The Experts below are selected from a list of 245856 Experts worldwide ranked by ideXlab platform
Amy Ramsay - One of the best experts on this subject based on the ideXlab platform.
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Not the story you want? Assessing the fit of a conceptual framework characterising mental health recovery narratives
Social Psychiatry and Psychiatric Epidemiology, 2020Co-Authors: Joy Llewellyn-beardsley, Stefan Rennick-egglestone, Simon Bradstreet, Larry Davidson, Donna Franklin, Ada Hui, Rose Mcgranahan, Kate Morgan, Kristian Pollock, Amy RamsayAbstract:Purpose Narratives of recovery have been central to the development of the recovery approach in mental health. However, there has been a lack of clarity around definitions. A recent conceptual framework characterised recovery narratives based on a systematic review and narrative synthesis of existing literature, but was based on a limited sample. The aims of this study were to assess the relevance of the framework to the narratives of more diverse populations, and to develop a refined Typology intended to inform narrative-based research, practice and intervention development. Method 77 narrative interviews were conducted with respondents from four under-researched mental health sub-populations across England. Deductive and inductive analysis was used to assess the relevance of the dimensions and types of the preliminary Typology to the interview narratives. Results Five or more dimensions were identifiable within 97% of narratives. The preliminary Typology was refined to include new definitions and types. The Typology was found not to be relevant to two narratives, whose narrators expressed a preference for non-verbal communication. These are presented as case studies to define the limits of the Typology. Conclusion The refined Typology, based on the largest study to date of recovery narratives, provides a defensible theoretical base for clinical and research use with a range of clinical populations. Implications for practice include ensuring a heterogeneous selection of narratives as resources to support recovery, and developing new approaches to supporting non-verbal narrative construction.
James U. Bowie - One of the best experts on this subject based on the ideXlab platform.
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complete topology inversion can be part of normal membrane protein biogenesis
Protein Science, 2017Co-Authors: Nicholas B. Woodall, Ying Yin, Sarah Hadley, James U. BowieAbstract:The topology of helical membrane proteins is generally defined during insertion of the transmembrane helices, yet it is now clear that it is possible for topology to change under unusual circumstances. It remains unclear, however, if topology reorientation is part of normal biogenesis. For dual topology dimer proteins such as the multidrug transporter EmrE, there may be evolutionary pressure to allow topology flipping so that the populations of both orientations can be equalized. We previously demonstrated that when EmrE is forced to insert in a distorted topology, topology flipping of the first transmembrane helix can occur during translation. Here, we show that topological malleability also extends to the C-terminal helix and that even complete topology inversion of the entire EmrE protein can occur after the full protein is translated and inserted. Thus, topology rearrangements are possible during normal biogenesis. Wholesale topology flipping is remarkable given the physical constraints of the membrane and expands the range of possible membrane protein folding pathways, both productive and detrimental.
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Dual-topology insertion of a dual-topology membrane protein
Nature Communications, 2015Co-Authors: Nicholas B. Woodall, Ying Yin, James U. BowieAbstract:Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear. For the dual-topology transporter EmrE, the evidence to date remains consistent with two extreme models. A post-translational model posits that topology remains malleable after synthesis and becomes fixed once the dimer forms. A second, co-translational model, posits that the protein inserts in both topologies in equal proportions. Here we show that while there is at least some limited topological malleability, the co-translational model likely dominates under normal circumstances.
Joy Llewellyn-beardsley - One of the best experts on this subject based on the ideXlab platform.
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Not the story you want? Assessing the fit of a conceptual framework characterising mental health recovery narratives
Social Psychiatry and Psychiatric Epidemiology, 2020Co-Authors: Joy Llewellyn-beardsley, Stefan Rennick-egglestone, Simon Bradstreet, Larry Davidson, Donna Franklin, Ada Hui, Rose Mcgranahan, Kate Morgan, Kristian Pollock, Amy RamsayAbstract:Purpose Narratives of recovery have been central to the development of the recovery approach in mental health. However, there has been a lack of clarity around definitions. A recent conceptual framework characterised recovery narratives based on a systematic review and narrative synthesis of existing literature, but was based on a limited sample. The aims of this study were to assess the relevance of the framework to the narratives of more diverse populations, and to develop a refined Typology intended to inform narrative-based research, practice and intervention development. Method 77 narrative interviews were conducted with respondents from four under-researched mental health sub-populations across England. Deductive and inductive analysis was used to assess the relevance of the dimensions and types of the preliminary Typology to the interview narratives. Results Five or more dimensions were identifiable within 97% of narratives. The preliminary Typology was refined to include new definitions and types. The Typology was found not to be relevant to two narratives, whose narrators expressed a preference for non-verbal communication. These are presented as case studies to define the limits of the Typology. Conclusion The refined Typology, based on the largest study to date of recovery narratives, provides a defensible theoretical base for clinical and research use with a range of clinical populations. Implications for practice include ensuring a heterogeneous selection of narratives as resources to support recovery, and developing new approaches to supporting non-verbal narrative construction.
Nicholas B. Woodall - One of the best experts on this subject based on the ideXlab platform.
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complete topology inversion can be part of normal membrane protein biogenesis
Protein Science, 2017Co-Authors: Nicholas B. Woodall, Ying Yin, Sarah Hadley, James U. BowieAbstract:The topology of helical membrane proteins is generally defined during insertion of the transmembrane helices, yet it is now clear that it is possible for topology to change under unusual circumstances. It remains unclear, however, if topology reorientation is part of normal biogenesis. For dual topology dimer proteins such as the multidrug transporter EmrE, there may be evolutionary pressure to allow topology flipping so that the populations of both orientations can be equalized. We previously demonstrated that when EmrE is forced to insert in a distorted topology, topology flipping of the first transmembrane helix can occur during translation. Here, we show that topological malleability also extends to the C-terminal helix and that even complete topology inversion of the entire EmrE protein can occur after the full protein is translated and inserted. Thus, topology rearrangements are possible during normal biogenesis. Wholesale topology flipping is remarkable given the physical constraints of the membrane and expands the range of possible membrane protein folding pathways, both productive and detrimental.
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Dual-topology insertion of a dual-topology membrane protein
Nature Communications, 2015Co-Authors: Nicholas B. Woodall, Ying Yin, James U. BowieAbstract:Some membrane transporters are dual-topology dimers in which the subunits have inverted transmembrane topology. How a cell manages to generate equal populations of two opposite topologies from the same polypeptide chain remains unclear. For the dual-topology transporter EmrE, the evidence to date remains consistent with two extreme models. A post-translational model posits that topology remains malleable after synthesis and becomes fixed once the dimer forms. A second, co-translational model, posits that the protein inserts in both topologies in equal proportions. Here we show that while there is at least some limited topological malleability, the co-translational model likely dominates under normal circumstances.
Simon Bradstreet - One of the best experts on this subject based on the ideXlab platform.
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Not the story you want? Assessing the fit of a conceptual framework characterising mental health recovery narratives
Social Psychiatry and Psychiatric Epidemiology, 2020Co-Authors: Joy Llewellyn-beardsley, Stefan Rennick-egglestone, Simon Bradstreet, Larry Davidson, Donna Franklin, Ada Hui, Rose Mcgranahan, Kate Morgan, Kristian Pollock, Amy RamsayAbstract:Purpose Narratives of recovery have been central to the development of the recovery approach in mental health. However, there has been a lack of clarity around definitions. A recent conceptual framework characterised recovery narratives based on a systematic review and narrative synthesis of existing literature, but was based on a limited sample. The aims of this study were to assess the relevance of the framework to the narratives of more diverse populations, and to develop a refined Typology intended to inform narrative-based research, practice and intervention development. Method 77 narrative interviews were conducted with respondents from four under-researched mental health sub-populations across England. Deductive and inductive analysis was used to assess the relevance of the dimensions and types of the preliminary Typology to the interview narratives. Results Five or more dimensions were identifiable within 97% of narratives. The preliminary Typology was refined to include new definitions and types. The Typology was found not to be relevant to two narratives, whose narrators expressed a preference for non-verbal communication. These are presented as case studies to define the limits of the Typology. Conclusion The refined Typology, based on the largest study to date of recovery narratives, provides a defensible theoretical base for clinical and research use with a range of clinical populations. Implications for practice include ensuring a heterogeneous selection of narratives as resources to support recovery, and developing new approaches to supporting non-verbal narrative construction.
