Thidiazuron

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Miklos Faust - One of the best experts on this subject based on the ideXlab platform.

  • Ascorbic Acid Oxidase Activity in Apple Buds: Relation to Thidiazuron- induced Lateral Budbreak
    HortScience, 1992
    Co-Authors: Shiow Y. Wang, Miklos Faust
    Abstract:

    The activity of ascorbic acid oxidase (AAO) was studied in apple (Malus domestica Borkh.) buds during dormancy and Thidiazuron-induced budbreak. In dor- mant buds, activity of AAO was low compared with buds that were treated with Thidiazuron and had resumed growth. An increase in AAO activity began at the time of metabolic transition from dormancy to budbreak. The highest level of activity was reached 10 days after Thidiazuron induction during the expansion growth phase. In vitro AAO activity of apple bud extract was increased by addition of Cu (CuSO 4) and inhibited by Cu-chelating agents, diethyldithiocarbamate (DDC), sodium azide (NaN 3), and 8-hydroxyquinoiine (8-OH-Q). In vivo treatment of apple buds with Cu-chelating agents inhibited AAO activity and bud growth but not budbreak. Chemical name used: N- phenyl -N' -1,2,3-thidiazol-5-ylurea (Thidiazuron).

  • changes in ascorbate glutathione and related enzyme activities during Thidiazuron induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

  • Changes in Metabolic Enzyme Activities during Thidiazuron-induced Lateral Budbreak of Apple
    HortScience, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The activity of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphate gluconate dehydrogenase (5PGDH), isocitrate dehydrogenase (ICDH), pyruvate kinase (PK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were studied in apple (Malus domestica Borkh.) buds during dormancy and Thidiazuron-induced budbreak. When buds were dormant, the activity of the glycolytic enzymes GAPDH and PK and the tricarboxylic acid (TCA) cycle enzyme ICDH was low compared to that in non-dormant buds. The activity of these enzymes increased during budbreak, peaked when buds were in the green tip stage just before the start of rapid expansion (at 8 days after Thidiazuron treatment), and declined thereafter (...)

  • Changes in superoxide dismutase activity during Thidiazuron-induced lateral budbreak of apple
    HortScience, 1991
    Co-Authors: S. Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The effects of Thidiazuron on changes in superoxide dismutase (SOD) activity in apple (Malus domestica Borkh cv. York Imperial) buds during dormancy break were determined. Dormant buds bad low SOD activity. Increased SOD activity was correlated with budbreak induced by Thidiazuron. Buds contained three distinct forms of SOD: Cu, Zn-SOD; Mn-SOD; and Fe-SOD. Most of the SOD activity in apple buds was attributed to Cu and Zn-SOD, followed by Mn-SOD

  • Changes in ascorbate, glutathione, and related enzyme activities during Thidiazuron‐induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

Shiow Y. Wang - One of the best experts on this subject based on the ideXlab platform.

  • Ascorbic Acid Oxidase Activity in Apple Buds: Relation to Thidiazuron- induced Lateral Budbreak
    HortScience, 1992
    Co-Authors: Shiow Y. Wang, Miklos Faust
    Abstract:

    The activity of ascorbic acid oxidase (AAO) was studied in apple (Malus domestica Borkh.) buds during dormancy and Thidiazuron-induced budbreak. In dor- mant buds, activity of AAO was low compared with buds that were treated with Thidiazuron and had resumed growth. An increase in AAO activity began at the time of metabolic transition from dormancy to budbreak. The highest level of activity was reached 10 days after Thidiazuron induction during the expansion growth phase. In vitro AAO activity of apple bud extract was increased by addition of Cu (CuSO 4) and inhibited by Cu-chelating agents, diethyldithiocarbamate (DDC), sodium azide (NaN 3), and 8-hydroxyquinoiine (8-OH-Q). In vivo treatment of apple buds with Cu-chelating agents inhibited AAO activity and bud growth but not budbreak. Chemical name used: N- phenyl -N' -1,2,3-thidiazol-5-ylurea (Thidiazuron).

  • changes in ascorbate glutathione and related enzyme activities during Thidiazuron induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

  • Changes in Metabolic Enzyme Activities during Thidiazuron-induced Lateral Budbreak of Apple
    HortScience, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The activity of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphate gluconate dehydrogenase (5PGDH), isocitrate dehydrogenase (ICDH), pyruvate kinase (PK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were studied in apple (Malus domestica Borkh.) buds during dormancy and Thidiazuron-induced budbreak. When buds were dormant, the activity of the glycolytic enzymes GAPDH and PK and the tricarboxylic acid (TCA) cycle enzyme ICDH was low compared to that in non-dormant buds. The activity of these enzymes increased during budbreak, peaked when buds were in the green tip stage just before the start of rapid expansion (at 8 days after Thidiazuron treatment), and declined thereafter (...)

  • Changes in ascorbate, glutathione, and related enzyme activities during Thidiazuron‐induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

Leela George - One of the best experts on this subject based on the ideXlab platform.

  • Thidiazuron induced shoot regeneration in pigeonpea cajanus cajan l
    Plant Cell Tissue and Organ Culture, 1998
    Co-Authors: Susan Eapen, Suchita Tivarekar, Leela George
    Abstract:

    Thidiazuron either alone or in combination with IAA induced high frequency shoot regeneration from primary leaf segments of three pigeonpea cultivars. Transfer of the cultures to medium with reduced concentration of Thidiazuron resulted in further development of the shoots. The regenerated shoots were subsequently transferred to medium supplemented with BA, IAA and gibberellic acid where 5-10% of the shoots elongated further. Rooting of shoots could be obtained on half strength MS medium supplemented with NAA. Histological studies confirmed the mode of regeneration as shoot organogenesis.

Hans-jörg Jacobsen - One of the best experts on this subject based on the ideXlab platform.

  • Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus.
    Plant Cell Reports, 1995
    Co-Authors: Petra Böhmer, Beate Meyer, Hans-jörg Jacobsen
    Abstract:

    Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1–50µM) of Thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these Thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.

Hong J. Jiao - One of the best experts on this subject based on the ideXlab platform.

  • changes in ascorbate glutathione and related enzyme activities during Thidiazuron induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

  • Changes in Metabolic Enzyme Activities during Thidiazuron-induced Lateral Budbreak of Apple
    HortScience, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The activity of glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphate gluconate dehydrogenase (5PGDH), isocitrate dehydrogenase (ICDH), pyruvate kinase (PK), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were studied in apple (Malus domestica Borkh.) buds during dormancy and Thidiazuron-induced budbreak. When buds were dormant, the activity of the glycolytic enzymes GAPDH and PK and the tricarboxylic acid (TCA) cycle enzyme ICDH was low compared to that in non-dormant buds. The activity of these enzymes increased during budbreak, peaked when buds were in the green tip stage just before the start of rapid expansion (at 8 days after Thidiazuron treatment), and declined thereafter (...)

  • Changes in superoxide dismutase activity during Thidiazuron-induced lateral budbreak of apple
    HortScience, 1991
    Co-Authors: S. Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The effects of Thidiazuron on changes in superoxide dismutase (SOD) activity in apple (Malus domestica Borkh cv. York Imperial) buds during dormancy break were determined. Dormant buds bad low SOD activity. Increased SOD activity was correlated with budbreak induced by Thidiazuron. Buds contained three distinct forms of SOD: Cu, Zn-SOD; Mn-SOD; and Fe-SOD. Most of the SOD activity in apple buds was attributed to Cu and Zn-SOD, followed by Mn-SOD

  • Changes in ascorbate, glutathione, and related enzyme activities during Thidiazuron‐induced bud break of apple
    Physiologia Plantarum, 1991
    Co-Authors: Shiow Y. Wang, Hong J. Jiao, Miklos Faust
    Abstract:

    The changes of ascorbic acid, dehydroascorbic acid, and glutathione content and related enzyme activities were studied in apple buds during dormancy and Thidiazuron-induced bud break. An increase in ascorbic acid, reduced form of glutathione (GSH), total glutathione, total non-protein thiol (NPSH) and non-glutathione thiol (RSH) occurred as a result of induction by Thidiazuron during bud break, whereas dehydroascorbic acid and oxidized glutathione (GSSG) decreased during the same period. Thidiazuron also enhanced the ratio of GSH/GSSG, and activities of ascorbate free radical reductase (AFR; EC 1.6.5.4), ascorbate peroxidase (EC 1.11.1.11). dehydroascorbate reductase (DHAR; EC 1.8.5.1) and glutathione reductase (GR; EC 1.6.4.2). The ascorbic acid content and the activities of AFR, ascorbate peroxidase, and DHAR peaked when buds were in the side green or green tip stage just prior to the start of rapid expansion, and declined thereafter. The GSH, NPSH, RSH, ratio of GSH/GSSG, and activities of GR increased steadily during bud development.

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