The Experts below are selected from a list of 123 Experts worldwide ranked by ideXlab platform
Nancy W Chang - One of the best experts on this subject based on the ideXlab platform.
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increased male fertility in Tribolium confusum beetles after infection with the intracellular parasite wolbachia
Nature, 1995Co-Authors: Michael J Wade, Nancy W ChangAbstract:The cytoplasmically inherited microorganism Wolbachia pipientis behaves like a sexually selected trait in its host, the flour beetle Tribolium confusum, enhancing male fertility at the expense of female fecundity. Here we show that infected females have fewer offspring than uninfected females but infected males have a large fertility advantage over uninfected males within multiply-inseminated infected or uninfected females. The male fertility effect accelerates the spread of the Wolbachia through the host population and expands the initial opportunity for hitch-hiking of host nuclear genes. Sperm competition in a host, mediated by endosymbionts, has not been previously described.
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The transfer of Wolbachia pipientis and reproductive incompatibility between infected and uninfected strains of the flour beetle, Tribolium confusum, by microinjection
Canadian Journal of Microbiology, 1994Co-Authors: Nancy W Chang, Michael J WadeAbstract:We report a protocol for transferring the cytoplasmic endosymbiont Wolbachia pipientis from infected to uninfected hosts in the flour beetle, Tribolium confusum, by microinjection of cytoplasm from infected donor eggs into uninfected host eggs. Postinjection survivorship was limited by the halocarbon oil, which was lethal to hatching larvae but necessary to stem the backflow of cytoplasm out of injected eggs. In test crosses, approximately 39% of surviving and maturing larvae exhibited the complete reproductive incompatibility typically associated with W. pipientis in this species. However, some transfected females failed to transmit the W. pipientis to their progeny and the incompatibility could be lost in the generation following transfection. Conversely, in some cases, females that did not themselves exhibit the incompatibility had some offspring that did exhibit partial incompatibilities.Key words: microinjection, Wolbachia, flour beetle, Tribolium confusum.
S. Sabita Raja - One of the best experts on this subject based on the ideXlab platform.
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Effect of Solasodine against Last Instar Larvae of Tribolium confusum
Journal of entomology and zoology studies, 2014Co-Authors: Vasudha Lingampally, V. R. Solanki, D. L. Anuradha, S. Sabita RajaAbstract:Solasodine, a glycoalkaloid exhibited growth regulating activity in the last instar larvae of Tribolium confusum. The larvae were treated with 0.1, 0.25, 0.5, 0.75, 1, 1.5,2, 2.5, 3% concentrations of Solasodine in solvent acetone. Solasodine resulted in marked growth and developmental disruption and inhibition of metamorphosis. The larval development to adults was greatly reduced resulting in the formation of mosaics, deformed pupae, deformed adults due to interference of Solasodine with moulting process and adult eclosion. The resultant forms were ruled out from further development and reproduction. Our results suggest that Solasodine inhibits growth and development of T. confusum, suggesting its potential as an effective insect growth regulator in integrated pest management modules.
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Effect of Andrographolide on The Protein Content of Tribolium confusum (Duval)
International Journal of Pure and Applied Zoology, 2013Co-Authors: Vasudha Lingampally, V. R. Solanki, Amarjit Kaur, S. Sabita RajaAbstract:The post harvest losses and quality deterioration of food grain caused by insect pests during storage is the major problem in assuring food security in developing countries. Synthetic insecticides and fumigants have proved very effective in the control of these stored product pests. However, the problems associated with chemical insecticides such as genetic resistance by pest species, residual toxicity, increasing cost of application, pollution of the storage environment and hazard from handling directed a need for effective and biodegradable alternatives. Hence an attempt was made to control the stored product pest, Tribolium confusum using Andrographolide, the leaf extract of medicinal herb Andrographis paniculata. Its influence on the protein changes in the haemolymph, fat body and ovaries was studied. The protein content in the haemolymph, fat body and ovaries increased gradually in the larvae, pupae and the adults of Tribolium confusum, where as in Andrographolide treated resultant larvae there was a significant decrease in the protein content when compared with the controls. This sharp decline in protein content resulted in the development of various abnormalities such as larval- pupal intermediates, pupal- adult intermediates and abnormal adults.
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Andrographolide: An effective anti-fertility agent for the control of Tribolium confusum
Asian Journal of Plant Science & Research, 2012Co-Authors: Vasudha Lingampally, V. R. Solanki, S. Sabita RajaAbstract:Andrographolide, a terpenoid isolated from the leaves of Andrographis paniculata [Acanthaceae] affected normal growth and development of the ovaries of Tribolium confusum [Duval] [Coleoptera: Tenebrionidae]. The fifth instar, sixth instar larvae and pupae were treated with 1μg/μl of Andrographolide. The treated resultant females exhibited ovarian deformities. The ovaries of the treated resultant Tribolium confusum showed variation in the length and size of the ovarioles, ovariole degeneration, oocyte degeneration, resorption and inability of the mature oocytes to oviposite. Our results show that the topical application of Andrographolide inhibits ovarian development, affecting the fertility and the reproductive potentiality of Tribolium confusum suggesting its use for the development of safe and specific anti-fertility agent for the control of this stored grain pest.
F. Haghighian - One of the best experts on this subject based on the ideXlab platform.
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[Short Communication] Aِntifeedant, Growth Regulatory and Ovicidal Effect of Sambucus ebulus L. Extract on Tribolium confusum Duv.
2016Co-Authors: F. HaghighianAbstract:Aqueous extract from leaves of Sambcus ebulus L. were tested for antifeedant, growth regulation and ovicidal effects against the red floor beetle Tribolium confusum Duv. under laboratory condition (29±1ºC, RH 60±5 % and 12L:12D). The results indicated a significant deterrence for feeding, and comparatively significant larval, pupal and adult weight decrease in treated vs. controlled insects. Similarly the ovicidal effect of the extract was significant, thus reducing the number of F1 generation of resulting adults
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ِِِِ Growth Regulatory and Ovicidal Effect of Sambucus ebulus L. Extract on Tribolium confusum Duv.
2005Co-Authors: F. Haghighian, Jalal JalaliAbstract:Aqueous extract from leaves of Sambcus ebulus L. were tested for antifeedant, growth regulation and ovicidal effects against the red floor beetle Tribolium confusum Duv. under laboratory condition (29±1oC, RH 60±5% and 12L:12D). The results indicated a significant deterrence for feeding, and comparatively significant larval, pupal and adult weight decrease in treated vs. controlled insects. Similarly the ovicidal effect of the extract was significant, thus reducing the number of F1 generation of resulting adults.
A.v. Tuzinkevich - One of the best experts on this subject based on the ideXlab platform.
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Modelling of spatial-temporal dynamics of the bisexual population of Tribolium confusum
Ecological Modelling, 1991Co-Authors: A.v. TuzinkevichAbstract:Abstract The model describing an experiment which was performed by Neyman J., Park T. and Scorr E.L. (1956) to provide information about the spatial distribution of Tribolium confusum within a closed space is presented. In model distribution, the basic features of experimental data are completely reflected: concentration of individuals near the layer boundary and decrease of their density within a container. A difference in distribution patterns between males and females was mainly due to larger sensitivity of males (in comparison with females) to competitive effects of near-by individuals and, probably, lower mobility.
Michael J Wade - One of the best experts on this subject based on the ideXlab platform.
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increased male fertility in Tribolium confusum beetles after infection with the intracellular parasite wolbachia
Nature, 1995Co-Authors: Michael J Wade, Nancy W ChangAbstract:The cytoplasmically inherited microorganism Wolbachia pipientis behaves like a sexually selected trait in its host, the flour beetle Tribolium confusum, enhancing male fertility at the expense of female fecundity. Here we show that infected females have fewer offspring than uninfected females but infected males have a large fertility advantage over uninfected males within multiply-inseminated infected or uninfected females. The male fertility effect accelerates the spread of the Wolbachia through the host population and expands the initial opportunity for hitch-hiking of host nuclear genes. Sperm competition in a host, mediated by endosymbionts, has not been previously described.
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The transfer of Wolbachia pipientis and reproductive incompatibility between infected and uninfected strains of the flour beetle, Tribolium confusum, by microinjection
Canadian Journal of Microbiology, 1994Co-Authors: Nancy W Chang, Michael J WadeAbstract:We report a protocol for transferring the cytoplasmic endosymbiont Wolbachia pipientis from infected to uninfected hosts in the flour beetle, Tribolium confusum, by microinjection of cytoplasm from infected donor eggs into uninfected host eggs. Postinjection survivorship was limited by the halocarbon oil, which was lethal to hatching larvae but necessary to stem the backflow of cytoplasm out of injected eggs. In test crosses, approximately 39% of surviving and maturing larvae exhibited the complete reproductive incompatibility typically associated with W. pipientis in this species. However, some transfected females failed to transmit the W. pipientis to their progeny and the incompatibility could be lost in the generation following transfection. Conversely, in some cases, females that did not themselves exhibit the incompatibility had some offspring that did exhibit partial incompatibilities.Key words: microinjection, Wolbachia, flour beetle, Tribolium confusum.
