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Renyuan Song - One of the best experts on this subject based on the ideXlab platform.
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facile preparation of superparamagnetic surface imprinted microspheres using amino acid as template for specific capture of Thymopentin
Applied Surface Science, 2015Co-Authors: Longxia Guo, Ping Guan, Dan Wang, Dongmen Song, Xumian Gao, Renyuan SongAbstract:Abstract Novel superparamagnetic surface-imprinted microspheres (SIMs) with molecularly imprinted shell layer were controllably synthesized via fragment imprinting and surface imprinting technique. The SIMs-Arg and SIMs-Lys microspheres were prepared by using L-arginine (L-Arg) and L-lysine (L-Lys) as pseudo-template molecule for specific rebinding to Thymopentin (TP5), respectively. The characterization results revealed that both SIMs-Arg and SIMs-Lys were successfully prepared and possessed a high magnetic sensitivity. The rebinding-isotherm analyses of SIMs-Arg and SIMs-Lys showed that the Langmuir isotherm model was well fitted to the equilibrium data, indicating that only one kind of rebinding site was present in SIMs-Arg and SIMs-Lys. Besides, the kinetic properties of SIMs-Arg and SIMs-Lys both were well described by the pseudo-second-order kinetics model, which indicated that a chemical process may be the rate-limiting step in the rebinding process. Moreover, the magnetic imprinted microspheres were found to have a higher specificity for TP5 than that for immunostimulating peptide human (IPH). What is more, SIMs-Arg and SIMs-Lys were successfully applied for TP5 determination in urine. According to the maximum adsorption capacity, the imprinting factor and real sample experiment, it was noted that SIMs-Arg had better specific adsorption property for TP5 than SIMs-Lys.
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water compatible surface imprinted microspheres for high adsorption and selective recognition of peptide drug from aqueous media
Journal of Materials Chemistry B, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Chaoli WangAbstract:Novel water-compatible ionic liquid-functionalized microspheres with molecularly imprinted shell layer were controllably synthesized via precipitation polymerization and surface imprinting technique. Here, a room-temperature ionic liquid was synthesized to prepare these surface-imprinted microspheres with excellent water solubility and multiple binding sites with template molecules. The peptide drug Thymopentin (TP5) was chosen as a template molecule, which is known as an immunomodulating agent. The as-prepared microspheres were fully characterized. Results reveal that ionic liquid incorporation significantly improves the adsorption of TP5. Moreover, the adsorption property and recognition capability towards TP5 are closely related to the synergetic effect of electrostatic interaction and hydrogen bonding. Through employing the synergetic effect of directional and non-directional interactions, the surface-imprinted microspheres exhibit high adsorption capacity, good selective recognition, and rapid binding ability for TP5. The surface-imprinted microspheres demonstrate potential usage for TP5 enrichment from other biomolecules, and the proposed method was successfully applied for TP5 determination in Thymopentin injection and urine.
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separation and purification of Thymopentin with molecular imprinting membrane by solid phase extraction disks
Journal of Pharmaceutical and Biomedical Analysis, 2015Co-Authors: Chaoli Wang, Liwei Qian, Ping Guan, Renyuan SongAbstract:The synthesis and performance of molecularly imprinted membranes (MIMs) as a solid phase extraction packing materials for the separation and purification of Thymopentin from crude samples was described. In order to increase structural selectivity and imprinting efficiency, surface-initiated ATRP and ionic liquid (1-vinyl-3-ethyl acetate imidazolium chloride) were used to prepare molecularly imprinting membranes. The results demonstrated that solid phase extraction disks stuffed by MIMs with ionic liquids as functional monomer demonstrated high isolation and purification of performance to the Thymopentin. The molecular recognition of Thymopentin was analyzed by using molecular modeling software.
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synthesis of water compatible surface imprinted composite microspheres with core shell structure for selective recognition of Thymopentin from aqueous solution
Journal of Materials Science, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Yimei TangAbstract:A novel water-compatible surface-imprinted core–shell microsphere, which had multiple non–covalent interactions with template molecule, was successfully prepared by the surface grafting polymerization method in acetonitrile–water systems with Thymopentin as template through ionic liquid-functionalized polyethyleneglycolmethacrylate-co-vinylimidazole microsphere as the matrix. The average diameter of matrix was 1 μm ± 20 nm and the thickness of imprinted layer was about 50 nm. The results of static adsorption experiments indicated that ionic liquid-functionalized molecularly imprinted microspheres showed the good adsorption capacity and specific recognition for template peptide. The binding-isotherm analysis showed that Langmuir isotherm models gave a good fit in the range of concentrations, suggesting that there was only one kind of binding site in imprinted layer. Measurements of the binding kinetics revealed that surface-imprinted composite microspheres reached peptide-adsorption equilibrium in 60 min and the maximum adsorption capacity for TP5 was 38.4 mg g−1. The effects of pH, salt concentration, and temperature on the adsorption capacities were investigated. The microspheres were found to have a high specificity for TP5 with little affinity for BSA and Hb. Finally, the core–shell microspheres can be reused with only 15.6 % decrease in TP5 adsorption capacity after six times.
Ping Guan - One of the best experts on this subject based on the ideXlab platform.
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facile preparation of superparamagnetic surface imprinted microspheres using amino acid as template for specific capture of Thymopentin
Applied Surface Science, 2015Co-Authors: Longxia Guo, Ping Guan, Dan Wang, Dongmen Song, Xumian Gao, Renyuan SongAbstract:Abstract Novel superparamagnetic surface-imprinted microspheres (SIMs) with molecularly imprinted shell layer were controllably synthesized via fragment imprinting and surface imprinting technique. The SIMs-Arg and SIMs-Lys microspheres were prepared by using L-arginine (L-Arg) and L-lysine (L-Lys) as pseudo-template molecule for specific rebinding to Thymopentin (TP5), respectively. The characterization results revealed that both SIMs-Arg and SIMs-Lys were successfully prepared and possessed a high magnetic sensitivity. The rebinding-isotherm analyses of SIMs-Arg and SIMs-Lys showed that the Langmuir isotherm model was well fitted to the equilibrium data, indicating that only one kind of rebinding site was present in SIMs-Arg and SIMs-Lys. Besides, the kinetic properties of SIMs-Arg and SIMs-Lys both were well described by the pseudo-second-order kinetics model, which indicated that a chemical process may be the rate-limiting step in the rebinding process. Moreover, the magnetic imprinted microspheres were found to have a higher specificity for TP5 than that for immunostimulating peptide human (IPH). What is more, SIMs-Arg and SIMs-Lys were successfully applied for TP5 determination in urine. According to the maximum adsorption capacity, the imprinting factor and real sample experiment, it was noted that SIMs-Arg had better specific adsorption property for TP5 than SIMs-Lys.
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water compatible surface imprinted microspheres for high adsorption and selective recognition of peptide drug from aqueous media
Journal of Materials Chemistry B, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Chaoli WangAbstract:Novel water-compatible ionic liquid-functionalized microspheres with molecularly imprinted shell layer were controllably synthesized via precipitation polymerization and surface imprinting technique. Here, a room-temperature ionic liquid was synthesized to prepare these surface-imprinted microspheres with excellent water solubility and multiple binding sites with template molecules. The peptide drug Thymopentin (TP5) was chosen as a template molecule, which is known as an immunomodulating agent. The as-prepared microspheres were fully characterized. Results reveal that ionic liquid incorporation significantly improves the adsorption of TP5. Moreover, the adsorption property and recognition capability towards TP5 are closely related to the synergetic effect of electrostatic interaction and hydrogen bonding. Through employing the synergetic effect of directional and non-directional interactions, the surface-imprinted microspheres exhibit high adsorption capacity, good selective recognition, and rapid binding ability for TP5. The surface-imprinted microspheres demonstrate potential usage for TP5 enrichment from other biomolecules, and the proposed method was successfully applied for TP5 determination in Thymopentin injection and urine.
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separation and purification of Thymopentin with molecular imprinting membrane by solid phase extraction disks
Journal of Pharmaceutical and Biomedical Analysis, 2015Co-Authors: Chaoli Wang, Liwei Qian, Ping Guan, Renyuan SongAbstract:The synthesis and performance of molecularly imprinted membranes (MIMs) as a solid phase extraction packing materials for the separation and purification of Thymopentin from crude samples was described. In order to increase structural selectivity and imprinting efficiency, surface-initiated ATRP and ionic liquid (1-vinyl-3-ethyl acetate imidazolium chloride) were used to prepare molecularly imprinting membranes. The results demonstrated that solid phase extraction disks stuffed by MIMs with ionic liquids as functional monomer demonstrated high isolation and purification of performance to the Thymopentin. The molecular recognition of Thymopentin was analyzed by using molecular modeling software.
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synthesis of water compatible surface imprinted composite microspheres with core shell structure for selective recognition of Thymopentin from aqueous solution
Journal of Materials Science, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Yimei TangAbstract:A novel water-compatible surface-imprinted core–shell microsphere, which had multiple non–covalent interactions with template molecule, was successfully prepared by the surface grafting polymerization method in acetonitrile–water systems with Thymopentin as template through ionic liquid-functionalized polyethyleneglycolmethacrylate-co-vinylimidazole microsphere as the matrix. The average diameter of matrix was 1 μm ± 20 nm and the thickness of imprinted layer was about 50 nm. The results of static adsorption experiments indicated that ionic liquid-functionalized molecularly imprinted microspheres showed the good adsorption capacity and specific recognition for template peptide. The binding-isotherm analysis showed that Langmuir isotherm models gave a good fit in the range of concentrations, suggesting that there was only one kind of binding site in imprinted layer. Measurements of the binding kinetics revealed that surface-imprinted composite microspheres reached peptide-adsorption equilibrium in 60 min and the maximum adsorption capacity for TP5 was 38.4 mg g−1. The effects of pH, salt concentration, and temperature on the adsorption capacities were investigated. The microspheres were found to have a high specificity for TP5 with little affinity for BSA and Hb. Finally, the core–shell microspheres can be reused with only 15.6 % decrease in TP5 adsorption capacity after six times.
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Thymopentin Magnetic Molecularly Imprinted Polymers with Room Temperature Ionic Liquids as a Functional Monomer by Surface-Initiated ATRP
International Journal of Polymer Analysis and Characterization, 2014Co-Authors: Chaoli Wang, Liwei Qian, Danfeng Wu, Xiaoling Hu, Ping Guan, Ji LiAbstract:Ionic liquids are also called “designer solvents.” In this article, we calculated the interaction energy of four ionic liquids with a template by molecular dynamics simulation. A simple approach was used to prepare biomacromolecule molecularly imprinted polymers for adsorbent and separation Thymopentin (Tp5). In order to overcome intrinsic and increase structural selectivity, surface-initiated ATRP and ionic liquids (ILs) as functional monomer were used to prepare Fe3O4 molecularly imprinted polymers. Selective adsorption was applied to investigate the interactions between the polymers and lysine, phenylalanine, glutathione (GSH), and hemoglobin. The results demonstrated that Fe3O4 MIPs with 1-vinyl-3-ethyl acetate imidazolium chloride as functional monomer demonstrated high isolation and recognition of performance to the Tp5.
Fengshan Wang - One of the best experts on this subject based on the ideXlab platform.
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the in vivo immunomodulatory and synergistic anti tumor activity of thymosin α1 Thymopentin fusion peptide and its binding to tlr2
Cancer Letters, 2013Co-Authors: Yanna Cheng, Qian Zhang, Xinke Zhang, Lei Zheng, Zhen Han, Yuliang Xiao, Fengshan WangAbstract:In the present study, the immunomodulatory and synergistic anti-tumor activity of thymosin α1-Thymopentin fusion peptide (Tα1-TP5) was investigated in vivo. In addition, the potential receptor of Tα1-TP5 was investigated by surface plasmon resonance (SPR) binding studies. It was found that Tα1-TP5 (305 μg/kg) alleviated immunosuppression induced by hydrocortisone (HC). Tα1-TP5 (305 μg/kg) combined with cyclophosphamide (CY) had a better tumor growth inhibitory effect than CY alone. Furthermore, Tα1-TP5 had a higher affinity (KD=6.84 μmol/L) to toll-like receptor 2 (TLR2) than Tα1 (K(D)=35.4 μmol/L), but its affinity was not significantly different from that of TP5. The results of our present work indicate that Tα1-TP5 can possibly be developed as a new immunomodulatory agent.
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intein mediated expression purification and characterization of thymosin α1 Thymopentin fusion peptide in escherichia coli
Protein Expression and Purification, 2012Co-Authors: Lei Zheng, Fengshan WangAbstract:Thymosin α1-Thymopentin (Tα1-TP5) fusion peptide has been proved to be an immune regulator based on its higher immunoregulatory activity than Tα1 and TP5. To obtain Tα1-TP5 more effectively and economically, Tα1-TP5 was genetically fused to a self-cleaving intein-chitin binding domain tag for purification via chitin beads in Escherichia coli. After affinity purification, the target peptide was released from the chitin beads via self-cleaving intein ((INTervening protEIN) induced by dithiothreitol. Further, Tα1-TP5 was purified by Superdex 30 and identified by Tricine-SDS-PAGE and electrospray ionization-mass spectrometry. Finally, about 7.6 mg Tα1-TP5 purified from the soluble fraction and inclusion bodies was obtained from 1 L culture media. The purity was 95% after a series of chromatographic purification steps. In vitro, the purified Tα1-TP5 could stimulate the proliferation of mouse splenic lymphocytes. Overall, this work demonstrated that Tα1-TP5 was purified with low cost and high efficiency, greatly expanding its potential use as an immune regulator.
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expression of thymosin α1 Thymopentin fusion peptide in pichia pastoris and its characterization
Archives of Pharmacal Research, 2008Co-Authors: Demin Gao, Xulong Zhang, Jian Zhang, Jichao Cao, Fengshan WangAbstract:Thymopentin plays an important role in improving imbalanced immune systems of patients, however, it has a limited half-life in plasma. To get more stable and active Thymopentin analogs, a fusion thymosin α1-Thymopentin (Tα1-TP5) gene was synthesized and cloned into vector pGAPZαA. Tα1-TP5 fusion peptide was expressed in pichia pastoris and purified by metal chelating chromatography and gel filtration chromatography. The circular dichroism spectra (CD) indicated that the secondary structure of Tα1-TP5 fusion peptide is dominated by a-helix and random coil. In vitro analysis showed that the plasma half-life of Tα1-TP5 fusion peptide is 140 ± 14 min, which is longer than that of TP5 (5.6±0.7 min) and Tα1 (127±11 min). The in vitro activity assay presented that Tα1-TP5 fusion peptide has greater activity in promoting proliferation of Kunming mouse splenocytes, and in vivo experiment it showed better activity in promoting the phagocytosis of macrophages and secretion of IL-2 than both Tα1 and TP5. Our findings suggest that Tα1-TP5 fusion peptide might be a potential therapeutic agent.
Zhi-rong Zhang - One of the best experts on this subject based on the ideXlab platform.
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implantable sandwich phbhhx film for burst free controlled delivery of Thymopentin peptide
Acta Pharmaceutica Sinica B, 2018Co-Authors: Ke Peng, Zhi-rong Zhang, Guoxu Wei, Jinghui Jiang, Xun SunAbstract:Abstract Sustained release and non-parental formulations of peptides and protein drugs are highly desirable because of enhanced therapeutic effects as well as improved patient compliance. This is especially true for small peptides such as Thymopentin (TP5). To this end, implantable sandwich poly (hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) films were designed to prolong release time and to inhibit burst release phenomenon of TP5 by a simple volatilization method. In vitro release studies revealed that sandwich films had nearly no burst release. In vivo release time of sandwich films was prolonged to 42 days. Pharmacodynamic evaluation demonstrated that TP5 sandwich films significantly increased survival rates in a rat immunosuppressive model and normalized CD4+/CD8+ values. These results suggest that TP5 released from sandwich films can attenuate cyclophosphamide's immunosuppressive activity, and possibly achieve results comparable to daily TP5 injection therapy. Thus, sandwich PHBHHx films show excellent potential as a sustained, burst-free release system for small molecular weight, hydrophilic peptide drugs.
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Thymopentin nanoparticles engineered with high loading efficiency improved pharmacokinetic properties and enhanced immunostimulating effect using soybean phospholipid and phbhhx polymer
Molecular Pharmaceutics, 2014Co-Authors: Mengtian Zhang, Zhi-rong Zhang, Kawai Wan, Waqar Ahmed, David A Phoenix, Abdelbary Elhissi, Xun SunAbstract:Formulation of protein and peptide drugs with sustained release properties is crucial to enhance their therapeutic effect and minimize administration frequency. In this study, immunomodulating polymeric systems were designed by manufacturing PHBHHx nanoparticles (NPs) containing Thymopentin (TP5). The release profile of the drug was studied over a period of 7 days. The PHBHHx NPs containing TP5-phospholipid (PLC) complex (TP5-PLC) displayed a spherical shape with a mean size, zeta potential, and encapsulation efficiency of 238.9 nm, -32.0 mV, and 72.81%, respectively. The cytotoxicity results showed the PHBHHx NPs had a relatively low toxicity in vitro. TP5 entrapped in the NPs could hardly release in vitro, while the NPs had longer than 7 days release duration after a single subcutaneous injection in Wistar rats. The immunodepression rat model was built to evaluate the immunomodulating effects of TP5-PLC-NPs in vivo. The results of T-lymphocyte subsets (CD3(+), CD4(+), CD8(+), and CD4(+)/CD8(+) ratio) analysis and superoxide dismutase (SOD) values suggested that TP5-PLC-NPs had stronger immunoregulation effects than TP5 solution. In conclusion, an applicable approach to markedly enhancing the loading of a water-soluble peptide into a hydrophobic polymer matrix has been introduced. Thus, TP5-PLC-NPs are promising nanomedicine systems for sustained release effects of TP5.
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vesicular phospholipid gels using low concentrations of phospholipids for the sustained release of Thymopentin pharmacokinetics and pharmacodynamics
Die Pharmazie, 2013Co-Authors: Ying Zhong, Liyu Chen, Yu Zhang, Xun Sun, Tao Gong, Zhi-rong ZhangAbstract:Vesicular phospholipid gels (VPGs) with high concentrations of phospholipids are used as implantable depots for sustained release of drugs due to high viscosity. This study aimed to investigate VPGs with low concentrations of phospholipids for subcutaneous injection and sustained release in vivo. A small peptide, Thymopentin, was selected and incorporated into various VPG formulations. The VPG viscosity was greatly increased with higher concentrations of phospholipids (E80) and thus VPGs based on low lipid contents are more suitable for injection. Additionally, VPGs loaded with 5-hydroxy-fluorescein-thymopetin (5-FAM-TP5-VPGs) were developed and their pharmacokinetic profile was investigated in vivo. After subcutaneous injection, the release time of 5-FAM-TP5 was 216 h for 5-FAM-TP5-VPGs (containing 300 mg/g lipid), which was much longer than that of 5-FAM-TP5 solution. The therapeutic efficacy of TP5-VPGs (containing 300 mg/g lipid) after subcutaneous administration once a week was demonstrated to be comparable to that of TP5 solution injected subcutaneously once daily for 7 days. In conclusion, TP5-VPGs with low lipid content (300 mg/g) displayed sustained release properties in vivo that may serve as a sustained delivery system for subcutaneous injection.
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multivesicular liposomes for the sustained release of Thymopentin stability pharmacokinetics and pharmacodynamics
Die Pharmazie, 2012Co-Authors: Jiao Zuo, Xun Sun, Tao Gong, Yuan Huang, Qiang Peng, Zhi-rong ZhangAbstract:The objective of the present study was to investigate the storage stability of Thymopentin multivesicular liposomes (TP5-MVLs) prepared with different emulsifiers, and to study the pharmacokinetics and pharmacodynamics of the produced TP5-MVLs in vivo. The stability studies of TP5-MVLs indicated that MVLs particles prepared with mixed emulsifiers (Myrj52:solutolHS15 = 2:3) were stable at the storage temperature of 4 +/- 2 degrees C within 3 months. In addition, FITC-TP5-loaded MVLs was prepared for pharmacokinetic studies that after subcutaneous administration, the fluorescence signal lasted for about 5 days in plasma demonstrating that the rate of drug release from MVLs was very slow. The pharmacodynamic studies indicated that the therapeutic efficacy of TP5-MVLs after subcutaneous administration once every four days was the same as free TP5 solution after intravenous or subcutaneous administration once daily. In conclusion, MVLs, which possessed great storage stability, can be utilized to reduce the administration frequency of TP5, and therefore, served as a promising sustained release delivery system for polypeptide.
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inhalable microparticles as carriers for pulmonary delivery of Thymopentin loaded solid lipid nanoparticles
Pharmaceutical Research, 2010Co-Authors: Xun Sun, Tao Gong, Jiao Zuo, Jie Liu, Zhi-rong ZhangAbstract:Microparticles containing solid lipid nanoparticles (SLNs) are receiving increased attention as carriers for the lung delivery of the SLNs. Thus, we aim to prepare the hybrid microparticles and thoroughly evaluate their feasibility for the pulmonary drug delivery. The microparticles were prepared by co-spray-drying the Thymopentin (TP5)-loaded SLNs with bulking agents. Thereafter, we systematically estimated the potential of the microparticles as the carriers for the pulmonary delivery of the SLNs, including the investigations of their characteristics, aerodynamic properties, pharmacokinetics and pharmacodynamics. The spherical and hollow microparticles presented a size of 4.1 ± 0.1 μm and a low tap density of 0.175 ± 0.02 g/cm3. In addition, the microparticles showed a high aerosolization efficiency (emitted dose of 98.0% ± 1.23% and respirable fraction of 51.07% ± 1.21%). Furthermore, the SLNs could be easily recovered from the microparticles without essential changes on their characteristics and the drug release behavior. The pharmacokinetic and pharmacodynamic studies suggested that, compared to i.v. TP5 solution, the bioavailability and therapeutic efficacy of TP5 were remarkably strengthened after the pulmonary administration of the microparticles. Taken together, we believe the microparticles were suitable for inhalation and possesed an ample potential for the pulmonary delivery of the SLNs.
Longxia Guo - One of the best experts on this subject based on the ideXlab platform.
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facile preparation of superparamagnetic surface imprinted microspheres using amino acid as template for specific capture of Thymopentin
Applied Surface Science, 2015Co-Authors: Longxia Guo, Ping Guan, Dan Wang, Dongmen Song, Xumian Gao, Renyuan SongAbstract:Abstract Novel superparamagnetic surface-imprinted microspheres (SIMs) with molecularly imprinted shell layer were controllably synthesized via fragment imprinting and surface imprinting technique. The SIMs-Arg and SIMs-Lys microspheres were prepared by using L-arginine (L-Arg) and L-lysine (L-Lys) as pseudo-template molecule for specific rebinding to Thymopentin (TP5), respectively. The characterization results revealed that both SIMs-Arg and SIMs-Lys were successfully prepared and possessed a high magnetic sensitivity. The rebinding-isotherm analyses of SIMs-Arg and SIMs-Lys showed that the Langmuir isotherm model was well fitted to the equilibrium data, indicating that only one kind of rebinding site was present in SIMs-Arg and SIMs-Lys. Besides, the kinetic properties of SIMs-Arg and SIMs-Lys both were well described by the pseudo-second-order kinetics model, which indicated that a chemical process may be the rate-limiting step in the rebinding process. Moreover, the magnetic imprinted microspheres were found to have a higher specificity for TP5 than that for immunostimulating peptide human (IPH). What is more, SIMs-Arg and SIMs-Lys were successfully applied for TP5 determination in urine. According to the maximum adsorption capacity, the imprinting factor and real sample experiment, it was noted that SIMs-Arg had better specific adsorption property for TP5 than SIMs-Lys.
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water compatible surface imprinted microspheres for high adsorption and selective recognition of peptide drug from aqueous media
Journal of Materials Chemistry B, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Chaoli WangAbstract:Novel water-compatible ionic liquid-functionalized microspheres with molecularly imprinted shell layer were controllably synthesized via precipitation polymerization and surface imprinting technique. Here, a room-temperature ionic liquid was synthesized to prepare these surface-imprinted microspheres with excellent water solubility and multiple binding sites with template molecules. The peptide drug Thymopentin (TP5) was chosen as a template molecule, which is known as an immunomodulating agent. The as-prepared microspheres were fully characterized. Results reveal that ionic liquid incorporation significantly improves the adsorption of TP5. Moreover, the adsorption property and recognition capability towards TP5 are closely related to the synergetic effect of electrostatic interaction and hydrogen bonding. Through employing the synergetic effect of directional and non-directional interactions, the surface-imprinted microspheres exhibit high adsorption capacity, good selective recognition, and rapid binding ability for TP5. The surface-imprinted microspheres demonstrate potential usage for TP5 enrichment from other biomolecules, and the proposed method was successfully applied for TP5 determination in Thymopentin injection and urine.
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synthesis of water compatible surface imprinted composite microspheres with core shell structure for selective recognition of Thymopentin from aqueous solution
Journal of Materials Science, 2015Co-Authors: Ping Guan, Liwei Qian, Renyuan Song, Longxia Guo, Yimei TangAbstract:A novel water-compatible surface-imprinted core–shell microsphere, which had multiple non–covalent interactions with template molecule, was successfully prepared by the surface grafting polymerization method in acetonitrile–water systems with Thymopentin as template through ionic liquid-functionalized polyethyleneglycolmethacrylate-co-vinylimidazole microsphere as the matrix. The average diameter of matrix was 1 μm ± 20 nm and the thickness of imprinted layer was about 50 nm. The results of static adsorption experiments indicated that ionic liquid-functionalized molecularly imprinted microspheres showed the good adsorption capacity and specific recognition for template peptide. The binding-isotherm analysis showed that Langmuir isotherm models gave a good fit in the range of concentrations, suggesting that there was only one kind of binding site in imprinted layer. Measurements of the binding kinetics revealed that surface-imprinted composite microspheres reached peptide-adsorption equilibrium in 60 min and the maximum adsorption capacity for TP5 was 38.4 mg g−1. The effects of pH, salt concentration, and temperature on the adsorption capacities were investigated. The microspheres were found to have a high specificity for TP5 with little affinity for BSA and Hb. Finally, the core–shell microspheres can be reused with only 15.6 % decrease in TP5 adsorption capacity after six times.