The Experts below are selected from a list of 24 Experts worldwide ranked by ideXlab platform

Martin Jeffrey - One of the best experts on this subject based on the ideXlab platform.

  • Intracytoplasmic Tingible Body Macrophage (TBM)-associated immunolabelling.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    a. Scrapie-affected tonsil. PrPd immunogold labelling. Immunogold labelling is present at the membrane of the random endoplasmic reticulum (ER) network within the cytoplasm of a Tingible Body Macrophage (TBM). Immunogold labelling is also present within an adjacent lysosome (asterisk). Bar, 1 µm. b. Scrapie-affected tonsil. PrPd immunogold labelling. Immunogold labelling is associated with the membrane of random ER networks within the cytoplasm of a TBM. Adjacent to these PrPd labelled structures are areas of ER (arrows). Lysosomes are sparsely labelled (asterisk). Bar, 400 nm. c. (and insert). Scrapie-affected tonsil. PrPd immunogold labelling. PrPd immunolabelling is present on the plasma-membrane of a TBM. In addition, discreet vesicular structures contiguous with the plasma-membrane or within the cytoplasm of the TBM show immunogold labelling (arrowheads). Adjacent to these structures, a clathrin-coated pit is not labelled for PrPd (arrow). A random network of ER is also immunolabelled. A lysosome shows PrPd accumulation (asterisk). Bar, 1 µm. d. Scrapie-affected tonsil. Ubiquitin immunogold labelling. A random network of convoluted ER shows immunogold labelling of the membrane. Labelling is not present within the adjacent lysosomes (asterisks). Bar, 2 µm.

  • Immunolabelling of scrapie-affected tonsil using antibodies directed to the C' or N' terminus of PrP.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    Paraffin-embedded tonsil from clinically-affected sheep. Serial sections. PrPd immunolabelling. a. The follicle shows both widespread follicular dendritic cell (FDC) labelling interspersed between lymphocytes and multiple granules of intracytoplasmic Tingible Body Macrophage (TBM) labelling (arrows) using R145 antiBody. Bar, 130 µm. b. Using BG4 antiBody, light FDC labelling and single intracytoplasmic granules of TBM labelling are present (arrows). Bar, 130 µm.

  • PrPd immunolabelling of follicle and perifollicular area of scrapie-affected tonsil.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    Paraffin-embedded tonsil from clinically-affected sheep. PrPd immunolabelling. Diffuse follicular dendritic cell (FDC) and granular Tingible Body Macrophage (TBM) labelling are present within the follicle, additionally, granular labelling is associated with the peri-follicular area and the epithelium of the tonsil. Follicular dendritic cell labelling is present throughout the light zone of the follicle (bottom) while TBM labelling is conspicuous in the dark zone (top) Bar, 290 µm.

  • Diagram of the proposed mechanisms of PrPd transfer and internalisation.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    a. Follicular dendritic cell (FDC)/B cell interactions. PrPc is converted to PrPd at the plasmalemma of the FDC. The molecule is passively transferred to the B cell during immune complex stimulation (possibly due to complex linkage), where it remains on the plasmalemma. FDCs do not re-internalise PrPd. b. FDC/Tingible Body Macrophage (TBM) interactions. PrPd may also be transferred from FDCs to TBMs where it is internalised after interaction with a trans-membrane ligand via a ubiquitin-mediated endocytic pathway and resulting in the accumulation of PrPd at the membrane of the endoplasmic reticulum.

Gillian Mcgovern - One of the best experts on this subject based on the ideXlab platform.

  • Intracytoplasmic Tingible Body Macrophage (TBM)-associated immunolabelling.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    a. Scrapie-affected tonsil. PrPd immunogold labelling. Immunogold labelling is present at the membrane of the random endoplasmic reticulum (ER) network within the cytoplasm of a Tingible Body Macrophage (TBM). Immunogold labelling is also present within an adjacent lysosome (asterisk). Bar, 1 µm. b. Scrapie-affected tonsil. PrPd immunogold labelling. Immunogold labelling is associated with the membrane of random ER networks within the cytoplasm of a TBM. Adjacent to these PrPd labelled structures are areas of ER (arrows). Lysosomes are sparsely labelled (asterisk). Bar, 400 nm. c. (and insert). Scrapie-affected tonsil. PrPd immunogold labelling. PrPd immunolabelling is present on the plasma-membrane of a TBM. In addition, discreet vesicular structures contiguous with the plasma-membrane or within the cytoplasm of the TBM show immunogold labelling (arrowheads). Adjacent to these structures, a clathrin-coated pit is not labelled for PrPd (arrow). A random network of ER is also immunolabelled. A lysosome shows PrPd accumulation (asterisk). Bar, 1 µm. d. Scrapie-affected tonsil. Ubiquitin immunogold labelling. A random network of convoluted ER shows immunogold labelling of the membrane. Labelling is not present within the adjacent lysosomes (asterisks). Bar, 2 µm.

  • Immunolabelling of scrapie-affected tonsil using antibodies directed to the C' or N' terminus of PrP.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    Paraffin-embedded tonsil from clinically-affected sheep. Serial sections. PrPd immunolabelling. a. The follicle shows both widespread follicular dendritic cell (FDC) labelling interspersed between lymphocytes and multiple granules of intracytoplasmic Tingible Body Macrophage (TBM) labelling (arrows) using R145 antiBody. Bar, 130 µm. b. Using BG4 antiBody, light FDC labelling and single intracytoplasmic granules of TBM labelling are present (arrows). Bar, 130 µm.

  • PrPd immunolabelling of follicle and perifollicular area of scrapie-affected tonsil.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    Paraffin-embedded tonsil from clinically-affected sheep. PrPd immunolabelling. Diffuse follicular dendritic cell (FDC) and granular Tingible Body Macrophage (TBM) labelling are present within the follicle, additionally, granular labelling is associated with the peri-follicular area and the epithelium of the tonsil. Follicular dendritic cell labelling is present throughout the light zone of the follicle (bottom) while TBM labelling is conspicuous in the dark zone (top) Bar, 290 µm.

  • Diagram of the proposed mechanisms of PrPd transfer and internalisation.
    2013
    Co-Authors: Gillian Mcgovern, Martin Jeffrey
    Abstract:

    a. Follicular dendritic cell (FDC)/B cell interactions. PrPc is converted to PrPd at the plasmalemma of the FDC. The molecule is passively transferred to the B cell during immune complex stimulation (possibly due to complex linkage), where it remains on the plasmalemma. FDCs do not re-internalise PrPd. b. FDC/Tingible Body Macrophage (TBM) interactions. PrPd may also be transferred from FDCs to TBMs where it is internalised after interaction with a trans-membrane ligand via a ubiquitin-mediated endocytic pathway and resulting in the accumulation of PrPd at the membrane of the endoplasmic reticulum.

Andras K. Szakal - One of the best experts on this subject based on the ideXlab platform.

  • Kinetics of the Tingible Body Macrophage response in mouse germinal center development and its depression with age.
    The Anatomical record, 1991
    Co-Authors: John P. Smith, A. M. Lister, John G. Tew, Andras K. Szakal
    Abstract:

    Although Tingible Body Macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of Tingible bodies or histochemically by using the monoclonal antiBody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.

John P. Smith - One of the best experts on this subject based on the ideXlab platform.

  • Kinetics of the Tingible Body Macrophage response in mouse germinal center development and its depression with age.
    The Anatomical record, 1991
    Co-Authors: John P. Smith, A. M. Lister, John G. Tew, Andras K. Szakal
    Abstract:

    Although Tingible Body Macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of Tingible bodies or histochemically by using the monoclonal antiBody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.

A. M. Lister - One of the best experts on this subject based on the ideXlab platform.

  • Kinetics of the Tingible Body Macrophage response in mouse germinal center development and its depression with age.
    The Anatomical record, 1991
    Co-Authors: John P. Smith, A. M. Lister, John G. Tew, Andras K. Szakal
    Abstract:

    Although Tingible Body Macrophages (TBM) have been recognized in germinal centers for over 100 years, their role in the germinal center response is not clear. In this study, the kinetics of the TBM response was quantitatively assessed and correlated with the kinetics of germinal center development in young mice. The TBM response in old mice (which have an age-related depression of germinal center development; Szakal et al., 1990) was analyzed for comparison. Young and old immune mice were challenged with human serum albumin and 0, 1, 3, 5, 7, 10, and 14 days later the popliteal and axillary lymph nodes were evaluated. Germinal centers were localized histochemically in alternate serial sections using horseradish peroxidase conjugated peanut agglutinin. TBM numbers were determined per germinal center on adjacent sections by the presence of Tingible bodies or histochemically by using the monoclonal antiBody Mac-2. Analysis of lymph nodes from young mice showed that TBM numbers decreased with the dissociation of preexisting germinal centers. TBM reappeared 5 days after challenge and the TBM kinetics paralleled the increase in size of de novo germinal centers. In fact, a constant ratio of one TBM to every 350-450 B cells was maintained from day 5 to day 10. In old lymph nodes, TBM were generally absent throughout germinal center development. The lack of TBM prior to germinal center development and their absence in aged mice are inconsistent with the concept that TBM are required for the induction of the germinal center reaction. However, the data are consistent with a role for TBM in regulating the magnitude of the germinal center reaction.