The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Libor Grubhoffer - One of the best experts on this subject based on the ideXlab platform.
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CD8+ T-cells mediate immunopathology in Tick-Borne Encephalitis.
Virology, 2008Co-Authors: Daniel Růžek, T.s. Gritsun, Ernest A. Gould, Jan Kopecky, Jiří Salát, Martin Palus, Iva Dyková, Anna Skallová, Jiří Jelínek, Libor GrubhofferAbstract:Epidemics of Tick-Borne Encephalitis involving thousands of humans occur annually in the forested regions of Europe and Asia. Despite the importance of this disease, the underlying basis for the development of Encephalitis remains undefined. Here, we prove the key role of CD8+ T-cells in the immunopathology of Tick-Borne Encephalitis, as demonstrated by prolonged survival of SCID or CD8−/− mice, following infection, when compared with immunocompetent mice or mice with adoptively transferred CD8+ T-cells. The results imply that Tick-Borne Encephalitis is an immunopathological disease and that the inflammatory reaction significantly contributes to the fatal outcome of the infection.
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Rapid subtyping of Tick-Borne Encephalitis virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in Ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis virus.
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Rapid subtyping of Tick-Borne Encephalitis virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in Ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis virus.
Jan Kopecky - One of the best experts on this subject based on the ideXlab platform.
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CD8+ T-cells mediate immunopathology in Tick-Borne Encephalitis.
Virology, 2008Co-Authors: Daniel Růžek, T.s. Gritsun, Ernest A. Gould, Jan Kopecky, Jiří Salát, Martin Palus, Iva Dyková, Anna Skallová, Jiří Jelínek, Libor GrubhofferAbstract:Epidemics of Tick-Borne Encephalitis involving thousands of humans occur annually in the forested regions of Europe and Asia. Despite the importance of this disease, the underlying basis for the development of Encephalitis remains undefined. Here, we prove the key role of CD8+ T-cells in the immunopathology of Tick-Borne Encephalitis, as demonstrated by prolonged survival of SCID or CD8−/− mice, following infection, when compared with immunocompetent mice or mice with adoptively transferred CD8+ T-cells. The results imply that Tick-Borne Encephalitis is an immunopathological disease and that the inflammatory reaction significantly contributes to the fatal outcome of the infection.
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Rapid subtyping of Tick-Borne Encephalitis virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in Ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis virus.
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Rapid subtyping of Tick-Borne Encephalitis virus isolates using multiplex RT-PCR.
Journal of Virological Methods, 2007Co-Authors: Hana Šťastná, Irina Golovljova, Jan Kopecky, Libor GrubhofferAbstract:Tick-Borne Encephalitis virus, an emerging pathogen in several countries in Europe and Asia, has been divided into three subtypes (European, Siberian and Far Eastern). These subtypes are associated with different severities of the disease. For that reason, early determination of the subtype in a clinical sample or in Ticks removed from a patient in areas of co-circulation of two or three subtypes is of high importance. The development of a simple method of multiplex RT-PCR for rapid and easy subtyping of Tick-Borne Encephalitis virus isolates is reported to fill this requirement. The method is based on the unique combination of oligonucleotide primers hybridizing with subtype-specific "signature" positions of the sequence encoding the viral envelope protein. The developed multiplex RT-PCR also appears to be a useful method in studies focused on the molecular-epidemiology of Tick-Borne Encephalitis virus.
Ursula Kunze - One of the best experts on this subject based on the ideXlab platform.
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Tick-Borne Encephalitis: from childhood to golden age does increased mobility mean increased risk? Conference report of the 11th meeting of the International Scientific Working Group on Tick-Borne Encephalitis (ISW-TBE).
Vaccine, 2009Co-Authors: Ursula KunzeAbstract:Tick-Borne Encephalitis: From childhood to golden age does\ud increased mobility mean increased risk?\ud Conference report of the 11th meeting of the International Scientific Working Group on Tick-Borne Encephalitis (ISW-TBE
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Tick-Borne Encephalitis in the golden agers: A conference report of the International Scientific Working Group on Tick-Borne Encephalitis (ISW TBE)
Vaccine, 2006Co-Authors: Ursula Kunze, Franz X. Heinz, Ulf Baumhackl, Rudolf Bretschneider, Vaclav Chmelik, Beatrix Grubeck-loebenstein, Mats Haglund, Reinhard Kaiser, P. Kimmig, Christian KunzAbstract:The international consensus "Tick-Borne Encephalitis in childhood" was an important and successful achievement of the International Scientific Working Group on Tick-Borne Encephalitis (ISW TBE) [1]. The main topic of the 2005 conference meeting was "Tick-Borne Encephalitis in the golden agers". "Golden agers" is the synonym for people over 50 years of age. Still the incidence of TBE is underestimated, even in countries where the disease is known and there must be European regions where TBE occurs and is not yet diagnosed properly. Increasing mobility and the changes in lifestyle of people during the last 10 to 15 years, also in the age group over 50 years, have exacerbated the risk and the number of infections. In order to get more information about Tick-Borne Encephalitis in older ages, data of seven European countries were presented (Austria, Croatia, Czech Republic, Germany, Lithuania, Poland and Sweden). Based on the scientific findings adequate conclusions were drafted by the ISW TBE.
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Tick-Borne Encephalitis IN CHILDHOOD – CONSENSUS 2004
Slovenian Medical Journal, 2004Co-Authors: Ursula Kunze, Franz X. Heinz, Vaclav Chmelik, Reinhard Kaiser, Loreta Asokliene, Tagir Bektimirov, Andreas Busse, Volker Hingst, Ferenc Kadar, P. KimmigAbstract:Tick-Borne Encephalitis (TBE) is a communicable disease caused by a flavi-virus, Ticks being the main vectors. The nervous system is affected, four clinical features of different severity are observed: meningitis, meningoEncephalitis, meningoencephalomyelitis, meningoradiculoneuritis. TBE is a preventable disease, which is rapidly becoming a growing public health problem in Europe. So far, no causal treatment is possible but an efficient, safe vaccination is available. During the 6th meeting of the International Scientific Working Group on TBE with the main conference issue "Tick-Borne Encephalitis in childhood" an international consensus was achieved. In countries where TBE is endemic – and not prevented by immunization –, both children and adults are affected. The disease in children is generally milder, although severe illness may occur and even lead to permanent impairment of the quality of life due to neuropsychological sequelae. Therefore, immunization should be offered to all children living in or traveling to endemic areas.
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Tick-Borne Encephalitis in childhood--consensus 2004.
Wiener medizinische Wochenschrift (1946), 2004Co-Authors: Ursula Kunze, Franz X. Heinz, Vaclav Chmelik, Reinhard Kaiser, Loreta Asokliene, Tagir Bektimirov, Andreas Busse, Volker Hingst, Ferenc Kadar, Peter KimmigAbstract:Tick-Borne Encephalitis (TBE) is a communicable disease caused by a flavi-virus, Ticks being the main vectors. The nervous system is affected, four clinical features of different severity are observed: meningitis, meningoEncephalitis, meningoencephalomyelitis, meningoradiculoneuritis. TBE is a preventable disease, which is rapidly becoming a growing public health problem in Europe. So far no causal treatment is possible but an efficient, safe vaccination is available. During the 6th meeting of the International Scientific Working Group on TBE with the main conference issue "Tick-Borne Encephalitis in childhood" an international consensus was achieved. In countries where TBE is endemic--and not prevented by immunization--both children and adults are affected. The disease in children is generally milder, although severe illness may occur and even lead to permanent impairment of the quality of life due to neuropsychological sequelae. Therefore immunization should be offered to all children living in or traveling to endemic areas.
Tatjana Avšič-Županc - One of the best experts on this subject based on the ideXlab platform.
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Cross-neutralisation of viruses of the Tick-Borne Encephalitis complex following Tick-Borne Encephalitis vaccination and/or infection
npj Vaccines, 2017Co-Authors: Alexander J. Mcauley, Tatjana Avšič-Županc, Bevan Sawatsky, Thomas Ksiazek, Maricela Torres, Miša Korva, Stanka Lotrič-furlan, Veronika Von Messling, Michael R. Holbrook, Alexander N. FreibergAbstract:Prior exposure to Tick-Borne Encephalitis virus confers immunity to distinct, but genetically related, significant human pathogens. The Tick-Borne Encephalitis complex comprises multiple ‘flaviviruses’; however, a licensed vaccine only exists for one: Tick-Borne Encephalitis virus (TBEV). Alexander McAuley, of the University of Texas Medical Branch, USA, and collaborators tested human serum samples from donors who had either received the TBEV vaccine, been infected with the virus naturally, or had contracted the virus despite prior vaccination. The team tested the sera against six genetically related flaviviruses, looking for antibodies that indicate viral immunity. The results showed that TBEV exposure induce production of antibodies that inhibit other flaviviruses, the efficacy of which decreases as the genetic variation from TBEV increases. Additionally, sera from infected individuals generated more antibodies than sera from vaccinated donors. This study provides an insight into the relationship between genetic similarity and vaccine cross-protection. The Tick-Borne Encephalitis complex contains a number of flaviviruses that share close genetic homology, and are responsible for significant human morbidity and mortality with widespread geographical range. Although many members of this complex have been recognised for decades, licenced human vaccines with broad availability are only available for Tick-Borne Encephalitis virus. While Tick-Borne Encephalitis virus vaccines have been demonstrated to induce significant protective immunity, as determined by virus-neutralisation titres, vaccine breakthrough (clinical infection following complete vaccination), has been described. The aim of this study was to confirm the cross-neutralisation of Tick-Borne flaviviruses using mouse immune ascitic fluids, and to determine the magnitude of cross-neutralising antibody titres in sera from donors following Tick-Borne Encephalitis vaccination, infection, and vaccine breakthrough. The results demonstrate that there is significant cross-neutralisation of representative members of the Tick-Borne Encephalitis complex following vaccination and/or infection, and that the magnitude of immune responses varies based upon the exposure type. Donor sera successfully neutralised most of the viruses tested, with 85% of vaccinees neutralising Kyasanur forest disease virus and 73% of vaccinees neutralising Alkhumra virus. By contrast, only 63% of vaccinees neutralised Powassan virus, with none of these neutralisation titres exceeding 1:60. Taken together, the data suggest that Tick-Borne Encephalitis virus vaccination may protect against most of the members of the Tick-Borne Encephalitis complex including Kyasanur forest disease virus and Alkhumra virus, but that the neutralisation of Powassan virus following Tick-Borne Encephalitis vaccination is minimal.
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Tick Borne Encephalitis without cerebrospinal fluid pleocytosis.
BMC infectious diseases, 2014Co-Authors: Daša Stupica, Franc Strle, Tatjana Avšič-Županc, Mateja Logar, Blaž Pečavar, Fajko F. BajrovićAbstract:Background Tick Borne Encephalitis is the most frequent vector-transmitted infectious disease of the central nervous system in Europe and Asia. The disease caused by European subtype of Tick Borne Encephalitis virus has typically a biphasic clinical course with the second phase presenting as meningitis, meningoEncephalitis, or meningoencephalomyelitis. Cerebrospinal fluid pleocytosis is considered a condition sine qua non for the diagnosis of neurologic involvement in Tick Borne Encephalitis, which in routine clinical practice is confirmed by demonstration of serum IgM and IgG antibodies to Tick Borne Encephalitis virus.
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Acute myositis associated with the initial phase of Tick-Borne Encephalitis.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2011Co-Authors: Maja Arnež, Tatjana Avšič-Županc, Eva Ružić-sabljićAbstract:We present a case of clinically overt acute myositis associated with the initial phase of Tick-Borne Encephalitis in a ten-year-old boy. The diagnosis of the disease was confirmed by detection of Tick-Borne Encephalitis virus RNA in blood prior to the development of serum specific antibodies and the second phase of the disease.
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Tick-Borne Encephalitis in children: an update on epidemiology and diagnosis.
Expert review of anti-infective therapy, 2009Co-Authors: Maja Arnež, Tatjana Avšič-ŽupancAbstract:Tick-Borne Encephalitis is an infection of the CNS caused by a Tick-Borne Encephalitis virus transmitted by Ticks. It is more common in adults than in children. During the last 30 years, the incidence of the disease increased continuously in almost all endemic European countries except Austria. Many factors are responsible for the increased incidence. However, in Austria, the incidence of Tick-Borne Encephalitis decreased dramatically since the introduction of a well-organized vaccination campaign against Tick-Borne Encephalitis. The diagnosis of Tick-Borne Encephalitis is based on clinical criteria and laboratory confirmation of infection. Other Tick-Borne diseases, such as Lyme borreliosis and human granulocytic anaplasmosis, should be considered in children with Tick-Borne Encephalitis since endemic areas for all three diseases overlap.
Christian Kunz - One of the best experts on this subject based on the ideXlab platform.
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Tick-Borne Encephalitis in the golden agers: A conference report of the International Scientific Working Group on Tick-Borne Encephalitis (ISW TBE)
Vaccine, 2006Co-Authors: Ursula Kunze, Franz X. Heinz, Ulf Baumhackl, Rudolf Bretschneider, Vaclav Chmelik, Beatrix Grubeck-loebenstein, Mats Haglund, Reinhard Kaiser, P. Kimmig, Christian KunzAbstract:The international consensus "Tick-Borne Encephalitis in childhood" was an important and successful achievement of the International Scientific Working Group on Tick-Borne Encephalitis (ISW TBE) [1]. The main topic of the 2005 conference meeting was "Tick-Borne Encephalitis in the golden agers". "Golden agers" is the synonym for people over 50 years of age. Still the incidence of TBE is underestimated, even in countries where the disease is known and there must be European regions where TBE occurs and is not yet diagnosed properly. Increasing mobility and the changes in lifestyle of people during the last 10 to 15 years, also in the age group over 50 years, have exacerbated the risk and the number of infections. In order to get more information about Tick-Borne Encephalitis in older ages, data of seven European countries were presented (Austria, Croatia, Czech Republic, Germany, Lithuania, Poland and Sweden). Based on the scientific findings adequate conclusions were drafted by the ISW TBE.