The Experts below are selected from a list of 200826 Experts worldwide ranked by ideXlab platform
Ke Zhang - One of the best experts on this subject based on the ideXlab platform.
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increased th17 and Th22 Cell percentages predict acute lung injury in patients with sepsis
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P < 0.05). They were significant factors affecting sepsis-induced ALI (P < 0.05). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR = 2.612) and the percentages of Th17 (OR = 2.211) and Th22 (OR = 3.230) Cells were independent risk factors for ALI. The area under the curve of Th22 Cells was 0.844 to predict ALI with a cutoff value of 6.81%. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by the Th22 Cell percentage were 78.72% and 89.13%, respectively. CONCLUSIONS Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with ALI and they may be used as biomarkers for early diagnosis of sepsis-induced ALI.
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Increased Th17 and Th22 Cell Percentages Predict Acute Lung Injury in Patients with Sepsis.
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P
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Increased Th17 and Th22 Cell levels predict acute lung injury in patients with sepsis
2019Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:Abstract Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 Cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 Cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 Cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 Cells and Th22 Cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 Cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 Cells (P < 0.05), and the levels of Th17 Cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) Cells were independent risk factor for ALI. The area under the curve of Th22 Cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 Cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 Cells (P > 0.05). Conclusions: The levels of Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.
Liangtian Zhang - One of the best experts on this subject based on the ideXlab platform.
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increased th17 and Th22 Cell percentages predict acute lung injury in patients with sepsis
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P < 0.05). They were significant factors affecting sepsis-induced ALI (P < 0.05). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR = 2.612) and the percentages of Th17 (OR = 2.211) and Th22 (OR = 3.230) Cells were independent risk factors for ALI. The area under the curve of Th22 Cells was 0.844 to predict ALI with a cutoff value of 6.81%. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by the Th22 Cell percentage were 78.72% and 89.13%, respectively. CONCLUSIONS Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with ALI and they may be used as biomarkers for early diagnosis of sepsis-induced ALI.
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Increased Th17 and Th22 Cell Percentages Predict Acute Lung Injury in Patients with Sepsis.
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P
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Increased Th17 and Th22 Cell levels predict acute lung injury in patients with sepsis
2019Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:Abstract Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 Cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 Cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 Cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 Cells and Th22 Cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 Cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 Cells (P < 0.05), and the levels of Th17 Cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) Cells were independent risk factor for ALI. The area under the curve of Th22 Cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 Cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 Cells (P > 0.05). Conclusions: The levels of Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.
Qiaoling Zhou - One of the best experts on this subject based on the ideXlab platform.
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Acteoside relieves mesangial Cell injury by regulating Th22 Cell chemotaxis and proliferation in IgA nephropathy.
Renal failure, 2018Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Huimin Luo, Qiaoling ZhouAbstract:The existing therapies of IgA nephropathy are unsatisfying. Acteoside, the main component of Rehmannia glutinosa with anti-inflammatory and anti-immune effects, can improve urinary protein excretion and immune disorder. Th22 Cell is involved in IgA nephropathy progression. This study was determined to explore the effect of acteoside on mesangial injury underlying Th22 Cell disorder in IgA nephropathy. Serum Th22 Cells and urine total protein of patients with IgA nephropathy were measured before and after six months treatment of Rehmannia glutinosa acteoside or valsartan. Chemotactic assay and co-culture assay were performed to investigate the effect of acteoside on Th22 Cell chemotaxis and differentiation. The expression of CCL20, CCL22 and CCL27 were analyzed. To explore the effect of acteoside on mesangial Cell injury induced by inflammation, IL-1, IL-6, TNF-α and TGF-β1 were tested. Results showed that the proteinuria and Th22 lymphocytosis of patients with IgA nephropathy significantly improved after combination treatment of Rehmannia glutinosa acteoside and valsartan, compared with valsartan monotherapy. In vitro study further demonstrated that acteoside inhibit Th22 Cell chemotaxis by suppressing the production of Th22 Cell attractive chemokines, i.e., CCL20, CCL22 and CCL27. In addition, acteoside inhibited the Th22 Cell proliferation. Co-culture assay proved that acteoside could relieve the overexpression of pro-inflammatory cytokines, and prevent the synthesis of TGF-β1. TGF-β1 level in mesangial Cells was positively correlated with the Th22 Cell. This research demonstrated that acteoside can alleviate mesangial Cell inflammatory injury by modulating Th22 lymphocytes chemotaxis and proliferation.
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Tonsillitis exacerbates renal injury in IgA nephropathy through promoting Th22 Cells chemotaxis.
International urology and nephrology, 2018Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Huiming Luo, Fengmin Shao, Qiaoling ZhouAbstract:BACKGROUND Tonsillitis can promote the progression of IgA nephropathy (IgAN) by aggravating immunopathologic response. Th22 Cell disorder is involved in the pathogenesis of IgAN with tonsillitis. This study was determined to explore the possible mechanism of IgAN with tonsillitis underlying Th22 Cell chemotaxis response to the effect of CCL20, CCL22, and CCL27. METHODS This research was conducted on 65 subjects including 16 healthy controls (HC group), 5 patients with renal carcinoma (HTC group) and 44 patients with IgAN between 2015 and 2016. According to clinical symptoms and results of throat swab culture, patients with IgAN were divided into two groups: IgAN with tonsillitis (IgAN + tonsillitis, n = 14) and IgAN patients without tonsillitis (IgAN, n = 30). Distribution of Th22 Cells in IgAN patients was determined. The expression of CCL20, CCL22, and CCL27 in both peripheral blood and kidneys of IgAN patients was investigated. Severity of pathological lesions in IgAN patients was analyzed. Coculture assay and transwell assay were performed to explore the impacts of human mesangial Cells (HMC) on Th22 Cell chemotaxis and Th22 Cell local accumulation under hemolytic streptococcus (HS) infection. RESULTS Th22 Cell percentages in IgAN patients increased compared with healthy controls. This increased Th22 Cell percentage was positively correlated with the renal lesions of IgAN patients. Correspondingly, the expression of CCL20, CCL22, and CCL27 in renal tissue increased in IgAN patients. Tonsillitis exacerbated these overrepresentations of Th22 Cells and chemokines. It was found that HMC could produce CCL20, CCL22, and CCL27. The supernatant of HMC was chemotactic for Th22 Cells. This activity of HMC was stimulated by HS infection, whereas treatment of anti-CCL20, anti-CCL22, and anti-CCL27 antibodies partly blocked this chemoattractant effect of HMC. CONCLUSIONS Tonsil infection may aggravate the renal pathological lesions of IgAN by exacerbating Th22 Cell accumulation. Our data suggested a collaboration between HMC and Th22 Cells in IgAN with tonsillitis underlying the effects of CCL20, CCL22, and CCL27.
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Cordyceps sinensis may inhibit Th22 Cell chemotaxis to improve kidney function in lgA nephropathy.
American journal of translational research, 2018Co-Authors: Chenggen Xiao, Ping Xiao, Yusa Chen, Yang Wang, Guoqing Huang, Qiaoling ZhouAbstract:IgA nephropathy is the most common form of primary glomerulonephritis and an important cause of kidney failure. Cordyceps sinensis (CS) is a parasitic fungus that has a long history of use in Chinese medicine for the treatment of nephritis. Interleukin (IL)-22-producing helper T Cells (Th22 Cells) have been reported to be involved in lgA nephropathy. Th22 Cells link the immune response to tissue inflammation. To elucidate the possible efficacy and mechanisms by which CS counteracts nephritis, we established an IgA nephropathy model in 6-week-old female BALB/c mice. The mice were randomly separated into 3 groups, the normal control, IgA nephropathy and CS (5 mg/kg/d) treatment groups. The Th22 Cell frequencies and the relative pathological and cytokine changes were measured with flow cytometry, whereas the serum chemokine ligand 27 (CCL27) and IL-22 concentrations were detected with ELISA. The Th22 Cell frequency decreased after 1 month of CS therapy. Additionally, mesangial Cell proliferation decreased. Moreover, the chemokine receptor type 10 (CCR10), CCL27 and IL-22 expression levels were significantly reduced. In conclusion, CS may modulate the chemotaxis of Th22 Cells to suppress inflammatory responses in IgA nephropathy.
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Hemolytic streptococcus exacerbates tubulointerstitial lesions in IgA nephropathy through Th22 Cells chemotaxis and proliferation.
International journal of clinical and experimental pathology, 2017Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Zhou Xiao, Qiaoling ZhouAbstract:Background Tonsillitis and the immunopathologic response induced by it are essential for IgA nephropathy (IgAN) progression. In this study, we investigated the possible mechanism underlying Th22 Cells overrepresentation in tonsillitis related IgAN. Methods The distribution of Th22 Cells, and the expressions of CCR10 and CCL27 in IgAN patients were detected. Relationship between Th22 Cell chemotaxis and renal pathology lesions was determined. Transwell assay was performed to investigate the contribution of human tubular epithelial Cell (human kidney 2 Cell, HK2) to Th22 Cell chemokines underlying Hemolytic streptococcus (HS) infection. Additionally, the impacts of tubular inflammatory on Th22 Cell proliferation were investigated. Results Th22 Cells were significantly increased in IgAN patients. Accordingly, the expressions of CCR10 and CCL27 were increased in IgAN patients. Higher Th22 Cells, CCR10 and CCL27 correlated with severer tubulointerstitial lesions. It was observed that CCR10 and CCL27 were predominantly expressed on tubular epithelial Cells, and supernatants of HK2 were chemotactic for Th22 Cells. This activity of HK2 was partly blocked by anti-CCL27. Tonsillitis promoted the over expression of Th22 Cells, CCR10 and CCL27 in IgAN patients, and exacerbated their renal lesions. Inactivated HS aggravated Th22 Cell chemotaxis by promoting CCL27 secretion. Additionally, inactivated HS could accelerate Th22 Cell proliferation by promoting the secretion of IL-1, IL-6 and TNF-α. Conclusion Th22 Cell chemotaxis was involved in the pathogenesis of IgAN. Tubular epithelial Cells are vulnerable to Th22 Cell chemotaxis in IgAN. Tonsillitis may exacerbate the progression of IgAN by promoting Th22 Cell chemotaxis and Cell proliferation.
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Intrinsic renal Cells induce lymphocytosis of Th22 Cells from IgA nephropathy patients through B7-CTLA-4 and CCL-CCR pathways.
Molecular and cellular biochemistry, 2017Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Qiaoling Zhou, Chenggen XiaoAbstract:IgA nephropathy (IgAN), the most common glomerulonephritis, has an unclear pathogenesis. The role of Th22 Cells, which are intimately related to proteinuria and progression in IgAN, in mediating infection-related IgAN is unclear. This study aimed to characterize the association between intrinsic renal Cells (tubular epithelial Cells and mesangial Cells) and Th22 Cells in immune regulation of infection-related IgAN and to elucidate the impact of Th22 lymphocytosis; the proinflammatory cytokines IL-1, IL-6, and TNF-α; and CCL chemokines on kidney fibrosis. Hemolytic streptococcus infection induced an increase in IL-1, IL-6, and TNF-α, resulting in Th22 Cell differentiation from T lymphocytes obtained from patients with IgAN, and the CCL20-CCR6, CCL22-CCR4, and/or CCL27-CCR10 axes facilitated Th22 Cell chemotaxis. The increased amount of Th22 Cells caused an increase in TGF-β1 levels, and anti-CD80, anti-CD86, and CTLA-4Ig treatment reduced TGF-β1 levels by inhibiting Th22 lymphocytosis and secretion of cytokines and chemokines, thus potentially relieving kidney fibrosis. Our data suggest that Th22 Cells might be recruited into the kidneys via the CCL20-CCR6, CCL22-CCR4, and/or CCL27-CCR10 axes by mesangial Cells and tubular epithelial Cells in infection-related IgAN. Th22 Cell overrepresentation was attributed to stimulation of the B7-CTLA-4Ig antigen-presenting pathway and IL-1, IL-6, and TNF-α.
Lu Gan - One of the best experts on this subject based on the ideXlab platform.
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Acteoside relieves mesangial Cell injury by regulating Th22 Cell chemotaxis and proliferation in IgA nephropathy.
Renal failure, 2018Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Huimin Luo, Qiaoling ZhouAbstract:The existing therapies of IgA nephropathy are unsatisfying. Acteoside, the main component of Rehmannia glutinosa with anti-inflammatory and anti-immune effects, can improve urinary protein excretion and immune disorder. Th22 Cell is involved in IgA nephropathy progression. This study was determined to explore the effect of acteoside on mesangial injury underlying Th22 Cell disorder in IgA nephropathy. Serum Th22 Cells and urine total protein of patients with IgA nephropathy were measured before and after six months treatment of Rehmannia glutinosa acteoside or valsartan. Chemotactic assay and co-culture assay were performed to investigate the effect of acteoside on Th22 Cell chemotaxis and differentiation. The expression of CCL20, CCL22 and CCL27 were analyzed. To explore the effect of acteoside on mesangial Cell injury induced by inflammation, IL-1, IL-6, TNF-α and TGF-β1 were tested. Results showed that the proteinuria and Th22 lymphocytosis of patients with IgA nephropathy significantly improved after combination treatment of Rehmannia glutinosa acteoside and valsartan, compared with valsartan monotherapy. In vitro study further demonstrated that acteoside inhibit Th22 Cell chemotaxis by suppressing the production of Th22 Cell attractive chemokines, i.e., CCL20, CCL22 and CCL27. In addition, acteoside inhibited the Th22 Cell proliferation. Co-culture assay proved that acteoside could relieve the overexpression of pro-inflammatory cytokines, and prevent the synthesis of TGF-β1. TGF-β1 level in mesangial Cells was positively correlated with the Th22 Cell. This research demonstrated that acteoside can alleviate mesangial Cell inflammatory injury by modulating Th22 lymphocytes chemotaxis and proliferation.
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Tonsillitis exacerbates renal injury in IgA nephropathy through promoting Th22 Cells chemotaxis.
International urology and nephrology, 2018Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Huiming Luo, Fengmin Shao, Qiaoling ZhouAbstract:BACKGROUND Tonsillitis can promote the progression of IgA nephropathy (IgAN) by aggravating immunopathologic response. Th22 Cell disorder is involved in the pathogenesis of IgAN with tonsillitis. This study was determined to explore the possible mechanism of IgAN with tonsillitis underlying Th22 Cell chemotaxis response to the effect of CCL20, CCL22, and CCL27. METHODS This research was conducted on 65 subjects including 16 healthy controls (HC group), 5 patients with renal carcinoma (HTC group) and 44 patients with IgAN between 2015 and 2016. According to clinical symptoms and results of throat swab culture, patients with IgAN were divided into two groups: IgAN with tonsillitis (IgAN + tonsillitis, n = 14) and IgAN patients without tonsillitis (IgAN, n = 30). Distribution of Th22 Cells in IgAN patients was determined. The expression of CCL20, CCL22, and CCL27 in both peripheral blood and kidneys of IgAN patients was investigated. Severity of pathological lesions in IgAN patients was analyzed. Coculture assay and transwell assay were performed to explore the impacts of human mesangial Cells (HMC) on Th22 Cell chemotaxis and Th22 Cell local accumulation under hemolytic streptococcus (HS) infection. RESULTS Th22 Cell percentages in IgAN patients increased compared with healthy controls. This increased Th22 Cell percentage was positively correlated with the renal lesions of IgAN patients. Correspondingly, the expression of CCL20, CCL22, and CCL27 in renal tissue increased in IgAN patients. Tonsillitis exacerbated these overrepresentations of Th22 Cells and chemokines. It was found that HMC could produce CCL20, CCL22, and CCL27. The supernatant of HMC was chemotactic for Th22 Cells. This activity of HMC was stimulated by HS infection, whereas treatment of anti-CCL20, anti-CCL22, and anti-CCL27 antibodies partly blocked this chemoattractant effect of HMC. CONCLUSIONS Tonsil infection may aggravate the renal pathological lesions of IgAN by exacerbating Th22 Cell accumulation. Our data suggested a collaboration between HMC and Th22 Cells in IgAN with tonsillitis underlying the effects of CCL20, CCL22, and CCL27.
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Hemolytic streptococcus exacerbates tubulointerstitial lesions in IgA nephropathy through Th22 Cells chemotaxis and proliferation.
International journal of clinical and experimental pathology, 2017Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Zhou Xiao, Qiaoling ZhouAbstract:Background Tonsillitis and the immunopathologic response induced by it are essential for IgA nephropathy (IgAN) progression. In this study, we investigated the possible mechanism underlying Th22 Cells overrepresentation in tonsillitis related IgAN. Methods The distribution of Th22 Cells, and the expressions of CCR10 and CCL27 in IgAN patients were detected. Relationship between Th22 Cell chemotaxis and renal pathology lesions was determined. Transwell assay was performed to investigate the contribution of human tubular epithelial Cell (human kidney 2 Cell, HK2) to Th22 Cell chemokines underlying Hemolytic streptococcus (HS) infection. Additionally, the impacts of tubular inflammatory on Th22 Cell proliferation were investigated. Results Th22 Cells were significantly increased in IgAN patients. Accordingly, the expressions of CCR10 and CCL27 were increased in IgAN patients. Higher Th22 Cells, CCR10 and CCL27 correlated with severer tubulointerstitial lesions. It was observed that CCR10 and CCL27 were predominantly expressed on tubular epithelial Cells, and supernatants of HK2 were chemotactic for Th22 Cells. This activity of HK2 was partly blocked by anti-CCL27. Tonsillitis promoted the over expression of Th22 Cells, CCR10 and CCL27 in IgAN patients, and exacerbated their renal lesions. Inactivated HS aggravated Th22 Cell chemotaxis by promoting CCL27 secretion. Additionally, inactivated HS could accelerate Th22 Cell proliferation by promoting the secretion of IL-1, IL-6 and TNF-α. Conclusion Th22 Cell chemotaxis was involved in the pathogenesis of IgAN. Tubular epithelial Cells are vulnerable to Th22 Cell chemotaxis in IgAN. Tonsillitis may exacerbate the progression of IgAN by promoting Th22 Cell chemotaxis and Cell proliferation.
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Intrinsic renal Cells induce lymphocytosis of Th22 Cells from IgA nephropathy patients through B7-CTLA-4 and CCL-CCR pathways.
Molecular and cellular biochemistry, 2017Co-Authors: Lu Gan, Mengyuan Zhu, Chen Chen, Ting Meng, Qiaoling Zhou, Chenggen XiaoAbstract:IgA nephropathy (IgAN), the most common glomerulonephritis, has an unclear pathogenesis. The role of Th22 Cells, which are intimately related to proteinuria and progression in IgAN, in mediating infection-related IgAN is unclear. This study aimed to characterize the association between intrinsic renal Cells (tubular epithelial Cells and mesangial Cells) and Th22 Cells in immune regulation of infection-related IgAN and to elucidate the impact of Th22 lymphocytosis; the proinflammatory cytokines IL-1, IL-6, and TNF-α; and CCL chemokines on kidney fibrosis. Hemolytic streptococcus infection induced an increase in IL-1, IL-6, and TNF-α, resulting in Th22 Cell differentiation from T lymphocytes obtained from patients with IgAN, and the CCL20-CCR6, CCL22-CCR4, and/or CCL27-CCR10 axes facilitated Th22 Cell chemotaxis. The increased amount of Th22 Cells caused an increase in TGF-β1 levels, and anti-CD80, anti-CD86, and CTLA-4Ig treatment reduced TGF-β1 levels by inhibiting Th22 lymphocytosis and secretion of cytokines and chemokines, thus potentially relieving kidney fibrosis. Our data suggest that Th22 Cells might be recruited into the kidneys via the CCL20-CCR6, CCL22-CCR4, and/or CCL27-CCR10 axes by mesangial Cells and tubular epithelial Cells in infection-related IgAN. Th22 Cell overrepresentation was attributed to stimulation of the B7-CTLA-4Ig antigen-presenting pathway and IL-1, IL-6, and TNF-α.
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Differentiation and recruitment of IL-22-producing helper T Cells in lgA nephropathy.
American journal of translational research, 2016Co-Authors: Chenggen Xiao, Mengyuan Zhu, Ting Meng, Qiaoling Zhou, Yong Zhong, Lu GanAbstract:IL-22-producing helper T Cells (Th22 Cells) have been reported to be involved in lgA nephropathy. However, the mechanisms underlying the differentiation and immune regulation of Th22 Cells in lgA nephropathy remain unknown. To elucidate the mechanisms by which Th22 Cells differentiate and are recruited into the kidney in lgA nephropathy, the distribution of Th22 Cells in both the kidney and blood was determined. Additionally, the impacts of proinflammatory cytokines and antigen presentation in the kidney on Th22 Cell differentiation were explored. Specifically, the chemoattractant activities of chemokines produced by the kidney for Th22 Cells were investigated. Th22 Cells were significantly higher both in the kidney and in the blood in lgA nephropathy mice. IL-1β, IL-6, IL-21 and/or TNF-a promoted Th22 Cells differentiation from CD4+ T Cells. It was observed that kidneys undergoing lgA nephropathy expressed CCL20, CCL22 and CCL27, and kidney supernatants were chemotactic for Th22 Cells. This activity was partially blocked by anti-CCL20, anti-CCL22, and anti-CCL27 antibodies, which also potentially improved renal lesions simultaneously. The overrepresentation of Th22 Cells in lgAN may be attributable to the actions of kidney chemokines and cytokines. Our data suggest a collaborative loop between the kidney and Th22 Cells in lgA nephropathy.
Nannan Han - One of the best experts on this subject based on the ideXlab platform.
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increased th17 and Th22 Cell percentages predict acute lung injury in patients with sepsis
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P < 0.05). They were significant factors affecting sepsis-induced ALI (P < 0.05). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR = 2.612) and the percentages of Th17 (OR = 2.211) and Th22 (OR = 3.230) Cells were independent risk factors for ALI. The area under the curve of Th22 Cells was 0.844 to predict ALI with a cutoff value of 6.81%. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by the Th22 Cell percentage were 78.72% and 89.13%, respectively. CONCLUSIONS Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with ALI and they may be used as biomarkers for early diagnosis of sepsis-induced ALI.
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Increased Th17 and Th22 Cell Percentages Predict Acute Lung Injury in Patients with Sepsis.
Lung, 2020Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:PURPOSE This study was conducted to investigate the percentages of Th22 and Th17 Cells in the peripheral blood of septic patients with and without acute lung injury (ALI) and their clinical significance. METHODS A total of 479 patients were divided into non-ALI and ALI groups. The percentages of Th22 and Th17 Cells and the levels of interleukin 22 (IL-22), 6 (IL-6), and 17 (IL-17) were determined. Receiver operating characteristic curve analysis was performed to assess the diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. RESULTS The lung injury prediction score (LIPS), IL-6, IL-17, and IL-22 levels and the percentages of Th17 and Th22 Cells were significantly higher in the ALI group (P
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Increased Th17 and Th22 Cell levels predict acute lung injury in patients with sepsis
2019Co-Authors: Liangtian Zhang, Nannan Han, Ke ZhangAbstract:Abstract Background: Acute lung injury (ALI) is one of the major complications of severe sepsis. This study was conducted to investigate the levels of Th22 and Th17 Cells in the peripheral blood septic patients with ALI and their clinical significance. Results: A total of 479 septic patients admitted between January 2013 to January 2018 were divided into non-ALI (n = 377) and ALI groups (n = 102) based on the presence or absence of ALI. The levels of Th22 and Th17 Cells, interleukin 22 (IL-22), 6 (IL-6) and 17 (IL-17) were determined. Receiver operating characteristic curve (ROC) analysis was performed to assess the early diagnostic value of Th22 and Th17 Cells to predict sepsis-induced ALI. The lung injury prediction score (LIPS), IL-6, IL-17, IL-22, and levels of Th17 and Th-22 Cells were 9.13, 14.02 ng/L, 13.06 ng/L, 22.90 ng/L, 8.80% and 7.40%, respectively, in the ALI patients and were significantly higher in the ALI group than in non-ALI group (P < 0.05). Pearson correlation analysis showed that LIPS, IL-17, IL-22, Th17 Cells and Th22 Cells were significant factors affecting sepsis-induced ALI (P < 0.05). The correlation analysis showed that the levels of Th22 Cells in the peripheral blood of septic patients with ALI were positively correlated with LIPS, IL-22 and the levels of Th17 Cells (P < 0.05), and the levels of Th17 Cells were positively correlated with LIPS and IL-17 (P < 0.01). Multivariate logistic regression analysis showed that the LIPS (OR = 1.130), IL-17 (OR = 1.982), IL-22 (OR =2.612) and levels of Th17 (OR = 2.211) and Th22 (OR =3.230) Cells were independent risk factor for ALI. The area under the curve of Th22 Cells was 0.844 with a cutoff value of 6.81% to predict ALI. The sensitivity and specificity for early diagnosis of sepsis-induced ALI by Th22 Cells were 78.72% and 89.13% respectively, which were better but statistically similar as compared with Th17 Cells (P > 0.05). Conclusions: The levels of Th22 and Th17 Cells in peripheral blood are significantly increased in septic patients with induced ALI, and may be used for early diagnose of sepsis-induced ALI.