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Maciej Zabel - One of the best experts on this subject based on the ideXlab platform.

  • The effect of calcitriol and its analogues on proliferation and hormone expression in cultured cells of Thyroid Medullary Carcinomas
    Folia morphologica, 2003
    Co-Authors: Maciej Zabel, Krzysztof Flig, Elzbieta Gebarowska, Joanna Surdyk-zasada
    Abstract:

    The study aimed at evaluating the effects of calcitriol and of its analogues on the proliferation of TT and rMTC cells (human and rat line tumour cells originating from Thyroid Medullary Carcinoma) and at examining the effects of the substances on the secretion of the principal hormones of the cells, calcitonin (CT) and calcitonin gene-related peptide (CGRP). Cells of Thyroid Medullary Carcinoma (human TT cells and rat rMTC cells) were cultured for 5 days in the absence or in the presence of calcitriol and of its two analogues (PRI-1906 and PRI-2191) in concentrations of 10 -9 to 10 -6 M. Calcitriol and the applied analogues weakly inhibited proliferation of Thyroid Medullary Carcinoma in in vitro conditions. The evident effect of analogues on hormone secretion points to their effect on the process of CT gene expression.

  • Effect of calcitriol on proliferation of TT cells and on expression of calcitonin gene.
    Folia histochemica et cytobiologica, 2002
    Co-Authors: Maciej Zabel, Elzbieta Gebarowska, Malgorzata Drag-zalesinska, Teresa Wysocka
    Abstract:

    The absence of serum steroids was demonstrated to restrict proliferation of cultured TT cells (cell line originating from human Thyroid Medullary Carcinoma) and supplementation with calcitriol was found to partially restore the proliferation. Calcitriol stimulated TT cell proliferation by augmenting expression of proliferation-associated proteins and by restricting apoptosis. Moreover, calcitriol decreased the intensity of transcription but failed to change direction of the altemate splicing of the calcitonin gene.

  • Effect of octreotide on proliferation of in vitro cultured Thyroid Medullary Carcinoma cells.
    Folia histochemica et cytobiologica, 2001
    Co-Authors: Maciej Zabel, Elzbieta Gebarowska, Malgorzata Drag-zalesinska, Teresa Wysocka, Flig K
    Abstract:

    In TT cells, originating from Medullary Carcinoma of the human Thyroid, the presence of receptors for somatostatin was demonstrated at the ultrastructural level. Inhibitory effect of octreotide (a somatostatin analogue) was observed on proliferation of in vitro cultured TT cells and confirmed by evaluating levels of PCNA and Ki-67 proliferation-associated antigens and examining the extent of DNA damage using the comet assay. Our studies indicate a potential for application of somatostatin analogues to diagnosis and adjunct treatment in Thyroid Medullary Carcinomas.

  • Immunocytochemical characterization of two Thyroid Medullary Carcinoma cell linesin vitro
    The Histochemical Journal, 1995
    Co-Authors: Maciej Zabel, Jolanta Seidel, Aldona Kaczmarek, Joanna Surdyk-zasada, Jan Grzeszkowiak, Aleksander Górny
    Abstract:

    The immunocytochemical characterization of cell lines originating from Thyroid Medullary Carcinoma, i.e. human TT cells and rat rMTC 6-23 cells, was undertaken. The immunocytochemical studies were supplemented by ultrastructural studies, including ultrastructural immunocytochemistry, and by radioimmunological estimation of calcitonin secretion to the medium. In rMTC 6-23 cells (subcultures 24 to 30), no hormone presence was demonstrated immunocytochemically, which corresponded to the absence of secretory granules at the ultrastructural level. Of various proteins sought, only neuron-specific enolase could be demonstrated. Nevertheless, the cells secreted calcitonin into the medium. TT cells (passages 145 to 160) produced secretory granules. The granules contained calcitonin, calcitonin gene-related peptide, somatostatin, neurotensin, met-enkephalin, leu-enkephalin, gastrin releasing peptide, paraThyroid hormone-related protein, functional proteins of the chromogranin group and synaptophysin. Other functional proteins found in the cytosol of TT cells included non-specific enolase, calbindin and tyrosine hydroxylase. Receptor for calcitriol was localized in the cell nucleus. Marker proteins were localized in the cytosol (carcinoembryonic antigen) and in the cell skeleton (α-tubulin, cytokeratin). Following changes in ionized calcium levels in the medium, changes in calcitonin secretion and in immunocytochemical detectability of some hormones and functional proteins were observed. TT cells demonstrated the expression of numerous hormones and functional proteins associated with calcitonin secretion. Further, the cells in their ultrastructure, immunocytochemical and secretory characteristics, resemble more closely normal parafollicular cells of the Thyroid and, in our opinion, represent a more appropriate model for functional studies.

  • Immunocytochemical characterization of two Thyroid Medullary Carcinoma cell lines in vitro
    The Histochemical Journal, 1995
    Co-Authors: Maciej Zabel, Jolanta Seidel, Aldona Kaczmarek, Joanna Surdyk-zasada, Jan Grzeszkowiak, Aleksander Górny
    Abstract:

    The immunocytochemical characterization of cell lines originating from Thyroid Medullary Carcinoma, i.e. human TT cells and rat rMTC 6-23 cells, was undertaken. The immunocytochemical studies were supplemented by ultrastructural studies, including ultrastructural immunocytochemistry, and by radioimmunological estimation of calcitonin secretion to the medium. In rMTC 6-23 cells (subcultures 24 to 30), no hormone presence was demonstrated immunocytochemically, which corresponded to the absence of secretory granules at the ultrastructural level. Of various proteins sought, only neuron-specific enolase could be demonstrated. Nevertheless, the cells secreted calcitonin into the medium. TT cells (passages 145 to 160) produced secretory granules. The granules contained calcitonin, calcitonin gene-related peptide, somatostatin, neurotensin, met-enkephalin, leu-enkephalin, gastrin releasing peptide, paraThyroid hormone-related protein, functional proteins of the chromogranin group and synaptophysin. Other functional proteins found in the cytosol of TT cells included non-specific enolase, calbindin and tyrosine hydroxylase. Receptor for calcitriol was localized in the cell nucleus. Marker proteins were localized in the cytosol (carcinoembryonic antigen) and in the cell skeleton (α-tubulin, cytokeratin). Following changes in ionized calcium levels in the medium, changes in calcitonin secretion and in immunocytochemical detectability of some hormones and functional proteins were observed. TT cells demonstrated the expression of numerous hormones and functional proteins associated with calcitonin secretion. Further, the cells in their ultrastructure, immunocytochemical and secretory characteristics, resemble more closely normal parafollicular cells of the Thyroid and, in our opinion, represent a more appropriate model for functional studies.

Satylganov Izh - One of the best experts on this subject based on the ideXlab platform.

  • Histological variants of Thyroid Medullary Carcinoma
    Arkhiv patologii, 2001
    Co-Authors: Satylganov Izh
    Abstract:

    A total of 114 Medullary Carcinomas of the Thyroid were studied clinically and histologically. Females and subjects aged over 40 years predominated among the patients (2-fold more than others). Five- and ten-year survival was 74 and 46.2%, respectively. The histological variants were as follows: solid, follicular, papillary, small-cell, clear-cell, oncocytic, and mixed Medullary-follicular and Medullary-papillary. The survival of these patients did not depend on the histological variant or the degree of tumor cell atypia.

  • Ultrastructural characteristics of Thyroid Medullary Carcinoma
    Arkhiv patologii, 2001
    Co-Authors: Satylganov Izh
    Abstract:

    17 Thyroid Medullary Carcinomas (TMC) were studied electron microscopically (EM). Two cell groups were revealed regardless of their histological structure: EM differentiated (with signs of C-cell differentiation) and non-differentiated (without these signs). There is no correlation between histological structure of the tumor and the degree of its EM differentiation. In patients who lived 5 years maximum after the diagnosis there were different histological types but EM the tumors had relatively monomorphous structure with domination of non-differentiated cells (more than 70%), high content of dark cells (50% and more) and low content of desmosomes (less than 30%). The prognosis is more favourable the higher is content of EM differentiated cells, the better desmosomes are developed and the lower is content of dark cells. The scope of surgery and local metastases do not influence the prognosis while distant metastases (stage IV) deteriorate the prognosis.

  • Spindle-cell variant of Thyroid Medullary Carcinoma with favourable prognosis
    Arkhiv patologii, 2000
    Co-Authors: Satylganov Izh
    Abstract:

    A case of a favourable outcome of spindle-cell Medullary Carcinoma of the Thyroid is reported. Electron-microscopically, there was no correlation between histological and ultrastructural differentiation. The tumor consisted mainly of ultrastructurally differentiated cells with endocrine granules which may be the cause of the favourable prognosis.

Carmen Martinez - One of the best experts on this subject based on the ideXlab platform.

  • Survival from rare cancer in adults: a population-based study.
    Lancet Oncology, 2006
    Co-Authors: Gemma Gatta, Laura Ciccolallo, Ian Kunkler, Riccardo Capocaccia, Franco Berrino, Michel P Coleman, Roberta De Angelis, Jean Faivre, Jean Michel Lutz, Carmen Martinez
    Abstract:

    BACKGROUND: Rare cancers are a challenge to clinical practice, and treatment experience, even in major cancer centres to which rare cancers are usually referred, is often limited. We aimed to study the epidemiology of rare cancers in a large population of several countries. METHODS: We analysed survival by age, sex, subsite, and morphology in 57,144 adults with 14 selected rare cancers diagnosed 1983-94. Variations in survival over time and between European regions were also assessed for variations in quality of care. We also estimated the adjusted relative excess risk of death for every rare cancer. FINDINGS: Overall 5-year relative survival was good (ie, >65%) for placental chorioCarcinoma (85.4% [95% CI 81.4-89.5]), Thyroid Medullary Carcinoma (72.4% [69.2-75.5]), ovarian germ-cell cancer (73.0% [70.0-76.0]), lung carcinoid (70.1% [67.3-72.9]), and cervical adenoCarcinoma (65.5% [64.3-66.6]); intermediate (ie, 35-65%) for testicular cancer at age 65 years or older (64.0% [59.3-68.7]), sarcoma of extremities (60.0% [58.9-61.2]), digestive-system endocrine cancers (55.6% [54.9-56.3]), anal squamous-cell Carcinoma (53.1% [51.5-54.8]), and uterine sarcoma (43.5% [42.0-44.9]); low for Carcinoma of adrenal-gland cortex (32.7% [28.3-37.2]) and bladder squamous-cell Carcinoma (20.4% [18.8-22.0]); and poor for angiosarcoma of liver (6.4% [1.8-11.0]) and mesothelioma (4.7% [4.3-5.2]). Survival was usually better for women than men and poor in those aged 75 years or older. Survival significantly improved over time for ovarian germ-cell cancer, sarcomas of extremities, digestive-system endocrine tumours, anal squamous-cell Carcinoma, and angiosarcoma of liver. Survival in northern Europe was higher than in the other geographic groupings for most cancers. INTERPRETATION: Because effective treatments are available for several of the rare cancers we assessed, further research is needed to ascertain why survival is lower in some European countries than in others, particularly in older patients. Audit of best practice for rare cancers with treatment protocols would be useful.

Joanna Surdyk-zasada - One of the best experts on this subject based on the ideXlab platform.

  • The effect of calcitriol and its analogues on proliferation and hormone expression in cultured cells of Thyroid Medullary Carcinomas
    Folia morphologica, 2003
    Co-Authors: Maciej Zabel, Krzysztof Flig, Elzbieta Gebarowska, Joanna Surdyk-zasada
    Abstract:

    The study aimed at evaluating the effects of calcitriol and of its analogues on the proliferation of TT and rMTC cells (human and rat line tumour cells originating from Thyroid Medullary Carcinoma) and at examining the effects of the substances on the secretion of the principal hormones of the cells, calcitonin (CT) and calcitonin gene-related peptide (CGRP). Cells of Thyroid Medullary Carcinoma (human TT cells and rat rMTC cells) were cultured for 5 days in the absence or in the presence of calcitriol and of its two analogues (PRI-1906 and PRI-2191) in concentrations of 10 -9 to 10 -6 M. Calcitriol and the applied analogues weakly inhibited proliferation of Thyroid Medullary Carcinoma in in vitro conditions. The evident effect of analogues on hormone secretion points to their effect on the process of CT gene expression.

  • Immunocytochemical characterization of two Thyroid Medullary Carcinoma cell linesin vitro
    The Histochemical Journal, 1995
    Co-Authors: Maciej Zabel, Jolanta Seidel, Aldona Kaczmarek, Joanna Surdyk-zasada, Jan Grzeszkowiak, Aleksander Górny
    Abstract:

    The immunocytochemical characterization of cell lines originating from Thyroid Medullary Carcinoma, i.e. human TT cells and rat rMTC 6-23 cells, was undertaken. The immunocytochemical studies were supplemented by ultrastructural studies, including ultrastructural immunocytochemistry, and by radioimmunological estimation of calcitonin secretion to the medium. In rMTC 6-23 cells (subcultures 24 to 30), no hormone presence was demonstrated immunocytochemically, which corresponded to the absence of secretory granules at the ultrastructural level. Of various proteins sought, only neuron-specific enolase could be demonstrated. Nevertheless, the cells secreted calcitonin into the medium. TT cells (passages 145 to 160) produced secretory granules. The granules contained calcitonin, calcitonin gene-related peptide, somatostatin, neurotensin, met-enkephalin, leu-enkephalin, gastrin releasing peptide, paraThyroid hormone-related protein, functional proteins of the chromogranin group and synaptophysin. Other functional proteins found in the cytosol of TT cells included non-specific enolase, calbindin and tyrosine hydroxylase. Receptor for calcitriol was localized in the cell nucleus. Marker proteins were localized in the cytosol (carcinoembryonic antigen) and in the cell skeleton (α-tubulin, cytokeratin). Following changes in ionized calcium levels in the medium, changes in calcitonin secretion and in immunocytochemical detectability of some hormones and functional proteins were observed. TT cells demonstrated the expression of numerous hormones and functional proteins associated with calcitonin secretion. Further, the cells in their ultrastructure, immunocytochemical and secretory characteristics, resemble more closely normal parafollicular cells of the Thyroid and, in our opinion, represent a more appropriate model for functional studies.

  • Immunocytochemical characterization of two Thyroid Medullary Carcinoma cell lines in vitro
    The Histochemical Journal, 1995
    Co-Authors: Maciej Zabel, Jolanta Seidel, Aldona Kaczmarek, Joanna Surdyk-zasada, Jan Grzeszkowiak, Aleksander Górny
    Abstract:

    The immunocytochemical characterization of cell lines originating from Thyroid Medullary Carcinoma, i.e. human TT cells and rat rMTC 6-23 cells, was undertaken. The immunocytochemical studies were supplemented by ultrastructural studies, including ultrastructural immunocytochemistry, and by radioimmunological estimation of calcitonin secretion to the medium. In rMTC 6-23 cells (subcultures 24 to 30), no hormone presence was demonstrated immunocytochemically, which corresponded to the absence of secretory granules at the ultrastructural level. Of various proteins sought, only neuron-specific enolase could be demonstrated. Nevertheless, the cells secreted calcitonin into the medium. TT cells (passages 145 to 160) produced secretory granules. The granules contained calcitonin, calcitonin gene-related peptide, somatostatin, neurotensin, met-enkephalin, leu-enkephalin, gastrin releasing peptide, paraThyroid hormone-related protein, functional proteins of the chromogranin group and synaptophysin. Other functional proteins found in the cytosol of TT cells included non-specific enolase, calbindin and tyrosine hydroxylase. Receptor for calcitriol was localized in the cell nucleus. Marker proteins were localized in the cytosol (carcinoembryonic antigen) and in the cell skeleton (α-tubulin, cytokeratin). Following changes in ionized calcium levels in the medium, changes in calcitonin secretion and in immunocytochemical detectability of some hormones and functional proteins were observed. TT cells demonstrated the expression of numerous hormones and functional proteins associated with calcitonin secretion. Further, the cells in their ultrastructure, immunocytochemical and secretory characteristics, resemble more closely normal parafollicular cells of the Thyroid and, in our opinion, represent a more appropriate model for functional studies.

Elzbieta Gebarowska - One of the best experts on this subject based on the ideXlab platform.