The Experts below are selected from a list of 205059 Experts worldwide ranked by ideXlab platform

Limin Xing - One of the best experts on this subject based on the ideXlab platform.

  • circulating cd4 cd25 regulatory t Cell and Th3 Cell of the patients with myelodysplastic syndromes
    Blood, 2007
    Co-Authors: Zonghong Shao, Huaquan Wang, Limin Xing
    Abstract:

    Abstract Objective To investigate the quantitive and qualitive changes of peripheral blood CD4+CD25+ regulatory T Cells and Th3 Cells of the patients with myelodysplastic syndromes. Methods Whole peripheral blood samples of 40 MDS cases and 19 nromal control were assayed by flow cytometer for detecting the numbers of CD4+CD25+ regulatory T Cell and CD3+CD4+TGFβ+ T Cell(Th3). Expression of FOXP3 and TGF-β of peripheral blood mononuclear Cell(PBMNC) were analysed by reverse transcriptase-polymerase chain reaction(RT-PCR). Commercial ELISA kits were used to measure plasma concentrations of TGF-β 1 according to the manufacturer's instructions. Results. The percentage and number of CD4+CD25+ regulatory T Cells in the CD4+T Cell population were significantly higher in the peripheral blood of MDS patients than healthy controls[(21.5±2.2% vs 8.3±1.9%; P Conclusion The CD4+CD25 + regulatory T Cells, Th3 Cells and their inhibitive function increased in MDS, which might contribute to MDS clone escaping from immunosurveillance and gainning immunol privilege.

Zonghong Shao - One of the best experts on this subject based on the ideXlab platform.

  • circulating cd4 cd25 regulatory t Cell and Th3 Cell of the patients with myelodysplastic syndromes
    Blood, 2007
    Co-Authors: Zonghong Shao, Huaquan Wang, Limin Xing
    Abstract:

    Abstract Objective To investigate the quantitive and qualitive changes of peripheral blood CD4+CD25+ regulatory T Cells and Th3 Cells of the patients with myelodysplastic syndromes. Methods Whole peripheral blood samples of 40 MDS cases and 19 nromal control were assayed by flow cytometer for detecting the numbers of CD4+CD25+ regulatory T Cell and CD3+CD4+TGFβ+ T Cell(Th3). Expression of FOXP3 and TGF-β of peripheral blood mononuclear Cell(PBMNC) were analysed by reverse transcriptase-polymerase chain reaction(RT-PCR). Commercial ELISA kits were used to measure plasma concentrations of TGF-β 1 according to the manufacturer's instructions. Results. The percentage and number of CD4+CD25+ regulatory T Cells in the CD4+T Cell population were significantly higher in the peripheral blood of MDS patients than healthy controls[(21.5±2.2% vs 8.3±1.9%; P Conclusion The CD4+CD25 + regulatory T Cells, Th3 Cells and their inhibitive function increased in MDS, which might contribute to MDS clone escaping from immunosurveillance and gainning immunol privilege.

Huaquan Wang - One of the best experts on this subject based on the ideXlab platform.

  • circulating cd4 cd25 regulatory t Cell and Th3 Cell of the patients with myelodysplastic syndromes
    Blood, 2007
    Co-Authors: Zonghong Shao, Huaquan Wang, Limin Xing
    Abstract:

    Abstract Objective To investigate the quantitive and qualitive changes of peripheral blood CD4+CD25+ regulatory T Cells and Th3 Cells of the patients with myelodysplastic syndromes. Methods Whole peripheral blood samples of 40 MDS cases and 19 nromal control were assayed by flow cytometer for detecting the numbers of CD4+CD25+ regulatory T Cell and CD3+CD4+TGFβ+ T Cell(Th3). Expression of FOXP3 and TGF-β of peripheral blood mononuclear Cell(PBMNC) were analysed by reverse transcriptase-polymerase chain reaction(RT-PCR). Commercial ELISA kits were used to measure plasma concentrations of TGF-β 1 according to the manufacturer's instructions. Results. The percentage and number of CD4+CD25+ regulatory T Cells in the CD4+T Cell population were significantly higher in the peripheral blood of MDS patients than healthy controls[(21.5±2.2% vs 8.3±1.9%; P Conclusion The CD4+CD25 + regulatory T Cells, Th3 Cells and their inhibitive function increased in MDS, which might contribute to MDS clone escaping from immunosurveillance and gainning immunol privilege.

James J Pestka - One of the best experts on this subject based on the ideXlab platform.

  • lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic Cells
    Journal of Immunology, 2002
    Co-Authors: Hanne Risager Christensen, Hanne Frokiaer, James J Pestka
    Abstract:

    Dendritic Cells (DC) play a pivotal immunoregulatory role in the Th1, Th2, and Th3 Cell balance and are present throughout the gastrointestinal tract. Thus, DC may be targets for modulation by gut microbes, including ingested probiotics. In the present study, we tested the hypothesis that species of Lactobacillus, important members of the gut flora, differentially activate DC. Bone marrow-derived murine DC were exposed to various lethally irradiated Lactobacillus spp. and resultant culture supernatants were analyzed for IL-6, IL-10, IL-12, and TNF-alpha. Substantial differences were found among strains in the capacity to induce IL-12 and TNF-alpha production in the DC. Similar but less pronounced differences were observed among lactobacilli in the induction of IL-6 and IL-10. Although all strains up-regulated surface MHC class II and B7-2 (CD86), which is indicative of DC maturation, those lactobacilli with greatest capacity to induce IL-12 were most effective. Remarkably, Lactobacillus reuteri DSM12246, a poor IL-12 inducer, inhibited IL-12, IL-6, and TNF-alpha induction by the otherwise strong cytokine inducer L. casei CHCC3139, while IL-10 production remained unaltered. In analogous fashion, L. reuteri reduced L. casei-induced up-regulation of B7-2. These results suggest that different species of Lactobacillus exert very different DC activation patterns and, furthermore, at least one species may be capable of inhibiting activities of other species in the genus. Thus, the potential exists for Th1/Th2/Th3-driving capacities of the gut DC to be modulated according to composition of gut microflora, including ingested probiotics.

Hanne Risager Christensen - One of the best experts on this subject based on the ideXlab platform.

  • lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic Cells
    Journal of Immunology, 2002
    Co-Authors: Hanne Risager Christensen, Hanne Frokiaer, James J Pestka
    Abstract:

    Dendritic Cells (DC) play a pivotal immunoregulatory role in the Th1, Th2, and Th3 Cell balance and are present throughout the gastrointestinal tract. Thus, DC may be targets for modulation by gut microbes, including ingested probiotics. In the present study, we tested the hypothesis that species of Lactobacillus, important members of the gut flora, differentially activate DC. Bone marrow-derived murine DC were exposed to various lethally irradiated Lactobacillus spp. and resultant culture supernatants were analyzed for IL-6, IL-10, IL-12, and TNF-alpha. Substantial differences were found among strains in the capacity to induce IL-12 and TNF-alpha production in the DC. Similar but less pronounced differences were observed among lactobacilli in the induction of IL-6 and IL-10. Although all strains up-regulated surface MHC class II and B7-2 (CD86), which is indicative of DC maturation, those lactobacilli with greatest capacity to induce IL-12 were most effective. Remarkably, Lactobacillus reuteri DSM12246, a poor IL-12 inducer, inhibited IL-12, IL-6, and TNF-alpha induction by the otherwise strong cytokine inducer L. casei CHCC3139, while IL-10 production remained unaltered. In analogous fashion, L. reuteri reduced L. casei-induced up-regulation of B7-2. These results suggest that different species of Lactobacillus exert very different DC activation patterns and, furthermore, at least one species may be capable of inhibiting activities of other species in the genus. Thus, the potential exists for Th1/Th2/Th3-driving capacities of the gut DC to be modulated according to composition of gut microflora, including ingested probiotics.