The Experts below are selected from a list of 765 Experts worldwide ranked by ideXlab platform
H. Oberleithner - One of the best experts on this subject based on the ideXlab platform.
-
Bradykinin shifts endothelial Fluid passage from para- to Transcellular routes
Pflügers Archiv, 2006Co-Authors: C. Riethmüller, P. Jungmann, J. Wegener, H. OberleithnerAbstract:The signalling peptide bradykinin (BK) is implicated in inflammation and angiogenesis. It promotes Fluid transport from blood vessels to interstitial space, and thus facilitates oedema formation. To clarify whether paracellular or Transcellular pathways mediate this effect, we investigated the BK-stimulated Fluid transport across endothelial monolayers in vitro by comparison of electrical and fluorescence methods. Electrical cell impedance sensing monitored a biphasic response of cell layers to BK with high time resolution: a short decrease (18%, 1 min) was followed by a more sustained increase in paracellular resistance (30%, 10 min). The two phases can be assigned to second messengers of the BK-signalling pathway: Ca^2+ for the decrease and cyclic adenosine monophosphate for the rise of resistance, respectively. Despite tightening of the intercellular clefts, BK increased the Fluid permeability by 39%, indicating Transcellular Fluid transport. Additionally, BK stimulated both in- and outwardly directed membrane trafficking as assessed by vesicular Fluid uptake (by 49%) and secretion of von Willebrandt factor (by 85%). In conclusion, the combination of electrical and fluorescence data suggests that BK induces a shift from para- to Transcellular Fluid transport across endothelium.
King Helen - One of the best experts on this subject based on the ideXlab platform.
-
Fluids in human bodies and biomineralization – parallels to global water resources and reactions
2014Co-Authors: Skinner, Catherine H. W., King HelenAbstract:The amount of surface freshwaters on Earth is remarkably small considering the human population needing drinking water to survive and to ensure water in their bodies is at that very important locale where cells operate, the Transcellular Fluid. Like the Fluid in and on the planet, body Fluid is highly mobile. Cells that cause biomineralization contain and function within a Fluid fraction. The mineral bioapatite is constructed by these cells in an environment containing a small amount of Transcellular Fluid
C. Riethmüller - One of the best experts on this subject based on the ideXlab platform.
-
Bradykinin shifts endothelial Fluid passage from para- to Transcellular routes
Pflügers Archiv, 2006Co-Authors: C. Riethmüller, P. Jungmann, J. Wegener, H. OberleithnerAbstract:The signalling peptide bradykinin (BK) is implicated in inflammation and angiogenesis. It promotes Fluid transport from blood vessels to interstitial space, and thus facilitates oedema formation. To clarify whether paracellular or Transcellular pathways mediate this effect, we investigated the BK-stimulated Fluid transport across endothelial monolayers in vitro by comparison of electrical and fluorescence methods. Electrical cell impedance sensing monitored a biphasic response of cell layers to BK with high time resolution: a short decrease (18%, 1 min) was followed by a more sustained increase in paracellular resistance (30%, 10 min). The two phases can be assigned to second messengers of the BK-signalling pathway: Ca^2+ for the decrease and cyclic adenosine monophosphate for the rise of resistance, respectively. Despite tightening of the intercellular clefts, BK increased the Fluid permeability by 39%, indicating Transcellular Fluid transport. Additionally, BK stimulated both in- and outwardly directed membrane trafficking as assessed by vesicular Fluid uptake (by 49%) and secretion of von Willebrandt factor (by 85%). In conclusion, the combination of electrical and fluorescence data suggests that BK induces a shift from para- to Transcellular Fluid transport across endothelium.
Skinner, Catherine H. W. - One of the best experts on this subject based on the ideXlab platform.
-
Fluids in human bodies and biomineralization – parallels to global water resources and reactions
2014Co-Authors: Skinner, Catherine H. W., King HelenAbstract:The amount of surface freshwaters on Earth is remarkably small considering the human population needing drinking water to survive and to ensure water in their bodies is at that very important locale where cells operate, the Transcellular Fluid. Like the Fluid in and on the planet, body Fluid is highly mobile. Cells that cause biomineralization contain and function within a Fluid fraction. The mineral bioapatite is constructed by these cells in an environment containing a small amount of Transcellular Fluid
Davies S.j. - One of the best experts on this subject based on the ideXlab platform.
-
Pathways of Fluid transport and reabsorption across the peritoneal membrane
International Society of Nephrology. Published by Elsevier Inc., 2008Co-Authors: Asghar R.b., Davies S.j.Abstract:The three-pore model of peritoneal Fluid transport predicts that once the osmotic gradient has dissipated, Fluid reabsorption will be due to a combination of small-pore reabsorption driven by the intravascular oncotic pressure, and an underlying disappearance of Fluid from the cavity by lymphatic drainage. Our study measured Fluid transport by these pathways in the presence and absence of an osmotic gradient. Paired hypertonic and standard glucose-dwell studies were performed using radio-iodinated serum albumin as an intraperitoneal volume marker and changes in intraperitoneal sodium mass to determine small-pore versus Transcellular Fluid transport. Disappearance of iodinated albumin was considered to indicate lymphatic drainage. Variability in Transcellular ultrafiltration was largely explained by the rate of small-solute transport across the membrane. In the absence of an osmotic gradient, Fluid reabsorption occurred via the small-pore pathway, the rate being proportional to the small-solute transport characteristics of the membrane. In most cases, Fluid removal from the peritoneal cavity by this pathway was faster than by lymphatic drainage. Our study shows that the three-pore model describes the pathways of peritoneal Fluid transport well. In the presence of high solute transport, poor Transcellular ultrafiltration was due to loss of the osmotic gradient and an enhanced small-pore reabsorption rate after this gradient dissipated