The Experts below are selected from a list of 153 Experts worldwide ranked by ideXlab platform
A Rybnikár - One of the best experts on this subject based on the ideXlab platform.
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Clinical assessment of postinfection, postcontact and postvaccination immunity manifestation after experimental inoculation of calves with Trichophyton verrucosum culture.
Mycoses, 2008Co-Authors: A Rybnikár, Eva OborilovaAbstract:Immunity against ringworm has been well examined, but the knowledge of its development after repeated infection is limited. Therefore, the immunity developed after a single or double contact with various doses of Trichophyton verrucosum strain was investigated in calves which had or had not shown any signs of ringworm after contact with dermatophyte. In total six individual experiments were performed on 117 calves. Experimental infection with the virulent T. verrucosum strain was used to investigate the animals' immunity. Calves showing severe Trichophytosis after application of high doses of T. verrucosum culture were immune to reinfection by equally high doses of this dermatophyte. A similar protection level was reached after intramuscular revaccination or after recovery from Trichophytosis with moderate course induced by two inoculations of low doses. If moderate course of illness had been induced by same single dose, the immunity was lower. Animals displaying no signs of Trichophytosis after inoculation with the low doses developed insufficient immunity. There was no immunity against reinfection in animals having previous contact with subinfectious doses through undamaged skin. The results obtained suggest that the level of postinfection immunity depends on the disease extent and the frequency of administration of the infectious agent. The development of postcontact immunity cannot be excluded in calves.
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A correlation of in vitro tests for the immune response detection: A bovine Trichophytosis model
Vaccine, 2007Co-Authors: M. Faldyna, Eva Oborilova, Josef Krejci, Radek Tesarik, Eva Krejci, Barbora Pavlova, A RybnikárAbstract:The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of Trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease or challenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. Moreover the results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed vaccines.
Eva Oborilova - One of the best experts on this subject based on the ideXlab platform.
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Clinical assessment of postinfection, postcontact and postvaccination immunity manifestation after experimental inoculation of calves with Trichophyton verrucosum culture.
Mycoses, 2008Co-Authors: A Rybnikár, Eva OborilovaAbstract:Immunity against ringworm has been well examined, but the knowledge of its development after repeated infection is limited. Therefore, the immunity developed after a single or double contact with various doses of Trichophyton verrucosum strain was investigated in calves which had or had not shown any signs of ringworm after contact with dermatophyte. In total six individual experiments were performed on 117 calves. Experimental infection with the virulent T. verrucosum strain was used to investigate the animals' immunity. Calves showing severe Trichophytosis after application of high doses of T. verrucosum culture were immune to reinfection by equally high doses of this dermatophyte. A similar protection level was reached after intramuscular revaccination or after recovery from Trichophytosis with moderate course induced by two inoculations of low doses. If moderate course of illness had been induced by same single dose, the immunity was lower. Animals displaying no signs of Trichophytosis after inoculation with the low doses developed insufficient immunity. There was no immunity against reinfection in animals having previous contact with subinfectious doses through undamaged skin. The results obtained suggest that the level of postinfection immunity depends on the disease extent and the frequency of administration of the infectious agent. The development of postcontact immunity cannot be excluded in calves.
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A correlation of in vitro tests for the immune response detection: A bovine Trichophytosis model
Vaccine, 2007Co-Authors: M. Faldyna, Eva Oborilova, Josef Krejci, Radek Tesarik, Eva Krejci, Barbora Pavlova, A RybnikárAbstract:The aim of our study was to extend knowledge about possibilities of replacing challenge tests by in vitro methods in cattle on the model of Trichophytosis. We correlated results of three in vitro tests for the detection of immune response, i.e. a specific antigen-driven lymphocyte transformation test measured by (3)H-thymidine incorporation, specific antigen-induced production of interferon-gamma and detection of IgG1 and IgG2 isotypes of specific antibodies, in calves vaccinated against the disease or challenged with Trichophyton verrucosum as causative agent. The results obtained in the present study by different methods are correlated together. Lymphocyte transformation test correlated positively with interferon-gamma production. Ratio of IgG1 to IgG2 isotypes of antibody correlated negatively with both cell-mediated methods. Moreover the results show that any of the methods might in future replace the in vivo challenge tests that are still conventionally used for testing of newly developed vaccines.
Bulent Ulutas - One of the best experts on this subject based on the ideXlab platform.
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immunization with trichophyton verrucosum vaccine in hunter jumper and dressage horses with naturally occurring trichophyton equinum infection a prospective randomized double blinded placebo controlled clinical trial
Journal of Equine Veterinary Science, 2008Co-Authors: Kerem Ural, Bulent UlutasAbstract:Abstract The aim of the current study was to investigate the efficacy of Trichophyton verrucosum vaccine in hunter/jumper and dressage horses with Trichophytosis caused by Trichophyton equinum (cross immunity). A total of 25 hunter/jumper and dressage horses between the ages of 2 and 14 years, naturally infected with Trichophyton equinum, were enrolled and randomly assigned to either a placebo or an intramuscular lyophilized Trichophyton verrucosum vaccine group. Clinical evaluations were done by the same investigator, who scored clinical healing at the beginning, during, and at the end of the treatment and was blinded to allocation to the groups. At the end of the trial, vaccine treatment significantly decreased the investigator's clinical scores ( P Trichophyton verrucosum vaccine may be a safe and effective therapy for horses with Trichophyton equinum infection.
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Immunization with Trichophyton verrucosum Vaccine in Hunter/Jumper and Dressage Horses with Naturally Occurring Trichophyton equinum Infection: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Clinical Trial
Journal of Equine Veterinary Science, 2008Co-Authors: Kerem Ural, Bulent UlutasAbstract:Abstract The aim of the current study was to investigate the efficacy of Trichophyton verrucosum vaccine in hunter/jumper and dressage horses with Trichophytosis caused by Trichophyton equinum (cross immunity). A total of 25 hunter/jumper and dressage horses between the ages of 2 and 14 years, naturally infected with Trichophyton equinum, were enrolled and randomly assigned to either a placebo or an intramuscular lyophilized Trichophyton verrucosum vaccine group. Clinical evaluations were done by the same investigator, who scored clinical healing at the beginning, during, and at the end of the treatment and was blinded to allocation to the groups. At the end of the trial, vaccine treatment significantly decreased the investigator's clinical scores ( P Trichophyton verrucosum vaccine may be a safe and effective therapy for horses with Trichophyton equinum infection.
Luis González-aveledo - One of the best experts on this subject based on the ideXlab platform.
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Trichophyton -specific IgE in patients with dermatophytosis is not associated with aeroallergen sensitivity
The Journal of allergy and clinical immunology, 2000Co-Authors: Maria Teresa Escalante, Mario Sanchez-borges, Arnaldo Capriles-hulett, Edgar Belfort, Elizabeth Di Biagio, Luis González-aveledoAbstract:Abstract Background: It has been proposed that Trichophyton infection is associated with atopy and allergy. Objectives: Our purpose was (1) to confirm whether atopy predisposes to chronic dermatophytosis and (2) to investigate whether Trichophyton infection induces atopic disease. Methods: Patients attending dermatology clinics and suspected of having dermatomycosis underwent in a prospective manner fungal culture and Trichophyton and inhalant skin tests, and blood serum was collected for total IgE and Trichophyton radioallergosorbent testing. Personal and family history of atopic diseases was also investigated. Results: According to mycologic culture, atopic history, and inhalant skin test results, patients were classified into 4 groups: (1) atopy plus mycosis (n = 28), (2) atopy (n = 26), (3) mycosis (n = 35), and (4) no atopy, no mycosis (n = 33). Patients with active mycosis (groups 1 and 3) demonstrated significantly increased positivity of Trichophyton skin tests compared with patients without fungal infection (groups 2 and 4), regardless of their atopic status, whereas atopic patients (those in groups 1 and 2) had significantly increased levels of total serum IgE compared with nonatopic subjects. Trichophytosis was not more prevalent in atopic than in nonatopic subjects, and atopic diseases were not more frequent in culture-positive than in culture-negative patients. Conclusions: Our results indicate that Trichophyton -specific IgE is observed in patients with Trichophytosis regardless of atopy. (J Allergy Clin Immunol 2000;105:547-51.)
Kerem Ural - One of the best experts on this subject based on the ideXlab platform.
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immunization with trichophyton verrucosum vaccine in hunter jumper and dressage horses with naturally occurring trichophyton equinum infection a prospective randomized double blinded placebo controlled clinical trial
Journal of Equine Veterinary Science, 2008Co-Authors: Kerem Ural, Bulent UlutasAbstract:Abstract The aim of the current study was to investigate the efficacy of Trichophyton verrucosum vaccine in hunter/jumper and dressage horses with Trichophytosis caused by Trichophyton equinum (cross immunity). A total of 25 hunter/jumper and dressage horses between the ages of 2 and 14 years, naturally infected with Trichophyton equinum, were enrolled and randomly assigned to either a placebo or an intramuscular lyophilized Trichophyton verrucosum vaccine group. Clinical evaluations were done by the same investigator, who scored clinical healing at the beginning, during, and at the end of the treatment and was blinded to allocation to the groups. At the end of the trial, vaccine treatment significantly decreased the investigator's clinical scores ( P Trichophyton verrucosum vaccine may be a safe and effective therapy for horses with Trichophyton equinum infection.
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Immunization with Trichophyton verrucosum Vaccine in Hunter/Jumper and Dressage Horses with Naturally Occurring Trichophyton equinum Infection: A Prospective, Randomized, Double-Blinded, Placebo-Controlled Clinical Trial
Journal of Equine Veterinary Science, 2008Co-Authors: Kerem Ural, Bulent UlutasAbstract:Abstract The aim of the current study was to investigate the efficacy of Trichophyton verrucosum vaccine in hunter/jumper and dressage horses with Trichophytosis caused by Trichophyton equinum (cross immunity). A total of 25 hunter/jumper and dressage horses between the ages of 2 and 14 years, naturally infected with Trichophyton equinum, were enrolled and randomly assigned to either a placebo or an intramuscular lyophilized Trichophyton verrucosum vaccine group. Clinical evaluations were done by the same investigator, who scored clinical healing at the beginning, during, and at the end of the treatment and was blinded to allocation to the groups. At the end of the trial, vaccine treatment significantly decreased the investigator's clinical scores ( P Trichophyton verrucosum vaccine may be a safe and effective therapy for horses with Trichophyton equinum infection.
