The Experts below are selected from a list of 4191 Experts worldwide ranked by ideXlab platform
Yiwei Qiu - One of the best experts on this subject based on the ideXlab platform.
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Effect of indomethacin and lactoferrin on human Tenocyte proliferation and collagen formation in vitro.
Biochemical and biophysical research communications, 2014Co-Authors: Yaonan Zhang, Yiwei Qiu, Xiao Wang, Andrew Carr, Jillian Cornish, Zhidao XiaAbstract:Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in patients with injuries and inflammation of tendon and ligament, and as post-surgical analgesics. The aim of this study is to investigate the effect of indomethacin, a classic NSAID and its combinational effect with an anabolic agent of skeletal tissue, lactoferrin, on the proliferation and collagen formation of human Tenocytes in vitro. A factorial experimental design was employed to study the dose-dependent effect of the combination of indomethacin and lactoferrin. The results showed that indomethacin at high concentration (100 μM) inhibited human Tenocyte proliferation in culture medium with 1-10% fetal bovine serum (FBS) in vitro. Also, high dose of indomethacin inhibited the collagen formation of human Tenocytes in 1% FBS culture medium. Lactoferrin at 50-100 μg/ml promoted human Tenocyte survival in serum-free culture medium and enhanced proliferation and collagen synthesis of human Tenocytes in 1% FBS culture medium. When 50-100 μg/ml lactoferrin was used in combination with 100-200 μM indomethacin, it partially rescued the inhibitory effects of indomethacin on human Tenocyte proliferation, viability and collagen formation. To our knowledge, it is the first evidence that lactoferrin is anabolic to human Tenocytes in vitro and reverses potential inhibitory effects of NSAIDs on human Tenocytes.
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Effects of platelet derived growth factor BB and basic fibroblast growth factor on phenotype of Tenocytes and the mRNA expression of Tenocyte phenotypic and differentiation markers
Chinese journal of experimental surgery, 2013Co-Authors: Yiwei Qiu, Liwei ZhuAbstract:Objective To evaluate the the changes of Tenocyte phenotype and the expression of the phenotypic markers by adding platelet derived growth factor BB (PDGFBB) and basic fibroblast growth factor(bFGF) to human Tenocyte in vitro culture.Methods Human Tenocytes were cultured in α-MEM medium by adding FBS at the concentration of 1％ and supplementing either/both PDGFBB and bFGF.Sirius red staining was employed to evaluate the characteristics of the Tenocytes cultured.Real-time reverse transcriptase-polymerase chain reaction (RT-qPCR) technique was used to detect the mRNA expression of the Tenocyte phenotypic and differentiation markers.Results The Tenocytes cultured for 14 days with 1％ FBS,50 μg/L PDGFBB + 50 μg/L bFGF showed less prominent collagen synthesis in comparison with those cultured in 10％ FBS.The Tenocytes cultured in the treated group also showed down-regulated mRNA expression of those markers [Scleraxis (Scx) 0.33,t =374.055 ; Tenomodulin (Tnmd) 0.003,t =28 199.418; Collagen type Ⅰ (Col Ⅰ) 0.57,t =164.195 ; Decorin (Den) 0.26,t =177.52).Conclusion These findings suggest that human Tenocytes could not only maintain their phenotype in the culture media with low concentration of FBS,but also keep the cells at less differentiated state,having this approach a suitable one for tendon tissue engineering. Key words: Tissue engineering; Tenocyte; Basic fibroblast growth factor; Differentiation
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Effects of platelet derived growth factorBB and basic fibroblast growth factor on proliferation and collagen synthesis of Tenocytes
Chinese journal of experimental surgery, 2013Co-Authors: Yiwei Qiu, Liwei ZhuAbstract:Objective To evaluate the effect of platelet derived growth factor (PDGF) BB and basic fibroblast growth factor (bFGF) on proliferation and collagen synthesis of Tenocytes in in vitro culture.Methods Human Tenocytes were cultured in α-MEM medium supplemented with fatal bovine serun (FBS) at various concentrations and both PDGFBB and bFGF.AlamarBlue was used to examine proleration of Tenocytes,and Sirius red staining was employed to evaluate the collagen synthesis of the cells studied.Results The Tenocytes cultured for 14 days with 1％ FBS + 50 μg/L PDGFBB +50 μg/L bFGF showed similar proliferation in comparison with those cultured in 10％ FBS (approximately 400％ increase).Tenocytes cultured in 50 μg/L PDGFBB + 50 μg/L bFGF showed significantly reduced collagen synthesis [(0.211 ±0.002) ng] in comparison with those cultured in 10％ FBS [(0.485 ±0.06g) ng].Conclusion These findings have demonstrated that in the presence of 50 μg/L PDGFBB + 50 μg/L bFGF in the culture medium,FBS usage can be reduced to 1％,which can not only obtain the same prolifertion rate as the 10％ FBS,but also inhibit collagen synthesis. Key words: Tenocyte; Platelet derived growth factor; Basic fibroblast growth factor; Proliferation; Collagen
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Development of a refined Tenocyte expansion culture technique for tendon tissue engineering.
Journal of tissue engineering and regenerative medicine, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:The aim of this study was to efficiently expand less differentiated Tenocytes with minimum use of fetal bovine serum (FBS) for Tenocyte-based tendon tissue engineering. To achieve this goal, human Tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGFBB, IGF-1 and bFGF. A number of growth factors were selected that could support Tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the Tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGFBB and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The Tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down-regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that Tenocytes cultured in the growth factors had reduced collagen fibril formation compared to Tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human Tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required. Copyright © 2012 John Wiley & Sons, Ltd.
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Development of a Refined Tenocyte Differentiation Culture Technique for Tendon Tissue Engineering
Cells tissues organs, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, R Rout, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:We have established that human Tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of Tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of Tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human Tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β3. These findings have shown for the first time that human Tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering.
Afsie Sabokbar - One of the best experts on this subject based on the ideXlab platform.
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Development of a refined Tenocyte expansion culture technique for tendon tissue engineering.
Journal of tissue engineering and regenerative medicine, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:The aim of this study was to efficiently expand less differentiated Tenocytes with minimum use of fetal bovine serum (FBS) for Tenocyte-based tendon tissue engineering. To achieve this goal, human Tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGFBB, IGF-1 and bFGF. A number of growth factors were selected that could support Tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the Tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGFBB and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The Tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down-regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that Tenocytes cultured in the growth factors had reduced collagen fibril formation compared to Tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human Tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required. Copyright © 2012 John Wiley & Sons, Ltd.
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Development of a Refined Tenocyte Differentiation Culture Technique for Tendon Tissue Engineering
Cells tissues organs, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, R Rout, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:We have established that human Tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of Tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of Tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human Tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β3. These findings have shown for the first time that human Tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering.
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proliferation and differentiation of human Tenocytes in response to platelet rich plasma an in vitro and in vivo study
Journal of Orthopaedic Research, 2012Co-Authors: Xiao Wang, Yiwei Qiu, Andrew Carr, Zhidao Xia, J T Triffitt, Afsie SabokbarAbstract:Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet-rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma-clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human Tenocyte in vitro. The advantage of using PRCR is that the platelet-derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human Tenocytes in vitro. The expression of specific Tenocyte markers were similar to conventional fetal bovine serum (FBS)-treated Tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR-treated Tenocytes in vivo as compared to 10% FBS-treated cells. Overall, our feasibility study has indicated that PRCR can induce human Tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries.
Ming-long Yeh - One of the best experts on this subject based on the ideXlab platform.
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The effect of Tenocyte/hyaluronic acid therapy on the early recovery of healing Achilles tendon in rats
Journal of Materials Science: Materials in Medicine, 2014Co-Authors: Jen-i Liang, Ping-chia Lin, Meng-yi Chen, Tsung-hsun Hsieh, Jia-jin Jason Chen, Ming-long YehAbstract:The aim of this study was to explore the potential for a better recovery outcome for the Achilles tendon at an early healing stage when a mixed biomaterial-Tenocyte injection is used. The experimental animals underwent single limb Achilles tendon transection followed by suturing repair. A solution of either hyaluronic acid with or without Tenocytes or normal saline was randomly chosen to be injected around the injury site after surgery. To obtain the comprehensive recovery condition of the rats on different management protocols, the animals were evaluated histologically, mechanically, and functionally. A significant difference in the recovery condition was found in the injured tendon injected with the hyaluronic acid solution with Tenocytes compared with the other groups. Tendon stiffness and the locomotion abilities of the rats with healing Achilles tendons were improved in the hyaluronic acid with Tenocyte transplantation group. The acceleration of the inflammatory phase in rats with the hyaluronic acid with Tenocyte injections might be the major reason for the better functional outcomes.
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the effect of Tenocyte hyaluronic acid therapy on the early recovery of healing achilles tendon in rats
Journal of Materials Science: Materials in Medicine, 2014Co-Authors: Jen-i Liang, Ping-chia Lin, Meng-yi Chen, Tsung-hsun Hsieh, Jia-jin Jason Chen, Ming-long YehAbstract:The aim of this study was to explore the potential for a better recovery outcome for the Achilles tendon at an early healing stage when a mixed biomaterial-Tenocyte injection is used. The experimental animals underwent single limb Achilles tendon transection followed by suturing repair. A solution of either hyaluronic acid with or without Tenocytes or normal saline was randomly chosen to be injected around the injury site after surgery. To obtain the comprehensive recovery condition of the rats on different management protocols, the animals were evaluated histologically, mechanically, and functionally. A significant difference in the recovery condition was found in the injured tendon injected with the hyaluronic acid solution with Tenocytes compared with the other groups. Tendon stiffness and the locomotion abilities of the rats with healing Achilles tendons were improved in the hyaluronic acid with Tenocyte transplantation group. The acceleration of the inflammatory phase in rats with the hyaluronic acid with Tenocyte injections might be the major reason for the better functional outcomes.
Zhidao Xia - One of the best experts on this subject based on the ideXlab platform.
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Effect of indomethacin and lactoferrin on human Tenocyte proliferation and collagen formation in vitro.
Biochemical and biophysical research communications, 2014Co-Authors: Yaonan Zhang, Yiwei Qiu, Xiao Wang, Andrew Carr, Jillian Cornish, Zhidao XiaAbstract:Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in patients with injuries and inflammation of tendon and ligament, and as post-surgical analgesics. The aim of this study is to investigate the effect of indomethacin, a classic NSAID and its combinational effect with an anabolic agent of skeletal tissue, lactoferrin, on the proliferation and collagen formation of human Tenocytes in vitro. A factorial experimental design was employed to study the dose-dependent effect of the combination of indomethacin and lactoferrin. The results showed that indomethacin at high concentration (100 μM) inhibited human Tenocyte proliferation in culture medium with 1-10% fetal bovine serum (FBS) in vitro. Also, high dose of indomethacin inhibited the collagen formation of human Tenocytes in 1% FBS culture medium. Lactoferrin at 50-100 μg/ml promoted human Tenocyte survival in serum-free culture medium and enhanced proliferation and collagen synthesis of human Tenocytes in 1% FBS culture medium. When 50-100 μg/ml lactoferrin was used in combination with 100-200 μM indomethacin, it partially rescued the inhibitory effects of indomethacin on human Tenocyte proliferation, viability and collagen formation. To our knowledge, it is the first evidence that lactoferrin is anabolic to human Tenocytes in vitro and reverses potential inhibitory effects of NSAIDs on human Tenocytes.
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Development of a refined Tenocyte expansion culture technique for tendon tissue engineering.
Journal of tissue engineering and regenerative medicine, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:The aim of this study was to efficiently expand less differentiated Tenocytes with minimum use of fetal bovine serum (FBS) for Tenocyte-based tendon tissue engineering. To achieve this goal, human Tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGFBB, IGF-1 and bFGF. A number of growth factors were selected that could support Tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the Tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGFBB and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The Tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down-regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that Tenocytes cultured in the growth factors had reduced collagen fibril formation compared to Tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human Tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required. Copyright © 2012 John Wiley & Sons, Ltd.
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Development of a Refined Tenocyte Differentiation Culture Technique for Tendon Tissue Engineering
Cells tissues organs, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, R Rout, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:We have established that human Tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of Tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of Tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human Tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β3. These findings have shown for the first time that human Tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering.
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proliferation and differentiation of human Tenocytes in response to platelet rich plasma an in vitro and in vivo study
Journal of Orthopaedic Research, 2012Co-Authors: Xiao Wang, Yiwei Qiu, Andrew Carr, Zhidao Xia, J T Triffitt, Afsie SabokbarAbstract:Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet-rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma-clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human Tenocyte in vitro. The advantage of using PRCR is that the platelet-derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human Tenocytes in vitro. The expression of specific Tenocyte markers were similar to conventional fetal bovine serum (FBS)-treated Tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR-treated Tenocytes in vivo as compared to 10% FBS-treated cells. Overall, our feasibility study has indicated that PRCR can induce human Tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries.
Andrew Carr - One of the best experts on this subject based on the ideXlab platform.
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Effect of indomethacin and lactoferrin on human Tenocyte proliferation and collagen formation in vitro.
Biochemical and biophysical research communications, 2014Co-Authors: Yaonan Zhang, Yiwei Qiu, Xiao Wang, Andrew Carr, Jillian Cornish, Zhidao XiaAbstract:Non-steroidal anti-inflammatory drugs (NSAIDs) are widely used in patients with injuries and inflammation of tendon and ligament, and as post-surgical analgesics. The aim of this study is to investigate the effect of indomethacin, a classic NSAID and its combinational effect with an anabolic agent of skeletal tissue, lactoferrin, on the proliferation and collagen formation of human Tenocytes in vitro. A factorial experimental design was employed to study the dose-dependent effect of the combination of indomethacin and lactoferrin. The results showed that indomethacin at high concentration (100 μM) inhibited human Tenocyte proliferation in culture medium with 1-10% fetal bovine serum (FBS) in vitro. Also, high dose of indomethacin inhibited the collagen formation of human Tenocytes in 1% FBS culture medium. Lactoferrin at 50-100 μg/ml promoted human Tenocyte survival in serum-free culture medium and enhanced proliferation and collagen synthesis of human Tenocytes in 1% FBS culture medium. When 50-100 μg/ml lactoferrin was used in combination with 100-200 μM indomethacin, it partially rescued the inhibitory effects of indomethacin on human Tenocyte proliferation, viability and collagen formation. To our knowledge, it is the first evidence that lactoferrin is anabolic to human Tenocytes in vitro and reverses potential inhibitory effects of NSAIDs on human Tenocytes.
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Regulation of hypoxia-induced cell death in human Tenocytes.
Advances in orthopedics, 2012Co-Authors: Min Liang, Andrew Carr, H R Cornell, N. Zargar Baboldashti, Mark S. Thompson, Philippa A. HulleyAbstract:Degenerate shoulder tendons display evidence of hypoxia. However tendons are relatively avascular and not considered to have high oxygen requirements and the vulnerability of tendon cells to hypoxia is unclear. Cultured human Tenocytes were exposed to hypoxia and the cellular response detected using QPCR, Western blotting, viability, and ELISA assays. We find that Tenocytes respond to hypoxia in vitro by activating classical HIF-1α-driven pathways. Total hypoxia caused significant Tenocyte apoptosis. Transcription factors typically involved in hypoxic response, HIF-1α and FOXO3A, were upregulated. Hypoxia caused sustained upregulation of several proapoptotic proteins known to mediate hypoxia-induced apoptosis, such as Bnip3 and Nix, but others were unchanged although they were reportedly hypoxia-sensitive in other cell types. Antiapoptotic proteins Bcl2 and Bcl-xL were unchanged by hypoxia. Normal human Tenocytes expressed all isoforms of the hypoxia-induced vascular growth factor VEGF except VEGF-D. Hypoxia markedly upregulated VEGF-A mRNA, followed by increased VEGF protein secretion. However treatment with VEGF did not improve Tenocyte survival. As a protective strategy for Tenocytes at risk of hypoxic death we added prosurvival growth factors insulin or platelet rich plasma (PRP). Both agents strongly protected Tenocytes from hypoxia-induced death over 48 h, suggesting possible efficacy in the acute postrupture tendon or integrating graft.
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Development of a refined Tenocyte expansion culture technique for tendon tissue engineering.
Journal of tissue engineering and regenerative medicine, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:The aim of this study was to efficiently expand less differentiated Tenocytes with minimum use of fetal bovine serum (FBS) for Tenocyte-based tendon tissue engineering. To achieve this goal, human Tenocytes were cultured in different concentrations of FBS and combinations of growth factors PDGFBB, IGF-1 and bFGF. A number of growth factors were selected that could support Tenocyte expansion at reduced differentiated state with minimum FBS usage. Results showed that the expansion of the Tenocytes cultured for 14 days with 1% FBS, 50 ng/ml PDGFBB and 50 ng/ml bFGF was similar to that cultured in the 10% FBS control group. The Tenocytes cultured in the treatment group showed significantly lower collagen synthesis and down-regulation of mRNA expression of tendon differentiation markers. Cell morphology confirmed that Tenocytes cultured in the growth factors had reduced collagen fibril formation compared to Tenocytes cultured in 10% FBS. Our findings confirm the feasibility of inducing human Tenocyte expansion in vitro with the least amount of FBS usage, while controlling their differentiation until required. Copyright © 2012 John Wiley & Sons, Ltd.
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Development of a Refined Tenocyte Differentiation Culture Technique for Tendon Tissue Engineering
Cells tissues organs, 2012Co-Authors: Yiwei Qiu, Xiao Wang, Yaonan Zhang, R Rout, Andrew Carr, Liwei Zhu, Zhidao Xia, Afsie SabokbarAbstract:We have established that human Tenocytes can differentiate in the absence of exogenous fetal bovine serum (FBS) but in the presence of insulin-like growth factor-1 (IGF-1) and transforming growth factor-β3 (TGF-β3). The extent of Tenocyte differentiation was assessed by examining cell survival, collagen synthesis, cell morphology and expression of Tenocyte differentiation markers such as scleraxis (Scx), tenomodulin (Tnmd), collagen type I (Col-I) and decorin (Dcn). Our results indicate that 50 ng/ml IGF-1 and 10 ng/ml TGF-β3 (in the absence of FBS) were capable of maintaining in vitro human Tenocyte survival in 14-day cultures. The extent of collagen synthesis and messenger ribonucleic acid expression of Scx, Tnmd, Col-I and Dcn were significantly upregulated in response to IGF-1 and TGF-β3. These findings have shown for the first time that human Tenocytes can be maintained in long-term culture, in serum-free conditions, making this approach a suitable one for the purpose of tendon tissue engineering.
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proliferation and differentiation of human Tenocytes in response to platelet rich plasma an in vitro and in vivo study
Journal of Orthopaedic Research, 2012Co-Authors: Xiao Wang, Yiwei Qiu, Andrew Carr, Zhidao Xia, J T Triffitt, Afsie SabokbarAbstract:Platelet rich plasma (PRP) is the autologous plasma fraction with a platelet-rich cellular component which is enriched with a number of growth factors. Due to its availability and low cost, PRP has become an increasingly popular clinical tool as an alternative source of growth factors for various applications, for example, tendon regeneration but with limited success in clinical trials. The main objective of the current study was to determine whether activated PRP [i.e., platelet rich plasma-clot release (PRCR)] could be used to induce the proliferation and collagen synthesis in human Tenocyte in vitro. The advantage of using PRCR is that the platelet-derived bioactive factors are more concentrated and could initiate a more rapid and accelerated healing response than PRP. Our results demonstrated that 10% PRCR treatment accelerated the extent of cell proliferation and collagen production by human Tenocytes in vitro. The expression of specific Tenocyte markers were similar to conventional fetal bovine serum (FBS)-treated Tenocytes implanted in mice within 14 days of implantation in diffusion chambers. Moreover, relatively more collagen fibrils were evident in PRCR-treated Tenocytes in vivo as compared to 10% FBS-treated cells. Overall, our feasibility study has indicated that PRCR can induce human Tenocyte proliferation and collagen synthesis which could be implemented for future tendon regeneration in reconstructive surgeries.
