recombination frequencies estimated from isogenic crosses involving bibrachial Chromosomes. The implications of the present findings for karyotype evolution, and specifically for Robertsonian fissions and fusions, are discussed.
Minoru Murata - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.
Frontiers in plant science, 2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
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Data_Sheet_1_Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.PDF
2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
Bettina Brückner - One of the best experts on this subject based on the ideXlab platform.
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Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.
Frontiers in plant science, 2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
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Data_Sheet_1_Chromosome Arm Locations of Barley Sucrose Transporter Gene in Transgenic Winter Wheat Lines.PDF
2019Co-Authors: Shotaro Takenaka, Winfriede Weschke, Bettina Brückner, Minoru Murata, Takashi R. EndoAbstract:Three transgenic HOSUT lines of winter wheat, HOSUT12, HOSUT20, and HOSUT24, each harbor a single copy of the cDNA for the barley sucrose transporter gene HvSUT1 (SUT), which was fused to the barley endosperm-specific Hordein B1 promoter (HO; the HOSUT transgene). Previously, flow cytometry combined with PCR analysis demonstrated that the HOSUT transgene had been integrated into different wheat Chromosomes: 7A, 5D, and 4A in HOSUT12, HOSUT20, and HOSUT24, respectively. In order to confirm the chromosomal location of the HOSUT transgene by a cytological approach using wheat aneuploid stocks, we crossed corresponding nullisomic-tetrasomic lines with the three HOSUT lines, namely nullisomic 7A-tetrasomic 7B with HOSUT12, nullisomic 5D-tetrasomic 5B with HOSUT20, and nullisomic 4A-tetrasomic 4B with HOSUT24. We examined the resulting chromosomal constitutions and the presence of the HOSUT transgene in the F2 progeny by means of chromosome banding and PCR. The chromosome banding patterns of the critical Chromosomes in the original HOSUT lines showed no difference from those of the corresponding wild type Chromosomes. The presence or absence of the critical Chromosomes completely corresponded to the presence or absence of the HOSUT transgene in the F2 plants. Investigating Telocentric Chromosomes occurred in the F2 progeny, which were derived from the respective critical HOSUT Chromosomes, we found that the HOSUT transgene was individually integrated on the long arms of Chromosomes 4A, 7A, and 5D in the three HOSUT lines. Thus, in this study we verified the chromosomal locations of the transgene, which had previously been determined by flow cytometry, and moreover revealed the chromosome-arm locations of the HOSUT transgene in the HOSUT lines.
