The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Tetsushi Taguchi - One of the best experts on this subject based on the ideXlab platform.
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enhanced Tissue penetration induced high bonding strength of a novel Tissue Adhesive composed of cholesteryl group modified gelatin and disuccinimidyl tartarate
Colloids and Surfaces B: Biointerfaces, 2012Co-Authors: Tetsushi Taguchi, Miyuki Matsuda, Masami Ueno, Yoshiaki Endo, Motoki Inoue, Makoto SasakiAbstract:The aim of this study was to evaluate the effect of cholesteryl group content on the bonding strength of a novel Tissue Adhesive composed of cholesteryl group-modified geletin (CholGltn) and disuccinimidyl tartarate (DST). The bonding strength of this Tissue Adhesive with fresh arterial media reached a maximum at a CholGltn content of 70% in the CholGltn/gelatin (Gltn) mixture, which then decreased with increasing CholGltn content with a fixed succinimidyl group:amino group ratio of 1:1. The maximum bonding strength obtained was 6-fold higher compared with that of the original Gltn. Furthermore, maximum peeling strength was also obtained at a CholGltn content of 70% in the CholGltn/Gltn mixture and at a similar succinimidyl group:amino group ratio. The highest peeling strength was 8-fold higher compared with Gltn and 6-fold higher compared with commercial aldehyde-based Adhesive. After exposure of FITC-labeled Gltn or CholGltn to aortic smooth muscle cells (SMCs), which are abundant in arterial media, CholGltn integrated effectively with the surface of SMCs. This indicated that FITC-labeled CholGltn anchors into the cell membrane of SMCs. From these results, it was demonstrated that Tissue Adhesive composed of a CholGltn/Gltn mixture and DST showed improved penetration into arterial media compared with Adhesive composed of Gltn and DST. This behavior supports the suggestion that the hydrophobic cholesteryl group in Gltn contributes to the enhanced bonding/peeling strength. This novel Tissue Adhesive may become a useful material in the clinical field for the treatment of aortic dissection.
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Antitumor effect of an injectable in-situ forming drug delivery system composed of a novel Tissue Adhesive containing doxorubicin hydrochloride.
European Journal of Pharmaceutics and Biopharmaceutics, 2007Co-Authors: Sachiro Kakinoki, Tetsushi TaguchiAbstract:Abstract Our group has developed a novel Tissue Adhesive composed of biomacromolecules and organic acid derivatives which have good biocompatibility and exhibit high bonding strength to living Tissues. We propose to use this Tissue Adhesive for in-situ forming drug delivery system (DDS) for cancer chemotherapy. In a previous work, we had prepared a novel in-situ forming DDS composed of human serum albumin (HSA) and tartaric acid derivative (TAD) containing doxorubicin hydrochloride (DOX), and we had demonstrated an in vitro release profile of DOX from HSA–TAD gel for approximately up to 100 h. Here, we report on antitumor effect of this injectable in-situ forming DDS. Local injection of DOX by the HSA–TAD was administered to human colon carcinoma (WiDr) implanted subcutaneously onto the immunodeficient mouse. The results of the in vivo experiments showed that the presence of DOX in blood of mice was detectable for up to 3 days, and that the tumor volume was effectively minimized with injection of HSA–TAD containing DOX. The in-situ forming DDS with the novel Tissue Adhesive containing DOX, therefore, is a useful technique for cancer chemotherapy.
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Injectable in situ forming drug delivery system for cancer chemotherapy using a novel Tissue Adhesive: characterization and in vitro evaluation.
European Journal of Pharmaceutics and Biopharmaceutics, 2006Co-Authors: Sachiro Kakinoki, Tetsushi Taguchi, Hirofumi Saito, Junzo Tanaka, Tetsuya TateishiAbstract:Abstract Injectable polymers that are biocompatible and biodegradable are important biomaterials for drug delivery system (DDS) and Tissue engineering. We have already developed novel Tissue Adhesives consisting of biomacromolecules and organic acid derivatives with active ester groups. The resulting Tissue Adhesive forms in situ as a gel and has high bonding strength for living Tissue as well as it has good biocompatibility and biodegradability. Here, we report on the physicochemical properties and in vitro evaluation of this novel Tissue Adhesive consisting of human serum albumin (HSA) and tartaric acid derivative (TAD) containing doxorubicin hydrochloride (DOX). The results of the measurement of physicochemical characteristics indicate that the gelation time and gel strength of HSA–TAD gels can be controlled according to the material composition. The bonding strength of HSA–TAD Adhesives was found to be sufficient to adhere at focus and to correspond with the cross-linking density of HSA–TAD gels. Furthermore, the release of DOX from HSA–TAD gels was sustained for approximately 100 h in an in vitro evaluation. The novel Tissue Adhesive, therefore, is expected to be applicable for use as an injectable in situ forming DDS.
James Quinn - One of the best experts on this subject based on the ideXlab platform.
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a new Tissue Adhesive for laceration repair in children
The Journal of Pediatrics, 1998Co-Authors: Thomas B Bruns, Bryan S Robinson, Ralph J Smith, Dale L Kile, Timothy P Davis, Kevin M Sullivan, James QuinnAbstract:To determine the effectiveness of a new Tissue Adhesive, 2-Octylcyanoacrylate (2-OCA), for laceration repair, 83 children presenting to T.C. Thompson Children's Hospital Emergency Department with lacerations meeting eligibility requirements between February and June 1996 were randomized to receive 2-OCA or nonabsorbable sutures/staples. The length of time for repair was recorded. The length of time for laceration repair was decreased (2.9 minutes 2-OCA vs 5.8 minutes suture/staple; p < 0.001), the parents' assessment of the pain felt by their children in the 2-OCA group was less, and the wounds closed with Tissue Adhesive had slightly lower cosmesis scores. 2-OCA is an acceptable alternative to conventional methods of wound repair with comparable cosmetic outcome.
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octylcyanoacrylate Tissue Adhesive versus suture wound repair in a contaminated wound model
Surgery, 1997Co-Authors: James Quinn, Jennifer Maw, Karam Ramotar, Georg Wenckebach, George WellsAbstract:Abstract Background. Octylcyanoacrylate Tissue Adhesive is a topical wound closure that precludes the need for foreign bodies (sutures) to close wounds. It also has an in vitro antimicrobial effect when standard disc sensitivity tests are used. Methods. To determine whether contaminated wounds closed with octylcyanoacrylate Tissue Adhesive will have a lower infection rate compared with wounds dosed with 5-0 monofilament sutures, we designed a randomized, blinded, experimental animal study. Two incisions were made on 20 albino guinea pigs. The wounds were contaminated with 105 Staphylococcus aureus ATCC 12600 and randomly assigned to be closed with either topical octylcyanoacrylate Tissue Adhesive or percutaneous 5-0 polypropylene suture. Five days later the Adhesive and sutures were removed, and a section of the wound was given to a histopathologist blinded to the type of wound closure. The wound was determined to be infected if inflammatory cells with intracellular cocci were seen. The rest of the wound was opened and examined for clinical evidence of infection. Quantitative bacteriologic analysis was performed. Results. Five wounds in the Tissue Adhesive group were sterile on day 5, whereas all sutured wounds had positive cultures (25% versus 0%, p Conclusions. Contaminated wounds closed with sutures had higher infection rates compared with those repaired with topical Tissue Adhesive. The amount of colonization may not be an accurate method to determine infection.
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a randomized trial comparing octylcyanoacrylate Tissue Adhesive and sutures in the management of lacerations
JAMA, 1997Co-Authors: James Quinn, Jennifer Maw, George Wells, Terri Sutcliffe, Mario Jarmuske, Ian G Stiell, Peter JohnsAbstract:Objective. —To assess the effectiveness of a new Tissue Adhesive for laceration closure. Design. —A prospective, randomized controlled trial. Setting. —An adult teaching hospital. Participants. —One hundred thirty patients with 136 lacerations who consented to enrollment during a 5-month period. The lacerations included all eligible nonmucosal facial lacerations, as well as selected extremity and torso lacerations (not on hands, feet, or joints). One hundred six lacerations were available for early follow-up, and 98 were available for 3-month evaluation. Interventions. —Lacerations were randomly allocated to have skin closure with octylcyanoacrylate Adhesive or monofilament suture. Main Outcome Measure. —A 3-month photograph of the wound was assigned a cosmesis score on a previously validated 100-mm visual analog cosmesis scale by a plastic surgeon who was unaware of the method of wound closure. Results. —There were no differences in the mean visual analog cosmesis scores (67 mm for octylcyanoacrylate vs 68 mm for sutures; P =.65). Similarly, there was no difference in the percentage of early (80% vs 82%; P =.80) or late (72% vs 75%; P =.74) optimal wound evaluation scores. The Tissue Adhesive was a faster method of wound repair (3.6 vs 12.4 minutes; P P Conclusions. —Octylcyanoacrylate Tissue Adhesive effectively closes selected lacerations. This relatively painless and fast method of wound repair can replace the need for suturing several million lacerations each year.
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economic comparison of a Tissue Adhesive and suturing in the repair of pediatric facial lacerations
The Journal of Pediatrics, 1995Co-Authors: Martin H Osmond, Terry P Klassen, James QuinnAbstract:Abstract Objective: To determine, from the societal perspective, the most cost efficient of the three methods commonly used to repair pediatric facial lacerations: nondissolving sutures, dissolving sutures, or a Tissue Adhesive (Histoacryl blue). Design: Cost-minimization analysis and willingness-to-pay survey. Setting: Tertiary-care pediatric emergency department. Methods: All differential costs relevant to equipment utilization, pharmaceutical use, health care worker time, and parental loss of income for follow-up visits were calculated for each method. On the basis of previous research, our model assumes equal cosmetic outcome for the three methods. In addition, a convenience sample of 30 parents were surveyed in the emergency department to rank their preferences and willingness to pay for the three methods of wound closure. Results: The reduction in cost (in Canadian dollars) per patient of switching from the standard nondissolving sutures was $49.60 for switching to Tissue Adhesive and $37.90 for dissolving sutures. Sensitivity analyses performed on key variables did not significantly alter our conclusions. Of those parents surveyed, 90% (95% confidence interval, 74% to 98%) chose Tissue Adhesive and 10% (95% confidence interval, 2% to 26%) chose dissolving sutures as their first choice for wound closure. Nondissolving sutures were ranked third by 29 of 30 parents. Parents were willing to pay a median (25th to 75th percentile) of $40 ($25 to $100) for Tissue Adhesive and $25 ($10 to $56) for dissolving sutures if only nondissolving sutures were provided by the health care system ( p = 0.1). Conclusions: Tissue Adhesive is the preferred method of closure of pediatric facial lacerations because it results in the most efficient use of resources and is preferred by the majority of parents. (J PEDIATR 1995;126:892-5)
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Tissue Adhesive wound repair revisited
The Journal of emergency medicine, 1994Co-Authors: J. Pieter Noordzij, Pamela A. Foresman, George T. Rodeheaver, James Quinn, Richard F. EdlichAbstract:The purpose of this experimental study was to compare the effect of a Tissue Adhesive, N-butyl-2-cyanoacrylate, on the wound's ability to resist infection and gain strength to the effect of percutaneous polypropylene suture. Percutaneous sutures damaged host defenses, inviting the growth of bacteria to a level that was significantly greater than that encountered with the Tissue Adhesive. Immediately after wound closure, percutaneous sutures provided a more secure closure, as measured by breaking strength, than did Tissue Adhesives. Seven days later, the breaking strengths of wounds closed by Tissue Adhesives did not differ significantly from those repaired with percutaneous sutures. Tissue Adhesive closure requires less psychomotor skills than suture closure and is accomplished more rapidly than suture closure.
Hiromi Kurosawa - One of the best experts on this subject based on the ideXlab platform.
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a novel synthetic Tissue Adhesive hydrogel using a crosslinkable polymeric micelle
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Yoshihiko Murakami, Masayuki Yokoyama, Teruo Okano, H Nishida, Yasuko Tomizawa, Masahiro Endo, Hiromi KurosawaAbstract:We prepared a novel Tissue-Adhesive hydrogel by using a polymeric micelle consisting of an aldehyde-terminated poly(ethylene glycol)–poly(D,L-lactide) (PEG–PLA) block polymer. A Schiff base is chemically formed between the amino groups in a polyallylamine and the aldehyde groups on the surface of polymeric micelles. The hydrogel was formed in ∼2 s when the polymeric micelle solution and polyallylamine solution are mixed in vitro. The hydrogel was rapidly formed in vivo, and it adhered to a Tissue surface. Our novel Tissue-Adhesive hydrogel creates no risk of infectious contaminations, because it consists of only synthetic materials. Further, PEG and PLA are known to be biocompatible and noncytotoxic. The results obtained in the present study show that a hydrogel prepared by the formation of a Schiff base between aldehyde and amine groups will potentially address the need for novel Tissue-Adhesive materials. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
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a novel synthetic Tissue Adhesive hydrogel using a crosslinkable polymeric micelle
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Yoshihiko Murakami, Masayuki Yokoyama, Teruo Okano, H Nishida, Yasuko Tomizawa, Masahiro Endo, Hiromi KurosawaAbstract:We prepared a novel Tissue-Adhesive hydrogel by using a polymeric micelle consisting of an aldehyde-terminated poly(ethylene glycol)–poly(D,L-lactide) (PEG–PLA) block polymer. A Schiff base is chemically formed between the amino groups in a polyallylamine and the aldehyde groups on the surface of polymeric micelles. The hydrogel was formed in ∼2 s when the polymeric micelle solution and polyallylamine solution are mixed in vitro. The hydrogel was rapidly formed in vivo, and it adhered to a Tissue surface. Our novel Tissue-Adhesive hydrogel creates no risk of infectious contaminations, because it consists of only synthetic materials. Further, PEG and PLA are known to be biocompatible and noncytotoxic. The results obtained in the present study show that a hydrogel prepared by the formation of a Schiff base between aldehyde and amine groups will potentially address the need for novel Tissue-Adhesive materials. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
Sachiro Kakinoki - One of the best experts on this subject based on the ideXlab platform.
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Antitumor effect of an injectable in-situ forming drug delivery system composed of a novel Tissue Adhesive containing doxorubicin hydrochloride.
European Journal of Pharmaceutics and Biopharmaceutics, 2007Co-Authors: Sachiro Kakinoki, Tetsushi TaguchiAbstract:Abstract Our group has developed a novel Tissue Adhesive composed of biomacromolecules and organic acid derivatives which have good biocompatibility and exhibit high bonding strength to living Tissues. We propose to use this Tissue Adhesive for in-situ forming drug delivery system (DDS) for cancer chemotherapy. In a previous work, we had prepared a novel in-situ forming DDS composed of human serum albumin (HSA) and tartaric acid derivative (TAD) containing doxorubicin hydrochloride (DOX), and we had demonstrated an in vitro release profile of DOX from HSA–TAD gel for approximately up to 100 h. Here, we report on antitumor effect of this injectable in-situ forming DDS. Local injection of DOX by the HSA–TAD was administered to human colon carcinoma (WiDr) implanted subcutaneously onto the immunodeficient mouse. The results of the in vivo experiments showed that the presence of DOX in blood of mice was detectable for up to 3 days, and that the tumor volume was effectively minimized with injection of HSA–TAD containing DOX. The in-situ forming DDS with the novel Tissue Adhesive containing DOX, therefore, is a useful technique for cancer chemotherapy.
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Injectable in situ forming drug delivery system for cancer chemotherapy using a novel Tissue Adhesive: characterization and in vitro evaluation.
European Journal of Pharmaceutics and Biopharmaceutics, 2006Co-Authors: Sachiro Kakinoki, Tetsushi Taguchi, Hirofumi Saito, Junzo Tanaka, Tetsuya TateishiAbstract:Abstract Injectable polymers that are biocompatible and biodegradable are important biomaterials for drug delivery system (DDS) and Tissue engineering. We have already developed novel Tissue Adhesives consisting of biomacromolecules and organic acid derivatives with active ester groups. The resulting Tissue Adhesive forms in situ as a gel and has high bonding strength for living Tissue as well as it has good biocompatibility and biodegradability. Here, we report on the physicochemical properties and in vitro evaluation of this novel Tissue Adhesive consisting of human serum albumin (HSA) and tartaric acid derivative (TAD) containing doxorubicin hydrochloride (DOX). The results of the measurement of physicochemical characteristics indicate that the gelation time and gel strength of HSA–TAD gels can be controlled according to the material composition. The bonding strength of HSA–TAD Adhesives was found to be sufficient to adhere at focus and to correspond with the cross-linking density of HSA–TAD gels. Furthermore, the release of DOX from HSA–TAD gels was sustained for approximately 100 h in an in vitro evaluation. The novel Tissue Adhesive, therefore, is expected to be applicable for use as an injectable in situ forming DDS.
Yoshihiko Murakami - One of the best experts on this subject based on the ideXlab platform.
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a novel synthetic Tissue Adhesive hydrogel using a crosslinkable polymeric micelle
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Yoshihiko Murakami, Masayuki Yokoyama, Teruo Okano, H Nishida, Yasuko Tomizawa, Masahiro Endo, Hiromi KurosawaAbstract:We prepared a novel Tissue-Adhesive hydrogel by using a polymeric micelle consisting of an aldehyde-terminated poly(ethylene glycol)–poly(D,L-lactide) (PEG–PLA) block polymer. A Schiff base is chemically formed between the amino groups in a polyallylamine and the aldehyde groups on the surface of polymeric micelles. The hydrogel was formed in ∼2 s when the polymeric micelle solution and polyallylamine solution are mixed in vitro. The hydrogel was rapidly formed in vivo, and it adhered to a Tissue surface. Our novel Tissue-Adhesive hydrogel creates no risk of infectious contaminations, because it consists of only synthetic materials. Further, PEG and PLA are known to be biocompatible and noncytotoxic. The results obtained in the present study show that a hydrogel prepared by the formation of a Schiff base between aldehyde and amine groups will potentially address the need for novel Tissue-Adhesive materials. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
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a novel synthetic Tissue Adhesive hydrogel using a crosslinkable polymeric micelle
Journal of Biomedical Materials Research Part A, 2007Co-Authors: Yoshihiko Murakami, Masayuki Yokoyama, Teruo Okano, H Nishida, Yasuko Tomizawa, Masahiro Endo, Hiromi KurosawaAbstract:We prepared a novel Tissue-Adhesive hydrogel by using a polymeric micelle consisting of an aldehyde-terminated poly(ethylene glycol)–poly(D,L-lactide) (PEG–PLA) block polymer. A Schiff base is chemically formed between the amino groups in a polyallylamine and the aldehyde groups on the surface of polymeric micelles. The hydrogel was formed in ∼2 s when the polymeric micelle solution and polyallylamine solution are mixed in vitro. The hydrogel was rapidly formed in vivo, and it adhered to a Tissue surface. Our novel Tissue-Adhesive hydrogel creates no risk of infectious contaminations, because it consists of only synthetic materials. Further, PEG and PLA are known to be biocompatible and noncytotoxic. The results obtained in the present study show that a hydrogel prepared by the formation of a Schiff base between aldehyde and amine groups will potentially address the need for novel Tissue-Adhesive materials. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2007
