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Fahui Tang - One of the best experts on this subject based on the ideXlab platform.
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morphological redescription and molecular identification of Trichodina reticulata hirschmann partsch 1955 ciliophora mobilida trichodinidae with the supplemental new data of ssu rdna and its 5 8s rdna
Journal of Eukaryotic Microbiology, 2019Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
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Morphological Redescription and Molecular Identification of Trichodina reticulata Hirschmann & Partsch, 1955 (Ciliophora, Mobilida, Trichodinidae) with the Supplemental New Data of SSU rDNA and ITS-5.8S rDNA.
The Journal of eukaryotic microbiology, 2018Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
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Morphological and Molecular Identification of the New Species, Trichodina pseudoheterodentata sp. n. (Ciliophora, Mobilida, Trichodinidae) from the Channel Catfish, Ictalurus punctatus, in Chongqing China.
The Journal of eukaryotic microbiology, 2016Co-Authors: Fahui Tang, Yong Zhang, Yuanjun ZhaoAbstract:It is difficult to differentiate similar trichodinids solely based on morphological examination, thus other identification methods, such as molecular identification, are necessary for identification. One mobilid ciliate named Trichodina pseudoheterodentata sp. n. was isolated from the gills of channel catfish, Ictalurus punctatus, in Chongqing, China. In the present study, its SSU rDNA was sequenced for the first time. Based on the results from both morphological identification and SSU rDNA sequencing, the new species was identified and compared with similar species. The morphological analysis revealed that T. pseudoheterodentata is a large Trichodina species (cell diameter 73.0-82.5 μm) and possesses robust denticles with broad blades and well-developed blade connections. Characterization of its primary and secondary SSU rDNA structures indicated that T. pseudoheterodentata was distinctly different from congeneric species in H12, H15, E10_1, and V4 regions. Phylogenetic analysis revealed that the genetic distances among the new species and similar species reached interspecific levels, furthermore, the phylogenetic study also validated the identification of T. pseudoheterodentata and its placement in the genus Trichodina.
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Record of three new Trichodina species (Protozoa, Ciliophora) parasitic on gills of freshwater fishes from Chongqing, China
African Journal of Microbiology Research, 2013Co-Authors: Fahui Tang, Yuanjun ZhaoAbstract:The morphology of three ectoparasitic ciliates, Trichodina paraheterodentata sp. nov., Trichodina pseudominuta sp. nov. and Trichodina jialingensis sp. nov., parasitising the freshwater fishes from Chongqing, China, were investigated following dry silver nitrate and the methyl green-pyronin stain. T. paraheterodentatawas found on the skin of Siniperca chuatsi, and is distinguished mainly by the acute triangle-like blade, the well-developed central part, a little curved and robust ray. T. pseudominuta was isolated from the gills of Carassius auratus, and is characterized by the slanting parallelogram, relatively developed central part, long and slim ray arranged in helical form with the tip of ray extending past the Y+1 axis in the whole view of the parasite. T. jialingensis was collected from the gills ofCtenopharyngodon idellus, and is displaying irregular rectangular blade, undeveloped central part and the straight ray. Morphometric data and comparative descriptions of these trichodinids are provided along with details of their prevalence. Key words: Trichodina, new species, freshwater fish, Siniperca chuatsi, Carassius auratus, Ctenopharyngodon idellus, China.
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Study of Trichodinids (protozoa, Ciliophora) Parasitic on Gills of Freshwater Fishes from Chongqing, China, and Identification of a New Species Trichodina Cyprinocola Sp. Nov.
African Journal of Microbiology Research, 2011Co-Authors: Fahui Tang, Yuanjun ZhaoAbstract:This study surveyed freshwater fishes Cyprinus carpio and Carassius auratus from Chongqing, China, for infection with ectoparasitic trichodinids. Fish specimens were obtained from culture pools, smears of gills were prepared and used for silver nitrate impregnation. Microbiologic studies identified two species of trichodinids belonging to Trichodina Ehrenberg, 1838 including one new species from C. carpio that was named Trichodina cyprinocola sp. nov. Comparisons with closely related species revealed that this new species T. cyprinocola has unique sturdy denticles including the sickle blade with bluntly round tangent point, well developed central part, robust, straight but short ray. The other known species Trichodina borokensis Arthur and Lom (1984) from C. auratus was described here as the first record in China. Our survey also yielded morphometric data and prevalence of infestation for these two species.
Yuanjun Zhao - One of the best experts on this subject based on the ideXlab platform.
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morphological redescription and molecular identification of Trichodina reticulata hirschmann partsch 1955 ciliophora mobilida trichodinidae with the supplemental new data of ssu rdna and its 5 8s rdna
Journal of Eukaryotic Microbiology, 2019Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
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Morphological Redescription and Molecular Identification of Trichodina reticulata Hirschmann & Partsch, 1955 (Ciliophora, Mobilida, Trichodinidae) with the Supplemental New Data of SSU rDNA and ITS-5.8S rDNA.
The Journal of eukaryotic microbiology, 2018Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
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Morphological and Molecular Identification of the New Species, Trichodina pseudoheterodentata sp. n. (Ciliophora, Mobilida, Trichodinidae) from the Channel Catfish, Ictalurus punctatus, in Chongqing China.
The Journal of eukaryotic microbiology, 2016Co-Authors: Fahui Tang, Yong Zhang, Yuanjun ZhaoAbstract:It is difficult to differentiate similar trichodinids solely based on morphological examination, thus other identification methods, such as molecular identification, are necessary for identification. One mobilid ciliate named Trichodina pseudoheterodentata sp. n. was isolated from the gills of channel catfish, Ictalurus punctatus, in Chongqing, China. In the present study, its SSU rDNA was sequenced for the first time. Based on the results from both morphological identification and SSU rDNA sequencing, the new species was identified and compared with similar species. The morphological analysis revealed that T. pseudoheterodentata is a large Trichodina species (cell diameter 73.0-82.5 μm) and possesses robust denticles with broad blades and well-developed blade connections. Characterization of its primary and secondary SSU rDNA structures indicated that T. pseudoheterodentata was distinctly different from congeneric species in H12, H15, E10_1, and V4 regions. Phylogenetic analysis revealed that the genetic distances among the new species and similar species reached interspecific levels, furthermore, the phylogenetic study also validated the identification of T. pseudoheterodentata and its placement in the genus Trichodina.
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Record of three new Trichodina species (Protozoa, Ciliophora) parasitic on gills of freshwater fishes from Chongqing, China
African Journal of Microbiology Research, 2013Co-Authors: Fahui Tang, Yuanjun ZhaoAbstract:The morphology of three ectoparasitic ciliates, Trichodina paraheterodentata sp. nov., Trichodina pseudominuta sp. nov. and Trichodina jialingensis sp. nov., parasitising the freshwater fishes from Chongqing, China, were investigated following dry silver nitrate and the methyl green-pyronin stain. T. paraheterodentatawas found on the skin of Siniperca chuatsi, and is distinguished mainly by the acute triangle-like blade, the well-developed central part, a little curved and robust ray. T. pseudominuta was isolated from the gills of Carassius auratus, and is characterized by the slanting parallelogram, relatively developed central part, long and slim ray arranged in helical form with the tip of ray extending past the Y+1 axis in the whole view of the parasite. T. jialingensis was collected from the gills ofCtenopharyngodon idellus, and is displaying irregular rectangular blade, undeveloped central part and the straight ray. Morphometric data and comparative descriptions of these trichodinids are provided along with details of their prevalence. Key words: Trichodina, new species, freshwater fish, Siniperca chuatsi, Carassius auratus, Ctenopharyngodon idellus, China.
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Study of Trichodinids (protozoa, Ciliophora) Parasitic on Gills of Freshwater Fishes from Chongqing, China, and Identification of a New Species Trichodina Cyprinocola Sp. Nov.
African Journal of Microbiology Research, 2011Co-Authors: Fahui Tang, Yuanjun ZhaoAbstract:This study surveyed freshwater fishes Cyprinus carpio and Carassius auratus from Chongqing, China, for infection with ectoparasitic trichodinids. Fish specimens were obtained from culture pools, smears of gills were prepared and used for silver nitrate impregnation. Microbiologic studies identified two species of trichodinids belonging to Trichodina Ehrenberg, 1838 including one new species from C. carpio that was named Trichodina cyprinocola sp. nov. Comparisons with closely related species revealed that this new species T. cyprinocola has unique sturdy denticles including the sickle blade with bluntly round tangent point, well developed central part, robust, straight but short ray. The other known species Trichodina borokensis Arthur and Lom (1984) from C. auratus was described here as the first record in China. Our survey also yielded morphometric data and prevalence of infestation for these two species.
Shaobin Wang - One of the best experts on this subject based on the ideXlab platform.
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morphological redescription and molecular identification of Trichodina reticulata hirschmann partsch 1955 ciliophora mobilida trichodinidae with the supplemental new data of ssu rdna and its 5 8s rdna
Journal of Eukaryotic Microbiology, 2019Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
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Morphological Redescription and Molecular Identification of Trichodina reticulata Hirschmann & Partsch, 1955 (Ciliophora, Mobilida, Trichodinidae) with the Supplemental New Data of SSU rDNA and ITS-5.8S rDNA.
The Journal of eukaryotic microbiology, 2018Co-Authors: Shaobin Wang, Yuanjun Zhao, Fahui TangAbstract:During a survey of trichodinids in Chongqing, China, two populations of Trichodina reticulata Hirschmann & Partsch, 1955 were isolated from gills of the freshwater fishes, Carassius auratus and Ctenopharyngodon idellus, and 11 molecular samples containing small subunit (SSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA were newly sequenced. Both populations fell within the range of morphometry and corresponded well with the diagnostic morphological features of Trichodina reticulata Hirschmann & Partsch, 1955. It should be noted that one population possessed obvious central granules in the adhesive disc, while the other one did not. The detailed morphological redescription for these two populations is given in this work. Although some intraspecific differences were found, both populations were confirmed as Trichodina reticulata based on the morphological study and molecular data, including the sequence analysis of the primary and secondary structures of SSU rDNA, genetic distances and phylogenetic tree. Our study supports the assertion that central granules in the adhesive disc might be an auxiliary feature rather than a heritable character for determining taxonomic affiliations among trichodinids.
Yingchun Gong - One of the best experts on this subject based on the ideXlab platform.
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studies on trichodinid and chilodonellid ciliophorans protozoa ciliophora in the indian freshwater and estuarine fishes with description of trichodinella sunderbanensis sp nov and Trichodina nandusi sp nov
Parasitology Research, 2013Co-Authors: Amlan Kumar Mitra, Probir K. Bandyopadhyay, Yingchun GongAbstract:A comprehensive icthyoparasotological survey among estuarine and freshwater fishes of West Bengal, India revealed Trichodinella sunderbanensis sp. nov. and Trichodina acuta Lom, 1961 from an estuarine fish Mystus gulio (Hamilton, 1822) from the Vidyadhari river; Trichodina nandusi sp. nov. from a freshwater fish, Nandus nandus (Hamilton-Buchanan); Chilodonella hexasticha (Kiernik, 1909) Kahl, 1931 from freshwater fishes Labeo rohita (Hamilton, 1822); and Cyprinus carpio Linnaeus, 1758 from the Rupnarayan river. Taxonomic description of all the species based on wet silver nitrate impregnation method along with additional comments based on scanning electron microscopic descriptions of T. nandusi sp. nov. are also provided in this paper. Prevalence rate, morphometric parameters and comparisons with closely related species are also discussed.
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reconsideration of phylogenetic relationships of the subclass peritrichia ciliophora oligohymenophorea based on small subunit ribosomal rna gene sequences with the establishment of a new subclass mobilia kahl 1933
Journal of Eukaryotic Microbiology, 2009Co-Authors: Zifeng Zhan, Alan Warren, Yingchun GongAbstract:Based on its characteristic oral apparatus, the ciliate subclass Peritrichia has long been recognized as a monophyletic assemblage composed of the orders Mobilida and Sessilida. Following the application of molecular methods, the monophyly of Peritrichia has recently been questioned. We investigated the phylogenetic relationships of the peritrichous ciliates based on four further complete small subunit ribosomal RNA sequences of mobilids, namely Urceolaria urechi, Trichodina meretricis, Trichodina sinonovaculae, and Trichodina ruditapicis. In all phylogenetic trees, the mobilids never clustered with the sessilids, but instead formed a monophyletic assemblage related to the peniculines. By contrast, the sessilids formed a sister clade with the hymenostomes at a terminal position within the Oligohymenophorea. We therefore formally separate the mobilids from the sessilids (Peritrichia sensu stricto) and establish a new subclass, Mobilia Kahl, 1933, which contains the order Mobilida Kahl, 1933. We argue that the oral apparatus in the mobilians and sessilid peritrichs is a homoplasy, probably due to convergent evolution driven by their similar life-styles and feeding strategies. Morphologically, the mobilians are distinguished from all other oligohymenophoreans by the presence of the adhesive disc, this character being a synapomorphy for the Mobilia.
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Reevaluation of the phylogenetic relationship between mobilid and sessilid peritrichs (Ciliophora, Oligohymenophorea) based on small subunit rRNA genes sequences.
The Journal of eukaryotic microbiology, 2006Co-Authors: Yingchun Gong, Eduardo Villalobo, Fei-yun Zhu, Wei MiaoAbstract:Based on morphological characters, peritrich ciliates (Class Olygohymenophorea, Subclass Peritrichia) have been subdivided into the Orders Sessilida and Mobilida. Molecular phylogenetic studies on peritrichs have been restricted to members of the Order Sessilida. In order to shed more light into the evolutionary relationships within peritrichs, the complete small subunit rRNA (SSU rRNA) sequences of four mobilid species, Trichodina nobilis, Trichodina heterodentata, Trichodina reticulata, and Trichodinella myakkae were used to construct phylogenetic trees using maximum parsimony, neighbor joining, and Bayesian analyses. Whatever phylogenetic method used, the peritrichs did not constitute a monophyletic group: mobilid and sessilid species did not cluster together. Similarity in morphology but difference in molecular data led us to suggest that the oral structures of peritrichs are the result of evolutionary convergence. In addition, Trichodina reticulata, a Trichodina species with granules in the center of the adhesive disc, branched separately from its congeners, Trichodina nobilis and Trichodina heterodentata, trichodinids without such granules. This indicates that granules in the adhesive disc might be a phylogenetic character of high importance within the Family Trichodinidae.
Hao Yang - One of the best experts on this subject based on the ideXlab platform.
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Morphological and Molecular Characterization of Two Trichodina (Ciliophora, Peritrichia) Species from Freshwater Fishes in China
European journal of protistology, 2019Co-Authors: Zhe Wang, William A. Bourland, Tong Zhou, Hao Yang, Chenxin ZhangAbstract:Abstract In the present study, we provide morphological and molecular characterization of two Trichodina species, T. acuta Lom, 1970 and T. funduli Wellborn, 1967 , isolated from koi (Cyprinus carpio) and loach (Paramisgurnus dabryanus), respectively. Morphological characters of the two Trichodina species were mainly investigated on the basis of dry silver nitrate-impregnated specimens. Both species are medium-sized and possess well-developed denticles comprising strongly sickle-shaped blades, well-developed central parts, and straight rays. Trichodina acuta can be easily distinguished from the other Trichodina species that possess a clear central circle by the well-developed sharp blade apophysis, and the gap between ray tip and central circle. Trichodina funduli is a poorly known species that is easily confused with T. heterodentata Duncan, 1977, however the latter species has thinner denticles. The small subunit ribosomal RNA gene sequences of Trichodina acuta and T. funduli were incorporated into phylogenetic analyses. Our findings suggest that the phylogenetic lineage of trichodinids might not correspond with their living environments, host species or even some morphological characteristics.
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First diagnosis of ectoparasitic ciliates (Trichodina and Chilodonella) on farmed juvenile yellow catfish, Tachysurus fulvidraco in China
Aquaculture Research, 2019Co-Authors: Zhe Wang, Tong Zhou, Hao YangAbstract:Diseases caused by ectoparasitic ciliates can be a threat to yellow catfish, Tachysurus fulvidraco, an economically important fish in China. However, limited data are available regarding ectoparasitic ciliates from T. fulvidraco. In June 2015, a mass mortality event which may have been caused by ectoparasitic ciliates occurred in a population of juvenile T. fulvidraco cultured in Chidong Lake. Air‐dried smears isolated from the body surface of fish were impregnated with silver nitrate for morphological identification. In total, six known ciliate species, including four species belonging to the genus Trichodina (Trichodina acuta, Trichodina maritinkae, Trichodina nobilis, and Trichodina heterodentata) and two chilodonellids, namely, Chilodonella uncinata and Chilodonella hexasticha, and unidentified sessilids were found. Morphological descriptions of the known trichodinids and chilodonellids are provided based on silver nitrate‐impregnated specimens. Histopathological analysis revealed that trichodinids and chilodonellids could adhere to the epithelium firmly by adhesive discs and the ventral bodies, respectively, resulting in massive mucus production and epithelial hyperplasia and desquamation. This study confirmed that T. fulvidraco is a previously unrecognized host for T. acuta, T. heterodentata, T. nobilis and C. hexasticha. Although C. uncinata was also present in this study, histopathological analysis suggests that C. uncinata may be a commensal and not a true pathogen in T. fulvidraco. To our knowledge, this work is the first investigation regarding ectoparasitic ciliates on cultured T. fulvidraco in China.
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First record of two ectoparasitic ciliates of the genus Trichodina (Ciliophora: Trichodinidae) parasitizing gills of an invasive freshwater fish, Micropercops swinhonis, in Tibet.
Parasitology research, 2018Co-Authors: Zhe Wang, Tong Zhou, Qiong Deng, Hao YangAbstract:Although high diversity of parasitic ciliates has been reported in China, little is known about the species from high altitude areas, especially in Tibet. To investigate the species of parasitic ciliates in Tibet, a project was initiated in the Chabalang wetland in 2013. Two Trichodina species, namely, Trichodina sp. and T. reticulata Hirschmann & Partsch, 1955, were isolated from gills of an invasive fish, Micropercops swinhonis for the first time. In the present study, we provided the morphological, morphometrical, and molecular characterizations of the two species and conducted the phylogenetic analyses of mobilids based on the small subunit ribosomal RNA gene (SSU rDNA) sequences. Both morphological characters and morphometric data of the T. reticulata agreed well with previous studies. Although two partial SSU rDNA sequences were obtained in the present study, only the sequence of T. reticulata population in the present study was thought to be reliable. The other sequence may not belong to the other species. Thus, we regarded the other species isolated in the present study as Trichodina sp. to avoid the wrong or confused species identification. Morphologically, Trichodina sp. is distinguished mainly by its large body shape with a broad adhesive disk, robust and obliquely quadrilateral blades, and well-developed rays. T. reticulata is mainly characterized with the 8–12 spherical or elliptical granules in the central zone of adhesive disk. Phylogenetic analyses consistently showed the two ectoparasites clustered with freshwater species of the genus Trichodina within the order Mobilida. Our study extended the host range of T. reticulata and supplemented the molecular data. Also, results reveal that invasion of exotic fish may cause a potential threat to native fish by introducing or dispersing parasitic ciliates.
