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Ikhlas A. Khan - One of the best experts on this subject based on the ideXlab platform.
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Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements.
Planta Medica, 2020Co-Authors: Abidah Parveen, Vijayasankar Raman, John S. Adams, Jane Manfron Budel, Jianping Zhao, Ganesh N.m. Babu, Zulfiqar Ali, Ikhlas A. KhanAbstract:Overlapping geographical occurrence, history of traditional use, confusion in species identification, and morphological resemblances among various species are some considerations that necessitate the importance of qualitative analysis for efficient quality control and safer botanical products. This paper provides detailed morpho-anatomies of the leaves and stems of Tinospora cordifolia, Tinospora crispa, and Tinospora sinensis, and stems of Tinospora baenzigeri. Microscopy studies of the selected Tinospora species revealed key diagnostic features that can help distinguish the closely related species of Tinospora as well as to detect any adulteration or substitution in the raw materials. HPTLC profiles of the authenticated plant materials, as well as commercial products claiming to contain Tinospora, were compared to distinguish T. crispa from other closely related species and to establish an efficient method to assess the identity and quality of the products using qualified chemical markers. HPTLC chromatograms of both plant samples and dietary supplements were compared with six reference marker compounds. The analysis revealed that borapetoside B and C were useful to identify T. crispa while tinosineside A was found to be characteristic to authenticate the T. sinensis products.
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development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds
Journal of Pharmaceutical and Biomedical Analysis, 2020Co-Authors: Abidah Parveen, Omer Fantoukh, Manal Alhusban, Vijayasankar Raman, Yan-hong Wang, Ikhlas A. KhanAbstract:Abstract Tinospora species are morphologically similar. Several cases of human toxicity have been reported in association with T. crispa. A chemical fingerprint was developed to differentiate T. crispa from its closely related species and to quantitate its major furanoditerpenes namely as borapetosides B, C and F. The rapid, sensitive and repeatable method was established using ultra–high performance liquid chromatography coupled with photodiode array and single quadrupole electrospray mass spectrometry detectors using a flavonoid, two alkaloids, an amide and six diterpenoids. Qualitative and quantitative determination was performed by UHPLC-UV and confirmed by MS. The intra-day RSD for replicates was between 0.9 and 6.8% and inter-day RSD was between 1.2 and 9.1%. Recovery was 97–103 %. The method is useful to achieve decisiveness in not only identifying but also differentiating T. crispa from T. sinensis and other closely related Tinospora species. Seventeen Tinospora plant samples and seventeen dietary supplements claiming T. crispa, T. sinensis and T. cordifolia were analyzed. The newly developed and validated method successfully resulted in the conclusive identification of two dietary supplements to be mislabeled.
Peter G. Waterman - One of the best experts on this subject based on the ideXlab platform.
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Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth
Phytotherapy research : PTR, 2004Co-Authors: David N Leach, Stephen P Myers, Gregory J. Leach, G. David Lin, Donald J. Brushett, Peter G. WatermanAbstract:Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A (PA). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA with the IC values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids. Copyright
Badri Prasad Shaw - One of the best experts on this subject based on the ideXlab platform.
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preliminary studies on anti inflammatory activity of zingber officinale rosc vitex negundo linn and Tinospora cordifolia willid miers in albino rats
Indian Journal of Pharmacology, 1999Co-Authors: U Jana, Rabindra Nath Chattopadhyay, Badri Prasad ShawAbstract:Objectives: To study the anti-inflammatory effect of Zingiber officinale, Vitex negundo and Tinospora cordifolia on carrageenin induced hind paw oedema and cotton pellet granuloma in rats. Methods: Hind paw oedema was produced by subplanter injection of carrageenin and paw volume was measured plethysmometrically at '0' and '3' hours intervals after injection. Cotton pellet granuloma was produced by implantation of 50(1mg sterile cotton in each axilla under ether anaesthesia. The animals were treated with Zingiber officinale, Vitex negundo, Tinospora cordifolia and the standard drugs acetylsalicylic acid and phenylbutazone. Results: Zingiber officinale, Vitex negundo and Tinospora cordifolia produced significant anti-inflammatory effect in both acute and subacute models of inflammation. In acute inflammation, effect of Tinospora cordifolia was more than acetylsalicylic acid. In subacute inflammation, the results of these drugs were less than phenylbutazone. Conclusion: Zingiber officinale, Vitex negundo and Tinospora cordifolia possess anti-inflammatory effects in both acute and subacute inflammation.
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PRELIMINARY STUDIES ON ANTI – INFLAMMATORY ACTIVITY OF ZINGBER OFFICINALE ROSC, VITEX NEGUNDO LINN AND Tinospora CORDIFOLIA (WILLID) MIERS IN ALBINO RATS
Indian Journal of Pharmacology, 1999Co-Authors: U Jana, Rabindra Nath Chattopadhyay, Badri Prasad ShawAbstract:Objectives: To study the anti-inflammatory effect of Zingiber officinale, Vitex negundo and Tinospora cordifolia on carrageenin induced hind paw oedema and cotton pellet granuloma in rats. Methods: Hind paw oedema was produced by subplanter injection of carrageenin and paw volume was measured plethysmometrically at '0' and '3' hours intervals after injection. Cotton pellet granuloma was produced by implantation of 50(1mg sterile cotton in each axilla under ether anaesthesia. The animals were treated with Zingiber officinale, Vitex negundo, Tinospora cordifolia and the standard drugs acetylsalicylic acid and phenylbutazone. Results: Zingiber officinale, Vitex negundo and Tinospora cordifolia produced significant anti-inflammatory effect in both acute and subacute models of inflammation. In acute inflammation, effect of Tinospora cordifolia was more than acetylsalicylic acid. In subacute inflammation, the results of these drugs were less than phenylbutazone. Conclusion: Zingiber officinale, Vitex negundo and Tinospora cordifolia possess anti-inflammatory effects in both acute and subacute inflammation.
Abidah Parveen - One of the best experts on this subject based on the ideXlab platform.
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Comparative Morpho-Anatomical and HPTLC Profiling of Tinospora Species and Dietary Supplements.
Planta Medica, 2020Co-Authors: Abidah Parveen, Vijayasankar Raman, John S. Adams, Jane Manfron Budel, Jianping Zhao, Ganesh N.m. Babu, Zulfiqar Ali, Ikhlas A. KhanAbstract:Overlapping geographical occurrence, history of traditional use, confusion in species identification, and morphological resemblances among various species are some considerations that necessitate the importance of qualitative analysis for efficient quality control and safer botanical products. This paper provides detailed morpho-anatomies of the leaves and stems of Tinospora cordifolia, Tinospora crispa, and Tinospora sinensis, and stems of Tinospora baenzigeri. Microscopy studies of the selected Tinospora species revealed key diagnostic features that can help distinguish the closely related species of Tinospora as well as to detect any adulteration or substitution in the raw materials. HPTLC profiles of the authenticated plant materials, as well as commercial products claiming to contain Tinospora, were compared to distinguish T. crispa from other closely related species and to establish an efficient method to assess the identity and quality of the products using qualified chemical markers. HPTLC chromatograms of both plant samples and dietary supplements were compared with six reference marker compounds. The analysis revealed that borapetoside B and C were useful to identify T. crispa while tinosineside A was found to be characteristic to authenticate the T. sinensis products.
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development of a chemical fingerprint as a tool to distinguish closely related Tinospora species and quantitation of marker compounds
Journal of Pharmaceutical and Biomedical Analysis, 2020Co-Authors: Abidah Parveen, Omer Fantoukh, Manal Alhusban, Vijayasankar Raman, Yan-hong Wang, Ikhlas A. KhanAbstract:Abstract Tinospora species are morphologically similar. Several cases of human toxicity have been reported in association with T. crispa. A chemical fingerprint was developed to differentiate T. crispa from its closely related species and to quantitate its major furanoditerpenes namely as borapetosides B, C and F. The rapid, sensitive and repeatable method was established using ultra–high performance liquid chromatography coupled with photodiode array and single quadrupole electrospray mass spectrometry detectors using a flavonoid, two alkaloids, an amide and six diterpenoids. Qualitative and quantitative determination was performed by UHPLC-UV and confirmed by MS. The intra-day RSD for replicates was between 0.9 and 6.8% and inter-day RSD was between 1.2 and 9.1%. Recovery was 97–103 %. The method is useful to achieve decisiveness in not only identifying but also differentiating T. crispa from T. sinensis and other closely related Tinospora species. Seventeen Tinospora plant samples and seventeen dietary supplements claiming T. crispa, T. sinensis and T. cordifolia were analyzed. The newly developed and validated method successfully resulted in the conclusive identification of two dietary supplements to be mislabeled.
David N Leach - One of the best experts on this subject based on the ideXlab platform.
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Anti-inflammatory Activity, Cytotoxicity and Active Compounds of Tinospora smilacina Benth
Phytotherapy research : PTR, 2004Co-Authors: David N Leach, Stephen P Myers, Gregory J. Leach, G. David Lin, Donald J. Brushett, Peter G. WatermanAbstract:Tinospora smilacina Benth. has been used in Australian indigenous medicine for the treatment of headache, rheumatoid arthritis and other inflammatory disorders. As part of an investigation into the anti-inflammatory potential of plants using an ethnopharmacological approach, the present study sought to evaluate the efficacy and safety of Tinospora smilacina. An ethanol extract of this plant was evaluated in vitro for anti-inflammatory activities on cyclooxygenase-1 (COX-1), cyclooxygenase-2 (COX-2), 5-lipoxygenase (5-LO) and phospholipase A (PA). The ethanol extract of Tinospora smilacina showed inhibitory activities on COX-1, COX-2, 5-LO and PA with the IC values of 63.5, 81.2, 92.1 and 30.5 μg/mL respectively. Cytotoxic effect of the extracts of Tinospora smilacina was investigated in vitro using ATP-based luminescence assay and the results showed no cytotoxic effect on cell lines of skin fibroblasts (1BR3), human Caucasian hepatocyte carcinoma (Hep G2) and human Caucasian promyelocytic leukaemia (HL-60). This paper also describes the results of fractionations and bioassay guided chemical studies, suggesting that the anti- inflammatory activity is due to triterpene-fatty acid esters and free fatty acids. Copyright
