Tricaine

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Carine Dahl Corcini - One of the best experts on this subject based on the ideXlab platform.

  • Tricaine methanesulfonate (MS-222) on the spermatic quality of zebrafish, Danio rerio
    Aquaculture, 2021
    Co-Authors: Marina Zanin, Antonio Sergio Varela Junior, Izani Bonel Acosta, Stela Mari Meneghello Gheller, Etiane Avila Zimermann, Charles Nunes Froes, Martielo Ivan Gehrcke, Carine Dahl Corcini
    Abstract:

    Abstract Tricaine is a widely-used compound in the anesthesia of fish in both aquaculture and research laboratories. Studies on fish reproduction to date suffer from the impact of anesthetics used for euthanasia, especially the quality of gametes. Therefore, this study aims to analyze the effect of the different concentrations of Tricaine (0, 200 and 400 mg/L) on sperm kinetics parameters, structural parameters of plasma membrane fluidity and integrity, production of reactive oxygen species, mitochondrial functionality, lipoperoxidation and DNA fragmentation index by flow cytometry method. Our results showed that the concentration of 400 mg/L Tricaine (MS-222) can be used to euthanize Danio rerio even in experiments with reproductive parameters, for humanitarian reasons and avoiding animal suffering, once this concentration has not influenced kinetic parameters like sperm, plasma membrane functionality, DNA fragmentation index, reactive oxygen species and lipoperoxidation.

Tor Einar Horsberg - One of the best experts on this subject based on the ideXlab platform.

  • Behavioural Effects of the Commonly Used Fish Anaesthetic Tricaine Methanesulfonate (MS-222) on Zebrafish (Danio rerio) and Its Relevance for the Acetic Acid Pain Test
    2016
    Co-Authors: Janicke Nordgreen, A M. Tahamtani, Andrew M. Janczak, Tor Einar Horsberg
    Abstract:

    The pros and cons of using anaesthesia when handling fish in connection with experiments are debated. A widely adopted practice is to wait thirty minutes after anaesthesia before behavioural observations are initiated, but information about immediate effects of a treatment is then lost. This is pertinent for responses to acute stressors, such as acid injection in the acetic acid pain test. However, omission of anaesthetics in order to obtain data on immediate responses will compromise the welfare of fish and contribute to experimental noise due to stress. We therefore tested the effect of Tricaine methanesulfonate on the behaviour of zebrafish. We predicted that Tricaine (MS 222) would decrease swimming velocity and that the control fish would show an increased level of anxiety- and stress-related behaviours compared to the Tricaine group. Following acclimatization to the test tank, baseline behaviour was recorded before immersion in either Tricaine (168 mg l21, treatment group, N = 8) or tank water (control group, N = 7). Latencies to lose equilibrium and to lose response to touch were registered. The fish was then returned to the test tank, and the latency to regain equilibrium was registered in anaesthetized fish. When equilibrium was regained, and at five, thirty and sixty minutes after the fish had been returned to the test tank, behaviour was recorded. The Tricaine fish showed the following responses (mean 6 sd): latency to lose equilibrium 22.6 s63.9; latency to lose response to touch 101.9 s626.8; latency to regain equilibrium 92.0 s654.4. Contrary to our predictions, neither treatment caused a change in any of the behaviours registered. This indicates that Tricaine has n

  • Behavioural effects of the commonly used fish anaesthetic Tricaine methanesulfonate (MS-222) on zebrafish (Danio rerio) and its relevance for the acetic acid pain test.
    PloS one, 2014
    Co-Authors: Janicke Nordgreen, Andrew M. Janczak, Fernanda M. Tahamtani, Tor Einar Horsberg
    Abstract:

    The pros and cons of using anaesthesia when handling fish in connection with experiments are debated. A widely adopted practice is to wait thirty minutes after anaesthesia before behavioural observations are initiated, but information about immediate effects of a treatment is then lost. This is pertinent for responses to acute stressors, such as acid injection in the acetic acid pain test. However, omission of anaesthetics in order to obtain data on immediate responses will compromise the welfare of fish and contribute to experimental noise due to stress. We therefore tested the effect of Tricaine methanesulfonate on the behaviour of zebrafish. We predicted that Tricaine (MS 222) would decrease swimming velocity and that the control fish would show an increased level of anxiety- and stress-related behaviours compared to the Tricaine group. Following acclimatization to the test tank, baseline behaviour was recorded before immersion in either Tricaine (168 mg l(-1), treatment group, N = 8) or tank water (control group, N = 7). Latencies to lose equilibrium and to lose response to touch were registered. The fish was then returned to the test tank, and the latency to regain equilibrium was registered in anaesthetized fish. When equilibrium was regained, and at five, thirty and sixty minutes after the fish had been returned to the test tank, behaviour was recorded. The Tricaine fish showed the following responses (mean ± sd): latency to lose equilibrium 22.6 s±3.9; latency to lose response to touch 101.9 s±26.8; latency to regain equilibrium 92.0 s±54.4. Contrary to our predictions, neither treatment caused a change in any of the behaviours registered. This indicates that Tricaine has no effect on several commonly used behavioural parameters, and that it may be unnecessary to postpone behavioural observations to 30 min after anaesthesia.

Rachel E. Winmill - One of the best experts on this subject based on the ideXlab platform.

Marina Zanin - One of the best experts on this subject based on the ideXlab platform.

  • Tricaine methanesulfonate (MS-222) on the spermatic quality of zebrafish, Danio rerio
    Aquaculture, 2021
    Co-Authors: Marina Zanin, Antonio Sergio Varela Junior, Izani Bonel Acosta, Stela Mari Meneghello Gheller, Etiane Avila Zimermann, Charles Nunes Froes, Martielo Ivan Gehrcke, Carine Dahl Corcini
    Abstract:

    Abstract Tricaine is a widely-used compound in the anesthesia of fish in both aquaculture and research laboratories. Studies on fish reproduction to date suffer from the impact of anesthetics used for euthanasia, especially the quality of gametes. Therefore, this study aims to analyze the effect of the different concentrations of Tricaine (0, 200 and 400 mg/L) on sperm kinetics parameters, structural parameters of plasma membrane fluidity and integrity, production of reactive oxygen species, mitochondrial functionality, lipoperoxidation and DNA fragmentation index by flow cytometry method. Our results showed that the concentration of 400 mg/L Tricaine (MS-222) can be used to euthanize Danio rerio even in experiments with reproductive parameters, for humanitarian reasons and avoiding animal suffering, once this concentration has not influenced kinetic parameters like sperm, plasma membrane functionality, DNA fragmentation index, reactive oxygen species and lipoperoxidation.

Kenneth J. Rodnick - One of the best experts on this subject based on the ideXlab platform.

  • Differential effects of anesthetics on electrical properties of the rainbow trout (Oncorhynchus mykiss) heart.
    Comparative biochemistry and physiology. Part A Molecular & integrative physiology, 2006
    Co-Authors: P.a. Cotter, Kenneth J. Rodnick
    Abstract:

    We compared electrocardiographic signals in hatchery-reared, non-spinally-transected, immature rainbow trout (Oncorhynchus mykiss Walbaum) under clove oil (25 ppm), Tricaine methanesulfonate (Tricaine, 60 ppm), and benzocaine (108 ppm) general anesthesia (35 min, 14 degrees C). For all 3 anesthetics, the mean heart rate (HR) and QRS amplitude did not differ, and QRS duration and QT interval were independent of HR. Heart rate variability (HRV) was significantly (4-fold, P=0.032) higher under benzocaine than under clove oil and Tricaine, but did not differ between clove oil and Tricaine. QRS duration differed between groups (P