The Experts below are selected from a list of 446178 Experts worldwide ranked by ideXlab platform
Maria L. Marco - One of the best experts on this subject based on the ideXlab platform.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Maria L. MarcoAbstract:Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572 bp chromosome and a 214,319 bp mega-plasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and Multi-Locus Sequence Typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically-relevant antibiotics. This organism also lacks or contains non-functional copies of enterococcal virulence genes including acm, cyl, the ebp operon , esp, gelE, hyl, and IS 16 , and associated phenotypes. It does contain scm, sagA, efaA, and the pilA, although these genes were either not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Eun Bae Kim, Maria L. MarcoAbstract:ABSTRACT Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572-bp chromosome and a 214,319-bp megaplasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and multilocus sequence typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically relevant antibiotics. This organism also lacks, or contains nonfunctional copies of, enterococcal virulence genes including acm, cyl, the ebp operon, esp, gelE, hyl, IS16, and associated phenotypes. It does contain scm, sagA, efaA, and pilA, although either these genes were not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
Lauren M Kopit - One of the best experts on this subject based on the ideXlab platform.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Maria L. MarcoAbstract:Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572 bp chromosome and a 214,319 bp mega-plasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and Multi-Locus Sequence Typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically-relevant antibiotics. This organism also lacks or contains non-functional copies of enterococcal virulence genes including acm, cyl, the ebp operon , esp, gelE, hyl, and IS 16 , and associated phenotypes. It does contain scm, sagA, efaA, and the pilA, although these genes were either not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Eun Bae Kim, Maria L. MarcoAbstract:ABSTRACT Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572-bp chromosome and a 214,319-bp megaplasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and multilocus sequence typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically relevant antibiotics. This organism also lacks, or contains nonfunctional copies of, enterococcal virulence genes including acm, cyl, the ebp operon, esp, gelE, hyl, IS16, and associated phenotypes. It does contain scm, sagA, efaA, and pilA, although either these genes were not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
Tao Hou - One of the best experts on this subject based on the ideXlab platform.
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synthesis of carbon nitride nanotubes with the c3n4 stoichiometry via a benzene Thermal Process at low temperatures
Chemical Communications, 2004Co-Authors: Qixun Guo, Yi Xie, Xinjun Wang, Shuyuan Zhang, Tao HouAbstract:In this communication, we first report the direct synthesis of high-quality carbon nitride nanotubes (CNNTs) with inner diameters of 50–100 nm and wall thicknesses of 20–50 nm with the C3N4 stoichiometry on a high-yield of 40% via a simple benzene-Thermal Process involving the reaction of C3N3Cl3 with NaN3 in a Teflon-lined autoclave at 220 °C without using any catalyst or template.
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synthesis of carbon nitride nanotubes with the c 3 n 4 stoichiometry via a benzene Thermal Process at low temperatures
Chemical Communications, 2004Co-Authors: Qixun Guo, Yi Xie, Xinjun Wang, Shuyuan Zhang, Tao HouAbstract:In this communication, we first report the direct synthesis of high-quality carbon nitride nanotubes (CNNTs) with inner diameters of 50–100 nm and wall thicknesses of 20–50 nm with the C3N4 stoichiometry on a high-yield of 40% via a simple benzene-Thermal Process involving the reaction of C3N3Cl3 with NaN3 in a Teflon-lined autoclave at 220 °C without using any catalyst or template.
Eun Bae Kim - One of the best experts on this subject based on the ideXlab platform.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Eun Bae Kim, Maria L. MarcoAbstract:ABSTRACT Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572-bp chromosome and a 214,319-bp megaplasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and multilocus sequence typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically relevant antibiotics. This organism also lacks, or contains nonfunctional copies of, enterococcal virulence genes including acm, cyl, the ebp operon, esp, gelE, hyl, IS16, and associated phenotypes. It does contain scm, sagA, efaA, and pilA, although either these genes were not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
Rolnald J. Siezen - One of the best experts on this subject based on the ideXlab platform.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Maria L. MarcoAbstract:Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572 bp chromosome and a 214,319 bp mega-plasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and Multi-Locus Sequence Typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically-relevant antibiotics. This organism also lacks or contains non-functional copies of enterococcal virulence genes including acm, cyl, the ebp operon , esp, gelE, hyl, and IS 16 , and associated phenotypes. It does contain scm, sagA, efaA, and the pilA, although these genes were either not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
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safety of the surrogate microorganism enterococcus faecium nrrl b 2354 for use in Thermal Process validation
Applied and Environmental Microbiology, 2014Co-Authors: Lauren M Kopit, Linda J Harris, Rolnald J. Siezen, Eun Bae Kim, Maria L. MarcoAbstract:ABSTRACT Enterococcus faecium NRRL B-2354 is a surrogate microorganism used in place of pathogens for validation of Thermal Processing technologies and systems. We evaluated the safety of strain NRRL B-2354 based on its genomic and functional characteristics. The genome of E. faecium NRRL B-2354 was sequenced and found to comprise a 2,635,572-bp chromosome and a 214,319-bp megaplasmid. A total of 2,639 coding sequences were identified, including 45 genes unique to this strain. Hierarchical clustering of the NRRL B-2354 genome with 126 other E. faecium genomes as well as pbp5 locus comparisons and multilocus sequence typing (MLST) showed that the genotype of this strain is most similar to commensal, or community-associated, strains of this species. E. faecium NRRL B-2354 lacks antibiotic resistance genes, and both NRRL B-2354 and its clonal relative ATCC 8459 are sensitive to clinically relevant antibiotics. This organism also lacks, or contains nonfunctional copies of, enterococcal virulence genes including acm, cyl, the ebp operon, esp, gelE, hyl, IS16, and associated phenotypes. It does contain scm, sagA, efaA, and pilA, although either these genes were not expressed or their roles in enterococcal virulence are not well understood. Compared with the clinical strains TX0082 and 1,231,502, E. faecium NRRL B-2354 was more resistant to acidic conditions (pH 2.4) and high temperatures (60°C) and was able to grow in 8% ethanol. These findings support the continued use of E. faecium NRRL B-2354 in Thermal Process validation of food products.
