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Adi Winarto - One of the best experts on this subject based on the ideXlab platform.

  • Aktivitas NADH- Tetrazolium Reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching
    2012
    Co-Authors: Roza Helmita, Ita Djuwita, Bambang Purwantara, Adi Winarto
    Abstract:

    Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated that between 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the activity of themitochondrial NADH-TetrazoliumReductase, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at 37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysis of NADH-Tetrazolium Reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed that the activity of NADH-Tetrazolium Reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P

  • aktivitas nadh Tetrazolium Reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching
    Jurnal Anatomi Indonesia, 2012
    Co-Authors: Roza Helmita, Ita Djuwita, Bambang Purwantara, Adi Winarto
    Abstract:

    Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated that between 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the activity of themitochondrial NADH-TetrazoliumReductase, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at 37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysis of NADH-Tetrazolium Reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed that the activity of NADH-Tetrazolium Reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05). The trophoblast outgrowth diameter of 24 h hatched blastocystwas significantly higher than the non hatched blastocyst, but not signifcantly different with the 48 h hatched.Morphology examination using light microscope showed that the trophoblast monolayer of 24 h hatched blastocyst differentiated into cytotrophoblast, syncytioptrophoblast and spongiotrophoblast after 5 days of in vitro cultured. In conclusion, in in vitro sudy the failure of blastocysts implantationwas due to the impairment ofNADHdehydrogenase activity in complex Imitochondria and the failure of outgrowth and differentiation of the trofoblast cells.

Rossen Stefanov - One of the best experts on this subject based on the ideXlab platform.

Roza Helmita - One of the best experts on this subject based on the ideXlab platform.

  • Aktivitas NADH- Tetrazolium Reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching
    2012
    Co-Authors: Roza Helmita, Ita Djuwita, Bambang Purwantara, Adi Winarto
    Abstract:

    Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated that between 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the activity of themitochondrial NADH-TetrazoliumReductase, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at 37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysis of NADH-Tetrazolium Reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed that the activity of NADH-Tetrazolium Reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P

  • aktivitas nadh Tetrazolium Reductase sel sel trofoblas pada blastosis yang mengalami hatching dan gagal hatching
    Jurnal Anatomi Indonesia, 2012
    Co-Authors: Roza Helmita, Ita Djuwita, Bambang Purwantara, Adi Winarto
    Abstract:

    Implantation is themost critical stage in the establishment of pregnancy. Inmammals, it has been estimated that between 25%and 60%of conceptuses are lost before or at the time of implantation. The objectives of this study were to investigate the activity of themitochondrial NADH-TetrazoliumReductase, the outgrowth and differentiation of trophoblast cells in in vitro culture of hatched and non hatched blastocyst. Blastocysts were collected from mice cornua utery at day-4 of pregnancy and were divided into 3 groups: blastocysts undergo hatching within 24 hours, 48 hours and non hatching. Embryos were cultured in DMEMmediumin 5%CO2 incubator at 37°C for 10 days.The trophoblastsmonolayer were processed forGimsa staining and histochemistry analysis of NADH-Tetrazolium Reductase activity. The outgrowth of trophoblast cells were measured using eyespiece micrometer. The results showed that the activity of NADH-Tetrazolium Reductase of 24 h and 48 h hatched blastocysts showed higher intensity than the non hatched blastocysts (P<0,05). The trophoblast outgrowth diameter of 24 h hatched blastocystwas significantly higher than the non hatched blastocyst, but not signifcantly different with the 48 h hatched.Morphology examination using light microscope showed that the trophoblast monolayer of 24 h hatched blastocyst differentiated into cytotrophoblast, syncytioptrophoblast and spongiotrophoblast after 5 days of in vitro cultured. In conclusion, in in vitro sudy the failure of blastocysts implantationwas due to the impairment ofNADHdehydrogenase activity in complex Imitochondria and the failure of outgrowth and differentiation of the trofoblast cells.

Dubovska M - One of the best experts on this subject based on the ideXlab platform.

  • Application of NADH Tetrazolium Reductase reaction in perioperative biopsy of dysganglionic large bowel.
    European journal of pediatric surgery : official journal of Austrian Association of Pediatric Surgery ... [et al] = Zeitschrift fur Kinderchirurgie, 1994
    Co-Authors: D. Dudorkinová, Skába R, Lojda Z, Dubovska M
    Abstract:

    La reaction par la NADH-Tetrazolium Reductase a ete utilisee chez 24 patients pour preciser la presence de cellules ganglionnaires dans les plexus myenteriques au cours de 24 operations pour intestin dysganglionnaire. Les avantages de cette methode sont la preparation rapide du moyen d'incubation, le temps d'incubation reduit et la bonne demonstration de cellules ganglionnaires. Il a ete possible de determiner l'extension des parties aganglionnaires et hypoganglionnaires de l'intestin de telle sorte que les resultats s'en sont ameliores. La reaction NADH-Tetrazolium Reductase peut etre proposee pour les biopsies pre-operatoires de l'intestin dysganglionnaire

Sangkot Marzuki - One of the best experts on this subject based on the ideXlab platform.

  • Microphotometric analysis of NADH-Tetrazolium Reductase deficiency in fibroblasts of patients with Leber hereditary optic neuropathy.
    Journal of inherited metabolic disease, 2000
    Co-Authors: Safarina G Malik, Herawati Sudoyo, Sangkot Marzuki
    Abstract:

    We employed a microphotometric approach to examine whether a defect in the mitochondrial respiratory complex I expected in Leber hereditary optic neuropathy (LHON) as the consequence of a mtDNA (11778G>A) mutation in the ND4 gene coding for a subunit of the respiratory complex I can be detected at the single-cell level. Genetically stable fibroblast cell lines were established from skin biopsies of two members of a Chinese Indonesian family with LHON. The fibroblasts were homoplasmic for the 11778G>A mutation. The activity of the respiratory complex I was examined histochemically by staining for NADH-Tetrazolium Reductase. The histochemical staining showed a typical pattern with an apparent concentration of the activity around the nucleus, suggested as the reflection of the gradient in the thickness of the unsectioned fibroblast cells. Microphotometric quantification of the staining intensity showed that the activity is linear for at least 60min. The activity shows a discontinuity in its Arrhenius kinetics with a break point at 13.0–13.5°C (activation energy at 50–58 J/mol and 209–238 J/mol above and below the break temperature, respectively), indicating the membrane association of the NADH-Tetrazolium Reductase activity. Both patients showed lower fibroblast NADH-Tetrazolium Reductase activity, with a reduction of ∼30%. Our results demonstrate the utility of microphotometric analysis in the study of biochemical defects associated with mutations in the mtDNA.