The Experts below are selected from a list of 258 Experts worldwide ranked by ideXlab platform
Jane R. Schwebke - One of the best experts on this subject based on the ideXlab platform.
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Viability of Trichomonas vaginalis in Copan universal Transport Medium and eSwab Transport Medium.
Journal of clinical microbiology, 2008Co-Authors: Charles A. Rivers, Jane R. SchwebkeAbstract:We compared the use of universal Transport Medium and eSwab Transport Medium held at room temperature or 37°C to bedside inoculation and immediate incubation of culture media for the detection of Trichomonas vaginalis. There were no significant culturable differences in the sensitivity of either of the Transport media to that of bedside inoculation.
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Viability of Trichomonas vaginalis in Transport Medium
Journal of clinical microbiology, 1999Co-Authors: A. Beverly, M. Venglarik, B. Cotton, Jane R. SchwebkeAbstract:The ability of Amies gel agar Transport Medium to maintain the viability of Trichomonas vaginalis was determined by comparing Transported vaginal specimens to specimens immediately inoculated into culture Medium. The prevalence of trichomonosis in the study population was 26% (68 of 260 women). The immediate inoculation method detected infections in 64 of 68 infected women (sensitivity of 94.1%). The Transport method detected 62 of 68 infections (sensitivity of 91.2%). There was no significant difference between the two methods.
François Nosten - One of the best experts on this subject based on the ideXlab platform.
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Detection of Respiratory Viruses by PCR Assay of Nasopharyngeal Swabs Stored in Skim Milk-Tryptone-Glucose-Glycerol Transport Medium
Journal of clinical microbiology, 2011Co-Authors: Paul Turner, Claudia Turner, David Goldblatt, François NostenAbstract:We analyzed 129 paired nasopharyngeal aspirates (stored in viral Transport Medium [VTM]) and nasopharyngeal swabs (stored in skim milk-tryptone-glucose-glycerol [STGG] bacterial Transport and storage Medium) using PCRs to detect adenoviruses, influenza virus A or B, and respiratory syncytial virus (RSV). Overall, swabs stored in STGG Medium without antimicrobials were found to be an acceptable alternative to aspirates stored in antimicrobial-containing VTM, with PCR agreement of 90.2% (kappa of 0.8).
Marek Smieja - One of the best experts on this subject based on the ideXlab platform.
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Evaluation and Clinical Validation of an Alcohol-Based Transport Medium for Preservation and Inactivation of Respiratory Viruses
Journal of clinical microbiology, 2011Co-Authors: Kathy Luinstra, Astrid Petrich, S. Castriciano, Mona Ackerman, Sylvia Chong, Susan Carruthers, Brenna Ammons, James B. Mahony, Marek SmiejaAbstract:The clinical and public health importance of influenza and other respiratory viruses has accelerated the development of highly sensitive molecular diagnostics, but data are limited regarding preanalytical stages of diagnostic testing. We evaluated CyMol, an alcohol-based Transport Medium, for its ability to maintain specimen integrity for up to 21 days of storage at various temperatures; for its ability to inactivate virus; and for its compatibility with antigen- or nucleic acid-based diagnostics for respiratory viruses in clinical samples. In mock-infected samples, both universal Transport Medium (UTM-RT) and CyMol maintained equivalent viral quantities for at least 14 days at room temperature or colder, whereas a dry swab collection maintained viral quantities only if refrigerated or frozen. CyMol inactivated influenza virus within 5 min of sample immersion. UTM-RT- and CyMol-collected nasal swab specimens from 73 symptomatic students attending a campus health clinic were positive for a respiratory virus in 56.2% of subjects by multiplex PCR testing, including influenza A and B viruses, rhinovirus/enteroviruses, coronaviruses, respiratory syncytial virus, parainfluenza viruses, metapneumovirus, and adenovirus. Detection by PCR was equivalent in UTM-RT- and CyMol-collected specimens and in self- and staff-collected swabs. Direct fluorescent antibody (DFA) testing was substantially less sensitive (23.3%) than multiplex PCR, and DFA testing from UTM-RT-collected swabs was more sensitive than that from CyMol-collected swabs. These data indicate that an alcohol-based Transport Medium such as CyMol preserves respiratory virus integrity, rapidly inactivates viruses, and is compatible with PCR-based respiratory diagnostics.
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Use of Flocked Swabs and a Universal Transport Medium To Enhance Molecular Detection of Chlamydia trachomatis and Neisseria gonorrhoeae
Journal of clinical microbiology, 2006Co-Authors: Max Chernesky, Santina Castriciano, Dan Jang, Marek SmiejaAbstract:The use of new flocked swabs, compared to kit swabs, enhanced the ability of three commercial nucleic acid amplification tests to detect low levels of Chlamydia trachomatis and Neisseria gonorrhoeae nucleic acids when the organisms were diluted in a universal Transport Medium as mocked specimens.
Aryeh Z Baer - One of the best experts on this subject based on the ideXlab platform.
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effect of swab composition and use of swabs versus swab containing skim milk tryptone glucose glycerol stgg on culture or pcr based detection of streptococcus pneumoniae in simulated and clinical respiratory specimens in stgg Transport Medium
Journal of Clinical Microbiology, 2008Co-Authors: Lorry G Rubin, Atqia Rizvi, Aryeh Z BaerAbstract:To detect Streptococcus pneumoniae colonization, the nasopharynx is sampled using a swab placed in skim milk-tryptone-glucose-glycerol (STGG) Transport Medium, and then the swab specimen or STGG Medium is cultured or subjected to PCR. We evaluated the effect of swab composition and compared the sensitivities of detection of culture and PCR using swabs and swab-containing Medium. Calcium alginate, Dacron polyester, or rayon-tipped swabs were inoculated with pneumococci or were immersed in nasal wash specimens from children and then placed in STGG Medium. Swabs and Medium inoculated with pneumococci were cultured. Swabs grew significantly more colonies than Medium. The number of colonies cultured from rayon swabs or Medium was significantly higher than the number cultured from the calcium alginate swab or Medium. The number of colonies from both the Dacron polyester swabs and Medium were significantly lower than with either calcium alginate or rayon swabs. When DNA was separately extracted from the calcium alginate swab and Medium and subjected to PCR for pneumococcal detection from either S. pneumoniae-inoculated swabs or clinical specimens that grew S. pneumoniae, the sensitivity was at least 10 times higher using the swab. With Dacron polyester or rayon-tipped swabs, there was no consistent difference between the sensitivity of PCR using swabs and that of PCR using Medium. Thus, calcium alginate swabs may be superior to STGG Medium for the culture and PCR-based detection of S. pneumoniae. For culture, rayon swabs are superior and Dacron polyester swabs are inferior. The sensitivity of the swab and swab-containing Medium for culture or PCR detection of S. pneumoniae varies with swab composition.
Paul Turner - One of the best experts on this subject based on the ideXlab platform.
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Detection of Respiratory Viruses by PCR Assay of Nasopharyngeal Swabs Stored in Skim Milk-Tryptone-Glucose-Glycerol Transport Medium
Journal of clinical microbiology, 2011Co-Authors: Paul Turner, Claudia Turner, David Goldblatt, François NostenAbstract:We analyzed 129 paired nasopharyngeal aspirates (stored in viral Transport Medium [VTM]) and nasopharyngeal swabs (stored in skim milk-tryptone-glucose-glycerol [STGG] bacterial Transport and storage Medium) using PCRs to detect adenoviruses, influenza virus A or B, and respiratory syncytial virus (RSV). Overall, swabs stored in STGG Medium without antimicrobials were found to be an acceptable alternative to aspirates stored in antimicrobial-containing VTM, with PCR agreement of 90.2% (kappa of 0.8).
