The Experts below are selected from a list of 20103 Experts worldwide ranked by ideXlab platform

Li Wang - One of the best experts on this subject based on the ideXlab platform.

  • efficient isolation of protoplasts from freesia callus and its application in transient expression assays
    Plant Cell Tissue and Organ Culture, 2019
    Co-Authors: Xiaotong Shan, Liudi Zhou, Linna Tong, Yueqing Li, Li Wang
    Abstract:

    Freesia hybrida is one of the most famous cut flower variety domesticated from wild species in Freesia genus, distinguished for its colorful flower colors and sweet scents. It has potential to be a model plant to unravel the molecular basis of plant specialized metabolites biosynthesis in family Iridaceae, which are crucial to genetically modify ornamental traits such as flower color and flower scents. However, the lengthy life cycle propagated from tissue culture and extremely low transformation rate severely decelerate functional characterization of genes through Transgenic Method in this iridaceous plant. Using calluses induced from young flower buds from one popular cultivar of Freesia hybrida, ‘Red River®’, an efficient protoplast isolation Method was established and optimized, which was further employed in the gene transient expression system. Results demonstrated that the newly explored transient system was versatile and could be applied in numerous aspects, such as protein immunoblotting, enzyme activity, protein subcellular localization, protein–protein interaction, protein–DNA interaction and regulatory gene transregulation property assays. Moreover, calluses induced from another cultivar of Freesia hybrida, ‘Ambiance’ could also be used to isolate protoplasts for gene transient transfection analysis, indicating the wide applicability of this system. Results in this study provided new Methods for the elucidation of gene function in Freesia in vivo, which might also be inspirational in other species with long life cycles or hardly to be transformed, such as plants in family Iridaceae.

Xiaotong Shan - One of the best experts on this subject based on the ideXlab platform.

  • efficient isolation of protoplasts from freesia callus and its application in transient expression assays
    Plant Cell Tissue and Organ Culture, 2019
    Co-Authors: Xiaotong Shan, Liudi Zhou, Linna Tong, Yueqing Li, Li Wang
    Abstract:

    Freesia hybrida is one of the most famous cut flower variety domesticated from wild species in Freesia genus, distinguished for its colorful flower colors and sweet scents. It has potential to be a model plant to unravel the molecular basis of plant specialized metabolites biosynthesis in family Iridaceae, which are crucial to genetically modify ornamental traits such as flower color and flower scents. However, the lengthy life cycle propagated from tissue culture and extremely low transformation rate severely decelerate functional characterization of genes through Transgenic Method in this iridaceous plant. Using calluses induced from young flower buds from one popular cultivar of Freesia hybrida, ‘Red River®’, an efficient protoplast isolation Method was established and optimized, which was further employed in the gene transient expression system. Results demonstrated that the newly explored transient system was versatile and could be applied in numerous aspects, such as protein immunoblotting, enzyme activity, protein subcellular localization, protein–protein interaction, protein–DNA interaction and regulatory gene transregulation property assays. Moreover, calluses induced from another cultivar of Freesia hybrida, ‘Ambiance’ could also be used to isolate protoplasts for gene transient transfection analysis, indicating the wide applicability of this system. Results in this study provided new Methods for the elucidation of gene function in Freesia in vivo, which might also be inspirational in other species with long life cycles or hardly to be transformed, such as plants in family Iridaceae.

Donald D. Brown - One of the best experts on this subject based on the ideXlab platform.

  • metamorphosis is inhibited in Transgenic xenopus laevis tadpoles that overexpress type iii deiodinase
    Proceedings of the National Academy of Sciences of the United States of America, 1999
    Co-Authors: Haochu Huang, Nicholas Marsharmstrong, Donald D. Brown
    Abstract:

    One of the genes that is up-regulated by thyroid hormone (TH) during Xenopus laevis metamorphosis encodes a type III deiodinase (D3) that inactivates TH. Transgenic X. laevis tadpoles overexpressing a GFP-D3 fusion protein were produced. These Transgenic tadpoles had high levels of deiodinase activity and were resistant to exogenous TH added 1 week after fertilization. They developed normally throughout embryogenesis and premetamorphic stages but became retarded in their development late in prometamorphosis when endogenous TH reaches its highest level. Gill and tail resorption were delayed and most of the animals arrested and died. One tadpole completed its metamorphosis without resorbing its tail. These results demonstrate that D3 can modulate the action of TH in vivo, and document the value of the new Transgenic Method for functional analysis of genes involved in metamorphosis.

Sun Chunyu - One of the best experts on this subject based on the ideXlab platform.

  • study on the Transgenic royal gala apple malus domestic borkh with antifungal a thionin db4 gene
    Journal of Anhui Agricultural Sciences, 2010
    Co-Authors: Sun Chunyu
    Abstract:

    [Objective] The research aimed to improve the resistance to leaf plant disease of apple(Malus domestic Borkh) by Transgenic Method.[Method] By using Agrobacterium-mediated transformation Method,the putative Transgenic "Royal Gala" apple with antifungal α-thionin DB4 gene which driven by green specific spinach ribulose bisphosphate carboxylase/oxygenase(rubisco) activase promoter(RCAP) was obtained.[Result] PCR,Southern blotting analysis and GUS assay confirmed that α-thionin DB4 gene had been integrated into "Royal Gala" apple.[Conclusion] The Transgenic apple integrated with antifungal α-thionin DB4 gene was obtained.

Yueqing Li - One of the best experts on this subject based on the ideXlab platform.

  • efficient isolation of protoplasts from freesia callus and its application in transient expression assays
    Plant Cell Tissue and Organ Culture, 2019
    Co-Authors: Xiaotong Shan, Liudi Zhou, Linna Tong, Yueqing Li, Li Wang
    Abstract:

    Freesia hybrida is one of the most famous cut flower variety domesticated from wild species in Freesia genus, distinguished for its colorful flower colors and sweet scents. It has potential to be a model plant to unravel the molecular basis of plant specialized metabolites biosynthesis in family Iridaceae, which are crucial to genetically modify ornamental traits such as flower color and flower scents. However, the lengthy life cycle propagated from tissue culture and extremely low transformation rate severely decelerate functional characterization of genes through Transgenic Method in this iridaceous plant. Using calluses induced from young flower buds from one popular cultivar of Freesia hybrida, ‘Red River®’, an efficient protoplast isolation Method was established and optimized, which was further employed in the gene transient expression system. Results demonstrated that the newly explored transient system was versatile and could be applied in numerous aspects, such as protein immunoblotting, enzyme activity, protein subcellular localization, protein–protein interaction, protein–DNA interaction and regulatory gene transregulation property assays. Moreover, calluses induced from another cultivar of Freesia hybrida, ‘Ambiance’ could also be used to isolate protoplasts for gene transient transfection analysis, indicating the wide applicability of this system. Results in this study provided new Methods for the elucidation of gene function in Freesia in vivo, which might also be inspirational in other species with long life cycles or hardly to be transformed, such as plants in family Iridaceae.