The Experts below are selected from a list of 1797 Experts worldwide ranked by ideXlab platform
Nicoletta Sacchi - One of the best experts on this subject based on the ideXlab platform.
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the single step method of rna isolation by acid guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium Thiocyanate, Sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
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The single-step method of RNA isolation by acid guanidinium Thiocyanate–phenol–chloroform extraction: twenty-something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium Thiocyanate, Sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
Piotr Chomczynski - One of the best experts on this subject based on the ideXlab platform.
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the single step method of rna isolation by acid guanidinium Thiocyanate phenol chloroform extraction twenty something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium Thiocyanate, Sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
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The single-step method of RNA isolation by acid guanidinium Thiocyanate–phenol–chloroform extraction: twenty-something years on
Nature Protocols, 2006Co-Authors: Piotr Chomczynski, Nicoletta SacchiAbstract:Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is separated from DNA after extraction with an acidic solution containing guanidinium Thiocyanate, Sodium acetate, phenol and chloroform, followed by centrifugation. Under acidic conditions, total RNA remains in the upper aqueous phase, while most of DNA and proteins remain either in the interphase or in the lower organic phase. Total RNA is then recovered by precipitation with isopropanol and can be used for several applications. The original protocol, enabling the isolation of RNA from cells and tissues in less than 4 hours, greatly advanced the analysis of gene expression in plant and animal models as well as in pathological samples, as demonstrated by the overwhelming number of citations the paper gained over 20 years.
Lukas J Goossen - One of the best experts on this subject based on the ideXlab platform.
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sandmeyer trifluoromethylthiolation of arenediazonium salts with Sodium Thiocyanate and ruppert prakash reagent
Chemical Science, 2014Co-Authors: Grégory Danoun, Matthias F Gruenberg, Bilguun Bayarmagnai, Lukas J GoossenAbstract:In the presence of copper Thiocyanate, Sodium Thiocyanate and the inexpensive, easy-to-use trifluoromethylating reagent Me3Si–CF3, diazonium salts are smoothly converted into the corresponding aryl trifluoromethyl thioethers. Combined with diazotisation, this convenient and inexpensive method allows the straightforward synthesis of aryl or heteroaryl trifluoromethyl thioethers from the corresponding anilines.
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Sandmeyer trifluoromethylthiolation of arenediazonium salts with Sodium Thiocyanate and Ruppert–Prakash reagent
Chemical Science, 2014Co-Authors: Grégory Danoun, Matthias F Gruenberg, Bilguun Bayarmagnai, Lukas J GoossenAbstract:In the presence of copper Thiocyanate, Sodium Thiocyanate and the inexpensive, easy-to-use trifluoromethylating reagent Me3Si–CF3, diazonium salts are smoothly converted into the corresponding aryl trifluoromethyl thioethers. Combined with diazotisation, this convenient and inexpensive method allows the straightforward synthesis of aryl or heteroaryl trifluoromethyl thioethers from the corresponding anilines.
Grégory Danoun - One of the best experts on this subject based on the ideXlab platform.
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sandmeyer trifluoromethylthiolation of arenediazonium salts with Sodium Thiocyanate and ruppert prakash reagent
Chemical Science, 2014Co-Authors: Grégory Danoun, Matthias F Gruenberg, Bilguun Bayarmagnai, Lukas J GoossenAbstract:In the presence of copper Thiocyanate, Sodium Thiocyanate and the inexpensive, easy-to-use trifluoromethylating reagent Me3Si–CF3, diazonium salts are smoothly converted into the corresponding aryl trifluoromethyl thioethers. Combined with diazotisation, this convenient and inexpensive method allows the straightforward synthesis of aryl or heteroaryl trifluoromethyl thioethers from the corresponding anilines.
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Sandmeyer trifluoromethylthiolation of arenediazonium salts with Sodium Thiocyanate and Ruppert–Prakash reagent
Chemical Science, 2014Co-Authors: Grégory Danoun, Matthias F Gruenberg, Bilguun Bayarmagnai, Lukas J GoossenAbstract:In the presence of copper Thiocyanate, Sodium Thiocyanate and the inexpensive, easy-to-use trifluoromethylating reagent Me3Si–CF3, diazonium salts are smoothly converted into the corresponding aryl trifluoromethyl thioethers. Combined with diazotisation, this convenient and inexpensive method allows the straightforward synthesis of aryl or heteroaryl trifluoromethyl thioethers from the corresponding anilines.
Sekh Mahiuddin - One of the best experts on this subject based on the ideXlab platform.
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Mixed anion effect in Sodium Thiocyanate + Sodium thiosulfate + water systems
Canadian Journal of Chemistry, 1998Co-Authors: G Kalita, N N Dass, Sekh MahiuddinAbstract:Densities and viscosities of R(xNaSCN + (1 - x)Na2S2O3 )+( 1 - R)H2O systems with R = 0.05, 0.10, 0.14, and 0.18 were measured as functions of mole fraction, x (= 0.0-1.0), and temperature (293.15 ≤ T/K ≤ 323.15). A significant mixed anion effect has been observed within the temperature range of the study. The normalized viscosity isotherms were found to detect the mixed anion effect ~1.2 to 3.7 times more than the simple viscosity isotherms. The progressive replacement of SO 23 2 - ions by SCN - ions causes the systems to be less structured and SO 23 2 - ions polarized Na + ions more towards themselves than towards the SCN - ions. Both these effects govern the variation of the viscosity with mole fraction, x. The mixed anion effect was found to vanish at around R = 0.0106.
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Viscosity and Molar Volume of Potassium Thiocyanate + Sodium Thiocyanate + Acetamide Melt Systems
Journal of Chemical & Engineering Data, 1998Co-Authors: G Kalita, Nashiour Rohman, Sekh MahiuddinAbstract:Density and viscosity of 0.25[xKSCN + (1 − x)NaSCN] + 0.75CH3CONH2 melt systems were measured as functions of temperature (293.15 ≤ T/K ≤ 343.15) and mole fraction, x = 0.0 to 1.0. The temperature ...