The Experts below are selected from a list of 24234 Experts worldwide ranked by ideXlab platform
Edwin E Spencer - One of the best experts on this subject based on the ideXlab platform.
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partial Thickness articular surface rotator cuff tears an all inside repair technique
Clinical Orthopaedics and Related Research, 2010Co-Authors: Edwin E SpencerAbstract:Background Treatment of partial-Thickness articular surface rotator cuff tears varies from simple debridement with or without an acromioplasty to various repair techniques. These repair techniques have included in situ transtendinous methods, as well as completion of the tear and repairing the full-Thickness Defect. The transtendinous techniques can be associated with stiffness and completing the tear takes down normal intact tissue. Therefore, a technique was developed that repairs the articular-side partial- Thickness rotator cuff tears with an all-inside approach that does not violate the intact bursal tissue and does not complete the tear.
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partial Thickness articular surface rotator cuff tears an all inside repair technique
Techniques in Shoulder and Elbow Surgery, 2007Co-Authors: Edwin E SpencerAbstract:The treatment of partial-Thickness articular surface rotator cuff tears is controversial. Treatment has varied from simple debridement with or without an acromioplasty to various repair techniques. These repair techniques have included transtendinous methods and completion of the tear and repairing the full Thickness Defect. The transtendinous techniques can be associated with stiffness, and completing the tear takes down normal intact tissue. Therefore, a technique was developed that repairs the articular side partial-Thickness rotator cuff tears with an all-inside approach that does not violate the intact bursal tissue and does not complete the tear.
PP Parnigotto - One of the best experts on this subject based on the ideXlab platform.
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myoblast acellular skeletal muscle matrix constructs guarantee a long term repair of experimental full Thickness abdominal wall Defects
Tissue Engineering, 2006Co-Authors: Paolo De Coppi, Silvia Bellini, Maria Teresa Conconi, Morena Sabatti, Enea Simonato, Piergiorgio Gamba, Gastone G Nussdorfer, PP ParnigottoAbstract:To obtain a valuable treatment of congenital muscle Defect, cell-matrix constructs composed of satellite cell–derived myoblasts (XY karyotype) seeded on muscle acellular matrices were used to repair a previously created full-Thickness Defect of abdominal wall of 18 1-month-old female Lewis rats. Acellular abdominal matrices, obtained by a detergent-enzymatic method, were positive for both basic fibroblast growth factor and transforming growth factor-β, and were able to support in vitro cell adhesion. All animals survived the surgery, without signs of infection or implant rejection, and were humanely killed at 1, 3, or 9 months after surgery. The implants appeared well preserved, were integrated in the host tissue, and maintained their original dimension and Thickness until 9 months. Vesicular acetylcholine transporter was expressed on the surface of muscle fibers from 1 month postsurgery. Finally, implanted male myoblasts were present inside the patches until 9 months, as demonstrated by the expression of...
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Myoblast-acellular skeletal muscle matrix constructs guarantee a long-term repair of experimental full-Thickness abdominal wall Defects
TISSUE ENG, 2006Co-Authors: PP ParnigottoAbstract:To obtain a valuable treatment of congenital muscle Defect, cell-matrix constructs composed of satellite cell-derived myoblasts (XY karyotype) seeded on muscle acellular matrices were used to repair a previously created full-Thickness Defect of abdominal wall of 18 1-month-old female Lewis rats. Acellular abdominal matrices, obtained by a detergent-enzymatic method, were positive for both basic fibroblast growth factor and transforming growth factor-beta, and were able to support in vitro cell adhesion. All animals survived the surgery, without signs of infection or implant rejection, and were humanely killed at 1, 3, or 9 months after surgery. The implants appeared well preserved, were integrated in the host tissue, and maintained their original dimension and Thickness until 9 months. Vesicular acetylcholine transporter was expressed on the surface of muscle fibers from 1 month postsurgery. Finally, implanted male myoblasts were present inside the patches until 9 months, as demonstrated by the expression of SrY mRNA and by the presence of Y chromosome probe signal. These results allow us to conclude that cell-matrix constructs could represent a promising approach to the repair of muscle Defects, because they are repopulated in vivo by skeletal muscle cells and nervous elements and maintain their structural integrity over the long term.
Tae Soo Bae - One of the best experts on this subject based on the ideXlab platform.
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Regeneration of a full-Thickness Defect of rotator cuff tendon with freshly thawed umbilical cord-derived mesenchymal stem cells in a rat model
Stem Cell Research & Therapy, 2020Co-Authors: Jihye Yea, Inja Kim, Gayoung Sym, Jinkyung Park, Tae Soo BaeAbstract:Background It is difficult to immediately use mesenchymal stem cells (MSCs) for the patient with rotator cuff disease because isolation and culture time are required. Thus, the MSCs would be prepared in advanced in cryopreserved condition for an “off-the-shelf” usage in clinic. This study investigated the efficacy of freshly thawed MSCs on the regeneration of a full-Thickness tendon Defect (FTD) of rotator cuff tendon in a rat model. Methods We evaluated morphology, viability, and proliferation of cultured umbilical cord-derived MSCs (C-UC MSCs) and freshly thawed umbilical cord-derived MSCs (T-UC MSCs) at passage 10 in vitro. In animal experiments, we created a FTD in the supraspinatus of rats and injected the injured tendon with saline, cryopreserved agent (CPA; control), C-UC MSCs, and T-UC MSCs, respectively. Two and 4 weeks later, macroscopic, histological, biomechanical, and cell trafficking were evaluated. T test and ANOVA were used with SPSS. Differences with p
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regeneration of a full Thickness Defect in rotator cuff tendon with umbilical cord derived mesenchymal stem cells in a rat model
bioRxiv, 2020Co-Authors: Jihye Yea, Inja Kim, Gayoung Sym, Jinkyung Park, Ahyoung Lee, Byeong Chan Cho, Tae Soo Bae, Byoung Jae KimAbstract:Although rotator cuff disease is a common cause of shoulder pain, there is still no treatment method that could halt or reveres its development and progression. The purpose of this study was to investigate the efficacy of umbilical cord-derived mesenchymal stem cells (UC MSCs) on the regeneration of a full-Thickness rotator cuff Defect (FTD) in a rat model. We injected either UC MSCs or saline to the FTD and investigated macroscopic, histological and biomechanical results and cell trafficking. Treatment with UC MSCs improved macroscopic appearance in terms of tendon Thickness at two weeks, and inflammation, Defect size, swelling/redness and connection surrounding tissue and slidability at four weeks compared to the saline group. Histologically, UC MSCs induced the tendon matrix formation recovering collagen organization, nuclear aspect ratio and orientation angle of fibroblast as well as suppressing cartilage-related glycosaminoglycan compared to saline group at four weeks. The UC MSCs group also improved ultimate failure load by 25.0% and 19.0% and ultimate stress by 27.3% and 26.8% at two and four weeks compared to saline group. UC MSCs labeled with PKH26 exhibited 5.3% survival at four weeks compared to three hours after injection. This study demonstrated that UC MSCs regenerated the FTD with tendon tissue similar properties to the normal tendon in terms of macroscopic, histological and biomechanical characteristics in a rat model.
Guang Yang - One of the best experts on this subject based on the ideXlab platform.
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biodegradable and electroactive regenerated bacterial cellulose mxene ti3c2tx composite hydrogel as wound dressing for accelerating skin wound healing under electrical stimulation
Advanced Healthcare Materials, 2020Co-Authors: Lin Mao, Yihua Gao, Li Wang, Weiwei Zhao, Haoyan Cheng, Lin Xia, Shangxian Xie, Zhijun Shi, Guang YangAbstract:Traditional wound dressings mainly participate in the passive healing processes and are rarely engaged in active wound healing by stimulating skin cell behaviors. Electrical stimulation (ES) has been known to regulate skin cell behaviors. Herein, a series of multifunctional hydrogels based on regenerated bacterial cellulose (rBC) and MXene (Ti3 C2 Tx ) are first developed that can electrically modulate cell behaviors for active skin wound healing under external ES. The composite hydrogel with 2 wt% MXene (rBC/MXene-2%) exhibits the highest electrical conductivity and the best biocompatibility. Meanwhile, the rBC/MXene-2% hydrogel presents desired mechanical properties, favorable flexibility, good biodegradability, and high water-uptake capacity. An in vivo study using a rat full-Thickness Defect model reveals that this rBC/MXene hydrogel exhibits a better therapeutic effect than the commercial Tegaderm film. More importantly, in vitro and in vivo data demonstrate that coupling with ES, the hydrogel can significantly enhance the proliferation activity of NIH3T3 cells and accelerate the wound healing process, as compared to non-ES controls. This study suggests that the biodegradable and electroactive rBC/MXene hydrogel is an appealing candidate as a wound dressing for skin wound healing, while also providing an effective synergistic therapeutic strategy for accelerating wound repair process through coupling ES with the hydrogel dressing.
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biodegradable and electroactive regenerated bacterial cellulose mxene ti 3 c 2 t x composite hydrogel as wound dressing for accelerating skin wound healing under electrical stimulation
Advanced Healthcare Materials, 2020Co-Authors: Lin Mao, Yihua Gao, Li Wang, Weiwei Zhao, Haoyan Cheng, Lin Xia, Shangxian Xie, Zhijun Shi, Guang YangAbstract:Traditional wound dressings mainly participate in the passive healing processes and are rarely engaged in active wound healing by stimulating skin cell behaviors. Electrical stimulation (ES) has been known to regulate skin cell behaviors. Herein, a series of multifunctional hydrogels based on regenerated bacterial cellulose (rBC) and MXene (Ti3 C2 Tx ) are first developed that can electrically modulate cell behaviors for active skin wound healing under external ES. The composite hydrogel with 2 wt% MXene (rBC/MXene-2%) exhibits the highest electrical conductivity and the best biocompatibility. Meanwhile, the rBC/MXene-2% hydrogel presents desired mechanical properties, favorable flexibility, good biodegradability, and high water-uptake capacity. An in vivo study using a rat full-Thickness Defect model reveals that this rBC/MXene hydrogel exhibits a better therapeutic effect than the commercial Tegaderm film. More importantly, in vitro and in vivo data demonstrate that coupling with ES, the hydrogel can significantly enhance the proliferation activity of NIH3T3 cells and accelerate the wound healing process, as compared to non-ES controls. This study suggests that the biodegradable and electroactive rBC/MXene hydrogel is an appealing candidate as a wound dressing for skin wound healing, while also providing an effective synergistic therapeutic strategy for accelerating wound repair process through coupling ES with the hydrogel dressing.
Jihye Yea - One of the best experts on this subject based on the ideXlab platform.
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Regeneration of a full-Thickness Defect of rotator cuff tendon with freshly thawed umbilical cord-derived mesenchymal stem cells in a rat model
Stem Cell Research & Therapy, 2020Co-Authors: Jihye Yea, Inja Kim, Gayoung Sym, Jinkyung Park, Tae Soo BaeAbstract:Background It is difficult to immediately use mesenchymal stem cells (MSCs) for the patient with rotator cuff disease because isolation and culture time are required. Thus, the MSCs would be prepared in advanced in cryopreserved condition for an “off-the-shelf” usage in clinic. This study investigated the efficacy of freshly thawed MSCs on the regeneration of a full-Thickness tendon Defect (FTD) of rotator cuff tendon in a rat model. Methods We evaluated morphology, viability, and proliferation of cultured umbilical cord-derived MSCs (C-UC MSCs) and freshly thawed umbilical cord-derived MSCs (T-UC MSCs) at passage 10 in vitro. In animal experiments, we created a FTD in the supraspinatus of rats and injected the injured tendon with saline, cryopreserved agent (CPA; control), C-UC MSCs, and T-UC MSCs, respectively. Two and 4 weeks later, macroscopic, histological, biomechanical, and cell trafficking were evaluated. T test and ANOVA were used with SPSS. Differences with p
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regeneration of a full Thickness Defect in rotator cuff tendon with umbilical cord derived mesenchymal stem cells in a rat model
bioRxiv, 2020Co-Authors: Jihye Yea, Inja Kim, Gayoung Sym, Jinkyung Park, Ahyoung Lee, Byeong Chan Cho, Tae Soo Bae, Byoung Jae KimAbstract:Although rotator cuff disease is a common cause of shoulder pain, there is still no treatment method that could halt or reveres its development and progression. The purpose of this study was to investigate the efficacy of umbilical cord-derived mesenchymal stem cells (UC MSCs) on the regeneration of a full-Thickness rotator cuff Defect (FTD) in a rat model. We injected either UC MSCs or saline to the FTD and investigated macroscopic, histological and biomechanical results and cell trafficking. Treatment with UC MSCs improved macroscopic appearance in terms of tendon Thickness at two weeks, and inflammation, Defect size, swelling/redness and connection surrounding tissue and slidability at four weeks compared to the saline group. Histologically, UC MSCs induced the tendon matrix formation recovering collagen organization, nuclear aspect ratio and orientation angle of fibroblast as well as suppressing cartilage-related glycosaminoglycan compared to saline group at four weeks. The UC MSCs group also improved ultimate failure load by 25.0% and 19.0% and ultimate stress by 27.3% and 26.8% at two and four weeks compared to saline group. UC MSCs labeled with PKH26 exhibited 5.3% survival at four weeks compared to three hours after injection. This study demonstrated that UC MSCs regenerated the FTD with tendon tissue similar properties to the normal tendon in terms of macroscopic, histological and biomechanical characteristics in a rat model.
