The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
Rosa Hermosa - One of the best experts on this subject based on the ideXlab platform.
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overexpression of the trichoderma brevicompactum tri5 gene effect on the expression of the Trichodermin biosynthetic genes and on tomato seedlings
Toxins, 2011Co-Authors: Anamariela Tijerino, Javier Moraga, Monica G Malmierca, Josefina Aleu, Isidro G Collado, Enrique Monte, Rosa Hermosa, Rosa E Cardoza, Santiago GutierrezAbstract:Trichoderma brevicompactum IBT 40841 produces Trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of Trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
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overexpression of the trichodiene synthase gene tri5 increases Trichodermin production and antimicrobial activity in trichoderma brevicompactum
Fungal Genetics and Biology, 2011Co-Authors: Anamariela Tijerino, Elena R Cardoza, Javier Moraga, Monica G Malmierca, Francisca Vicente, Josefina Aleu, Isidro G Collado, Santiago Gutierrez, Enrique Monte, Rosa HermosaAbstract:Trichoderma brevicompactum produces Trichodermin, a simple trichothecene-type toxin that shares the first steps of the sesquiterpene biosynthetic pathway with other phytotoxic trichothecenes from Fusarium spp. Trichodiene synthase catalyses the conversion of farnesyl pyrophosphate to trichodiene and it is encoded by the tri5 gene that was cloned and analysed functionally by homologous overexpression in T. brevicompactum. tri5 expression was up-regulated in media with glucose, H2O2 or glycerol. tri5 repression was observed in cultures supplemented with the antioxidants ferulic acid and tyrosol. Acetone extracts of tri5-overexpressing transformants displayed higher antifungal activity than those from the wild-type. Chromatographic and spectroscopic analyses revealed that tri5 overexpression led to an increased production of Trichodermin and tyrosol. Agar diffusion assays with these two purified metabolites from the tri5-overexpressing transformant T. brevicompactum Tb41tri5 showed that only Trichodermin had antifungal activity against Saccharomyces cerevisiae, Kluyveromyces marxianus, Candida albicans, Candida glabrata, Candida tropicalis and Aspergillus fumigatus, in most cases such activity being higher than that observed for amphotericin B and hygromycin. Our results point to the significant role of tri5 in the production of Trichodermin and in the antifungal activity of T. brevicompactum.
Santiago Gutierrez - One of the best experts on this subject based on the ideXlab platform.
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overexpression of the trichoderma brevicompactum tri5 gene effect on the expression of the Trichodermin biosynthetic genes and on tomato seedlings
Toxins, 2011Co-Authors: Anamariela Tijerino, Javier Moraga, Monica G Malmierca, Josefina Aleu, Isidro G Collado, Enrique Monte, Rosa Hermosa, Rosa E Cardoza, Santiago GutierrezAbstract:Trichoderma brevicompactum IBT 40841 produces Trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of Trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
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overexpression of the trichodiene synthase gene tri5 increases Trichodermin production and antimicrobial activity in trichoderma brevicompactum
Fungal Genetics and Biology, 2011Co-Authors: Anamariela Tijerino, Elena R Cardoza, Javier Moraga, Monica G Malmierca, Francisca Vicente, Josefina Aleu, Isidro G Collado, Santiago Gutierrez, Enrique Monte, Rosa HermosaAbstract:Trichoderma brevicompactum produces Trichodermin, a simple trichothecene-type toxin that shares the first steps of the sesquiterpene biosynthetic pathway with other phytotoxic trichothecenes from Fusarium spp. Trichodiene synthase catalyses the conversion of farnesyl pyrophosphate to trichodiene and it is encoded by the tri5 gene that was cloned and analysed functionally by homologous overexpression in T. brevicompactum. tri5 expression was up-regulated in media with glucose, H2O2 or glycerol. tri5 repression was observed in cultures supplemented with the antioxidants ferulic acid and tyrosol. Acetone extracts of tri5-overexpressing transformants displayed higher antifungal activity than those from the wild-type. Chromatographic and spectroscopic analyses revealed that tri5 overexpression led to an increased production of Trichodermin and tyrosol. Agar diffusion assays with these two purified metabolites from the tri5-overexpressing transformant T. brevicompactum Tb41tri5 showed that only Trichodermin had antifungal activity against Saccharomyces cerevisiae, Kluyveromyces marxianus, Candida albicans, Candida glabrata, Candida tropicalis and Aspergillus fumigatus, in most cases such activity being higher than that observed for amphotericin B and hygromycin. Our results point to the significant role of tri5 in the production of Trichodermin and in the antifungal activity of T. brevicompactum.
Anamariela Tijerino - One of the best experts on this subject based on the ideXlab platform.
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overexpression of the trichoderma brevicompactum tri5 gene effect on the expression of the Trichodermin biosynthetic genes and on tomato seedlings
Toxins, 2011Co-Authors: Anamariela Tijerino, Javier Moraga, Monica G Malmierca, Josefina Aleu, Isidro G Collado, Enrique Monte, Rosa Hermosa, Rosa E Cardoza, Santiago GutierrezAbstract:Trichoderma brevicompactum IBT 40841 produces Trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of Trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
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overexpression of the trichodiene synthase gene tri5 increases Trichodermin production and antimicrobial activity in trichoderma brevicompactum
Fungal Genetics and Biology, 2011Co-Authors: Anamariela Tijerino, Elena R Cardoza, Javier Moraga, Monica G Malmierca, Francisca Vicente, Josefina Aleu, Isidro G Collado, Santiago Gutierrez, Enrique Monte, Rosa HermosaAbstract:Trichoderma brevicompactum produces Trichodermin, a simple trichothecene-type toxin that shares the first steps of the sesquiterpene biosynthetic pathway with other phytotoxic trichothecenes from Fusarium spp. Trichodiene synthase catalyses the conversion of farnesyl pyrophosphate to trichodiene and it is encoded by the tri5 gene that was cloned and analysed functionally by homologous overexpression in T. brevicompactum. tri5 expression was up-regulated in media with glucose, H2O2 or glycerol. tri5 repression was observed in cultures supplemented with the antioxidants ferulic acid and tyrosol. Acetone extracts of tri5-overexpressing transformants displayed higher antifungal activity than those from the wild-type. Chromatographic and spectroscopic analyses revealed that tri5 overexpression led to an increased production of Trichodermin and tyrosol. Agar diffusion assays with these two purified metabolites from the tri5-overexpressing transformant T. brevicompactum Tb41tri5 showed that only Trichodermin had antifungal activity against Saccharomyces cerevisiae, Kluyveromyces marxianus, Candida albicans, Candida glabrata, Candida tropicalis and Aspergillus fumigatus, in most cases such activity being higher than that observed for amphotericin B and hygromycin. Our results point to the significant role of tri5 in the production of Trichodermin and in the antifungal activity of T. brevicompactum.
Xuping Shentu - One of the best experts on this subject based on the ideXlab platform.
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tri11 tri3 and tri4 genes are required for Trichodermin biosynthesis of trichoderma brevicompactum
AMB Express, 2018Co-Authors: Xuping Shentu, Jiayi Yao, Xiaofeng Yuan, Fan Sun, Kozo OchiAbstract:Trichoderma brevicompactum and T. arundinaceum both can synthesize Trichodermin with strong antifungal activity and high biotechnological value. The two Trichoderma species have a tri cluster, which includes seven genes (tri14, tri12, tri11, tri10, tri3, tri4, and tri6) that encode transport and regulatory enzymes required for the biosynthesis of Trichodermin. Here, we isolated T. brevicompactum 0248 transformants with disrupted tri11, tri4, or tri3 gene. We also described the effect of tri11, tri3, or tri4 deletion on the expression of other genes in the tri cluster. Targeted Δtri3 knockout mutant exhibited a sharp decline in the production of Trichodermin, and trichodermol, which is a substrate for Trichodermin production, accumulated. Thus, the results demonstrated that tri3 was responsible for the biosynthesis of Trichodermin, and the tri3 gene-encoded enzyme catalyzed the acetylation reaction of the hydroxy group at C-4 of the Trichodermin skeleton. In addition, tri4 and tri11 deletion mutants were generated to evaluate the roles of tri4 and tri11 in Trichodermin biosynthesis, respectively. Deletion mutant strain Δtri4 or Δtri11 did not produce Trichodermin in T. brevicompactum, indicating that tri4 and tri11 are essential for Trichodermin biosynthesis. This is the first to report the function of tri3, tri4 and tri11 in T. brevicompactum, although the role of tri4 and tri11 has already been described for T. arundinaceum by Cardoza et al. (Appl Environ Microbiol 77:4867-4877, 2011).
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transcriptome sequencing and gene expression analysis of trichoderma brevicompactum under different culture conditions
PLOS ONE, 2014Co-Authors: Xuping Shentu, Weiping Liu, Xiaohuan Zhan, Chuanxi ZhangAbstract:Background Trichoderma brevicompactum is the Trichoderma species producing simple trichothecenes-Trichodermin, a potential antifungal antibiotic and a protein synthesis inhibitor. However, the biosynthetic pathway of Trichodermin in Trichoderma is not completely clarified. Therefore, transcriptome and gene expression profiling data for this species are needed as an important resource to better understand the mechanism of the Trichodermin biosynthesis and provide a blueprint for further study of T. brevicompactum. Results In this study, de novo assembly of the T. brevicompactum transcriptome using the short-read sequencing technology (Illumina) was performed. In addition, two digital gene expression (DGE) libraries of T. brevicompactum under the Trichodermin-producing and Trichodermin-nonproducing culture conditions, respectively, were constructed to identify the differences in gene expression. A total of 23,351 unique transcripts with a mean length of 856 bp were obtained by a new Trinity de novo assembler. The variations of the gene expression under different culture conditions were also identified. The expression profiling data revealed that 3,282 unique transcripts had a significantly differential expression under the Trichodermin-producing condition, as compared to the Trichodermin-nonproducing condition. This study provides a large amount of transcript sequence data that will contribute to the study of the Trichodermin biosynthesis in T. brevicompactum. Furthermore, quantitative real-time PCR (qRT-PCR) was found to be useful to confirm the differential expression of the unique transcripts. Conclusion Our study provides considerable gene expression information of T. brevicompactum at the transcriptional level,which will help accelerate the research on the Trichodermin biosynthesis. Additionally, we have demonstrated the feasibility of using the Illumina sequencing based DGE system for gene expression profiling, and have shed new light on functional studies of the genes involved in T. brevicompactum biosynthesis.
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The Elicitation Effect of Pathogenic Fungi on Trichodermin Production by Trichoderma brevicompactum
Hindawi Limited, 2013Co-Authors: Xuping Shentu, Weiping Liu, Xiaohuan Zhan, Chuanxi ZhangAbstract:The effects of six species of phytopathogenic fungi mycelia as elicitors on Trichodermin yield by Trichoderma brevicompactum were investigated. Neither nonviable nor viable mycelia of Botrytis cinerea, Alternaria solani, Colletotrichum lindemuthianum, and Thanatephorus cucumeris demonstrated any elicitation on the accumulation of Trichodermin. However, the production of Trichodermin was increased by the presence of viable/nonviable Rhizoctonia solani and Fusarium oxysporum mycelia. The strongest elicitation effect was found at the presence of nonviable R. solani. At the presence of nonviable R. solani, the maximum yield of Trichodermin (144.55 mg/L) was significantly higher than the Control (67.8 mg/L), and the cultivation time to obtain the maximum yield of Trichodermin decreased from 72 h to 60 h. No difference of Trichodermin accumulation was observed by changing the concentration of nonviable R. solani from 0.1 to 1.6 g/L. It was observed that the optimum time for adding nonviable R. solani is immediately after inoculation. The diameter of T. brevicompactum mycelial globule after 72 h cultivation with nonviable R. solani elicitor was smaller than that of the Control
Isidro G Collado - One of the best experts on this subject based on the ideXlab platform.
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overexpression of the trichoderma brevicompactum tri5 gene effect on the expression of the Trichodermin biosynthetic genes and on tomato seedlings
Toxins, 2011Co-Authors: Anamariela Tijerino, Javier Moraga, Monica G Malmierca, Josefina Aleu, Isidro G Collado, Enrique Monte, Rosa Hermosa, Rosa E Cardoza, Santiago GutierrezAbstract:Trichoderma brevicompactum IBT 40841 produces Trichodermin, a trichothecene-type toxin that shares most of the steps of its biosynthesis with harzianum A, another trichothecene produced by several Trichoderma species. The first specific step in the trichothecene biosynthesis is carried out by a terpene cylcase, trichodiene synthase, that catalyzes the conversion of farnesyl pyrophosphate to trichodiene and that is encoded by the tri5 gene. Overexpression of tri5 resulted in increased levels of Trichodermin production, but also in an increase in tyrosol and hydroxytyrosol production, two antioxidant compounds that may play a regulatory role in trichothecene biosynthesis, and also in a higher expression of three trichothecene genes, tri4, tri6 and tri10, and of the erg1 gene, which participates in the synthesis of triterpenes. The effect of tri5 overexpression on tomato seedling disease response was also studied.
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overexpression of the trichodiene synthase gene tri5 increases Trichodermin production and antimicrobial activity in trichoderma brevicompactum
Fungal Genetics and Biology, 2011Co-Authors: Anamariela Tijerino, Elena R Cardoza, Javier Moraga, Monica G Malmierca, Francisca Vicente, Josefina Aleu, Isidro G Collado, Santiago Gutierrez, Enrique Monte, Rosa HermosaAbstract:Trichoderma brevicompactum produces Trichodermin, a simple trichothecene-type toxin that shares the first steps of the sesquiterpene biosynthetic pathway with other phytotoxic trichothecenes from Fusarium spp. Trichodiene synthase catalyses the conversion of farnesyl pyrophosphate to trichodiene and it is encoded by the tri5 gene that was cloned and analysed functionally by homologous overexpression in T. brevicompactum. tri5 expression was up-regulated in media with glucose, H2O2 or glycerol. tri5 repression was observed in cultures supplemented with the antioxidants ferulic acid and tyrosol. Acetone extracts of tri5-overexpressing transformants displayed higher antifungal activity than those from the wild-type. Chromatographic and spectroscopic analyses revealed that tri5 overexpression led to an increased production of Trichodermin and tyrosol. Agar diffusion assays with these two purified metabolites from the tri5-overexpressing transformant T. brevicompactum Tb41tri5 showed that only Trichodermin had antifungal activity against Saccharomyces cerevisiae, Kluyveromyces marxianus, Candida albicans, Candida glabrata, Candida tropicalis and Aspergillus fumigatus, in most cases such activity being higher than that observed for amphotericin B and hygromycin. Our results point to the significant role of tri5 in the production of Trichodermin and in the antifungal activity of T. brevicompactum.
