The Experts below are selected from a list of 1022331 Experts worldwide ranked by ideXlab platform
Jan Lötvall - One of the best experts on this subject based on the ideXlab platform.
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Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.
Scientific reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
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Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation
Scientific Reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
Aleksander Cvjetkovic - One of the best experts on this subject based on the ideXlab platform.
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Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.
Scientific reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
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Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation
Scientific Reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
Abbas Edalat - One of the best experts on this subject based on the ideXlab platform.
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LICS - The Scott Topology induces the weak Topology
Proceedings 11th Annual IEEE Symposium on Logic in Computer Science, 1996Co-Authors: Abbas EdalatAbstract:Given a probability measure on a compact metric space, we construct an increasing chain of valuations on the upper space of the metric space whose least upper bound is the measure. We then obtain the expected value of any Holder continuous function with respect to the measure up to any precision. We prove that the Scott Topology induces the weak Topology of the space of probability measures in the following general setting: Whenever a separable metric space is embedded into a subset of the maximal elements of an /spl omega/-continuous dcpo, which is a G/sub /spl delta// subset of the dcpo equipped with the Scott Topology, we show that the space of probability measures of the metric space equipped with the weak Topology is then embedded into a subspace of the maximal elements of the probabilistic power domain of the dcpo. We present a novel application in the theory of periodic doubling route to chaos.
Barbora Konečná - One of the best experts on this subject based on the ideXlab platform.
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Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.
Scientific reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
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Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation
Scientific Reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
Cecilia Lässer - One of the best experts on this subject based on the ideXlab platform.
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Detailed Analysis of Protein Topology of Extracellular Vesicles-Evidence of Unconventional Membrane Protein Orientation.
Scientific reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
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Detailed Analysis of Protein Topology of Extracellular Vesicles–Evidence of Unconventional Membrane Protein Orientation
Scientific Reports, 2016Co-Authors: Aleksander Cvjetkovic, Su Chul Jang, Barbora Konečná, Johanna L. Höög, Carina Sihlbom, Cecilia Lässer, Jan LötvallAbstract:Extracellular vesicles (EVs) are important mediators of intercellular communication that change the recipient cell by shuttling lipids, RNA, or protein cargo between cells. Here, we investigate the Topology of the protein cargo found in EVs, as this Topology can fundamentally influence the biological effects of EVs. A multiple proteomics approach, combining proteinase treatment and biotin tagging, shows that many proteins of cytosolic origin are localized on the surface of EVs. A detailed analysis of the EV proteome at the peptide level revealed that a number of EV membrane proteins are present in a topologically reversed orientation compared to what is annotated. Two examples of such proteins, SCAMP3 and STX4, were confirmed to have a reversed Topology. This reversed typology was determined using flow cytometry and fluorescent microscopy with antibodies directed toward their cytoplasmic epitopes. These results describe a novel workflow to define the EV proteome and the orientation of each protein, including membrane protein Topology. These data are fundamentally important to understanding the EV proteome and required to fully explain EV biogenesis as well as biological function in recipient cells.
