The Experts below are selected from a list of 282 Experts worldwide ranked by ideXlab platform
William E Aldeen - One of the best experts on this subject based on the ideXlab platform.
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use of a single slide Trichrome Stained concentrate for the detection of intestinal parasites Stained concentration procedure for ova and parasites
American Journal of Clinical Pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a eupricpolyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
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Use of a single slide Trichrome-Stained concentrate for the detection of intestinal parasites. Stained concentration procedure for ova and parasites.
American journal of clinical pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a cupric-polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
Devon C Hale - One of the best experts on this subject based on the ideXlab platform.
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use of a single slide Trichrome Stained concentrate for the detection of intestinal parasites Stained concentration procedure for ova and parasites
American Journal of Clinical Pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a eupricpolyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
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Use of a single slide Trichrome-Stained concentrate for the detection of intestinal parasites. Stained concentration procedure for ova and parasites.
American journal of clinical pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a cupric-polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
Michael P Carty - One of the best experts on this subject based on the ideXlab platform.
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insights into uv induced apoptosis ultrastructure Trichrome Stain and spectral imaging
Micron, 2002Co-Authors: Marian L Miller, Anastasia Andringa, Kathleen Dixon, Michael P CartyAbstract:Abstract Nuclear substructures associated with apoptosis in HeLa cells have been examined using light-microscopic morphometry, Trichrome Staining, spectral imaging and transmission electron microscopy. This detailed analysis reveals several sites where alterations in the normal cellular ultrastructure occur during apoptotic progression. To correlate these ultrastructural changes with the underlying molecular processes, we have characterized and quantified apoptotic cell morphology with and without inhibition of two caspases, which are key effectors of the apoptotic program. Using this analysis, early apoptotic events included: (a) the segregation of nucleolar components, a diminished granular component, and a reduction in number but increase in size of fibrillar centers, (b) an increase in the number of cytoplasmic ribosomes and (c) a very minimal increase in the amount of peripherally condensed DNA. Apoptosis progressed with: (a) an increase in the number of perichromatin granules and perichromatin fibrils, (b) a reduction in number of interchromatin granule centers concomitant with an increase in their size, (c) partial digestion and circumferential condensation of the DNA at the nuclear membrane and (d) rounding of the cytoplasm with an increase in organellar density and shrinkage in cell size. Endstage apoptotic cells showed: (a) one (or two) very large pools of incompletely digested DNA, (b) one (or two) very large interchromatin granule centers, (c) an increased number of perichromatin granules which were distanced from DNA and often closely apposed to the nucleolus, (d) formation of unusually condensed, highly coiled perinucleolar bodies and (e) blebbing of highly dense cytoplasm. In HeLa cells treated with UV and inhibitors of caspase 1 and 3, the length of time from early apoptosis to the formation of apoptotic bodies was greatly extended. Inhibiting caspase activity: (a) prevented the pooling of DNA, (b) retarded the formation of large interchromatin granule centers, (c) increased the number of perichromatin granules, (d) produced disassembly of the nucleolus, (e) prevented the formation of highly coiled perinucleolar bodies, and (f) caused vacuolization in the cell center and a unipolar blebbing of the cytoplasm. Spectral imaging in conjunction with serial section electron microscopy confirmed the Staining specificities of the condensed DNA, of the large condensed interchromatin granule centers, and of the nucleoli. The results indicate that the interface between the components of the chromatin domain and the interchromatin space is a critical site of caspase activity in apoptosis, and precedes other events such as internucleosomal DNA degradation.
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Insights into UV-induced apoptosis: ultrastructure, Trichrome Stain and spectral imaging.
Micron (Oxford England : 1993), 2002Co-Authors: Marian L Miller, Anastasia Andringa, Kathleen Dixon, Michael P CartyAbstract:Nuclear substructures associated with apoptosis in HeLa cells have been examined using light-microscopic morphometry, Trichrome Staining, spectral imaging and transmission electron microscopy. This detailed analysis reveals several sites where alterations in the normal cellular ultrastructure occur during apoptotic progression. To correlate these ultrastructural changes with the underlying molecular processes, we have characterized and quantified apoptotic cell morphology with and without inhibition of two caspases, which are key effectors of the apoptotic program. Using this analysis, early apoptotic events included: (a) the segregation of nucleolar components, a diminished granular component, and a reduction in number but increase in size of fibrillar centers, (b) an increase in the number of cytoplasmic ribosomes and (c) a very minimal increase in the amount of peripherally condensed DNA. Apoptosis progressed with: (a) an increase in the number of perichromatin granules and perichromatin fibrils, (b) a reduction in number of interchromatin granule centers concomitant with an increase in their size, (c) partial digestion and circumferential condensation of the DNA at the nuclear membrane and (d) rounding of the cytoplasm with an increase in organellar density and shrinkage in cell size. Endstage apoptotic cells showed: (a) one (or two) very large pools of incompletely digested DNA, (b) one (or two) very large interchromatin granule centers, (c) an increased number of perichromatin granules which were distanced from DNA and often closely apposed to the nucleolus, (d) formation of unusually condensed, highly coiled perinucleolar bodies and (e) blebbing of highly dense cytoplasm. In HeLa cells treated with UV and inhibitors of caspase 1 and 3, the length of time from early apoptosis to the formation of apoptotic bodies was greatly extended. Inhibiting caspase activity: (a) prevented the pooling of DNA, (b) retarded the formation of large interchromatin granule centers, (c) increased the number of perichromatin granules, (d) produced disassembly of the nucleolus, (e) prevented the formation of highly coiled perinucleolar bodies, and (f) caused vacuolization in the cell center and a unipolar blebbing of the cytoplasm. Spectral imaging in conjunction with serial section electron microscopy confirmed the Staining specificities of the condensed DNA, of the large condensed interchromatin granule centers, and of the nucleoli. The results indicate that the interface between the components of the chromatin domain and the interchromatin space is a critical site of caspase activity in apoptosis, and precedes other events such as internucleosomal DNA degradation.
Charlotte K Ryan - One of the best experts on this subject based on the ideXlab platform.
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should Trichrome Stain be used on all post liver transplant biopsies with hepatitis c virus infection to estimate the fibrosis score
Liver Transplantation, 2008Co-Authors: David Tretheway, Ashok Jain, Randi Lapoint, Mohammed S. Orloff, Patricia Milot, Adel Bozorgzadeh, Rajeev Sharma, Charlotte K RyanAbstract:Recurrent hepatitis C is virtually universal after liver transplantation; however, an individual patient’s clinical course and disease burden are highly variable and difficult to predict. The fibrosis score determined on posttransplant biopsies appears to be a sensitive and specific marker of disease progression and severity. Currently, the fibrosis score is determined from hematoxylin and eosin (H&E)–Stained tissue sections supplemented by variable use of Trichrome Stain or other connective tissue–specific Stains. In this study, we compare the fibrosis score on H&E Stain with that obtained with Trichrome Stain in posttransplant liver biopsies of patients with hepatitis C. A total of 197 liver biopsies from 105 allograft patients with hepatitis C were reviewed. The mean fibrosis stage was 1.0 1.25 with H&E Stain versus 1.69 1.42 with Trichrome Stain (P 0.00001). The Trichrome staging score was higher in 53.3%, lower in 3%, and the same in 43.7%. The fibrosis stage was raised by 2 or more points in 17.8% and elevated into a bridging category in 14.7%. No significant differences in clinical and laboratory levels were measured in patients with higher fibrosis scores. In conclusion, the hepatic fibrosis score is significantly underestimated by H&E Stain in the posttransplant setting in patients with hepatitis C. The fibrosis stage may be an indicator of significant liver damage in these patients. Accuracy of its determination may be most easily facilitated by employment of a connective tissue Stain. Liver Transpl 14:695-700, 2008. © 2008 AASLD.
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should Trichrome Stain be used on all post liver transplant biopsies with hepatitis c virus infection to estimate the fibrosis score
Liver Transplantation, 2008Co-Authors: David Tretheway, Ashok Jain, Randi Lapoint, Mohammed S. Orloff, Patricia Milot, Adel Bozorgzadeh, Rajeev Sharma, Charlotte K RyanAbstract:Recurrent hepatitis C is virtually universal after liver transplantation; however, an individual patient’s clinical course and disease burden are highly variable and difficult to predict. The fibrosis score determined on posttransplant biopsies appears to be a sensitive and specific marker of disease progression and severity. Currently, the fibrosis score is determined from hematoxylin and eosin (H&E)–Stained tissue sections supplemented by variable use of Trichrome Stain or other connective tissue–specific Stains. In this study, we compare the fibrosis score on H&E Stain with that obtained with Trichrome Stain in posttransplant liver biopsies of patients with hepatitis C. A total of 197 liver biopsies from 105 allograft patients with hepatitis C were reviewed. The mean fibrosis stage was 1.0 1.25 with H&E Stain versus 1.69 1.42 with Trichrome Stain (P 0.00001). The Trichrome staging score was higher in 53.3%, lower in 3%, and the same in 43.7%. The fibrosis stage was raised by 2 or more points in 17.8% and elevated into a bridging category in 14.7%. No significant differences in clinical and laboratory levels were measured in patients with higher fibrosis scores. In conclusion, the hepatic fibrosis score is significantly underestimated by H&E Stain in the posttransplant setting in patients with hepatitis C. The fibrosis stage may be an indicator of significant liver damage in these patients. Accuracy of its determination may be most easily facilitated by employment of a connective tissue Stain. Liver Transpl 14:695-700, 2008. © 2008 AASLD.
James R Kucera - One of the best experts on this subject based on the ideXlab platform.
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use of a single slide Trichrome Stained concentrate for the detection of intestinal parasites Stained concentration procedure for ova and parasites
American Journal of Clinical Pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a eupricpolyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
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Use of a single slide Trichrome-Stained concentrate for the detection of intestinal parasites. Stained concentration procedure for ova and parasites.
American journal of clinical pathology, 1996Co-Authors: Devon C Hale, James R Kucera, Karen C. Carroll, William E AldeenAbstract:The detection and identification of intestinal parasites was studied using a single slide of a Trichrome-Stained concentrate made from a cupric-polyvinyl alcohol (PVA)-preserved specimen. When saline was used in place of formalin in the concentration procedure, it was possible to adequately Stain the concentrate with a modified-Trichrome Stain. This was compared to a more traditional two-slide ova and parasite (O&P) procedure. Of the 1,000 specimens evaluated, a total of 261 parasites were detected using the single slide, modified Trichrome-Stained concentrate compared to 249 from the formalin/Hemo-De wet mount and 226 from the unconcentrated Trichrome Stain. The detection rate of the single slide method was comparable to the combined detection rate of the two-slide method. The saline/Hemo-De concentration procedure has the advantage of using nontoxic saline in place of formaldehyde. This new method is more cost-effective because it requires the processing and evaluation of only one slide.
