The Experts below are selected from a list of 96 Experts worldwide ranked by ideXlab platform
Huijing Bao - One of the best experts on this subject based on the ideXlab platform.
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linc00028 regulates the development of tgfβ1 treated human Tenon Capsule fibroblasts by targeting mir 204 5p
Biochemical and Biophysical Research Communications, 2020Co-Authors: Huali Sui, Wenjing Liu, Shanshan Fan, Xuan Zhang, Huijing BaoAbstract:Glaucoma is a leading cause of blindness worldwide with complex pathogenesis. The excessive proliferation and fibrosis of human Tenon Capsule fibroblasts (HTFs) trigger the scar formation after glaucoma filtration surgery. The purpose was to investigate the role of long intergenic non-protein coding RNA 28 (LINC00028) and mechanism in transforming growth factor β1 (TGFβ1)-treated HTFs. The detection of LINC00028 and miR-204-5p expression was conducted using quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK-8) assay. Cell migration and invasion were monitored by transwell assay. The expression of Epithelial-mesenchymal transition (EMT)-related markers, including E-cadherin, α-Smooth muscle actin (α-SMA), fibronectin and β-catenin, and autophagy-related markers, including Beclin 1 and light chain 3 (LC3-II and LC3-I) at the protein level was quantified using western blot. The prediction of the relationship between LINC00028 and miR-204-5p was performed by the online tool miRcode, and the verification of the relationship between them was conducted using dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay and RNA pull-down assay. The expression of LINC00028 was elevated in glaucoma tissues and TGFβ1-treated HTFs. LINC00028 downregulation blocked proliferation, migration, invasion, EMT, fibrosis and autophagy of TGFβ1-treated HTFs. MiR-204-5p was a target of LINC00028, and its reintroduction exerted a similar role of LINC00028 downregulation. The inhibition of miR-204-5p reversed the effects of LINC00028 downregulation in TGFβ1-treated HTFs. LINC00028 regulated proliferation, migration, invasion, EMT, fibrosis and autophagy to induce the development of HTFs by competitively targeting miR-204-5p, and LINC00028 was regarded as a promising biomarker for glaucoma filtration treatment.
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tgf β2 induces proliferation and inhibits apoptosis of human Tenon Capsule fibroblast by mir 26 and its targeting of ctgf
Biomedicine & Pharmacotherapy, 2018Co-Authors: Huijing Bao, Kai Jiang, Kai Meng, Wenjing Liu, Ping Liu, Dabo WangAbstract:Abstract Aim The aim of this study was to research the effect of TGF-β2 on human enon Capsule fibroblasts proliferation and apoptosis and its potential mechanism. Methods Human eyeball fascia tissues (n = 45) were derived from ocular fascia tissues of patients who were underwent glaucoma filtration surgery, and Tenon Capsule fibroblasts were obtained from these tissues. Liposome-mediated transfection, CCK8 assay, Hoechst33258 staining, qRT-PCR detection, western blot, and luciferase reporter assay were performed. Results TGF-β2 promoted proliferation and inhibited apoptosis of human Tenon Capsule fibroblasts in a dose-dependent manner. TGF-β2 induced down-regulation of miR-26 and up-regulation of CTGF in a dose-dependent manner. CTGF was the target gene of miR-26 and miR-26 had a negative regulatory effect on CTGF expression. miR-26 up-regulation could significantly decrease proliferation and increase apoptosis of human Tenon Capsule fibroblasts after induced by TGF-β2 (P Conclusion miR-26 could inhibit proliferation and promote apoptosis of human Tenon Capsule fibroblasts after they were induced by TGF-β2 through suppressing CTGF expression.
Huaijin Guan - One of the best experts on this subject based on the ideXlab platform.
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rna interference targeting connective tissue growth factor inhibits the transforming growth factor β2 induced proliferation in human Tenon Capsule fibroblasts
Journal of Ophthalmology, 2013Co-Authors: Jiaona Jing, Yuncheng Sun, Huaijin GuanAbstract:Purpose. This study was to determine the effect of CTGF-small interfering RNA (siRNA) on TGF-β2-induced proliferation in human Tenon Capsule fibroblasts (HTFs). Methods. HTFs were transfected with four of CTGF-siRNAs separately for screening of gene silencing efficacy that was determined by transcript level measured by quantitative real-time PCR (qRT-PCR). Recombinant TGF-β2 was added into the culture to stimulate the proliferation of HTFs. The gene silencing efficacy of the siRNAs was evaluated by qRT-PCR and immunofluorescence of CTGF transcript and protein levels. The viability of HTFs was determined by cell counting kit-8 (CCK-8). FCM was used to assess cell cycle after CTGF-siRNA transfection. Results. The expression of CTGF and proliferation of HTFs were increased significantly by TGF-β2 stimulation. The transfection of CTGF-siRNA abolished the upregulation of CTGF and cell proliferation induced by TGF-β2. The analysis of cell cycle indicated that CTGF-siRNA treatment stimulated cells from S phase to G0/G1 phase in comparison with the inverse physiologic function of TGF-β2. Conclusion. CTGF targeting siRNA could effectively suppress the expression of CTGF and attenuate the proliferation of HTFs. The siRNA approach may provide a therapeutic option for eliminating filtration bleb scarring after glaucoma filtration surgery (GFS).
Jonathan G Crowston - One of the best experts on this subject based on the ideXlab platform.
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apoptosis gene expression and death receptor signaling in mitomycin c treated human Tenon Capsule fibroblasts
Investigative Ophthalmology & Visual Science, 2002Co-Authors: Jonathan G Crowston, Lydia H Chang, P H Constable, Julie T Daniels, Arne N Akbar, Peng T KhawAbstract:PURPOSE. To examine the effect of mitomycin-C on the expression of apoptosis genes in human Tenon Capsule fibroblasts and to evaluate whether death receptor signaling modulates mitomycin-C cytotoxicity.METHODS. Bcl-2, Bax, Bcl-x, Fas (CD95) and tumor necrosis factor (TNF) receptor expression was determined by flow cytometry in control and mitomycin-C-treated Tenon fibroblasts. Fibroblast death was quantified using a lactate dehydrogenase release assay. The effect of Fas and TNF-receptor signaling was evaluated using Fas-specific antibodies and soluble TNF-alpha.RESULTS. Tenon fibroblasts constitutively express Bcl-2, Bax, and Bcl-x in culture. Mitomycin-C (0.4 mg/mL) induced a small but consistent increase in the expression of all three proteins. Tenon fibroblasts express low levels of Fas but are resistant to the effects of Fas-receptor ligation. Mitomycin-C (0.01-1.0 mg/ mL led to a significant increase in Fas expression at all concentrations tested (P < 0.01). Pretreatment with mitomycin-C (0.4 mg/mQ rendered fibroblasts susceptible to agonistic anti-Fas monoclonal IgM antibodies (50-500 ng/mL and led to a further 50% reduction in viable fibroblasts at 48 hours, compared with mitomycin-C alone (P 0.<05). Antibodies that block the Fas receptor did not inhibit mitomycin-C-induced apoptosis.CONCLUSIONS. Mitomycin-C alters apoptosis gene expression and primes fibroblasts to the effects of Fas receptor ligation. Factors other than the level of Fas receptor expression modulate the response to Fas receptor signaling. Determining the signals that regulate fibroblast apoptosis may help to reline therapeutic strategies for switching off the subconjunctival healing response and maintaining intraocular pressure control.
Hanem Kishk - One of the best experts on this subject based on the ideXlab platform.
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supra Tenon Capsule implantation of the ahmed glaucoma valve in refractory pediatric glaucoma
Journal of Glaucoma, 2016Co-Authors: Eman M Elhefney, Hossam T Alsharkawy, Hanem KishkAbstract:Purpose To evaluate the efficacy of supra-Tenon Capsule implantation of an Ahmed glaucoma valve (AGV) as a measure to decrease the fibrotic potential of the Tenon Capsule on bleb formation and its subsequent effect on intraocular pressure (IOP) control in children with refractory glaucoma. Setting Mansoura Ophthalmic Centre, Faculty of Medicine, Mansoura University, Egypt. Design A prospective interventional study. Patients and methods Twenty-two eyes of 12 children with refractory glaucoma underwent supra-Tenon Capsule implantation of AGV. Ophthalmic examinations under general anesthesia including measurement of the corneal diameter and the IOP with Perkin's tonometer were performed preoperatively, on the first postoperative day, the first postoperative week, weekly for the first month, 2-weekly for the following 3 months, and monthly for at least 18 months. Postoperative complications and the number of glaucoma medications used preoperatively and postoperatively were recorded. The paired Student t test was used to compare preoperative and postoperative data. Results There were 12 eyes (54.6%) with refractory congenital glaucoma, 7 eyes (31.8%) with refractory pseudophakic glaucoma, and 3 eyes (13.6%) with refractory aphakic glaucoma. Patients included 10 male (83.3%) and 2 female (16.7%) children with a mean age of 16.3±9.7 months. The mean follow-up duration was 24.1±4.3 months. There was a statistically significant difference between the mean preoperative IOP (30.7±2.88 mm Hg) and the mean postoperative IOP (16.1±3.60 mm Hg) (t=16.22 and P=0.000, with a mean decrease in the IOP by 47.6%). The difference between the mean number of antiglaucoma medications before surgery (1.86±0.4) and after surgery (1.0±0.9) was also statistically significant (t=4.31 and P=0.000). Total success was achieved in 18 eyes (81.9%). Postoperative complications included tube exposure and slippage (10%), hypotony (10%), and hyphema (5%). Conclusions Supra-Tenon Capsule implantation of the AGV was successful in controlling the IOP with few postoperative complications in the management of children with refractory glaucoma.
Dabo Wang - One of the best experts on this subject based on the ideXlab platform.
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tgf β2 induces proliferation and inhibits apoptosis of human Tenon Capsule fibroblast by mir 26 and its targeting of ctgf
Biomedicine & Pharmacotherapy, 2018Co-Authors: Huijing Bao, Kai Jiang, Kai Meng, Wenjing Liu, Ping Liu, Dabo WangAbstract:Abstract Aim The aim of this study was to research the effect of TGF-β2 on human enon Capsule fibroblasts proliferation and apoptosis and its potential mechanism. Methods Human eyeball fascia tissues (n = 45) were derived from ocular fascia tissues of patients who were underwent glaucoma filtration surgery, and Tenon Capsule fibroblasts were obtained from these tissues. Liposome-mediated transfection, CCK8 assay, Hoechst33258 staining, qRT-PCR detection, western blot, and luciferase reporter assay were performed. Results TGF-β2 promoted proliferation and inhibited apoptosis of human Tenon Capsule fibroblasts in a dose-dependent manner. TGF-β2 induced down-regulation of miR-26 and up-regulation of CTGF in a dose-dependent manner. CTGF was the target gene of miR-26 and miR-26 had a negative regulatory effect on CTGF expression. miR-26 up-regulation could significantly decrease proliferation and increase apoptosis of human Tenon Capsule fibroblasts after induced by TGF-β2 (P Conclusion miR-26 could inhibit proliferation and promote apoptosis of human Tenon Capsule fibroblasts after they were induced by TGF-β2 through suppressing CTGF expression.
