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Charles E Butler - One of the best experts on this subject based on the ideXlab platform.

  • decellularized skin adipose Tissue Flap matrix for engineering vascularized composite soft Tissue Flaps
    Acta Biomaterialia, 2016
    Co-Authors: Qixu Zhang, Joshua A Johnson, Lina W Dunne, Youbai Chen, Tejaswi Iyyanki, Edward I Chang, Cynthia D Branchbrooks, Geoffrey L Robb, Charles E Butler
    Abstract:

    Abstract Using a perfusion decellularization protocol, we developed a decellularized skin/adipose Tissue Flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro . We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo , the engineered Flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose Tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft Tissue Flap engineering. This platform is not limited by the Flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques. Statement of Significance Significant soft Tissue loss resulting from traumatic injury or tumor resection often requires surgical reconstruction using autologous soft Tissue Flaps. However, the limited availability of qualitative autologous Flaps as well as the donor site morbidity significantly limits this approach. Engineered soft Tissue Flap grafts may offer a clinically relevant alternative to the autologous Flap Tissue. In this study, we engineered vascularized soft Tissue free Flap by using skin/adipose Flap extracellular matrix scaffold (DSAF) in combination with multiple types of human cells. Following vascular reanastomosis in the recipient site, the engineered products successful regenerated large-scale fat Tissue in vivo . This approach may provide a translatable platform for composite soft Tissue free Flap engineering for microsurgical reconstruction.

  • Decellularized skin/adipose Tissue Flap matrix for engineering vascularized composite soft Tissue Flaps ☆
    Acta biomaterialia, 2016
    Co-Authors: Qixu Zhang, Joshua A Johnson, Lina W Dunne, Youbai Chen, Tejaswi Iyyanki, Edward I Chang, Geoffrey L Robb, Cynthia D. Branch-brooks, Charles E Butler
    Abstract:

    Abstract Using a perfusion decellularization protocol, we developed a decellularized skin/adipose Tissue Flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro . We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo , the engineered Flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose Tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft Tissue Flap engineering. This platform is not limited by the Flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques. Statement of Significance Significant soft Tissue loss resulting from traumatic injury or tumor resection often requires surgical reconstruction using autologous soft Tissue Flaps. However, the limited availability of qualitative autologous Flaps as well as the donor site morbidity significantly limits this approach. Engineered soft Tissue Flap grafts may offer a clinically relevant alternative to the autologous Flap Tissue. In this study, we engineered vascularized soft Tissue free Flap by using skin/adipose Flap extracellular matrix scaffold (DSAF) in combination with multiple types of human cells. Following vascular reanastomosis in the recipient site, the engineered products successful regenerated large-scale fat Tissue in vivo . This approach may provide a translatable platform for composite soft Tissue free Flap engineering for microsurgical reconstruction.

Shi Wen-jun - One of the best experts on this subject based on the ideXlab platform.

  • Clinical application of tracheal reconstruction by using pulmonary Tissue Flap with alloy stent
    China Journal of Modern Medicine, 2011
    Co-Authors: Shi Wen-jun
    Abstract:

    【Objective】 To determine the clinical value of pulmonary Tissue Flap with alloy stent in the reconstruction of the thoracic trachea.【Methods】 5 patients were treated by the following operation: the lobe bronchia were ligated and the gas in the lung was ventinged to create pulmonary Tissue Flap.Then alloy mesh stent was placed in the pulmonary Tissue Flap.The compound artificial substitution was used to repair the thoracic tracheal defect.These patients included: one case of right common tracheal scarred stenosis,one case of mixed cancerization in the lower part of the trachea,one case of tracheal tumor in the upper part and two cases of left common tracheal carcinoid.【Results】 Dyspnea in all patients relieved rapidly after the operation.No stenosis and granulation Tissue were observed in the prosthetic lumen.Four patients were good in follow-up,but one died for great hemoptysis after 14 months.【Conclusion】 Using autogeneic pulmonary Tissue Flap with alloy stent sometimes may be a choice to reconstruct the thoracic defect.

  • Clinical application of thoracic tracheal reconstruction by using pulmonary Tissue Flap combined with nickel-titanium alloy stent
    Shandong Medical Journal, 2008
    Co-Authors: Shi Wen-jun
    Abstract:

    Objective To study the usability of pulmonary Tissue Flap in the reconstruction of the thoracic trachea.MethodsClinically,4 patients were treated by the following operation:the nickel-titanium alloy mesh stent was placed inside the lumen to repair the defective tracheal wall by the pulmonary Tissue Flap with the vascularized segment.These patients included one case of right common tracheal scarred stenosis and artesia,one case of mixed cancerization in the lower part of the common trachea and two cases of left common tracheal carcinoid.ResultsNo stenosis and granulation Tissue were observed in the prosthetic lumen.All patients recovered respiratory function,and the effect was stable in follow-up.ConclusionThe pulmonary Tissue Flap is a feasible prosthesis of thoracic tracheal reconstruction.

Wen-jun Shi - One of the best experts on this subject based on the ideXlab platform.

  • Lung Tissue Flap repairs esophagus defection with an inner chitosan tube stent.
    World journal of gastroenterology, 2009
    Co-Authors: Gang Chen, Wen-jun Shi
    Abstract:

    AIM: To repair the partial esophagus defect with a chitosan stent, a new esophageal prosthesis made of pulmonary Tissue with vascular pedicle. METHODS: Fifteen Japanese big ear white rabbits were divided into experimental group (n = 10) and control group (n = 5). Esophagus defect in rabbits of experimental group was repaired using lung Tissue Flap with a chitosan tube stent, gross and histological appearance was observed at week 2, 4 and 8 after operation, and barium sulphate X-ray screen was performed at week 10 after operation. Esophagus defect of rabbits in control group was repaired using lung Tissue Flap with no chitosan tube stent, gross and histological appearance was observed at week 2, 4 and 8 after operation, and barium sulphate X-ray screen was performed at week 10 after operation. RESULTS: In the experimental group, 6 rabbits survived for over two weeks, the lung Tissue Flap healed esophageal defection, and squamous metaplasia occurred on the surface of lung Tissue Flap. At week 10 after operation, barium sulphate examination found that barium was fluent through the esophagus with no stricture or back stream, the creeping was good. In the control group, 4 rabbits survived for two weeks, the lung Tissue Flap healed esophageal defection with fibrous Tissue hyperplasia, barium sulphate examination found that barium was fluent through the esophagus with a slight stricture or back stream, and the creeping was not good at week 10 after operation. CONCLUSION: Esophagus defect can be repaired using lung Tissue Flap with an inner chitosan tube stent.

  • Clinical application and animal experiment of thoracic tracheal reconstruction by using pulmonary Tissue Flap
    Zhonghua wai ke za zhi [Chinese journal of surgery], 2003
    Co-Authors: Wen-jun Shi, Suning Zhang, Wei Yang, Jun-gang Zhao, Yang Zhao, Jun Liu
    Abstract:

    Objective To study the usability of pulmonary Tissue Flap in the reconstruction of the thoracic trachea. Methods Over half perimeter anterior and posterior wall and 6 to 8 tracheal cartilagious rings of dog trachea were resected. The nickel-titanium alloy mesh stent was placed inside the lumen for repair of the defect of the tracheal wall by nearby pulmonary Tissue Flap with the vascularized segment. The dogs were killed from 2 months to 12 months after operation. Specimens were taken and observed under light and electron microscope. Clinically, 4 patients were treated by this way operation [right common tracheal scarred stenosis and atresia (1), mixed cancerization in the lower part of the common trachea (1) and left common tracheal carcinoid (2)]. Results No stenosis and granulation Tissue were observed in the prosthetic lumen, in which there was comparative continuous stratified ciliated columnar epithelium. All patients recovered respiratory function. Conclusion Pulmonary Tissue Flap is a promising prosthesis of thoracic tracheal reconstruction.

Qixu Zhang - One of the best experts on this subject based on the ideXlab platform.

  • decellularized skin adipose Tissue Flap matrix for engineering vascularized composite soft Tissue Flaps
    Acta Biomaterialia, 2016
    Co-Authors: Qixu Zhang, Joshua A Johnson, Lina W Dunne, Youbai Chen, Tejaswi Iyyanki, Edward I Chang, Cynthia D Branchbrooks, Geoffrey L Robb, Charles E Butler
    Abstract:

    Abstract Using a perfusion decellularization protocol, we developed a decellularized skin/adipose Tissue Flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro . We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo , the engineered Flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose Tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft Tissue Flap engineering. This platform is not limited by the Flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques. Statement of Significance Significant soft Tissue loss resulting from traumatic injury or tumor resection often requires surgical reconstruction using autologous soft Tissue Flaps. However, the limited availability of qualitative autologous Flaps as well as the donor site morbidity significantly limits this approach. Engineered soft Tissue Flap grafts may offer a clinically relevant alternative to the autologous Flap Tissue. In this study, we engineered vascularized soft Tissue free Flap by using skin/adipose Flap extracellular matrix scaffold (DSAF) in combination with multiple types of human cells. Following vascular reanastomosis in the recipient site, the engineered products successful regenerated large-scale fat Tissue in vivo . This approach may provide a translatable platform for composite soft Tissue free Flap engineering for microsurgical reconstruction.

  • Decellularized skin/adipose Tissue Flap matrix for engineering vascularized composite soft Tissue Flaps ☆
    Acta biomaterialia, 2016
    Co-Authors: Qixu Zhang, Joshua A Johnson, Lina W Dunne, Youbai Chen, Tejaswi Iyyanki, Edward I Chang, Geoffrey L Robb, Cynthia D. Branch-brooks, Charles E Butler
    Abstract:

    Abstract Using a perfusion decellularization protocol, we developed a decellularized skin/adipose Tissue Flap (DSAF) comprising extracellular matrix (ECM) and intact vasculature. Our DSAF had a dominant vascular pedicle, microcirculatory vascularity, and a sensory nerve network and retained three-dimensional (3D) nanofibrous structures well. DSAF, which was composed of collagen and laminin with well-preserved growth factors (e.g., vascular endothelial growth factor, basic fibroblast growth factor), was successfully repopulated with human adipose-derived stem cells (hASCs) and human umbilical vein endothelial cells (HUVECs), which integrated with DSAF and formed 3D aggregates and vessel-like structures in vitro . We used microsurgery techniques to re-anastomose the recellularized DSAF into nude rats. In vivo , the engineered Flap construct underwent neovascularization and constructive remodeling, which was characterized by the predominant infiltration of M2 macrophages and significant adipose Tissue formation at 3 months postoperatively. Our results indicate that DSAF co-cultured with hASCs and HUVECs is a promising platform for vascularized soft Tissue Flap engineering. This platform is not limited by the Flap size, as the entire construct can be immediately perfused by the recellularized vascular network following simple re-integration into the host using conventional microsurgical techniques. Statement of Significance Significant soft Tissue loss resulting from traumatic injury or tumor resection often requires surgical reconstruction using autologous soft Tissue Flaps. However, the limited availability of qualitative autologous Flaps as well as the donor site morbidity significantly limits this approach. Engineered soft Tissue Flap grafts may offer a clinically relevant alternative to the autologous Flap Tissue. In this study, we engineered vascularized soft Tissue free Flap by using skin/adipose Flap extracellular matrix scaffold (DSAF) in combination with multiple types of human cells. Following vascular reanastomosis in the recipient site, the engineered products successful regenerated large-scale fat Tissue in vivo . This approach may provide a translatable platform for composite soft Tissue free Flap engineering for microsurgical reconstruction.

Chen Gang - One of the best experts on this subject based on the ideXlab platform.

  • Pedicled Tissue Flaps for repairing osteomyelitis and Tissue defect of the upper two-thirds of the tibia
    Journal of Xinjiang Medical University, 2004
    Co-Authors: Chen Gang
    Abstract:

    Objective: To study the clinic effect of the pedicle Tissue Flap repairing the upper tibia osteomyelitis with Tissue defect. Method: From 1998 to 2003, 24 patient with upper tibia osteomyelitis complicating Tissue defect were studied according to the varied Tissue defects and its injuried degree, 3 types of pedicle Tissue Flaps were designed and introduced. Results: All Flaps survived, 21 case were primary healing. 3 case delayed healing, the complication included local hematoma in 2 cases, wound surface was not healed and dead bone occurred in the local region in one case, af ter sequestrectomy the wound healed. Conclusion: Pedicle Tissue Flap Is a an easy, safe and reliable method for repairing the upper tibia osteomyelitis with Tissue defect.