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Yang Bi - One of the best experts on this subject based on the ideXlab platform.
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possible effect of hydrogen peroxide generated by Trichothecium roseum in pathogenicity
Caribbean Journal of Science, 2019Co-Authors: Yang Bi, Yi Wang, Jing Wang, Weibing ZhangAbstract:In order to investigate the production and effect of hydrogen peroxide (H2O2) in Trichothecium roseum, the histochemical staining of transmission electron microscope and horseradish peroxidase phenol red methods were used to detected the production and location of H2O2 in T. roseum, and the relationship between the H2O2 generated by T. roseum and its pathogenicity was detected using H2O2 scavenger, dimethylthiourea (DMTU) and glucose oxidase (GO) which sustainably produces exogenous H2O2 in culture medium. Our results showed that the conidia of T. roseum could generate a large number of H2O2 in the cell wall and germination tube during the spore germination and growth process. DMTU could effectively remove the H2O2 generated by the mycelium (P < 0.05), and significantly inhibited the growth of the mycelium, the inhibition rate was 63.24% after 7days of cultivation, the conidia production of T. roseum was also inhibited by DMTU treatment, the inhibition rate was 43.39% after 3days of cultivation. Significantly, apple fruit were inoculated with T. roseum spores suspension treated with DMTU, the activity of cellulase (Cx) and polygalacturonase (PG) were also inhibited. At 7days post-inoculation, compared with the control, the enzymatic activity of Cx and PG decreased by 68.03% and 58.96%, respectively, and the rotted spot diameter decreased by 48.28%. The pathogenicity of T. roseum spores was weakened after DMTU treatment. Supplementations of glucose oxidase (GO) could alleviate the inhibitory effect of pathogenicity of T. roseum spores which were treated by DMTU to remove H2O2. The results suggested that T. roseum conidia could produces H2O2 during germination, and DMTU can remove its H2O2, weaken the growth of mycelium, decrease the activity of cell wall degrading enzyme Cx and PG, and reduce the pathogenicity. The effect of H2O2 on the pathogenicity of T. roseum may be achieved by reducing the growth of mycelia and reducing the activity of cell wall degrading enzymes.
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Trichothecium roseum infection promotes ripening of harvested muskmelon fruits and induces the release of specific volatile compound
Journal of Plant Pathology, 2019Co-Authors: Di Gong, Shenge Li, Yongcai Li, Yang Bi, Yi WangAbstract:Pink rot caused by Trichothecium roseum is a major postharvest disease of muskmelon fruit in the northwestern areas of China. The pathogen generally reduced quality of melons and enhanced loss. In this study, T. roseum infected muskmelon (Cucumis melo c v. ‘Jinhongbao’) fruits were used to investigate the effect of infection on respiration rate, ethylene production, basic quality parameters and volatile compounds. The results showed that T. roseum infection increased an earlier ethylene and respiration evolution of the infected fruits. Infected fruit showed an higher weight loss, but lower firmness, total soluble solid (TSS) and ratio of TSS to titratable acid (TA). The amounts of total volatile compounds, aldehydes, alcohols and esters, increased following infection. Among them, heptanal, benzeneacetaldehyde and 4-methyl-2-propyl-1-pentanol were specifically found in the infected fruits. Between the produced volatiles, heptanal and 4-methyl-2-propyl-1-pentanol showed an increase, while benzeneacetaldehyde showed a downward trend over the whole infection period. These results suggested that T. roseum infection promotes muskmelon fruit ripening and quality loss, accelerates release of esters, aldehydes, and alcohols, and produces some unique volatile compounds.
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early defense responses involved in mitochondrial energy metabolism and reactive oxygen species accumulation in harvested muskmelons infected by Trichothecium roseum
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Yang Bi, Shenge Li, Zhong Zhang, Dov PruskyAbstract:Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H2O2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase. However, the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transpor...
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influence of storage temperature and cultivars on t 2 toxin and neosolaniol accumulation in apples inoculated with Trichothecium roseum
Journal of Food Processing and Preservation, 2018Co-Authors: Yang Bi, Shivappa Hukkeri, L Pu, G. Fu, Y. Tang, Yi Wang, X Li, Y LiAbstract:Trichothecium roseum is one of the most dreadful postharvest pathogens responsible for core rot of apples. Besides causing economic loss, the disease is also associated with mycotoxins contamination. The effects of storage temperature and lesion diameter on T-2 toxin and neosolaniol (NEO) accumulation were evaluated in genetically diverse apple cultivars. The results showed T-2 concentration was much higher in Fuji than that in Ralls, while NEO was only detected in Red Delicious. The room temperature is favorable for T-2 and NEO accumulation compared with low temperature. We also noticed the positive relationship between the size of lesion diameter and the concentrations of NEO and T-2. NEO and T-2 were found not only in the rotten part, but in the adjacent asymptomatic tissue, indicating that the spread of the trichothecenes to non-infected of the fruits, and trichothecenes concentration showed a trend of decline with increase in distance from the rotten parts. Practical application Trichothecium roseum is one of the most important postharvest pathogens, and can cause core rot of apples. The disease not only causes economic loss, but also results in trichothecene contamination. Storage temperature and the size of lesion diameter significantly affected T-2 and neosolaniol accumulation in genetically diverse apple. The results showed T-2 toxin concentration was much higher in Fuji than that in Ralls, while neosolaniol was only detected in Red Delicious. The room temperature was favorable for T-2 toxin and neosolaniol accumulation compared with low temperature. In addition, the positive relationship between the size of lesion diameter and the concentrations of neosolaniol and T-2 toxin was observed. T-2 toxin and neosolaniol were found not only in the rotten part, but also in the adjacent asymptomatic tissue, and the concentration showed a trend of decline with increase in distance from the rotten parts. The study will be useful not only for apple post-harvest storage and reduce the health risk of exposure to the trichothecenes of T-2 toxin and neosolaniol, but also provide baseline information for the introduction of EU-limits for T-2 toxin and neosolaniol.
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damage to Trichothecium roseum caused by sodium silicate is independent from ph
Canadian Journal of Microbiology, 2016Co-Authors: Yang Bi, Shenggui Zhang, Yongcai Li, Yi Wang, Alejandro CalderonurreaAbstract:Trichothecium roseum is one of the most important postharvest pathogens in arid and semiarid regions. Sodium silicate (NaSi) and environmental pH have significant inhibitory effects on fungal growth. However, no study has addressed the relationship of NaSi and pH in combination and the effects on T. roseum. In this work, we showed that spore germination, germ tube elongation, and mycelial growth of T. roseum were significantly inhibited by various NaSi concentrations, which had corresponding increasing pHs. Furthermore, these NaSi solutions showed a much greater impact than did pH treatments alone. The pathogenicity of NaSi-treated conidia on a model assay (conidia-inoculated apple fruit) was dramatically reduced, whereas no changes of pathogenicity were evident for the corresponding pH (various sodium hydroxide (NaOH) solutions) treatments. Fluorescent microscopy, using propidium iodide staining, showed damage of the plasma membranes of T. roseum conidia treated with both NaSi and NaOH, although the dama...
Yi Wang - One of the best experts on this subject based on the ideXlab platform.
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possible effect of hydrogen peroxide generated by Trichothecium roseum in pathogenicity
Caribbean Journal of Science, 2019Co-Authors: Yang Bi, Yi Wang, Jing Wang, Weibing ZhangAbstract:In order to investigate the production and effect of hydrogen peroxide (H2O2) in Trichothecium roseum, the histochemical staining of transmission electron microscope and horseradish peroxidase phenol red methods were used to detected the production and location of H2O2 in T. roseum, and the relationship between the H2O2 generated by T. roseum and its pathogenicity was detected using H2O2 scavenger, dimethylthiourea (DMTU) and glucose oxidase (GO) which sustainably produces exogenous H2O2 in culture medium. Our results showed that the conidia of T. roseum could generate a large number of H2O2 in the cell wall and germination tube during the spore germination and growth process. DMTU could effectively remove the H2O2 generated by the mycelium (P < 0.05), and significantly inhibited the growth of the mycelium, the inhibition rate was 63.24% after 7days of cultivation, the conidia production of T. roseum was also inhibited by DMTU treatment, the inhibition rate was 43.39% after 3days of cultivation. Significantly, apple fruit were inoculated with T. roseum spores suspension treated with DMTU, the activity of cellulase (Cx) and polygalacturonase (PG) were also inhibited. At 7days post-inoculation, compared with the control, the enzymatic activity of Cx and PG decreased by 68.03% and 58.96%, respectively, and the rotted spot diameter decreased by 48.28%. The pathogenicity of T. roseum spores was weakened after DMTU treatment. Supplementations of glucose oxidase (GO) could alleviate the inhibitory effect of pathogenicity of T. roseum spores which were treated by DMTU to remove H2O2. The results suggested that T. roseum conidia could produces H2O2 during germination, and DMTU can remove its H2O2, weaken the growth of mycelium, decrease the activity of cell wall degrading enzyme Cx and PG, and reduce the pathogenicity. The effect of H2O2 on the pathogenicity of T. roseum may be achieved by reducing the growth of mycelia and reducing the activity of cell wall degrading enzymes.
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Trichothecium roseum infection promotes ripening of harvested muskmelon fruits and induces the release of specific volatile compound
Journal of Plant Pathology, 2019Co-Authors: Di Gong, Shenge Li, Yongcai Li, Yang Bi, Yi WangAbstract:Pink rot caused by Trichothecium roseum is a major postharvest disease of muskmelon fruit in the northwestern areas of China. The pathogen generally reduced quality of melons and enhanced loss. In this study, T. roseum infected muskmelon (Cucumis melo c v. ‘Jinhongbao’) fruits were used to investigate the effect of infection on respiration rate, ethylene production, basic quality parameters and volatile compounds. The results showed that T. roseum infection increased an earlier ethylene and respiration evolution of the infected fruits. Infected fruit showed an higher weight loss, but lower firmness, total soluble solid (TSS) and ratio of TSS to titratable acid (TA). The amounts of total volatile compounds, aldehydes, alcohols and esters, increased following infection. Among them, heptanal, benzeneacetaldehyde and 4-methyl-2-propyl-1-pentanol were specifically found in the infected fruits. Between the produced volatiles, heptanal and 4-methyl-2-propyl-1-pentanol showed an increase, while benzeneacetaldehyde showed a downward trend over the whole infection period. These results suggested that T. roseum infection promotes muskmelon fruit ripening and quality loss, accelerates release of esters, aldehydes, and alcohols, and produces some unique volatile compounds.
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influence of storage temperature and cultivars on t 2 toxin and neosolaniol accumulation in apples inoculated with Trichothecium roseum
Journal of Food Processing and Preservation, 2018Co-Authors: Yang Bi, Shivappa Hukkeri, L Pu, G. Fu, Y. Tang, Yi Wang, X Li, Y LiAbstract:Trichothecium roseum is one of the most dreadful postharvest pathogens responsible for core rot of apples. Besides causing economic loss, the disease is also associated with mycotoxins contamination. The effects of storage temperature and lesion diameter on T-2 toxin and neosolaniol (NEO) accumulation were evaluated in genetically diverse apple cultivars. The results showed T-2 concentration was much higher in Fuji than that in Ralls, while NEO was only detected in Red Delicious. The room temperature is favorable for T-2 and NEO accumulation compared with low temperature. We also noticed the positive relationship between the size of lesion diameter and the concentrations of NEO and T-2. NEO and T-2 were found not only in the rotten part, but in the adjacent asymptomatic tissue, indicating that the spread of the trichothecenes to non-infected of the fruits, and trichothecenes concentration showed a trend of decline with increase in distance from the rotten parts. Practical application Trichothecium roseum is one of the most important postharvest pathogens, and can cause core rot of apples. The disease not only causes economic loss, but also results in trichothecene contamination. Storage temperature and the size of lesion diameter significantly affected T-2 and neosolaniol accumulation in genetically diverse apple. The results showed T-2 toxin concentration was much higher in Fuji than that in Ralls, while neosolaniol was only detected in Red Delicious. The room temperature was favorable for T-2 toxin and neosolaniol accumulation compared with low temperature. In addition, the positive relationship between the size of lesion diameter and the concentrations of neosolaniol and T-2 toxin was observed. T-2 toxin and neosolaniol were found not only in the rotten part, but also in the adjacent asymptomatic tissue, and the concentration showed a trend of decline with increase in distance from the rotten parts. The study will be useful not only for apple post-harvest storage and reduce the health risk of exposure to the trichothecenes of T-2 toxin and neosolaniol, but also provide baseline information for the introduction of EU-limits for T-2 toxin and neosolaniol.
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Activation of phenylpropanoid pathway and PR of potato tuber against Fusarium sulphureum by fungal elicitor from Trichothecium roseum.
World Journal of Microbiology & Biotechnology, 2016Co-Authors: Lu Yan, Yi Wang, Xiao Liu, Shen KepingAbstract:The induced resistance of potato tuber (Solanum tuberosum cv. Xindaping) tissue against Fusarium sulphureum by a fungal elicitor from the incompatible pathogen Trichothecium roseum and its possible mechanism were studied. The results showed that the lesion development of the wound-inoculated potato tuber was significantly reduced by treatment with the fungal elicitor from T. roseum (P
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damage to Trichothecium roseum caused by sodium silicate is independent from ph
Canadian Journal of Microbiology, 2016Co-Authors: Yang Bi, Shenggui Zhang, Yongcai Li, Yi Wang, Alejandro CalderonurreaAbstract:Trichothecium roseum is one of the most important postharvest pathogens in arid and semiarid regions. Sodium silicate (NaSi) and environmental pH have significant inhibitory effects on fungal growth. However, no study has addressed the relationship of NaSi and pH in combination and the effects on T. roseum. In this work, we showed that spore germination, germ tube elongation, and mycelial growth of T. roseum were significantly inhibited by various NaSi concentrations, which had corresponding increasing pHs. Furthermore, these NaSi solutions showed a much greater impact than did pH treatments alone. The pathogenicity of NaSi-treated conidia on a model assay (conidia-inoculated apple fruit) was dramatically reduced, whereas no changes of pathogenicity were evident for the corresponding pH (various sodium hydroxide (NaOH) solutions) treatments. Fluorescent microscopy, using propidium iodide staining, showed damage of the plasma membranes of T. roseum conidia treated with both NaSi and NaOH, although the dama...
Mubashar Raza - One of the best experts on this subject based on the ideXlab platform.
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Trichothecium roseum causes fruit rot of tomato orange and apple in pakistan
Plant Disease, 2014Co-Authors: M I Hamid, Muzammil Hussain, Muhammad Usman Ghazanfar, Mubashar RazaAbstract:During a field survey of greenhouses and fresh markets in 2013, fruits of tomato, oranges, and apples exhibited rot symptoms with white mycelial growth and salmon-color sporulation in the vicinity of Sargodha city (32°5′1″ N, 72°40′16″ E), Pakistan. Diseased fruit samples were collected in plastic bags and taken to laboratory on ice for further diagnosis. Diseased fruits were observed under a stereo microscope and single spores were removed using an inoculating needle. Isolation from single spores showed pink to white colonies on potato dextrose agar (PDA) containing hyaline, 2-celled, ellipsoid to pyriform conidia (17 to 24 × 7 to 11 μm) with slanting and truncate basal mark and produced in clusters. Conidiophores were branched (105 to 254 × 2 to 4 μm) and hyphae were hyaline (3 to 5 μm in diameter). These characteristics of the fungus were similar to Trichothecium roseum (Pers.) as reported by Inacio et al. (1). Genomic DNA was extracted by using CTAB buffer from a single pure colony of one isolate of t...
Dov Prusky - One of the best experts on this subject based on the ideXlab platform.
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early defense responses involved in mitochondrial energy metabolism and reactive oxygen species accumulation in harvested muskmelons infected by Trichothecium roseum
Journal of Agricultural and Food Chemistry, 2019Co-Authors: Yang Bi, Shenge Li, Zhong Zhang, Dov PruskyAbstract:Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H2O2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase. However, the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transpor...
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Early Defense Responses Involved in Mitochondrial Energy Metabolism and Reactive Oxygen Species Accumulation in Harvested Muskmelons Infected by Trichothecium roseum
2019Co-Authors: Liang Lyu, Huali Xue, Zhong Zhang, Dov PruskyAbstract:Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H2O2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H+-ATPase, and Ca2+-ATPase. However, the activities of Ca2+-ATPase and H+-ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transporter and mitochondria dysfunction. Taken together, our results suggest that mitochondria play crucial roles in the early defense responses of muskmelons against T. roseum infection through regulation of ROS production and energy metabolism
Louise Larissa - One of the best experts on this subject based on the ideXlab platform.
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producao e armazenamento de Trichothecium roseum para uso como biopesticida production and storage of Trichothecium roseum to use as biopesticide
2010Co-Authors: Genuino Negri, Louise LarissaAbstract:Trichothecium roseum is a potential antagonistic fungus to control peach Brown rot reducing the pathogen infection and disease damage. The current experiment aimed at developing a methodology for production of the antagonist Trichothecium roseum in different substrates, and to test its viability when stored in several temperatures. The substrates used were brown rice, rice hulls, sorghum grain and wheat grain boiled to tap water for 5, 10 and 20 min and not boiled, with and without added of nutrients with four repetitions. The evaluation of the stored inoculum viability was tested in temperatures: -4, 4, 15, 25, 35 o C and room temperature, for 90 days. The analysis of variance indicated significant interactions among the substrates, the time of boiling and the types of treatment with or without the use of nutrients. The rice and wheat substrates without PD (potato + dextrose) nutrients addition were the more efficients in the production of the antagonist, demonstrating that these do not require the addition of nutrients for multiplication of the fungus. To maintain the viability of the inoculum The best storage condition was at temperature -4 o C, where of rates in viability of the conidia were 74% at 30 days, 44% at 60 days and 32% at 90 days; this temperature can easily be obtained in freezers; therefore, future users do not need to make high investments in equipment for the conservation of the antagonist.
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nota cientifica crescimento micelial de monilinia fructicola e Trichothecium roseum em diferentes temperaturas e sensibilidade do antagonista a fungicidas e fosfitos 1 mycelial growth of monilinia fructicola and Trichothecium roseum in several temper
2007Co-Authors: Luciene Martins Moreira, Louise LarissaAbstract:Brown rot is an important disease economically in peachtree cultivation and is mainly controlled by fungicides but biological control has been proposed as an alternative. Thus the present study aimed to investigate the growth of Monilinia fructicola and Trichothecium roseum in different temperatures and also establish the effect of fungicides and phosphites on the mycelia growth inhibition of antagonist. The mycelia growth of T. roseum and five M. fructicola isolates was assessed in the BDA culture medium at 5, 10, 15, 20, 25, 30 and 35 °C. The sensitivity of the T. roseum antagonist isolate was defined in culture medium with 1, 10 and 100 mg L -1 of the iprodione, azoxystrobin, captan, mancozebe, tebuconazole, iminoctadine tris albesilate fungicides and Ca and K phosphites. Results showed that the optimum, maximum and minimum temperatures for T. roseum mycelial growth were 20 to 25, 36 and 4,9 o C, respectively and for the pathogen were from 20 to 25, 35 and from 4,9 to 2,5 °C. The study of the effects of the chemical products showed that at the concentration of 100 mg L-1 the phosphites and the captan, azoxystrobin and iminoctadine fungicides exercised less inhibition of the antagonist, suggesting that these products may be used in an integrated control program for brown rot.
