The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform
Guangming Zeng - One of the best experts on this subject based on the ideXlab platform.
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The isolation, identification of sludge-lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge.
Environmental technology, 2020Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65 degrees C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after inoculation with Bacillus Thermophilic Bacteria AT07-1, which was 10.24% higher than the non-inoculated system, and which could meet the standard of sludge stability suggested by the US Environmental Protection Agency.
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the isolation identification of sludge lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge
Environmental Technology, 2012Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65°C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after i...
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Enhanced Hydrogen Production from Sewage Sludge Pretreated by Thermophilic Bacteria
Energy & Fuels, 2010Co-Authors: Xiaoming Li, Guangming Zeng, Qi YangAbstract:Thermophilic Bacteria pretreated sludge has drawn more interest for hydrogen production, which was more economical (needs less energy), pollution-free (adding a reagent is not needed), and efficient than physico-chemistry pretreated sludge. Comparative studies between Thermophilic Bacteria (Bacillus sp. AT07-1) and 65 °C heat pretreated sewage sludge have been conducted for hydrogen production. It was concluded that using Thermophilic Bacteria pretreated sludge for hydrogen production (batch of TB) would produce the highest hydrogen yield of 16.3 mL/g of volatile solid (VS), and it increased by 12.9, 26.4, and 52.3% compared to T + HB (65 °C heat pretreated sludge inoculated with Enterococcus sp. LG1), T (65 °C heat pretreated sludge), and TB + HB (Thermophilic Bacteria pretreated sludge inoculated with Enterococcus sp. LG1), respectively. The lag time of TB was only 3 h. The hydrogen concentration in the gas phase of TB + HB and T + HB was 66.2 and 67%, respectively, higher than that of TB and T. These r...
Ahmet Adiguzel - One of the best experts on this subject based on the ideXlab platform.
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Isolation and Characterization of Thermophilic Bacteria from Geothermal Areas in Turkey and Preliminary Research on Biotechnologically Important Enzyme Production
Geomicrobiology Journal, 2016Co-Authors: Mustafa Ozkan Baltaci, Berna Genc, Sumeyya Arslan, Gulsah Adiguzel, Ahmet AdiguzelAbstract:ABSTRACTIn this study, the isolation, identification and characterization of the Thermophilic Bacteria from different hot springs in Turkey were carried out by conventional (morphological, physiological and biochemical tests) and molecular methods (fatty acid methyl esters, GTG5-PCR and 16S rRNA sequencing). These Thermophilic Bacteria were then tested for their capability to produce enzymes such as lipase, protease, amylase and cellulase. O20 strain is a novel species according to identification studies. All of its isolates were capable of producing industrially valuable enzymes based on screening. In fact, most of them could produce at least two of these enzymes.
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identification and characterization of Thermophilic Bacteria isolated from hot springs in turkey
Journal of Microbiological Methods, 2009Co-Authors: Ahmet Adiguzel, Medine Gulluce, Hakan Ozkan, Ozlem Baris, Kadriye Inan, Fikrettin SahinAbstract:article i nfo The present study was conducted to identify and characterize the Thermophilic Bacteria isolated from various hot springs in Turkey by using phenotypic and genotypic methods including fatty acid methyl ester and rep- PCR profilings, and 16S rRNA sequencing. The data of fatty acid analysis showed the presence of 17 different fatty acids in 15 Bacterial strains examined in this study. Six fatty acids, 15:0 iso, 15:0 anteiso, 16:0, 16:0 iso, 17:0 iso, and 17:0 anteiso, were present in all strains. The Bacterial strains were classified into three phenotypic groups based on fatty acid profiles which were confirmed by genotypic methods such as 16S rRNA sequence analysis and rep-PCR genomic fingerprint profiles. After evaluating several primer sets targeting the repetitive DNA elements of REP, ERIC, BOX and (GTG)5, the (GTG)5 and BOXA1R primers were found to be the most reliable technique for identification and taxonomic characterization of Thermophilic Bacteria in the genera of Geobacillus, Anoxybacillus and Bacillus spp. Therefore, rep-PCR fingerprinting using the (GTG)5 and BOXA1R primers can be considered as a promising genotypic tool for the identification and characterization of Thermophilic Bacteria from species to strain level.
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characterization of Thermophilic Bacteria using surface enhanced raman scattering
Applied Spectroscopy, 2008Co-Authors: Mustafa Culha, Ahmet Adiguzel, Muge M Yazici, Mehmet Kahraman, Fikrettin Slahin, Medine GulluceAbstract:Surface-enhanced Raman scattering (SERS) can provide molecular-level information about the molecules and molecular structures in the vicinity of nanostructured noble metal surfaces such as gold and silver. The three Thermophilic Bacteria Bacillus licheniformis, Geobacillus stearothermophilus, and Geobacillus pallidus, a Gram-negative bacterium E. coli, and a Gram-positive bacterium B. megaterium are comparatively characterized using SERS. The SERS spectra of Thermophilic Bacteria are similar, while they show significant differences compared to E. coli and B. megaterium. The findings indicate that a higher number of thiol residues and possible S–S bridges are present in the cell wall structure of Thermophilic Bacteria, providing their stability at elevated temperatures. Incubating the Thermophilic Bacteria with colloidal silver suspension at longer times improved the Bacteria–silver nanoparticle interaction kinetics, while increased temperature does not have a pronounced effect on spectral features. A tentative assignment of the SERS bands was attempted for Thermophilic Bacteria. The results indicate that SERS can be a useful tool to study Bacterial cell wall molecular differences.
Qi Yang - One of the best experts on this subject based on the ideXlab platform.
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The isolation, identification of sludge-lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge.
Environmental technology, 2020Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65 degrees C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after inoculation with Bacillus Thermophilic Bacteria AT07-1, which was 10.24% higher than the non-inoculated system, and which could meet the standard of sludge stability suggested by the US Environmental Protection Agency.
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the isolation identification of sludge lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge
Environmental Technology, 2012Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65°C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after i...
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Enhanced Hydrogen Production from Sewage Sludge Pretreated by Thermophilic Bacteria
Energy & Fuels, 2010Co-Authors: Xiaoming Li, Guangming Zeng, Qi YangAbstract:Thermophilic Bacteria pretreated sludge has drawn more interest for hydrogen production, which was more economical (needs less energy), pollution-free (adding a reagent is not needed), and efficient than physico-chemistry pretreated sludge. Comparative studies between Thermophilic Bacteria (Bacillus sp. AT07-1) and 65 °C heat pretreated sewage sludge have been conducted for hydrogen production. It was concluded that using Thermophilic Bacteria pretreated sludge for hydrogen production (batch of TB) would produce the highest hydrogen yield of 16.3 mL/g of volatile solid (VS), and it increased by 12.9, 26.4, and 52.3% compared to T + HB (65 °C heat pretreated sludge inoculated with Enterococcus sp. LG1), T (65 °C heat pretreated sludge), and TB + HB (Thermophilic Bacteria pretreated sludge inoculated with Enterococcus sp. LG1), respectively. The lag time of TB was only 3 h. The hydrogen concentration in the gas phase of TB + HB and T + HB was 66.2 and 67%, respectively, higher than that of TB and T. These r...
Ibrahim M. Banat - One of the best experts on this subject based on the ideXlab platform.
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The Use Of Thermophilic Bacteria InAccelerated Hydrocarbon Bioremediation
Environmental Problems in Coastal Regions VI, 2006Co-Authors: Amedea Perfumo, Ibrahim M. Banat, Roger MarchantAbstract:Successful bioremediation of hydrocarbon contamination in terrestrial as well as aquatic ecosystems is generally limited by the low bioavailability of hydrophobic pollutants. Considerably higher mass transfer rates and hydrocarbon solubilities can be obtained at higher temperatures, but so far approaches to bioremediation at increased temperature have hardly been investigated. The biotechnological significance and the benefits of the use of Thermophilic Bacteria in enhancing hydrocarbon removal under different environmental conditions were investigated. Expression of the alkane mono-oxygenase alkB gene responsible for the initial step in the degradation of alkanes in soil geobacilli was found to be induced by the presence of such a substrate at high temperature, and this explains the increased degradation rates achieved in hexadecane-contaminated microcosms incubated at 60°C compared to mesophilic conditions. Additional benefits of increasing hydrocarbon degradation also occur when Thermophilic Bacteria capable of producing biosurfactants which act as solubilising agents in such systems are introduced. We conclude that thermally accelerated bioremediation may be an effective technology for hydrocarbon contaminated soil bioremediation.
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Distribution and molecular investigation of highly Thermophilic Bacteria associated with cool soil environments.
Biochemical Society transactions, 2004Co-Authors: T.j. Rahman, Roger Marchant, Ibrahim M. BanatAbstract:In this study, both molecular and culture-based methods were used to characterize Thermophilic Bacteria associated with the subsurface soil environment in Northern Ireland. A total of 53 Thermophilic, aerobic, sporulating and non-sporulating Bacteria were isolated from subsurface soil samples obtained from two sites. They were screened by amplified ribosomal DNA restriction analysis prior to 16 S rRNA gene sequencing. The majority of the sequences were associated with Geobacillus thermoleovorans (50%) and Geobacillus caldoxylosilyticus (34.6%). Isolates F10, F20 and Tf exhibited only 93% similarity with Geobacillus toebii strain F70. Hence they may represent a new species of the genus Geobacillus.
Xiaoming Li - One of the best experts on this subject based on the ideXlab platform.
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The isolation, identification of sludge-lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge.
Environmental technology, 2020Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65 degrees C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after inoculation with Bacillus Thermophilic Bacteria AT07-1, which was 10.24% higher than the non-inoculated system, and which could meet the standard of sludge stability suggested by the US Environmental Protection Agency.
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the isolation identification of sludge lysing Thermophilic Bacteria and its utilization in solubilization for excess sludge
Environmental Technology, 2012Co-Authors: Ying Tang, Xiaoming Li, Qi Yang, Yong-lin Yang, Dong-bo Wang, Guangming ZengAbstract:A novel strain of Thermophilic Bacteria with a highly efficient sludge dissolution performance was isolated from garden soil at 65°C in this study. The colony morphology, physiological and biochemical characteristics of the strain were investigated. The results showed that the strain was Gram-positive, small rod-shaped, sporulating and secreted extracellular enzymes (protease and amylase). The 16S rDNA analysis demonstrated that this strain had not been previously reported. Therefore, it was labelled Bacillus Thermophilic Bacteria AT07-1 (registration number: FJ231108). To evaluate its capability for excess sludge solubilization, a pure culture of the strain was used in sludge solubilization tests; an enhanced solubilization process was subsequently obtained. After 36 h digestion, the protease activity in the inoculated system reached 0.37 U/ml, an increase of 0.16 U/ml compared with the non-inoculated system (0.21 U/ml). The solubilization rate for volatile suspended solids reached 46.45% in 48 h after i...
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Enhanced Hydrogen Production from Sewage Sludge Pretreated by Thermophilic Bacteria
Energy & Fuels, 2010Co-Authors: Xiaoming Li, Guangming Zeng, Qi YangAbstract:Thermophilic Bacteria pretreated sludge has drawn more interest for hydrogen production, which was more economical (needs less energy), pollution-free (adding a reagent is not needed), and efficient than physico-chemistry pretreated sludge. Comparative studies between Thermophilic Bacteria (Bacillus sp. AT07-1) and 65 °C heat pretreated sewage sludge have been conducted for hydrogen production. It was concluded that using Thermophilic Bacteria pretreated sludge for hydrogen production (batch of TB) would produce the highest hydrogen yield of 16.3 mL/g of volatile solid (VS), and it increased by 12.9, 26.4, and 52.3% compared to T + HB (65 °C heat pretreated sludge inoculated with Enterococcus sp. LG1), T (65 °C heat pretreated sludge), and TB + HB (Thermophilic Bacteria pretreated sludge inoculated with Enterococcus sp. LG1), respectively. The lag time of TB was only 3 h. The hydrogen concentration in the gas phase of TB + HB and T + HB was 66.2 and 67%, respectively, higher than that of TB and T. These r...
