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Veronica S Di Stilio - One of the best experts on this subject based on the ideXlab platform.
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facilitating candidate gene discovery in an emerging model plant lineage transcriptomic and genomic resources for Thalictrum ranunculaceae
bioRxiv, 2020Co-Authors: Tatiana Arias, Diego Mauricio Riano Pachon, Veronica S Di StilioAbstract:The plant genus Thalictrum is a representative of the order Ranunculales (a sister lineage to all other Eudicots) with diverse floral morphologies, encompassing four sexual systems and two pollination modes. Previous studies suggest multiple transitions from insect to wind pollination within this genus, in association with polyploidy and unisexual flowers, but the underlying genes remain unknown. We generated a draft reference genome for Thalictrum thalictroides, a representative of a clade with ancestral floral traits (diploidy, hermaphroditism, and insect pollination) and a model for functional studies. To facilitate candidate gene discovery in flowers with different sexual and pollination systems we also generated floral transcriptomes of T. thalictroides and of wind-pollinated, andromonoecious (staminate and hermaphroditic flowers on the same plant) T. hernandezii. The T. thalictroides draft genome assembly consisted of 44,860 contigs (N50=12,761 bp. and 243 Mbp. total length) and contained 84.5% conserved embryophyte single-copy genes. Floral transcriptomes from Illumina sequencing and de novo assembly contained representatives of most eukaryotic core genes (approximately 80%), with most of their genes falling into common orthologous groups (orthogroups). Simple Sequence Repeat (SSR) motifs were also identified, which together with the single-copy genes constitute a resource for population-level or phylogenetic studies. Finally, to validate the utility of these resources, putative candidate genes were identified for the different floral morphologies using stepwise dataset comparisons. In conclusion, we present genomic and transcriptomic resources for Thalictrum, including the first genome of T. thalictroides and potential candidate genes for flowers with distinct sexual and pollination systems.
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chloroplast primers for clade wide phylogenetic studies of Thalictrum
Applications in Plant Sciences, 2019Co-Authors: Diego F Moralesbriones, Veronica S Di Stilio, Tatiana Arias, David C TankAbstract:Premise Chloroplast primers were developed for phylogenetic and comparative studies in Thalictrum (Ranunculaceae). Methods and results We assembled and annotated the complete plastome sequence of T. thalictroides by combining multiple whole genome sequencing libraries. Using transcriptome-sequencing libraries, we also assembled a partial plastome of the related species T. hernandezii. From the newly assembled plastomes and one previously sequenced plastome, we designed and validated 28 primer pairs to target variable portions of the chloroplast genome in Thalictrum. Furthermore, we tested the validated primers in 62 species of Thalictrum. The total alignment length of the 28 regions was 15,268 bp with 2443 variable sites and 92% character occupancy. Conclusions The newly developed chloroplast primer pairs improve the phylogenetic resolution (bootstrap support and tree certainty) in Thalictum and will be a useful resource for future phylogenetic and evolutionary studies for species in the genus and in close relatives in Thalictroideae.
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scent matters differential contribution of scent to insect response in flowers with insect vs wind pollination traits
Annals of Botany, 2019Co-Authors: Theresa N Wang, Marie R Clifford, Jesus Martinezgomez, Jens C Johnson, Jeffrey A Riffell, Veronica S Di StilioAbstract:Background and Aims Growing experimental evidence that floral scent is a key contributor to pollinator attraction supports its investigation as a component of the suite of floral traits that result from pollinator-mediated selection. Yet, the fate of floral scent during the transition out of biotic into abiotic pollination has rarely been tested. In the case of wind pollination, this is due not only to its rarer incidence among flowering plants compared with insect pollination, but also to the scarcity of systems amenable to genus-level comparisons. Thalictrum (Ranunculaceae), with its multiple transitions from insect to wind pollination, offers a unique opportunity to test interspecific changes in floral fragrance and their potential impact on pollinator attraction. Methods First, the Thalictrum phylogeny was revised and the ancestral character state of pollination mode was reconstructed. Then, volatile organic compounds (VOCs) that comprise the scent bouquets of flowers from 11 phylogenetically representative wind- and insect-pollinated species were characterized and compared. Finally, to test the hypothesis that scent from insect-pollinated flowers elicits a significantly greater response from potential pollinators than that from wind-pollinated flowers, electroantennograms (EAGs) were performed on Bombus impatiens using whole flower extracts. Key Results Phylogenetic reconstruction of the pollination mode recovered 8-10 transitions to wind pollination from an ancestral insect pollination state and two reversals to insect pollination. Biochemical and multivariate analysis showed that compounds are distinct by species and only partially segregate with pollination mode, with no significant phylogenetic signal on scent composition. Floral VOCs from insect-pollinated Thalictrum elicited a larger antennal response from potential insect pollinators than those from wind-pollinated congeners. Conclusions An evolutionary scenario is proposed where an ancestral ability of floral fragrance to elicit an insect response through the presence of specific compounds was independently lost during the multiple evolutionary transitions to wind pollination in Thalictrum.
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gene duplication and transference of function in the paleoap3 lineage of floral organ identity genes
Frontiers in Plant Science, 2018Co-Authors: Kelsey D. Galimba, Jesus Martinezgomez, Veronica S Di StilioAbstract:The floral organ identity gene APETALA3 (AP3) is a MADS-box transcription factor involved in stamen and petal identity that belongs to the B-class of the ABC model of flower development. Thalictrum (Ranunculaceae), an emerging model in the non-core eudicots, has AP3 homologs derived from both ancient and recent gene duplications. Prior work has shown that petals have been lost repeatedly and independently in Ranunculaceae in correlation with the loss of a specific AP3 paralog, and Thalictrum represents one of these instances. The main goal of this study was to conduct a functional analysis of the three AP3 orthologs present in Thalictrum thalictroides, representing the paleoAP3 gene lineage, to determine the degree of redundancy versus divergence after gene duplication. Because Thalictrum lacks petals, and has lost the petal-specific AP3, we also asked whether heterotopic expression of the remaining AP3 genes contributes to the partial transference of petal function to the first whorl found in insect-pollinated species. To address these questions, we undertook functional characterization by virus induced gene silencing, protein-protein interaction and binding site analyses. Our results illustrate partial redundancy among Thalictrum AP3s, with deep conservation of B-class function in stamen identity and a novel role in ectopic petaloidy of sepals. Certain aspects of petal function of the lost AP3 locus have apparently been transferred to the other paralogs. A novel result is that the protein products interact not only with each other, but also as homodimers. Evidence presented here also suggests that expression of the different ThtAP3 paralogs is tightly integrated, with an apparent disruption of B function homeostasis upon silencing of one of the paralogs that codes for a truncated protein. To explain this result, we propose two testable alternative scenarios: that the truncated protein is a dominant negative mutant or that there is a compensational response as part of a back-up circuit. The evidence for promiscuous protein-protein interactions via yeast-two-hybrid combined with the detection of AP3 specific binding motifs in all B-class gene promoters provide partial support for these hypotheses.
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Presentation_1.PDF
2018Co-Authors: Kelsey D. Galimba, Jesús Martínez-gómez, Veronica S Di StilioAbstract:The floral organ identity gene APETALA3 (AP3) is a MADS-box transcription factor involved in stamen and petal identity that belongs to the B-class of the ABC model of flower development. Thalictrum (Ranunculaceae), an emerging model in the non-core eudicots, has AP3 homologs derived from both ancient and recent gene duplications. Prior work has shown that petals have been lost repeatedly and independently in Ranunculaceae in correlation with the loss of a specific AP3 paralog, and Thalictrum represents one of these instances. The main goal of this study was to conduct a functional analysis of the three AP3 orthologs present in Thalictrum thalictroides, representing the paleoAP3 gene lineage, to determine the degree of redundancy versus divergence after gene duplication. Because Thalictrum lacks petals, and has lost the petal-specific AP3, we also asked whether heterotopic expression of the remaining AP3 genes contributes to the partial transference of petal function to the first whorl found in insect-pollinated species. To address these questions, we undertook functional characterization by virus-induced gene silencing (VIGS), protein–protein interaction and binding site analyses. Our results illustrate partial redundancy among Thalictrum AP3s, with deep conservation of B-class function in stamen identity and a novel role in ectopic petaloidy of sepals. Certain aspects of petal function of the lost AP3 locus have apparently been transferred to the other paralogs. A novel result is that the protein products interact not only with each other, but also as homodimers. Evidence presented here also suggests that expression of the different ThtAP3 paralogs is tightly integrated, with an apparent disruption of B function homeostasis upon silencing of one of the paralogs that codes for a truncated protein. To explain this result, we propose two testable alternative scenarios: that the truncated protein is a dominant negative mutant or that there is a compensational response as part of a back-up circuit. The evidence for promiscuous protein–protein interactions via yeast two-hybrid combined with the detection of AP3 specific binding motifs in all B-class gene promoters provide partial support for these hypotheses.
Toshihiro Nohara - One of the best experts on this subject based on the ideXlab platform.
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dolabellane diterpene and three cycloartane glycosides from Thalictrum squarrosum
ChemInform, 2011Co-Authors: Hitoshi Yoshimitsu, Hiroyuki Miyashita, Makiko Nishida, Tomoko Mineno, Toshihiro NoharaAbstract:The plant Thalictrum squarrosum has been used in the traditional Chinese medicine as an antacid.
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dolabellane diterpene and three cycloartane glycosides from Thalictrum squarrosum
Chemical & Pharmaceutical Bulletin, 2010Co-Authors: Hitoshi Yoshimitsu, Hiroyuki Miyashita, Makiko Nishida, Tomoko Mineno, Toshihiro NoharaAbstract:A new dolabellane diterpene glycoside, named squoside A, and three new cycloartane glycosides, named squarrosides V, VI, and VII, have been isolated from the dried aerial parts of Thalictrum squarrosum (Ranunculaceae). Their structures were determined by two dimensional (2D) NMR spectroscopic analysis and chemical evidence.
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four new triterpene glycosides from Thalictrum squarrosum
ChemInform, 2000Co-Authors: Hitoshi Yoshimitsu, Makiko Nishida, Zhongzhi Qian, Zhenhuan Lei, Toshihiro NoharaAbstract:Four new triterpene glycosides were isolated from the dried aerial parts of Thalictrum squarrosum (Ranunculaceae). They were designated as squarroside I, being a cycloartane-type glycoside, and squarrosides II, III and IV, being oleanene-type glycosides. Their structures were established by using two dimensional (2D) NMR techniques.
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two new cycloartane glycosides from Thalictrum thunbergii d c
Tetrahedron Letters, 1998Co-Authors: Hitoshi Yoshimitsu, Makiko Nishida, Shoji Yahara, Toshihiro NoharaAbstract:Abstract Three new cycloartane glycosides possessing a five-membered ring, which is constructed by a C–C bond, at the side chain have been isolated from the aerial parts of Thalictrum thunbergii D.C. Their structures were determined by the use of 2D NMR techniques and chemical evidence.
Xiaodong Luo - One of the best experts on this subject based on the ideXlab platform.
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thalicfoetine a novel isoquinoline alkaloid with antibacterial activity from Thalictrum foetidum
Tetrahedron Letters, 2019Co-Authors: Caifeng Ding, Xujie Qin, Yaping Liu, Xinhua Wang, Xiaodong LuoAbstract:Abstract Thalicfoetine (1), the first O-bridged spirobenzylisoquinoline alkaloid possesses a spiro tetrahydropyridine-furanone core, was isolated from the roots of Thalictrum foetidum. Its structure was elucidated by extensive spectroscopic analyses and ECD calculation. Moreover, 1 exhibited antibacterial activity against Bacillus subtilis significantly with MIC value of 3.12 μg/mL.
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Hybrid isoquinolines from Thalictrum foetidum: a new type of aporphine inhibiting Staphylococcus aureus by combined mechanisms
Organic Chemistry Frontiers, 2019Co-Authors: Caifeng Ding, Xujie Qin, Yaping Liu, Rongping Zhang, Jing Yang, Zhi Dai, Ren Lai, Xiaodong LuoAbstract:Thalfoetines A–D (1–4), unique hybrid aporphine alkaloids with a C-7 aromatic unit formed by a new C–C bond linking two building blocks, were isolated from Thalictrum foetidum. Their structures were elucidated by extensive spectroscopic and computational analysis. They inhibited bacteria significantly, and compound 1 damaged the cell structure of Staphylococcus aureus and inhibited its DNA synthesis.
Hitoshi Yoshimitsu - One of the best experts on this subject based on the ideXlab platform.
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dolabellane diterpene and three cycloartane glycosides from Thalictrum squarrosum
ChemInform, 2011Co-Authors: Hitoshi Yoshimitsu, Hiroyuki Miyashita, Makiko Nishida, Tomoko Mineno, Toshihiro NoharaAbstract:The plant Thalictrum squarrosum has been used in the traditional Chinese medicine as an antacid.
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dolabellane diterpene and three cycloartane glycosides from Thalictrum squarrosum
Chemical & Pharmaceutical Bulletin, 2010Co-Authors: Hitoshi Yoshimitsu, Hiroyuki Miyashita, Makiko Nishida, Tomoko Mineno, Toshihiro NoharaAbstract:A new dolabellane diterpene glycoside, named squoside A, and three new cycloartane glycosides, named squarrosides V, VI, and VII, have been isolated from the dried aerial parts of Thalictrum squarrosum (Ranunculaceae). Their structures were determined by two dimensional (2D) NMR spectroscopic analysis and chemical evidence.
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four new triterpene glycosides from Thalictrum squarrosum
ChemInform, 2000Co-Authors: Hitoshi Yoshimitsu, Makiko Nishida, Zhongzhi Qian, Zhenhuan Lei, Toshihiro NoharaAbstract:Four new triterpene glycosides were isolated from the dried aerial parts of Thalictrum squarrosum (Ranunculaceae). They were designated as squarroside I, being a cycloartane-type glycoside, and squarrosides II, III and IV, being oleanene-type glycosides. Their structures were established by using two dimensional (2D) NMR techniques.
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two new cycloartane glycosides from Thalictrum thunbergii d c
Tetrahedron Letters, 1998Co-Authors: Hitoshi Yoshimitsu, Makiko Nishida, Shoji Yahara, Toshihiro NoharaAbstract:Abstract Three new cycloartane glycosides possessing a five-membered ring, which is constructed by a C–C bond, at the side chain have been isolated from the aerial parts of Thalictrum thunbergii D.C. Their structures were determined by the use of 2D NMR techniques and chemical evidence.
Caifeng Ding - One of the best experts on this subject based on the ideXlab platform.
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thalicfoetine a novel isoquinoline alkaloid with antibacterial activity from Thalictrum foetidum
Tetrahedron Letters, 2019Co-Authors: Caifeng Ding, Xujie Qin, Yaping Liu, Xinhua Wang, Xiaodong LuoAbstract:Abstract Thalicfoetine (1), the first O-bridged spirobenzylisoquinoline alkaloid possesses a spiro tetrahydropyridine-furanone core, was isolated from the roots of Thalictrum foetidum. Its structure was elucidated by extensive spectroscopic analyses and ECD calculation. Moreover, 1 exhibited antibacterial activity against Bacillus subtilis significantly with MIC value of 3.12 μg/mL.
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Hybrid isoquinolines from Thalictrum foetidum: a new type of aporphine inhibiting Staphylococcus aureus by combined mechanisms
Organic Chemistry Frontiers, 2019Co-Authors: Caifeng Ding, Xujie Qin, Yaping Liu, Rongping Zhang, Jing Yang, Zhi Dai, Ren Lai, Xiaodong LuoAbstract:Thalfoetines A–D (1–4), unique hybrid aporphine alkaloids with a C-7 aromatic unit formed by a new C–C bond linking two building blocks, were isolated from Thalictrum foetidum. Their structures were elucidated by extensive spectroscopic and computational analysis. They inhibited bacteria significantly, and compound 1 damaged the cell structure of Staphylococcus aureus and inhibited its DNA synthesis.
