The Experts below are selected from a list of 276 Experts worldwide ranked by ideXlab platform
Chulkyu Park - One of the best experts on this subject based on the ideXlab platform.
-
Ion Channels Involved in Tooth Pain.
International Journal of Molecular Sciences, 2019Co-Authors: Kihwan Lee, Chulkyu Park, Byeong-min Lee, Yong Ho Kim, Gehoon ChungAbstract:The Tooth has an unusual sensory system that converts external stimuli predominantly into Pain, yet its sensory afferents in teeth demonstrate cytochemical properties of non-nociceptive neurons. This review summarizes the recent knowledge underlying this paradoxical nociception, with a focus on the ion channels involved in Tooth Pain. The expression of temperature-sensitive ion channels has been extensively investigated because thermal stimulation often evokes Tooth Pain. However, temperature-sensitive ion channels cannot explain the sudden intense Tooth Pain evoked by innocuous temperatures or light air puffs, leading to the hydrodynamic theory emphasizing the microfluidic movement within the dentinal tubules for detection by mechanosensitive ion channels. Several mechanosensitive ion channels expressed in dental sensory systems have been suggested as key players in the hydrodynamic theory, and TRPM7, which is abundant in the odontoblasts, and recently discovered PIEZO receptors are promising candidates. Several ligand-gated ion channels and voltage-gated ion channels expressed in dental primary afferent neurons have been discussed in relation to their potential contribution to Tooth Pain. In addition, in recent years, there has been growing interest in the potential sensory role of odontoblasts; thus, the expression of ion channels in odontoblasts and their potential relation to Tooth Pain is also reviewed.
-
molecular mechanism for local anesthetic action of eugenol in the rat trigeminal system
Pain, 2009Co-Authors: Chulkyu Park, Sung Jun Jung, Kihwan Kim, Minji Kim, Dong Kuk Ahn, Seongdoo Hong, Joong Soo KimAbstract:Eugenol is widely used in dentistry as a local analgesic agent, because of its ability to allay Tooth Pain. Interestingly, eugenol shares several pharmacological actions with local anesthetics which include inhibition of voltage-gated sodium channel (VGSC) and activation of transient receptor potential vanilloid subtype 1 (TRPV1). In the present study, we investigated the effects of eugenol on Pain behaviors in orofacial area, and as an attempt to elucidate its mechanism we characterized inhibitory effects of eugenol on VGSCs in trigeminal ganglion (TG) neurons. TG neurons were classified into four types on the basis of their neurochemical and electrophysiological properties such as cell size, shapes of action potential (AP), isolectin-B(4) (IB(4)) binding, and were analyzed for the association of their distinctive electrophysiological properties and mRNA expression of Na(v)1.8 and TRPV1 by using single-cell RT-PCR following whole-cell recordings. Subcutaneous injection of eugenol reduced the thermal nociception and capsaicin-induced thermal hyperalgesia in a dose-dependent manner. Eugenol also diminished digastric electromyogram evoked by noxious electrical stimulation to anterior Tooth pulp, which was attributable to the blockade of AP conduction on inferior alveolar nerve. At cellular level, eugenol reversibly inhibited APs and VGSCs in IB(4)+/TRPV1+/Na(v)1.8+ nociceptive TG neurons (Type I-Type III) and IB(4)-/TRPV1-/Na(v)1.8- nociceptive TG neurons (Type IV). Both TTX-resistant I(Na) in Type I-Type III neurons and TTX-sensitive I(Na) in Type IV neurons were sensitive to eugenol. Taken together, these results suggest that eugenol may serve as local anesthetics for other pathological Pain conditions in addition to its wide use in dental clinic.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Hai-ying Li, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Seog Bae OhAbstract:Abstract Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 °, <25 °, and <17 °C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Misun Kim, Sung Joong Lee, Joong Soo KimAbstract:Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 degrees , <25 degrees , and <17 degrees C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
Joong Soo Kim - One of the best experts on this subject based on the ideXlab platform.
-
molecular mechanism for local anesthetic action of eugenol in the rat trigeminal system
Pain, 2009Co-Authors: Chulkyu Park, Sung Jun Jung, Kihwan Kim, Minji Kim, Dong Kuk Ahn, Seongdoo Hong, Joong Soo KimAbstract:Eugenol is widely used in dentistry as a local analgesic agent, because of its ability to allay Tooth Pain. Interestingly, eugenol shares several pharmacological actions with local anesthetics which include inhibition of voltage-gated sodium channel (VGSC) and activation of transient receptor potential vanilloid subtype 1 (TRPV1). In the present study, we investigated the effects of eugenol on Pain behaviors in orofacial area, and as an attempt to elucidate its mechanism we characterized inhibitory effects of eugenol on VGSCs in trigeminal ganglion (TG) neurons. TG neurons were classified into four types on the basis of their neurochemical and electrophysiological properties such as cell size, shapes of action potential (AP), isolectin-B(4) (IB(4)) binding, and were analyzed for the association of their distinctive electrophysiological properties and mRNA expression of Na(v)1.8 and TRPV1 by using single-cell RT-PCR following whole-cell recordings. Subcutaneous injection of eugenol reduced the thermal nociception and capsaicin-induced thermal hyperalgesia in a dose-dependent manner. Eugenol also diminished digastric electromyogram evoked by noxious electrical stimulation to anterior Tooth pulp, which was attributable to the blockade of AP conduction on inferior alveolar nerve. At cellular level, eugenol reversibly inhibited APs and VGSCs in IB(4)+/TRPV1+/Na(v)1.8+ nociceptive TG neurons (Type I-Type III) and IB(4)-/TRPV1-/Na(v)1.8- nociceptive TG neurons (Type IV). Both TTX-resistant I(Na) in Type I-Type III neurons and TTX-sensitive I(Na) in Type IV neurons were sensitive to eugenol. Taken together, these results suggest that eugenol may serve as local anesthetics for other pathological Pain conditions in addition to its wide use in dental clinic.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Misun Kim, Sung Joong Lee, Joong Soo KimAbstract:Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 degrees , <25 degrees , and <17 degrees C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
Sung Jun Jung - One of the best experts on this subject based on the ideXlab platform.
-
molecular mechanism for local anesthetic action of eugenol in the rat trigeminal system
Pain, 2009Co-Authors: Chulkyu Park, Sung Jun Jung, Kihwan Kim, Minji Kim, Dong Kuk Ahn, Seongdoo Hong, Joong Soo KimAbstract:Eugenol is widely used in dentistry as a local analgesic agent, because of its ability to allay Tooth Pain. Interestingly, eugenol shares several pharmacological actions with local anesthetics which include inhibition of voltage-gated sodium channel (VGSC) and activation of transient receptor potential vanilloid subtype 1 (TRPV1). In the present study, we investigated the effects of eugenol on Pain behaviors in orofacial area, and as an attempt to elucidate its mechanism we characterized inhibitory effects of eugenol on VGSCs in trigeminal ganglion (TG) neurons. TG neurons were classified into four types on the basis of their neurochemical and electrophysiological properties such as cell size, shapes of action potential (AP), isolectin-B(4) (IB(4)) binding, and were analyzed for the association of their distinctive electrophysiological properties and mRNA expression of Na(v)1.8 and TRPV1 by using single-cell RT-PCR following whole-cell recordings. Subcutaneous injection of eugenol reduced the thermal nociception and capsaicin-induced thermal hyperalgesia in a dose-dependent manner. Eugenol also diminished digastric electromyogram evoked by noxious electrical stimulation to anterior Tooth pulp, which was attributable to the blockade of AP conduction on inferior alveolar nerve. At cellular level, eugenol reversibly inhibited APs and VGSCs in IB(4)+/TRPV1+/Na(v)1.8+ nociceptive TG neurons (Type I-Type III) and IB(4)-/TRPV1-/Na(v)1.8- nociceptive TG neurons (Type IV). Both TTX-resistant I(Na) in Type I-Type III neurons and TTX-sensitive I(Na) in Type IV neurons were sensitive to eugenol. Taken together, these results suggest that eugenol may serve as local anesthetics for other pathological Pain conditions in addition to its wide use in dental clinic.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Hai-ying Li, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Seog Bae OhAbstract:Abstract Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 °, <25 °, and <17 °C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Misun Kim, Sung Joong Lee, Joong Soo KimAbstract:Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 degrees , <25 degrees , and <17 degrees C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
Donald R Nixdorf - One of the best experts on this subject based on the ideXlab platform.
-
persistent dentoalveolar Pain disorder a putative intraoral chronic overlapping Pain condition
Oral Diseases, 2019Co-Authors: Donald R Nixdorf, Alberto Herrero Babiloni, E J MoanafilhoAbstract:Chronic overlapping Pain conditions (COPCs) are conditions that share several clinical characteristics and symptomatology, are usually considered idiopathic in nature, and are frequently comorbid. Currently, there are no established inclusion criteria to determine which conditions should be included under this umbrella term despite different systems proposed. Persistent dentoalveolar Pain disorder (PDAP), also referred to as atypical odontalgia and thought to be a component of persistent idiopathic facial Pain, is a chronic Pain condition that manifests as a persistent Tooth Pain or Pain over a dentoalveolar site formerly occupied by a Tooth in the absence of detectable pathology during clinical or radiological examination. PDAP is considered idiopathic in nature, and its pathophysiological mechanisms are not fully understood. Our objective was to investigate whether PDAP fits the conceptual paradigm of COPC given its characteristics and commonalities with other COPC, based on published literature identified through a scoping review. We found that PDAP fits 16 out of 18 common characteristics among COPCs, and based on this finding, we discuss the implications of PDAP being considered a COPC.
-
Non-odontogenic “Tooth” Pain
Orofacial Disorders, 2017Co-Authors: Flavia P. Kapos, Donald R NixdorfAbstract:Non-odontogenic “Tooth” Pain characteristics are the key to identifying the underlying disorder: a detailed Pain history will guide most of the diagnostic process. Pain can be referred from local and distant structures, including musculoskeletal, neuropathic, and neurovascular sources. Response to provocation tests and administration of local anesthetic may help to rule out or confirm the origin of the Pain, as well as determine therapeutic approaches. Treatment options vary widely by diagnosis and are directed at the mechanism of the source of Pain. Missed non-odontogenic “Tooth” Pain diagnoses may result in unnecessary dental procedures, as well as continued symptomatology.
-
frequency of nonodontogenic Pain after endodontic therapy a systematic review and meta analysis
Journal of Endodontics, 2010Co-Authors: Donald R Nixdorf, E J Moanafilho, Lisa A Mcguire, James S Hodges, Alan S Law, Mike T JohnAbstract:Abstract Introduction Little is known about ill-defined Pain that persists after endodontic procedures, including an estimate of the problem's magnitude. We conducted a systematic review of prospective studies that reported the frequency of nonodontogenic Pain in patients who had undergone endodontic procedures. Methods Nonodontogenic Pain was defined as dentoalveolar Pain present for 6 months or more after endodontic treatment without evidence of dental pathology. Endodontic procedures reviewed were nonsurgical root canal treatment, retreatment, and surgical root canal treatment. Studies were searched in four databases electronically, complemented by hand searching. A summary estimate of nonodontogenic Tooth Pain frequency was derived using random-effects meta-analysis. Results Of 770 articles retrieved and reviewed, 10 met inclusion criteria, and nine had data on both odontogenic and nonodontogenic causes of Pain. A total of 3,343 teeth were enrolled within the included studies and 1,125 had follow-up information regarding Pain status. We identified 48 teeth with nonodontogenic Pain and estimated a 3.4% (95% confidence interval, 1.4%-5.5%) frequency of occurrence. In nine articles containing data regarding both odontogenic and nonodontogenic causes of Tooth Pain, 56% (44/78) of all cases were thought to have a nonodontogenic cause. Conclusions Nonodontogenic Pain is not an uncommon outcome after root canal therapy and may represent half of all cases of persistent Tooth Pain. These findings have implications for the diagnosis and treatment of Painful teeth that were previously root canal treated because therapy directed at the Tooth in question would not be expected to resolve nonodontogenic Pain.
-
frequency of persistent Tooth Pain after root canal therapy a systematic review and meta analysis
Journal of Endodontics, 2010Co-Authors: Donald R Nixdorf, E J Moanafilho, Lisa A Mcguire, James S Hodges, Mike T JohnAbstract:Abstract Introduction Little is known about the frequency of persistent Pain after endodontic procedures even though Pain is a core patient-oriented outcome. We estimated the frequency of persistent Pain, regardless of etiology, after endondontic treatment. Methods Persistent Tooth Pain was defined as Pain present ≥6 months after endodontic treatment. Endodontic procedures included in the review were pulpectomy, nonsurgical root canal treatment, surgical root canal treatment, and retreatment. Four databases were searched electronically complemented by hand searching. Two independent reviewers determined eligibility, abstracted data, and assessed study quality. A summary estimate of persistent all-cause Tooth Pain frequency was established by using a random-effects meta-analysis. Using subgroup analyses, we explored the influence of treatment approach (surgical/nonsurgical), longitudinal study design (prospective/retrospective), follow-up rate, follow-up duration, initial treatment versus retreatment, and quality of reporting (Strengthening the Reporting of Observational Studies in Epidemiology rankings) on the Pain frequency estimate. Results Of 770 articles retrieved and reviewed, 26 met inclusion criteria. A total of 5,777 teeth were enrolled, and 2,996 had follow-up information regarding Pain status. We identified 168 teeth with Pain and derived a frequency of 5.3% (95% confidence interval, 3.5%-7.2%, p I 2 = 80%) was present. In subgroup analysis, prospective studies had a higher Pain frequency (7.6%) than retrospectives studies did (0.9%). Quality of study reporting was identified as the most influential reason for study heterogeneity. Conclusions The frequency of all-cause persistent Tooth Pain after endodontic procedures was estimated to be 5.3%, with higher report quality studies suggesting >7%.
-
case series of four different headache types presenting as Tooth Pain
Journal of Endodontics, 2006Co-Authors: Aurelio A Alonso, Donald R NixdorfAbstract:Case reports in the literature discuss various headache disorders that present as Pain in the face. The current understanding of neuroanatomy and headache mechanisms suggests that headache Pain originates within intracranial structures and is then referred to the face, jaws, and teeth. This case series describes four patients, one each with migraine headache, cluster headache, paroxysmal hemicrania, and hemicrania continua, all of which who presented to dentists with the chief complaint of Tooth Pain. This is the first report of hemicrania continua presenting as Tooth Pain. It is important that dentists be cognizant of headache disorders so that they may be able to identify headache Pains masquerading as Toothache.
Seog Bae Oh - One of the best experts on this subject based on the ideXlab platform.
-
functional expression of thermo transient receptor potential channels in dental primary afferent neurons implication for Tooth Pain
Journal of Biological Chemistry, 2006Co-Authors: Chulkyu Park, Hai-ying Li, Sung Jun Jung, Zhi Fang, Seyoung Choi, Kyungpyo Park, Seog Bae OhAbstract:Abstract Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit Pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 °, <25 °, and <17 °C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to Tooth Pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of Tooth Pain.
