The Experts below are selected from a list of 9 Experts worldwide ranked by ideXlab platform
Kristian Fog Nielsen - One of the best experts on this subject based on the ideXlab platform.
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patterns of volatile metabolites and nonvolatile Trichothecenes produced by isolates of stachybotrys fusarium trichoderma trichothecium and memnoniella
Environmental Science and Pollution Research, 2003Co-Authors: Ken Wilkins, Kristian Fog NielsenAbstract:We reported previously that trichodiene, a volatile Trichothecene Derivative, was produced by a Stachybotrys isolate, also known to produce highly cytotoxic, non-volatile, macrocyclic Trichothecenes (satrotoxins). We investigated the relationship between the production of trichodiene and various non-volatile Trichothecenes for several molds. Volatile metabolites were concentrated by adsorption on Tenax TA and analyzed by GC/MS, while non-volatile metabolites were separated by HPLC, derivatized and analyzed by GC/MS. Stachybotrys chartarum isolates producing macrocyclic Trichothecenes secreted significantly larger amounts of trichodiene and other sesquiterpenes than isolates which only produced simple Trichothecenes. The amounts of secreted trichodiene were relatively small in all cases.
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Patterns of volatile metabolites and nonvolatile Trichothecenes produced by isolates of Stachybotrys, Fusarium, Trichoderma, Trichothecium and Memnoniella.
Environmental Science and Pollution Research, 2003Co-Authors: Ken Wilkins, Kristian Fog Nielsen, Sla Ud DinAbstract:We reported previously that trichodiene, a volatile Trichothecene Derivative, was produced by a Stachybotrys isolate, also known to produce highly cytotoxic, non-volatile, macrocyclic Trichothecenes (satrotoxins). We investigated the relationship between the production of trichodiene and various non-volatile Trichothecenes for several molds. Volatile metabolites were concentrated by adsorption on Tenax TA and analyzed by GC/MS, while non-volatile metabolites were separated by HPLC, derivatized and analyzed by GC/MS. Stachybotrys chartarum isolates producing macrocyclic Trichothecenes secreted significantly larger amounts of trichodiene and other sesquiterpenes than isolates which only produced simple Trichothecenes. The amounts of secreted trichodiene were relatively small in all cases. With the exception of Memnoniella, which excreted small amounts of sesquiterpenes, the other isolates produced varying amounts of sesquiterpenes, including trichodiene, as well as simple tricothecenes, no detectable trichodiene, but large amounts of griseofulvin Derivatives. In Stachybotrys there is apparently a correlation between trichodiene and macrocyclic Trichothecene production. In the remaining isolates, there was no simple relationship between trichodiene and non-volatile Trichothecene synthesis. Trichodiene is produced in larger amounts by Stachybotrys isolates, which also produce satratoxins, but it will be difficult to utilize this metabolite to detect toxic isolates in buildings due to the relatively small amounts excreted.
Ken Wilkins - One of the best experts on this subject based on the ideXlab platform.
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patterns of volatile metabolites and nonvolatile Trichothecenes produced by isolates of stachybotrys fusarium trichoderma trichothecium and memnoniella
Environmental Science and Pollution Research, 2003Co-Authors: Ken Wilkins, Kristian Fog NielsenAbstract:We reported previously that trichodiene, a volatile Trichothecene Derivative, was produced by a Stachybotrys isolate, also known to produce highly cytotoxic, non-volatile, macrocyclic Trichothecenes (satrotoxins). We investigated the relationship between the production of trichodiene and various non-volatile Trichothecenes for several molds. Volatile metabolites were concentrated by adsorption on Tenax TA and analyzed by GC/MS, while non-volatile metabolites were separated by HPLC, derivatized and analyzed by GC/MS. Stachybotrys chartarum isolates producing macrocyclic Trichothecenes secreted significantly larger amounts of trichodiene and other sesquiterpenes than isolates which only produced simple Trichothecenes. The amounts of secreted trichodiene were relatively small in all cases.
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Patterns of volatile metabolites and nonvolatile Trichothecenes produced by isolates of Stachybotrys, Fusarium, Trichoderma, Trichothecium and Memnoniella.
Environmental Science and Pollution Research, 2003Co-Authors: Ken Wilkins, Kristian Fog Nielsen, Sla Ud DinAbstract:We reported previously that trichodiene, a volatile Trichothecene Derivative, was produced by a Stachybotrys isolate, also known to produce highly cytotoxic, non-volatile, macrocyclic Trichothecenes (satrotoxins). We investigated the relationship between the production of trichodiene and various non-volatile Trichothecenes for several molds. Volatile metabolites were concentrated by adsorption on Tenax TA and analyzed by GC/MS, while non-volatile metabolites were separated by HPLC, derivatized and analyzed by GC/MS. Stachybotrys chartarum isolates producing macrocyclic Trichothecenes secreted significantly larger amounts of trichodiene and other sesquiterpenes than isolates which only produced simple Trichothecenes. The amounts of secreted trichodiene were relatively small in all cases. With the exception of Memnoniella, which excreted small amounts of sesquiterpenes, the other isolates produced varying amounts of sesquiterpenes, including trichodiene, as well as simple tricothecenes, no detectable trichodiene, but large amounts of griseofulvin Derivatives. In Stachybotrys there is apparently a correlation between trichodiene and macrocyclic Trichothecene production. In the remaining isolates, there was no simple relationship between trichodiene and non-volatile Trichothecene synthesis. Trichodiene is produced in larger amounts by Stachybotrys isolates, which also produce satratoxins, but it will be difficult to utilize this metabolite to detect toxic isolates in buildings due to the relatively small amounts excreted.
Sla Ud Din - One of the best experts on this subject based on the ideXlab platform.
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Patterns of volatile metabolites and nonvolatile Trichothecenes produced by isolates of Stachybotrys, Fusarium, Trichoderma, Trichothecium and Memnoniella.
Environmental Science and Pollution Research, 2003Co-Authors: Ken Wilkins, Kristian Fog Nielsen, Sla Ud DinAbstract:We reported previously that trichodiene, a volatile Trichothecene Derivative, was produced by a Stachybotrys isolate, also known to produce highly cytotoxic, non-volatile, macrocyclic Trichothecenes (satrotoxins). We investigated the relationship between the production of trichodiene and various non-volatile Trichothecenes for several molds. Volatile metabolites were concentrated by adsorption on Tenax TA and analyzed by GC/MS, while non-volatile metabolites were separated by HPLC, derivatized and analyzed by GC/MS. Stachybotrys chartarum isolates producing macrocyclic Trichothecenes secreted significantly larger amounts of trichodiene and other sesquiterpenes than isolates which only produced simple Trichothecenes. The amounts of secreted trichodiene were relatively small in all cases. With the exception of Memnoniella, which excreted small amounts of sesquiterpenes, the other isolates produced varying amounts of sesquiterpenes, including trichodiene, as well as simple tricothecenes, no detectable trichodiene, but large amounts of griseofulvin Derivatives. In Stachybotrys there is apparently a correlation between trichodiene and macrocyclic Trichothecene production. In the remaining isolates, there was no simple relationship between trichodiene and non-volatile Trichothecene synthesis. Trichodiene is produced in larger amounts by Stachybotrys isolates, which also produce satratoxins, but it will be difficult to utilize this metabolite to detect toxic isolates in buildings due to the relatively small amounts excreted.
Tzong-huei Lee - One of the best experts on this subject based on the ideXlab platform.
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Efficient identification of fungal antimicrobial principles by tandem MS and NMR database
Journal of food and drug analysis, 2019Co-Authors: Ming Shian Lee, Yu-liang Yang, Ying-lien Chen, Ching Kuo Lee, S. S. Tzean, Tzong-huei LeeAbstract:Abstract The continuous re-isolation of the known and non-applicable compounds that is time-consuming and wasting resources is still a critical problem in the discovery of bioactive entities from natural resources. To efficiently address the problem, high performance liquid chromatography-diode array detector-microfractionation (HPLC-DAD-microfractionation) guided by disk agar diffusion assay was developed, and the active compounds were further identified using the tandem mass spectrometry (MS/MS)-based molecular networking. Of 150 fungal strains screened, the methanolic extracts of Phoma herbarum PPM7487, Cryptosporiopsis ericae PPM7405, and Albifimbria verrucaria PPM945 exhibited potent antimicrobial activity against Candida albicans SC5314 and Cryptococcus neoformans H99 in the preliminary agar diffusion assay. The concept of OSMAC (one strain many compounds) was employed in the fungal cultures in order to enrich the diversity of the fungal 2nd metabolites in this study. HPLC coupled with off-line bioactivity-directed profiling of the extracts enabled a precise localization of the compounds responsible for the conspicuous antimicrobial activity. The purified active compounds were identified based mainly on MS/MS database, and further supported by 13 C nuclear magnetic resonance (NMR) spectral data compared to the literatures. In addition to nineteen known compounds, a new Trichothecene Derivative 1 , namely trichoverrin D, was isolated and identified through this protocol. The antifungal activities of all the pure isolates were evaluated, and the structure activity relationships were also inferred. This report has demonstrated the combination of HPLC microfractination and MS/MS coupled by NMR spectral dereplication for speeding up the antimicrobial natural products discovery process.
Ming Shian Lee - One of the best experts on this subject based on the ideXlab platform.
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Efficient identification of fungal antimicrobial principles by tandem MS and NMR database
Journal of food and drug analysis, 2019Co-Authors: Ming Shian Lee, Yu-liang Yang, Ying-lien Chen, Ching Kuo Lee, S. S. Tzean, Tzong-huei LeeAbstract:Abstract The continuous re-isolation of the known and non-applicable compounds that is time-consuming and wasting resources is still a critical problem in the discovery of bioactive entities from natural resources. To efficiently address the problem, high performance liquid chromatography-diode array detector-microfractionation (HPLC-DAD-microfractionation) guided by disk agar diffusion assay was developed, and the active compounds were further identified using the tandem mass spectrometry (MS/MS)-based molecular networking. Of 150 fungal strains screened, the methanolic extracts of Phoma herbarum PPM7487, Cryptosporiopsis ericae PPM7405, and Albifimbria verrucaria PPM945 exhibited potent antimicrobial activity against Candida albicans SC5314 and Cryptococcus neoformans H99 in the preliminary agar diffusion assay. The concept of OSMAC (one strain many compounds) was employed in the fungal cultures in order to enrich the diversity of the fungal 2nd metabolites in this study. HPLC coupled with off-line bioactivity-directed profiling of the extracts enabled a precise localization of the compounds responsible for the conspicuous antimicrobial activity. The purified active compounds were identified based mainly on MS/MS database, and further supported by 13 C nuclear magnetic resonance (NMR) spectral data compared to the literatures. In addition to nineteen known compounds, a new Trichothecene Derivative 1 , namely trichoverrin D, was isolated and identified through this protocol. The antifungal activities of all the pure isolates were evaluated, and the structure activity relationships were also inferred. This report has demonstrated the combination of HPLC microfractination and MS/MS coupled by NMR spectral dereplication for speeding up the antimicrobial natural products discovery process.
