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David Bunick - One of the best experts on this subject based on the ideXlab platform.
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Triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal Sertoli cells: possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment.
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, the directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro
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triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal sertoli cells possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:: Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, we directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro. Sertoli cells from 5-day-old rat testes were grown in serum-free medium alone (controls) or with additional triiodothyronine (T3; 1-200 nM) and/or FSH (1 microgram/ml). After 4 days, cultures were used to obtain RNA for Northern hybridization or for thymidine autoradiography. Labeling index (LI) for control cultures and cultures receiving 100 nM T3 alone was 5.2 +/- 0.5% and 5.0 +/- 0.4%, respectively. The LI of FSH-treated cultures increased to 8.4 +/- 0.8% (p < 0.01 vs. control). Cultures treated with FSH and 1, 10, 100, or 200 nM T3 had LIs of 8.0 +/- 0.9%, 6.1 +/- 0.4%, 5.3 +/- 0.6%, and 4.8 +/- 0.6%, respectively; the last three values were less than for cells receiving FSH alone (p < 0.01) or FSH + 1 nM T3 (p < 0.05). Northern hybridization indicated that mRNA levels for clusterin and inhibin-beta B, Sertoli cell secretory proteins whose production normally increases during postnatal differentiation in vivo, were significantly increased by T3 or FSH alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Jun-ichi Suto - One of the best experts on this subject based on the ideXlab platform.
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Effects of quantitative trait loci determining testicular Weight in DDD/Sgn inbred mice are strongly influenced by circulating testosterone levels
Asian-australasian Journal of Animal Sciences, 2019Co-Authors: Jun-ichi Suto, Misaki KojimaAbstract:Testicular growth and development are strongly influenced by androgen. Although both Testis Weight and plasma testosterone level are inherited traits, the interrelationship between them is not fully established. Males of DDD/Sgn (DDD) mice are known to have extremely heavy testes and very high plasma testosterone level among inbred mouse strains. We dissected the genetic basis of Testis Weight and analyzed the potential influence of plasma testosterone level in DDD mice. Quantitative trait loci (QTL) mapping of Testis Weight was performed with or without considering the influence of plasma testosterone level in reciprocal F2 intercross populations between DDD and C57BL/6J (B6) mice, thereby assessing the influence of testosterone on the effect of Testis Weight QTL. Candidate genes for Testis Weight QTL were investigated by next-generation sequencing analysis. Four significant QTL were identified on chromosomes 1, 8, 14, and 17. The DDD-derived allele was associated with increased Testis Weight. The F2 mice were then divided into two groups according to the plasma testosterone level (F2 mice with relatively "low" and "high" testosterone level), and QTL scans were again performed. Although QTL on chromosomes 1 was shared in both F2 mice, QTL on chromosomes 8 and 17 were identified specifically in F2 mice with relatively high testosterone levels. By whole-exome sequencing analysis, we identified one DDD-specific missense mutation Pro29Ser in Atat1. Most of the Testis Weight QTL expressed stronger phenotypic effect when they were placed on circumstance with high testosterone level. High testosterone influenced the QTL by enhancing the effect of DDD-derived allele and diminishing the effects of B6-derived allele. Since Pro29Ser was not identified in other inbred mouse strains, and since Pro29 in Atat1 has been strongly conserved among mammalian species, Atat1 is a plausible candidate for Testis Weight QTL on chromosome 17.
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effect of the y chromosome on Testis Weight in mice
Journal of Veterinary Medical Science, 2015Co-Authors: Kunio Satou, Jun-ichi SutoAbstract:: We investigated the effect of the Y chromosome on Testis Weight in (B6.Cg-A(y) × Y-consomic mouse strain) F1 male mice. We obtained the following results: (1) Mice with the Mus musculus domesticus-type Y chromosome had significantly heavier Testis than those with the M. m. musculus-type Y chromosome. (2) Variations in Usp9y and the number of CAG repeats in Sry were significantly associated with testes Weight. The A(y) allele was correlated with a reduced Testis Weight, and the extent of this reduction was significantly associated with a CAG repeat number polymorphism in Sry. These results suggest that Y chromosome genes not only influence Testis Weight but also modify the effect of the A(y) allele in mediating this phenomenon.
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Genetic dissection of Testis Weight in mice: quantitative trait locus analysis using F_2 intercrosses between strains with extreme Testis Weight, and association study using Y-consomic strains
Mammalian Genome, 2011Co-Authors: Jun-ichi SutoAbstract:In the present study, dissection of genetic bases of Testis Weight in mice was performed. Autosomes and the X chromosome were searched using traditional quantitative trait locus (QTL) scans, and the Y chromosome was searched by association studies of Y-consomic strains. QTL analysis was performed in ♀DDD × ♂CBA F_2 mice; the inbred mouse DDD has the heaviest testes, whereas the inbred mouse CBA has the lightest testes. Two significant Testis Weight QTLs were identified on chromosomes 1 and X. A DDD allele was associated with increased and decreased Testis Weight at the locus on chromosomes 1 and X, respectively. In the reciprocal cross ♀CBA × ♂DDD F_2 mice, QTL on chromosome 1, and not on chromosome X, had a significant effect on Testis Weight. The DDD allele at the X-linked locus could not sustain Testis Weight in combination with the Y chromosome of the CBA strain. The Y chromosome per se had a significant effect on Testis Weight, i.e., DH-Chr Y^DDD had significantly heavier testes than DH-Chr Y^CBA. On the basis of the results of Y-chromosome-wide association studies using 17 Y-consomic strains, variations in Uty , Usp9y , and Sry were significantly associated with Testis Weight. Thus, Testis Weight is a complex quantitative phenotype controlled by multiple genes on autosomes and sex chromosomes and their interactions.
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genetic dissection of Testis Weight in a mouse strain having an extremely large Testis major Testis Weight determinants are autosomal rather than y linked on the basis of comprehensive analyses in y chromosome consomic strains
Proceedings of the Japan Academy. Series B Physical and biological sciences, 2008Co-Authors: Jun-ichi SutoAbstract:I investigated the potential contribution of Y-linked genes by analyzing 16 Y-consomic strains that had been established on a DH-strain background. The results provided evidence that only the Y chromosome from the C3H/HeJ strain was different from most other inbred strains. The CBA strain has the lightest Testis and the DDD strain has the heaviest Testis among mouse strains; however, Y-consomic analysis revealed that there were no significant differences in Testis Weight among DH, DH-Chr YDDD, and DH-Chr YCBA strains, suggesting that YDDD and YCBA themselves do not influence Testis Weight. QTL analysis in DDD × DH F2 mice identified significant Testis Weight QTLs on chromosomes 9, 14, and 17, and the DDD allele at all these loci was associated with an increase in Testis Weight. Contribution of Y chromosome itself to Testis Weight was thus rather modest, and therefore, major Testis Weight determinants are autosomal. However, it was uncertain whether there would be any effects by interactions between Y chromosomal and autosomal genes.
Paul S. Cooke - One of the best experts on this subject based on the ideXlab platform.
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Neonatal Polychlorinated Biphenyl Treatment Increases Adult Testis Size and Sperm Production in the Rat
Toxicology and Applied Pharmacology, 1996Co-Authors: Paul S. Cooke, Yi-dong Zhao, Larry G. HansenAbstract:Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants which decrease serum thyroxine (T4) concentrations. We have previously demonstrated that neonatal hypothyroidism in the rat increases Sertoli cell numbers, adult Testis Weight, and daily sperm production (DSP). The aim of this study was to determine if neonatal PCB treatment increases adult Testis Weight and DSP. Treated rats received either Aroclor 1242 or 1254 (0.4–3.2 mg/day), from birth to Day 25 by daily injection; some treated litters also received T4replacement. Controls received vehicle alone. Tritiated thymidine autoradiography was used to assess Sertoli cell proliferation in 15-day control and Aroclor-treated rats. Serum T4was measured at 25, 45, and 135 days of age, and serum testosterone, Testis Weight, DSP, and testicular histology were examined at 135 days. Both Aroclor 1242 and 1254 suppressed serum T4concentrations; Aroclor 1254 was more potent and long lasting. Testis Weight was increased 22 and 13% in rats that received the 1.6 and 3.2 mg/day Aroclor 1242 doses, respectively, while the 0.4 mg/day dose did not produce significant increases. Aroclor 1254 produced significant increases in Testis Weight of 13 and 23% at the 0.4 and 1.6 mg/day doses, respectively. The 1.6 mg/day Aroclor 1242 and the 0.4 and 1.6 mg/day Aroclor 1254 doses increased DSP by 27, 11, and 42%, respectively; other treatments did not produce significant increases. At 15 days of age, Sertoli cell proliferation was greater in treated rats than in controls. T4replacement decreased or eliminated the increased Testis Weight and DSP seen in Aroclor-treated rats. The highest dose of Aroclor 1242 and both doses of Aroclor 1254 decreased adult body Weight, while other treatments did not. These results indicate that neonatal PCB treatment increases adult Testis Weight and DSP in rats. PCBs produce this effect primarily by inducing hypothyroidism, which leads to increased Sertoli cell proliferation, Testis Weight, and DSP. Thus PCBs, despite inhibitory effects on adult reproductive organs, can paradoxically stimulate increases in adult Testis Weight and DSP when administered neonatally. These data emphasize the pleiotropic nature of PCB effects and the susceptibility of the developing reproductive system to environmental factors.
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Triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal Sertoli cells: possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment.
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, the directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro
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triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal sertoli cells possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:: Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, we directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro. Sertoli cells from 5-day-old rat testes were grown in serum-free medium alone (controls) or with additional triiodothyronine (T3; 1-200 nM) and/or FSH (1 microgram/ml). After 4 days, cultures were used to obtain RNA for Northern hybridization or for thymidine autoradiography. Labeling index (LI) for control cultures and cultures receiving 100 nM T3 alone was 5.2 +/- 0.5% and 5.0 +/- 0.4%, respectively. The LI of FSH-treated cultures increased to 8.4 +/- 0.8% (p < 0.01 vs. control). Cultures treated with FSH and 1, 10, 100, or 200 nM T3 had LIs of 8.0 +/- 0.9%, 6.1 +/- 0.4%, 5.3 +/- 0.6%, and 4.8 +/- 0.6%, respectively; the last three values were less than for cells receiving FSH alone (p < 0.01) or FSH + 1 nM T3 (p < 0.05). Northern hybridization indicated that mRNA levels for clusterin and inhibin-beta B, Sertoli cell secretory proteins whose production normally increases during postnatal differentiation in vivo, were significantly increased by T3 or FSH alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Yi-dong Zhao - One of the best experts on this subject based on the ideXlab platform.
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Neonatal Polychlorinated Biphenyl Treatment Increases Adult Testis Size and Sperm Production in the Rat
Toxicology and Applied Pharmacology, 1996Co-Authors: Paul S. Cooke, Yi-dong Zhao, Larry G. HansenAbstract:Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants which decrease serum thyroxine (T4) concentrations. We have previously demonstrated that neonatal hypothyroidism in the rat increases Sertoli cell numbers, adult Testis Weight, and daily sperm production (DSP). The aim of this study was to determine if neonatal PCB treatment increases adult Testis Weight and DSP. Treated rats received either Aroclor 1242 or 1254 (0.4–3.2 mg/day), from birth to Day 25 by daily injection; some treated litters also received T4replacement. Controls received vehicle alone. Tritiated thymidine autoradiography was used to assess Sertoli cell proliferation in 15-day control and Aroclor-treated rats. Serum T4was measured at 25, 45, and 135 days of age, and serum testosterone, Testis Weight, DSP, and testicular histology were examined at 135 days. Both Aroclor 1242 and 1254 suppressed serum T4concentrations; Aroclor 1254 was more potent and long lasting. Testis Weight was increased 22 and 13% in rats that received the 1.6 and 3.2 mg/day Aroclor 1242 doses, respectively, while the 0.4 mg/day dose did not produce significant increases. Aroclor 1254 produced significant increases in Testis Weight of 13 and 23% at the 0.4 and 1.6 mg/day doses, respectively. The 1.6 mg/day Aroclor 1242 and the 0.4 and 1.6 mg/day Aroclor 1254 doses increased DSP by 27, 11, and 42%, respectively; other treatments did not produce significant increases. At 15 days of age, Sertoli cell proliferation was greater in treated rats than in controls. T4replacement decreased or eliminated the increased Testis Weight and DSP seen in Aroclor-treated rats. The highest dose of Aroclor 1242 and both doses of Aroclor 1254 decreased adult body Weight, while other treatments did not. These results indicate that neonatal PCB treatment increases adult Testis Weight and DSP in rats. PCBs produce this effect primarily by inducing hypothyroidism, which leads to increased Sertoli cell proliferation, Testis Weight, and DSP. Thus PCBs, despite inhibitory effects on adult reproductive organs, can paradoxically stimulate increases in adult Testis Weight and DSP when administered neonatally. These data emphasize the pleiotropic nature of PCB effects and the susceptibility of the developing reproductive system to environmental factors.
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Triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal Sertoli cells: possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment.
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, the directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro
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triiodothyronine inhibits proliferation and stimulates differentiation of cultured neonatal sertoli cells possible mechanism for increased adult Testis Weight and sperm production induced by neonatal goitrogen treatment
Biology of Reproduction, 1994Co-Authors: Paul S. Cooke, Yi-dong Zhao, David BunickAbstract:: Transient neonatal hypothyroidism in the rat causes prolonged Sertoli cell proliferation, delayed Sertoli cell maturation, and increased adult Sertoli cell number, Testis Weight, and sperm production. Conversely, neonatal hyperthyroidism decreases Sertoli cell proliferation and ultimate Testis size. This suggests that thyroid hormones might normally directly inhibit Sertoli cell proliferation while promoting maturation. However, these Sertoli cell effects could be due to secondary hormonal or metabolic effects of hypo- or hyperthyroidism. In this study, we directly tested thyroid hormone effects on Sertoli cell proliferation and differentiation in vitro. Sertoli cells from 5-day-old rat testes were grown in serum-free medium alone (controls) or with additional triiodothyronine (T3; 1-200 nM) and/or FSH (1 microgram/ml). After 4 days, cultures were used to obtain RNA for Northern hybridization or for thymidine autoradiography. Labeling index (LI) for control cultures and cultures receiving 100 nM T3 alone was 5.2 +/- 0.5% and 5.0 +/- 0.4%, respectively. The LI of FSH-treated cultures increased to 8.4 +/- 0.8% (p < 0.01 vs. control). Cultures treated with FSH and 1, 10, 100, or 200 nM T3 had LIs of 8.0 +/- 0.9%, 6.1 +/- 0.4%, 5.3 +/- 0.6%, and 4.8 +/- 0.6%, respectively; the last three values were less than for cells receiving FSH alone (p < 0.01) or FSH + 1 nM T3 (p < 0.05). Northern hybridization indicated that mRNA levels for clusterin and inhibin-beta B, Sertoli cell secretory proteins whose production normally increases during postnatal differentiation in vivo, were significantly increased by T3 or FSH alone.(ABSTRACT TRUNCATED AT 250 WORDS)
Ghaffari Novin Marefat - One of the best experts on this subject based on the ideXlab platform.
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the effect of busulfan on body Weight Testis Weight and mda enzymes in male rats
International Journal of Women's Health, 2014Co-Authors: Hosseini Ahar Nasibeh, Khaki Arash, Akbari Ghasem, Ghaffari Novin MarefatAbstract:Objectives: Busulfan is one of the anti-cancer drugs, despite of the tremendous usefulness in biotechnology and therapy of chronic diseases such as leukemia, lymphoma, ovarian cancer and it can lead to impaired spermatogenesis. The aim of this study was to seen body and testicular Weight and serum Malondialdehyde (MDA) enzyme in rat was performed following the use of busulfan. Material and Methods: This study was conducted on 20 adult male rats ranging in age from 6 to 8 weeks , Animals in the two group includes a control group and the experimental group that received 50 mg/ kg busulfan as a single intra peritoneal injection and after 8 weeks, body and Testis Weight and serum MDA levels were measured. Then Data were analyzed with SPSS 16. P 0.05). Testicular Weight in both control and experimental groups, respectively, were 1.45 gr and gr 0 .960. Difference between the two groups was significant decreased (P>0.05). Comparison of serum MDA of control and experimental groups, were showed 3.81±1.5 and 6.9±1.1 nmol/lit. No difference between the two groups was significant increased (P>0.05). Conclusion: It can be concluded that the use of busulfan can reduced body Weight and testicular Weight, and increased serum MDA and could be side effects in reproduction process.