The Experts below are selected from a list of 255 Experts worldwide ranked by ideXlab platform

Xiangwei Li - One of the best experts on this subject based on the ideXlab platform.

  • pulp regeneration in a full length human Tooth Root using a hierarchical nanofibrous microsphere system
    Acta Biomaterialia, 2016
    Co-Authors: Xiangwei Li
    Abstract:

    Abstract While pulp regeneration using tissue engineering strategy has been explored for over a decade, successful regeneration of pulp tissues in a full-length human Root with a one-end seal that truly simulates clinical endodontic treatment has not been achieved. To address this challenge, we designed and synthesized a unique hierarchical growth factor-loaded nanofibrous microsphere scaffolding system. In this system, vascular endothelial growth factor (VEGF) binds with heparin and is encapsulated in heparin-conjugated gelatin nanospheres, which are further immobilized in the nanofibers of an injectable poly( l -lactic acid) (PLLA) microsphere. This hierarchical microsphere system not only protects the VEGF from denaturation and degradation, but also provides excellent control of its sustained release. In addition, the nanofibrous PLLA microsphere integrates the extracellular matrix-mimicking architecture with a highly porous injectable form, efficiently accommodating dental pulp stem cells (DPSCs) and supporting their proliferation and pulp tissue formation. Our in vivo study showed the successful regeneration of pulp-like tissues that fulfilled the entire apical and middle thirds and reached the coronal third of the full-length Root Canal. In addition, a large number of blood vessels were regenerated throughout the Canal. For the first time, our work demonstrates the success of pulp tissue regeneration in a full-length Root Canal, making it a significant step toward regenerative endodontics. Statement of Significance The regeneration of pulp tissues in a full-length Tooth Root Canal has been one of the greatest challenges in the field of regenerative endodontics, and one of the biggest barriers for its clinical application. In this study, we developed a unique approach to tackle this challenge, and for the first time, we successfully regenerated living pulp tissues in a full-length Root Canal, making it a significant step toward regenerative endodontics. This study will make positive scientific impact and interest the broad and multidisciplinary readership in the dental biomaterials and craniofacial tissue engineering community.

Jing Fang - One of the best experts on this subject based on the ideXlab platform.

  • cold plasma therapy of a Tooth Root Canal infected with enterococcus faecalis biofilms in vitro
    Journal of Endodontics, 2013
    Co-Authors: Yongdong Liang, Jue Zhang, Jing Fang, Jing Wang, Xiaohui Yang, K Becker
    Abstract:

    Abstract Introduction Complete sterilization of an infected Root Canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the Root Canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a Root Canal infected with Enterococcus faecalis biofilms. Methods Seventy single-Root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intraCanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and after plasma treatment. Results A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2–6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy ( P E. faecalis death and destruction of the biofilm. Conclusions The cold plasma had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental Root Canal treatment.

  • Cold plasma therapy for enterococcus faecalis biofilm infected Tooth Root Canal in vitro
    2012 Abstracts IEEE International Conference on Plasma Science, 2012
    Co-Authors: Jue Zhang, Jing Fang, Jing Wang
    Abstract:

    The inflammation of Tooth pulp or periapical tissue is the most common diseases in the dental emergency. Many studies have demonstrated that persistent endodontic infections are frequently caused by Enterococcus Faecalis (E. faecalis). When grown to biofilm, they become 1000-fold more resistant to phagocytosis, antibodies, or antimicrobials than planktonic bacteria. Eliminate and inactivate the bacteria in Root Canal system completely is the key in Root Canal treatment. In this study, the E. faecalis was incubated in the Root Canal for 7days to form the biofilm which consisted of a compact and homogeneous structure in Root Canal system. We demonstrate a new promising method for Tooth Root Canal disinfection by a single electrode based non-thermal atmospheric pressure Ar/O2 (2%) plasma source. The traditional interappointment intraCanal medication with calcium hydroxide was used as control. A significant decrease in the number of Colony-Forming Units (CFU) was observed after prolonged cold plasma treatment (from 2 minutes to 10 minutes).

  • cold plasma therapy for enterococcus faecalis biofilm infected Tooth Root Canal in vitro
    International Conference on Plasma Science, 2012
    Co-Authors: Jue Zhang, Jing Fang, Jing Wang
    Abstract:

    Summary form only given. The inflammation of Tooth pulp or periapical tissue is the most common diseases in the dental emergency. Many studies have demonstrated that persistent endodontic infections are frequently caused by Enterococcus Faecalis (E. faecalis). When grown to biofilm, they become 1000-fold more resistant to phagocytosis, antibodies, or antimicrobials than planktonic bacteria. Eliminate and inactivate the bacteria in Root Canal system completely is the key in Root Canal treatment.

  • the effect of an atmospheric pressure dc nonthermal plasma microjet on Tooth Root Canal dentinal tubules infection and reinfection prevention
    Plasma Medicine, 2011
    Co-Authors: Ruixue Wang, Jue Zhang, Haixia Zhou, Haiyan Wu, Jing Fang
    Abstract:

    A direct current, cold, atmospheric-pressure Ar/O 2 (2%) plasma microjet (PMJ) was used to disinfect Root Canals in single-Rooted extracted human teeth. Results showed that 98.8% Enterococcus faecalis (E. faecalis) was inactivated in 8 min. However, the seemingly cleaned Root Canal was reinfected after a week, possibly due to the hard-to-reach infected dentinal tubules. It was found that a 30-min PMJ treatment could effectively prevent the re- infection. This presents a simple yet effective alternative to traditional treatment of Root Canal infections in endodontic therapy.

  • Application of a non-thermal plasma to a Tooth Root Canal
    2010 Abstracts IEEE International Conference on Plasma Science, 2010
    Co-Authors: Ruixue Wang, Jue Zhang, Jing Fang, Haixia Zhou, Kurt Becker
    Abstract:

    Summary form only given. Formocresol and calcium hydroxide are commonly used as the clinical disinfectant of Root Canals. Although they have strong chemical disinfection properties, their cytotoxic effects have always been a concern. E. faecalis, which is responsible for Root Canal failure, can also cause life-threatening infections in humans, especially in the nosocomial (hospital) environment, where naturally high levels of antibiotic-resistance towards E. faecalis are found and contribute to its pathogenicity.In this study, we demonstrate a promising method for the Tooth Root Canal disinfection by an atmospheric-pressure Ar/02 (2%) cold plasma microjet (PMJ). The inactivation of E. faecalis was verified by bacterial viability test (fluorescence microscopy). Scanning electron microscopy was employed to evaluate the plasma effect on Tooth structure and the morphological changes of E. faecalis. High concentrations of OH radical were detected by Electron Spin Resonance (ESR) spectroscopy and strong atomic oxygen emissions were observed in the optical emission spectra in air. Possible pathway for the disinfection will be discussed.

Jue Zhang - One of the best experts on this subject based on the ideXlab platform.

  • cold plasma therapy of a Tooth Root Canal infected with enterococcus faecalis biofilms in vitro
    Journal of Endodontics, 2013
    Co-Authors: Yongdong Liang, Jue Zhang, Jing Fang, Jing Wang, Xiaohui Yang, K Becker
    Abstract:

    Abstract Introduction Complete sterilization of an infected Root Canal is an important challenge in endodontic treatment. Traditional methods often cannot achieve high-efficiency sterilization because of the complexity of the Root Canal system. The objective of the study was to investigate in vitro the feasibility of using a cold plasma treatment of a Root Canal infected with Enterococcus faecalis biofilms. Methods Seventy single-Root teeth infected with E. faecalis biofilms were divided into 7 groups. Group 1 served as the negative control group (no treatment), and group 7 was the positive control group with teeth treated with calcium hydroxide intraCanal medication for 7 days. Groups 2 to 6 included teeth treated by cold plasma for 2, 4, 6, 8, and 10 minutes, respectively. The disinfection of the E. faecalis biofilm was evaluated by colony-forming unit (CFU) counting. Scanning electron microscopy was used to evaluate the structural changes of the E. faecalis biofilm before and after plasma treatment. Confocal scanning laser microscopy was used to investigate the vitality of the microorganisms in the biofilm before and after plasma treatment. Results A significant decrease in the number of CFUs was observed after prolonged cold plasma treatment (based on the statistical analysis of the teeth in groups 2–6). Compared with the positive control group, cold plasma treatment of 8 or 10 minutes (groups 5 and 6) had a significantly higher antimicrobial efficacy ( P E. faecalis death and destruction of the biofilm. Conclusions The cold plasma had a high efficiency in disinfecting the E. faecalis biofilms in in vitro dental Root Canal treatment.

  • Cold plasma therapy for enterococcus faecalis biofilm infected Tooth Root Canal in vitro
    2012 Abstracts IEEE International Conference on Plasma Science, 2012
    Co-Authors: Jue Zhang, Jing Fang, Jing Wang
    Abstract:

    The inflammation of Tooth pulp or periapical tissue is the most common diseases in the dental emergency. Many studies have demonstrated that persistent endodontic infections are frequently caused by Enterococcus Faecalis (E. faecalis). When grown to biofilm, they become 1000-fold more resistant to phagocytosis, antibodies, or antimicrobials than planktonic bacteria. Eliminate and inactivate the bacteria in Root Canal system completely is the key in Root Canal treatment. In this study, the E. faecalis was incubated in the Root Canal for 7days to form the biofilm which consisted of a compact and homogeneous structure in Root Canal system. We demonstrate a new promising method for Tooth Root Canal disinfection by a single electrode based non-thermal atmospheric pressure Ar/O2 (2%) plasma source. The traditional interappointment intraCanal medication with calcium hydroxide was used as control. A significant decrease in the number of Colony-Forming Units (CFU) was observed after prolonged cold plasma treatment (from 2 minutes to 10 minutes).

  • cold plasma therapy for enterococcus faecalis biofilm infected Tooth Root Canal in vitro
    International Conference on Plasma Science, 2012
    Co-Authors: Jue Zhang, Jing Fang, Jing Wang
    Abstract:

    Summary form only given. The inflammation of Tooth pulp or periapical tissue is the most common diseases in the dental emergency. Many studies have demonstrated that persistent endodontic infections are frequently caused by Enterococcus Faecalis (E. faecalis). When grown to biofilm, they become 1000-fold more resistant to phagocytosis, antibodies, or antimicrobials than planktonic bacteria. Eliminate and inactivate the bacteria in Root Canal system completely is the key in Root Canal treatment.

  • the effect of an atmospheric pressure dc nonthermal plasma microjet on Tooth Root Canal dentinal tubules infection and reinfection prevention
    Plasma Medicine, 2011
    Co-Authors: Ruixue Wang, Jue Zhang, Haixia Zhou, Haiyan Wu, Jing Fang
    Abstract:

    A direct current, cold, atmospheric-pressure Ar/O 2 (2%) plasma microjet (PMJ) was used to disinfect Root Canals in single-Rooted extracted human teeth. Results showed that 98.8% Enterococcus faecalis (E. faecalis) was inactivated in 8 min. However, the seemingly cleaned Root Canal was reinfected after a week, possibly due to the hard-to-reach infected dentinal tubules. It was found that a 30-min PMJ treatment could effectively prevent the re- infection. This presents a simple yet effective alternative to traditional treatment of Root Canal infections in endodontic therapy.

  • Application of a non-thermal plasma to a Tooth Root Canal
    2010 Abstracts IEEE International Conference on Plasma Science, 2010
    Co-Authors: Ruixue Wang, Jue Zhang, Jing Fang, Haixia Zhou, Kurt Becker
    Abstract:

    Summary form only given. Formocresol and calcium hydroxide are commonly used as the clinical disinfectant of Root Canals. Although they have strong chemical disinfection properties, their cytotoxic effects have always been a concern. E. faecalis, which is responsible for Root Canal failure, can also cause life-threatening infections in humans, especially in the nosocomial (hospital) environment, where naturally high levels of antibiotic-resistance towards E. faecalis are found and contribute to its pathogenicity.In this study, we demonstrate a promising method for the Tooth Root Canal disinfection by an atmospheric-pressure Ar/02 (2%) cold plasma microjet (PMJ). The inactivation of E. faecalis was verified by bacterial viability test (fluorescence microscopy). Scanning electron microscopy was employed to evaluate the plasma effect on Tooth structure and the morphological changes of E. faecalis. High concentrations of OH radical were detected by Electron Spin Resonance (ESR) spectroscopy and strong atomic oxygen emissions were observed in the optical emission spectra in air. Possible pathway for the disinfection will be discussed.

Y. Matsuura - One of the best experts on this subject based on the ideXlab platform.

  • Surface modification of Tooth Root Canal after application of an X-ray opaque waveguide
    Applied Physics A, 2004
    Co-Authors: T. Dostálová, H. Jelínková, J. Šulc, P. Koranda, M. Bartoňová, P. Radina, M. Miyagi, Y. Matsuura
    Abstract:

    The interest in endodontic use of dental laser systems has been increasing. With the development of thin and flexible delivery systems for various wavelengths, laser applications in endodontics may become even more desirable. The aim of this study is to check the X-ray opacity of a hollow waveguide and to observe the results after laser Root Canal treatment. The Root Canal systems of 10 molars were treated endodontically by laser. For the laser radiation source, an Er:YAG laser system generating a wavelength of 2940 nm and an Alexandrite laser system generating a wavelength of 375 nm were used. The hollow waveguide used was checked under X-ray . A Root Canal surface treated by laser radiation was analyzed by a scanning electron microscope (SEM). The special hollow glass waveguide used was visible in the Root Canal system under X-ray imaging. Surface modification of the Root Canal after laser treatment was not found. After conventional treatment the Root Canal was enlarged. The surface was covered with a smear layer. After application of both laser systems, the smear layer was removed. The resulting Canal surface was found to be clean and smooth. Under SEM observation open dentinal tubules were visible. No cracks were present, nor were surface modifications observed.

  • surface modification of Tooth Root Canal after application of an x ray opaque waveguide
    Applied Physics A, 2004
    Co-Authors: T. Dostálová, H. Jelínková, J. Šulc, P. Koranda, M. Bartoňová, P. Radina, M. Miyagi, Y. Matsuura
    Abstract:

    The interest in endodontic use of dental laser systems has been increasing. With the development of thin and flexible delivery systems for various wavelengths, laser applications in endodontics may become even more desirable. The aim of this study is to check the X-ray opacity of a hollow waveguide and to observe the results after laser Root Canal treatment. The Root Canal systems of 10 molars were treated endodontically by laser. For the laser radiation source, an Er:YAG laser system generating a wavelength of 2940 nm and an Alexandrite laser system generating a wavelength of 375 nm were used. The hollow waveguide used was checked under X-ray . A Root Canal surface treated by laser radiation was analyzed by a scanning electron microscope (SEM). The special hollow glass waveguide used was visible in the Root Canal system under X-ray imaging.

Wei Yong - One of the best experts on this subject based on the ideXlab platform.

  • A novel image processing and measurement system applied to quantitative analysis of simulated Tooth Root Canal shape
    Electronic Imaging and Multimedia Technology IV, 2005
    Co-Authors: Tao Yong, Wei Yong
    Abstract:

    Dental pulp is located in Root Canal of Tooth. To modern Root Canal therapy, "Root Canal preparation" is the main means to debride dental pulp infection. The shape of Root Canal will be changed after preparation, so, when assessing the preparation instruments and techniques, the Root Canal shaping ability especially the apical offset is very important factor. In this paper, a novel digital image processing and measurement system is designed and applied to quantitative analysis of simulated Canal shape. By image pretreatment, feature extraction, registration and fusion, the variation of the Root Canals' characteristics (before and after preparation) can be accurately compared and measured, so as to assess the shaping ability of instruments. When the scanning resolution is 1200dpi or higher, the registration and measurement precision of the system can achieve 0.021mm or higher. The performance of the system is tested by a series of simulated Root Canals and stainless steel K-files.

  • The application of new method and system to analyze of simulated Tooth Root Canal shaping ability in stomatology
    Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, 2005
    Co-Authors: Tao Yong, Wei Yong
    Abstract:

    The study of shaping ability is very important in assessment of instruments and preparation techniques. Using simulated Canals in these studies have many advantages such as good standardization, good comparability, transparent et al. A new computer assistant measure system particularly designed for quantitative analysis of the shape of simulated Canals is set up and described in this paper. This system can be used in automatic assessment of shaping ability of instruments, by image pretreatment, feature extraction, registration, fusion, and measurement. Comparing the simulated Root Canal shape before and after instrument in the fused image is good to reduce the errors from vision and enhance the repetition of results. The registration and measurement precision of the system can achieve 0.021mm or higher, when the resolution of original Root Canal image is 1200 DPI or higher. The shaping ability of stainless steel K-files is evaluated by the system.