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Peter Laurberg - One of the best experts on this subject based on the ideXlab platform.
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serum tsh and serum Thyroid Peroxidase Antibody fluctuate in parallel and high urinary iodine excretion predicts subsequent Thyroid failure in a 1 year study of patients with untreated subclinical hypoThyroidism
European Journal of Endocrinology, 2008Co-Authors: Jesper Karmisholt, Peter LaurbergAbstract:Objective: To explore the possibility of predicting decline or improvement in Thyroid function over 1 year, and to investigate the correlations of serum TSH (s-TSH) with hypoThyroidism-related symptoms and signs, serum Thyroid Peroxidase Antibody (s-TPO-Ab) and urinary iodine excretion in individual patients with untreated subclinical hypoThyroidism (SH). Design: Monthly repeated measurement study without intervention. Methods:Twenty-onepatientswithoutformerThyroiddiseasewhohadbeenidentifiedwiths-TSHbetween 5 and 12 mU/l and normal serum thyroxine (s-T4) at two occasions were enrolled. Subsequently, 13 monthly measurements of s-TSH, hypoThyroidism-related symptoms and signs, serum free T4 ,s -TPO-Ab and urinary iodine excretion were performed. Results:Overthestudyyear,s-TSHincreasedsignificantlyin5patients,16hadunchangeds-TSH,whereas none improved. From clinical and biochemical inclusion data, it was not possible to predict who would later increase in s-TSH. In individual patients, a highly significant correlation between s-TSH and s-TPO-Ab was found (rZ0.37, P!0.0001) and also between s-TSH and urinary iodine excretion (rZ 0.14,PZ0.034).Nocorrelationbetweens-TSHandclinicalsymptomsandsignswasobserved.Timeshift showedbestcorrelation between s-TSH and s-TPO-Ab measuredatthesametimepoint, whereasurinary iodine excretion correlated best to s-TSH and s-TPO-Ab obtained 1 month later. Conclusion:Atthetimeofinclusion,itwasnotpossibletoidentifythe24%ofSHpatientswhowouldshow deteriorationinThyroidfunctionoverthefollowingyear.ImpairmentinThyroidfunctionvariedinparallel with Thyroid autoimmunity,whereas high urinary iodine excretionpredicted highs-TSHand s-TPO-Ab 1 month later.
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postpartum Thyroid dysfunction in pregnant Thyroid Peroxidase Antibody positive women living in an area with mild to moderate iodine deficiency is iodine supplementation safe
The Journal of Clinical Endocrinology and Metabolism, 2000Co-Authors: Susanne Nohr, Annemette Jorgensen, Klaus M Pedersen, Peter LaurbergAbstract:In moderately iodine-deficient, pregnant, Thyroid Peroxidase Antibody (TPO-Ab)-positive women the role of iodine supplementation in the development of postpartum Thyroid dysfunction (PPTD) was studied in a placebo-controlled, randomized, double blind trial. Screening for TPO-Ab was performed in early pregnancy in a population of healthy pregnant Danish women with no previous diagnosed Thyroid disease (prevalence, 117 of 1284; 9.1%). The participants were randomized, stratified according to TPO-Ab level, to three groups. All participants received a daily vitamin and mineral tablet with 150 μg iodine or no iodine. The +/+ group received iodine during pregnancy and the postpartum period, the +/− group received iodine during pregnancy only, and the −/− group received no iodine supplementation. A total of 66 TPO-Ab positive women were followed, and in the postpartum period sera were collected at 8-week interval for biochemical evaluation of Thyroid function and Antibody level. Compliance was evaluated by 24-h ...
Guizhi Lu - One of the best experts on this subject based on the ideXlab platform.
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glycosylation of recombinant human Thyroid Peroxidase ectodomain of insect cell origin has little effect on recognition by serum Thyroid Peroxidase Antibody
Chinese Medical Journal, 2013Co-Authors: Qing Li, Lanlan Zhao, Youyuan Huang, Guizhi LuAbstract:BACKGROUND: Thyroid Peroxidase (TPO) is an important autoantigen in Hashimoto's Thyroiditis (HT), and almost all epitopes are located in TPO ectodomain. The glycosylation of TPO might contribute to breaking self-tolerance, therefore, purified glycosylated recombinant TPO ectodomain is prerequisite of elucidating its role in the pathogenesis of HT. The aim of our study was to investigate whether the glycosylation has influence on the antigenic determinants of recombinant TPO. METHODS: Bac-to-Bac baculovirus expression system was used to generate recombinant human TPO ectodomain. The antigenicity was analyzed by antigen specific enzyme-linked immunosorbant assays (ELISAs). The glycosylation of recombinant human TPO ectodomain of High Five insect cell origin was detected by lectin-ELISAs. RESULTS: TPO ectodomain was recovered from the culture media as a soluble protein, and it was fused with a hexahistidine tag which allowed purification by nickel-affinity chromatography. The recombinant TPO ectodomain could be recognized by all the 54 HT patients and three TPO monoclonal antibodies. Fucose, sialic acid and galactose were all detected on the recombinant TPO ectodomain. Sera TPOAb binding decreased slightly after non-specific deglycosylation of TPO by periodic acid. CONCLUSIONS: High Five insect cells derived recombinant human TPO ectodomain had N-glycosylation sites, which might have little effect on recognition by serum TPOAb.
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distribution of immunoglobulin g subclasses of anti Thyroid Peroxidase Antibody in sera from patients with hashimoto s Thyroiditis with different Thyroid functional status
Clinical and Experimental Immunology, 2008Co-Authors: Mengtao Li, Guizhi LuAbstract:Summary The mechanism of disease progression in Hashimoto's Thyroiditis (HT) is still unclear. Anti-Thyroid Peroxidase Antibody (TPOAb), a diagnostic hallmark of HT, is principally of the immunoglobulin G (IgG) isotype, and it appears to be a response to Thyroid injury. The aim of our study was to evaluate the distribution of IgG subclasses of TPOAb in sera from patients with HT with different Thyroid functional status. Sera from 168 patients with newly diagnosed HT were collected and divided into three groups according to Thyroid function: patients with hypoThyroidism (n = 66), subclinical hypoThyroidism (n = 60) and euThyroidism (n = 42). Antigen-specific enzyme-linked immunosorbent assay was used to detect the distribution of TPOAb IgG subclasses. The prevalence of TPOAb IgG subclasses in all patients’ sera with HT was IgG1 70·2%, IgG2 35·1%, IgG3 19·6% and IgG4 66·1% respectively. The prevalence of IgG2 in sera from patients with hypoThyroidism (51·5%) was significantly higher than that of subclinical hypoThyroidism (33·3%) (P < 0·05), and the latter was also significantly higher than that of euThyroidism (11·9%) (P < 0·05). The positive percentage of IgG2 subclass in sera from patients with hypoThyroidism and subclinical hypoThyroidism was significantly higher than that of euThyroidism (P < 0·05), the prevalence and positive percentage of IgG4 subclass in sera from patients with hypoThyroidism and subclinical hypoThyroidism was significantly higher than that of euThyroidism respectively (P < 0·05). The predominant TPOAb IgG subclasses in sera from patients with HT were IgG1 and IgG4. Patients with high levels of TPOAb IgG2, IgG4 subclasses might be at high risk of developing overt hypoThyroidism.
Baptist Gallwitz - One of the best experts on this subject based on the ideXlab platform.
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Thyroid Peroxidase Antibody positivity is associated with symptomatic distress in patients with hashimoto s Thyroiditis
Brain Behavior and Immunity, 2012Co-Authors: Karsten Müssig, Andreas Künle, Anna-laura Säuberlich, Claudia Weinert, Thomas Ethofer, Ralf Saur, Reinhild Klein, Hans-ulrich Häring, Stefan Klingberg, Baptist GallwitzAbstract:Abstract Previous studies suggest impairments of physical, mental, and psychic well-being in patients with Hashimoto’s Thyroiditis (HT), but these impairments have been shown to be independent of Thyroid dysfunction. In 64 euThyroid patients with HT, symptomatic distress was assessed with the Symptom Checklist-90-Revised (SCL-90-R), a 90-item multidimensional self-report symptom inventory using a 5-point rating scale. In a subgroup of patients, endocrine testing 3 years prior to the current investigation was available. Anti-Thyroid Peroxidase antibodies (TPO-Abs) were associated with the three SCL-90-R global indices Global Severity Index (GSI), Positive Symptom Distress Index (PSDI), and Positive Symptom Total (PST) as well as with somatization and obsessive–compulsive symptoms after adjustment for age, gender, and Thyroid function as assessed by TSH levels (all p p ⩽ 0.02), though the aforementioned associations did not withstand sequential Bonferroni correction for multiple testing. In contrast, TPO-Abs positivity, defined as TPO-Abs >100 IU/l, significantly predicted poorer psychosocial well-being in all of the three SCL-90-R global indices after three years, even after correction (all p ⩽ 0.02). In conclusion, high TPO-Abs are associated with poor physical and psychological well-being and appear to predict future health perception in HT patients.
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Thyroid Peroxidase Antibody positivity is associated with symptomatic distress in patients with Hashimoto’s Thyroiditis
Brain Behavior and Immunity, 2012Co-Authors: Karsten Müssig, Andreas Künle, Anna-laura Säuberlich, Claudia Weinert, Thomas Ethofer, Ralf Saur, Reinhild Klein, Hans-ulrich Häring, Stefan Klingberg, Baptist GallwitzAbstract:Abstract Previous studies suggest impairments of physical, mental, and psychic well-being in patients with Hashimoto’s Thyroiditis (HT), but these impairments have been shown to be independent of Thyroid dysfunction. In 64 euThyroid patients with HT, symptomatic distress was assessed with the Symptom Checklist-90-Revised (SCL-90-R), a 90-item multidimensional self-report symptom inventory using a 5-point rating scale. In a subgroup of patients, endocrine testing 3 years prior to the current investigation was available. Anti-Thyroid Peroxidase antibodies (TPO-Abs) were associated with the three SCL-90-R global indices Global Severity Index (GSI), Positive Symptom Distress Index (PSDI), and Positive Symptom Total (PST) as well as with somatization and obsessive–compulsive symptoms after adjustment for age, gender, and Thyroid function as assessed by TSH levels (all p p ⩽ 0.02), though the aforementioned associations did not withstand sequential Bonferroni correction for multiple testing. In contrast, TPO-Abs positivity, defined as TPO-Abs >100 IU/l, significantly predicted poorer psychosocial well-being in all of the three SCL-90-R global indices after three years, even after correction (all p ⩽ 0.02). In conclusion, high TPO-Abs are associated with poor physical and psychological well-being and appear to predict future health perception in HT patients.
Jesper Karmisholt - One of the best experts on this subject based on the ideXlab platform.
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serum tsh and serum Thyroid Peroxidase Antibody fluctuate in parallel and high urinary iodine excretion predicts subsequent Thyroid failure in a 1 year study of patients with untreated subclinical hypoThyroidism
European Journal of Endocrinology, 2008Co-Authors: Jesper Karmisholt, Peter LaurbergAbstract:Objective: To explore the possibility of predicting decline or improvement in Thyroid function over 1 year, and to investigate the correlations of serum TSH (s-TSH) with hypoThyroidism-related symptoms and signs, serum Thyroid Peroxidase Antibody (s-TPO-Ab) and urinary iodine excretion in individual patients with untreated subclinical hypoThyroidism (SH). Design: Monthly repeated measurement study without intervention. Methods:Twenty-onepatientswithoutformerThyroiddiseasewhohadbeenidentifiedwiths-TSHbetween 5 and 12 mU/l and normal serum thyroxine (s-T4) at two occasions were enrolled. Subsequently, 13 monthly measurements of s-TSH, hypoThyroidism-related symptoms and signs, serum free T4 ,s -TPO-Ab and urinary iodine excretion were performed. Results:Overthestudyyear,s-TSHincreasedsignificantlyin5patients,16hadunchangeds-TSH,whereas none improved. From clinical and biochemical inclusion data, it was not possible to predict who would later increase in s-TSH. In individual patients, a highly significant correlation between s-TSH and s-TPO-Ab was found (rZ0.37, P!0.0001) and also between s-TSH and urinary iodine excretion (rZ 0.14,PZ0.034).Nocorrelationbetweens-TSHandclinicalsymptomsandsignswasobserved.Timeshift showedbestcorrelation between s-TSH and s-TPO-Ab measuredatthesametimepoint, whereasurinary iodine excretion correlated best to s-TSH and s-TPO-Ab obtained 1 month later. Conclusion:Atthetimeofinclusion,itwasnotpossibletoidentifythe24%ofSHpatientswhowouldshow deteriorationinThyroidfunctionoverthefollowingyear.ImpairmentinThyroidfunctionvariedinparallel with Thyroid autoimmunity,whereas high urinary iodine excretionpredicted highs-TSHand s-TPO-Ab 1 month later.
John Burgess - One of the best experts on this subject based on the ideXlab platform.
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temporal trends in Thyroid stimulating hormone tsh and Thyroid Peroxidase Antibody atpo testing across two phases of iodine fortification in tasmania 1995 2013
Clinical Endocrinology, 2017Co-Authors: A Hong, B Stokes, Petr Otahal, D Owens, John BurgessAbstract:Context: Tasmania is an island state of the Australian Commonwealth with a welldocumented history of mild iodine deficiency. Between 2001 and 2009, Tasmania experienced two incremental phases of iodine fortification. Objective: To examine trends in Thyroid-stimulating hormone (TSH) and Thyroid Peroxidase Antibody (ATPO) testing and their relationship to different phases of iodine nutrition in the Tasmanian population between 1995 and 2013. Design: Retrospective longitudinal study. Setting and participants: The major primary care and largest public hospital pathology providers in Tasmania submitted data for all TSH and ATPO tests performed between 1995 and 2013. Data linkage methodology was used to determine trends in TSH and ATPO testing. Results: A total of 1.66 million TSH assessments, involving 389,910 individual patients, were performed in Tasmania between 1995 and 2013. There was approximately a fourfold increase in the overall rate of TSH testing during this period with the rate of incident TSH assessment remaining relatively stable over the study period. The incidence of overt suppression and elevation of TSH (TSH≤0.1 mIU/L and ≥10 mIU/L) declined 62.3% and 59.7%, respectively, with a trend for increased incidence of borderline TSH elevation ≥4.0 mIU/L. The incidence of Thyroid autoimmunity as determined by the proportion of abnormal ATPO results remained stable, with the absolute number of positive test results increasing during the study period. Conclusion: Iodine supplementation of this mildly iodine-deficient population was not associated with an obvious increase in incidence of overt Thyroid dysfunction or autoimmunity. Whilst the volume of TSH testing increased over the study period, the increase was driven by patients undergoing follow-up TSH assessments.